WT5BZ] The polymorphic Msp I site in the 5th exon of the bovine growth hormone(bGH)gene was detected by using PCR procedure.A 340bp fragment enclosing the sequence from 2165 position to the 2504 position was amplified...WT5BZ] The polymorphic Msp I site in the 5th exon of the bovine growth hormone(bGH)gene was detected by using PCR procedure.A 340bp fragment enclosing the sequence from 2165 position to the 2504 position was amplified using upper primer 5’GGGCAGATCCTCAAGCAGA 3’ and lower primer 5’ATCCTCCCCCTTGCTGTCC 3’.Genomic DNA samples were from Simmental(n=126),Luxi(n=14),Piemontese(n=8),crossbreed of Piemontese and Nanyang(n=44),Nanyang(n=17),and Hanwoo( Bos taurus koreanae, n=108).PCR products digested with Msp I were separated by electrophoresis in 8% polyacrylamide gel and detected by silver staining.Only a 340bp amplified product could be observed when a Msp I site was absent(UU type).The amplified product,which had a MspⅠ site,showed 245bp and 95bp fragments(CC type).In Simmental and Luxi breeds,only CC was detected.The two polymorphisms(CC and CU)were typed in Piemontese and Nanyang.UU types were observed in Hanwoo breed only.The allele frequencies of C and U were:Piemontese:0.875,0.125;crossbreed of Piemontese and Nanyang:0.818,0 182;Nanyang:0.941,0.059;Hanwoo:0.894,0.106,respectively.PCR products of CC and UU types was sequenced by use of ABI PRISM TM 377 DNA sequencer.A C→T transition was revealed at 2258 position.展开更多
介绍了一种基于MSP430单片机的低功耗油田注水流量控制系统,该系统以阀门为控制对象,利用MSP430的Tim er A模式,产生PWM输出控制信号,实现电机的PWM控制,从而通过控制注水阀门的开度,调节注水流量。采用模糊自调整PID控制,实现油田注水...介绍了一种基于MSP430单片机的低功耗油田注水流量控制系统,该系统以阀门为控制对象,利用MSP430的Tim er A模式,产生PWM输出控制信号,实现电机的PWM控制,从而通过控制注水阀门的开度,调节注水流量。采用模糊自调整PID控制,实现油田注水流量的闭环调节,同时,利用GPRS模块将数据远距离传送给PC机,实现远程控制,在低功耗设计上,除了在硬件上选用低功耗芯片外,还利用软件设计进一步降低了功耗,仿真结果表明:采用PWM和模糊自调整PID控制流量具有良好的动态、稳态性,从而证明了这种设计的合理性和优越性。展开更多
文摘WT5BZ] The polymorphic Msp I site in the 5th exon of the bovine growth hormone(bGH)gene was detected by using PCR procedure.A 340bp fragment enclosing the sequence from 2165 position to the 2504 position was amplified using upper primer 5’GGGCAGATCCTCAAGCAGA 3’ and lower primer 5’ATCCTCCCCCTTGCTGTCC 3’.Genomic DNA samples were from Simmental(n=126),Luxi(n=14),Piemontese(n=8),crossbreed of Piemontese and Nanyang(n=44),Nanyang(n=17),and Hanwoo( Bos taurus koreanae, n=108).PCR products digested with Msp I were separated by electrophoresis in 8% polyacrylamide gel and detected by silver staining.Only a 340bp amplified product could be observed when a Msp I site was absent(UU type).The amplified product,which had a MspⅠ site,showed 245bp and 95bp fragments(CC type).In Simmental and Luxi breeds,only CC was detected.The two polymorphisms(CC and CU)were typed in Piemontese and Nanyang.UU types were observed in Hanwoo breed only.The allele frequencies of C and U were:Piemontese:0.875,0.125;crossbreed of Piemontese and Nanyang:0.818,0 182;Nanyang:0.941,0.059;Hanwoo:0.894,0.106,respectively.PCR products of CC and UU types was sequenced by use of ABI PRISM TM 377 DNA sequencer.A C→T transition was revealed at 2258 position.
文摘介绍了一种基于MSP430单片机的低功耗油田注水流量控制系统,该系统以阀门为控制对象,利用MSP430的Tim er A模式,产生PWM输出控制信号,实现电机的PWM控制,从而通过控制注水阀门的开度,调节注水流量。采用模糊自调整PID控制,实现油田注水流量的闭环调节,同时,利用GPRS模块将数据远距离传送给PC机,实现远程控制,在低功耗设计上,除了在硬件上选用低功耗芯片外,还利用软件设计进一步降低了功耗,仿真结果表明:采用PWM和模糊自调整PID控制流量具有良好的动态、稳态性,从而证明了这种设计的合理性和优越性。
