Inter Simple Sequence Repeats （ISSR） markers were used to assess genetic diversity within and among populations of dwarf mountain pine （Pinus rnugo Turra） growing in the Tatra National Park （UNESCO Biosphere Rese...Inter Simple Sequence Repeats （ISSR） markers were used to assess genetic diversity within and among populations of dwarf mountain pine （Pinus rnugo Turra） growing in the Tatra National Park （UNESCO Biosphere Reserve） in Southern Poland （Central Europe）. The analyzed population belongs to two different geobotanical sub-districts： the Western and High Tatras. The level of genetic diversity assessed in this study for P. mugo is generally comparable to that reported for the other pine species in the Pinaceae family assessed by ISSR markers, especially with respect to Nei＇s genetic diversity and the percentage of polymorphic bands. Bayesian analysis clustered the analyzed populations into two groups, corresponding to their geobotanical locations in the Tatras. Significant divergence between the two genetical clusters was supported by the results of Analysis of Molecular Variance （AMOVA）. According to the Mantel test, there was no correlation between the genetic distance and the geographical distance. The present study confirms the existence of two genetically distinct dusters of P. mugo populations in the Tatra Mountains. The observed high population-genetic differentiation of P.mugo in the Tatras could be attributed to several genetic, environmental and historical factors occurring in this mountain area.展开更多
Artificial hybridization experiment with the <i>Pinus</i> <i>sylvestris</i> and <i>Pinus</i> <i>mugo</i> putative hybrid individuals and their parents revealed a selecti...Artificial hybridization experiment with the <i>Pinus</i> <i>sylvestris</i> and <i>Pinus</i> <i>mugo</i> putative hybrid individuals and their parents revealed a selective nature of their crossability conditioned by the identical chloroplast DNA (cpDNA) haplotypes of the crossed individuals. Efficiency of hybridization between hybrid individual H1 of <i>P</i>. <i>sylvestris</i> haplotype and <i>P</i>. <i>sylvestris</i> paternal tree was relatively high, as evidenced by 78.03% of filled seeds. On the contrary, the crossing of H1 individual with <i>P</i>. <i>mugo</i> was unsuccessful. Likewise, the crossability of the H2 individual of <i>P</i>. <i>mugo</i> haplotype with the paternal tree <i>P</i>. <i>mugo</i> resulted in 31.15% of filled seeds. A relatively high crossability was characteristic also for the H1 and H2 hybrid trees of different cpDNA haplotypes. A conclusion has been drawn postulating weakened reproduction barrier between hybrid swarm individuals and the paternal species <i>P</i>. <i>sylvestris</i> and <i>P</i>. <i>mugo</i>. Differentiation of the putatively hybrid individuals from the parental species is accompanied by the variation in cpDNA inheritance.展开更多
基金supported by Research Grant No. NN304060339 from the Ministry of Science and Higher Education of Poland
文摘Inter Simple Sequence Repeats （ISSR） markers were used to assess genetic diversity within and among populations of dwarf mountain pine （Pinus rnugo Turra） growing in the Tatra National Park （UNESCO Biosphere Reserve） in Southern Poland （Central Europe）. The analyzed population belongs to two different geobotanical sub-districts： the Western and High Tatras. The level of genetic diversity assessed in this study for P. mugo is generally comparable to that reported for the other pine species in the Pinaceae family assessed by ISSR markers, especially with respect to Nei＇s genetic diversity and the percentage of polymorphic bands. Bayesian analysis clustered the analyzed populations into two groups, corresponding to their geobotanical locations in the Tatras. Significant divergence between the two genetical clusters was supported by the results of Analysis of Molecular Variance （AMOVA）. According to the Mantel test, there was no correlation between the genetic distance and the geographical distance. The present study confirms the existence of two genetically distinct dusters of P. mugo populations in the Tatra Mountains. The observed high population-genetic differentiation of P.mugo in the Tatras could be attributed to several genetic, environmental and historical factors occurring in this mountain area.
文摘Artificial hybridization experiment with the <i>Pinus</i> <i>sylvestris</i> and <i>Pinus</i> <i>mugo</i> putative hybrid individuals and their parents revealed a selective nature of their crossability conditioned by the identical chloroplast DNA (cpDNA) haplotypes of the crossed individuals. Efficiency of hybridization between hybrid individual H1 of <i>P</i>. <i>sylvestris</i> haplotype and <i>P</i>. <i>sylvestris</i> paternal tree was relatively high, as evidenced by 78.03% of filled seeds. On the contrary, the crossing of H1 individual with <i>P</i>. <i>mugo</i> was unsuccessful. Likewise, the crossability of the H2 individual of <i>P</i>. <i>mugo</i> haplotype with the paternal tree <i>P</i>. <i>mugo</i> resulted in 31.15% of filled seeds. A relatively high crossability was characteristic also for the H1 and H2 hybrid trees of different cpDNA haplotypes. A conclusion has been drawn postulating weakened reproduction barrier between hybrid swarm individuals and the paternal species <i>P</i>. <i>sylvestris</i> and <i>P</i>. <i>mugo</i>. Differentiation of the putatively hybrid individuals from the parental species is accompanied by the variation in cpDNA inheritance.
