Mugwort has many traditional uses around the world. The Chumash Indians of California use it to treat imbalances that women may suffer such as premenstrual syndrome, dysmenorrhea and menopausal symptoms. The plant con...Mugwort has many traditional uses around the world. The Chumash Indians of California use it to treat imbalances that women may suffer such as premenstrual syndrome, dysmenorrhea and menopausal symptoms. The plant contains a sesquiterpene that appears to work through a serotonergic mechanism and may be beneficial for women. Mugwort therapy is safer for menopausal women than hormone replacement therapy. Children affected by attention deficit hyperactivity disorder benefit from mugwort therapy. There is no doubt that mugwort therapy is safer for these children than methylphenidate or amphetamine.展开更多
White mugwort (Artemisia lactiflora Wall.) is known as a medicinal herb containing phytochemicals with antioxidant and bio-activity.The optimal concentration of ethanol was investigated for extracting polyphenols from...White mugwort (Artemisia lactiflora Wall.) is known as a medicinal herb containing phytochemicals with antioxidant and bio-activity.The optimal concentration of ethanol was investigated for extracting polyphenols from the fresh aerial part and dried powder of white mugwort as expressed by total phenolic content (TPC),phenolic compounds,antioxidant,anti-inflammatory,and anti-adipogenic activity.Among various ethanol concentrations (0,25,50 or 95%) used in the extraction process,the freeze-dried extract from fresh aerial part (FE) extracted with 95% ethanol expressed the highest antioxidant activity (DPPH radical scavenging IC50 of 0.817 ± 0.06 μl/mg dry weight (DW) and FRAP value of 11.40 ± 0.86 mmol Fe(II)/g DW) and the highest TPC (61.46 ± 3.09 mgGAE/g DW).While,the freeze-dried extract from dried powder (PE) exhibited the prominent antioxidant properties (DPPH radical scavenging IC50 of 0.975 ± 0.03 μl/mg DW and FRAP value of 07.60 ± 0.22 mmol Fe(II)/g DW),TPC (48.11 ± 1.45 mgGAE/g DW) when extracted with 50% ethanol.Seven phenolic compounds—two phenolic acids (gallic acid and tannic acid) and five flavonoids (apigenin,isoquercetin,quercetin,rutin,and catechin) were identified in FE and PE using HPLC-DAD/MS.The inflammatory genes were down-regulated in lipopolysaccharide-stimulated THP-1 monocytes treated with PE,suggesting anti-inflammatory activity.Lipid accumulation in 3T3-L1 adipocytes treated with FE at 60 μ gGAE/mL decreased to 12.8%,while the decrease of 36.8% was detected in PE.Hence,flavonoids were suggested to be responsible for the stronger bioactivity of PE over FE.Moreover,this work originally revealed the investigation of the anti-inflammatory and anti-adipogenic activity of white mugwort on THP-1 and 3T3-L1 cells.展开更多
文摘Mugwort has many traditional uses around the world. The Chumash Indians of California use it to treat imbalances that women may suffer such as premenstrual syndrome, dysmenorrhea and menopausal symptoms. The plant contains a sesquiterpene that appears to work through a serotonergic mechanism and may be beneficial for women. Mugwort therapy is safer for menopausal women than hormone replacement therapy. Children affected by attention deficit hyperactivity disorder benefit from mugwort therapy. There is no doubt that mugwort therapy is safer for these children than methylphenidate or amphetamine.
基金funded by Thailand Researchers and Research for Industry Grants(RRi)(Grant No.PHD61I0001)。
文摘White mugwort (Artemisia lactiflora Wall.) is known as a medicinal herb containing phytochemicals with antioxidant and bio-activity.The optimal concentration of ethanol was investigated for extracting polyphenols from the fresh aerial part and dried powder of white mugwort as expressed by total phenolic content (TPC),phenolic compounds,antioxidant,anti-inflammatory,and anti-adipogenic activity.Among various ethanol concentrations (0,25,50 or 95%) used in the extraction process,the freeze-dried extract from fresh aerial part (FE) extracted with 95% ethanol expressed the highest antioxidant activity (DPPH radical scavenging IC50 of 0.817 ± 0.06 μl/mg dry weight (DW) and FRAP value of 11.40 ± 0.86 mmol Fe(II)/g DW) and the highest TPC (61.46 ± 3.09 mgGAE/g DW).While,the freeze-dried extract from dried powder (PE) exhibited the prominent antioxidant properties (DPPH radical scavenging IC50 of 0.975 ± 0.03 μl/mg DW and FRAP value of 07.60 ± 0.22 mmol Fe(II)/g DW),TPC (48.11 ± 1.45 mgGAE/g DW) when extracted with 50% ethanol.Seven phenolic compounds—two phenolic acids (gallic acid and tannic acid) and five flavonoids (apigenin,isoquercetin,quercetin,rutin,and catechin) were identified in FE and PE using HPLC-DAD/MS.The inflammatory genes were down-regulated in lipopolysaccharide-stimulated THP-1 monocytes treated with PE,suggesting anti-inflammatory activity.Lipid accumulation in 3T3-L1 adipocytes treated with FE at 60 μ gGAE/mL decreased to 12.8%,while the decrease of 36.8% was detected in PE.Hence,flavonoids were suggested to be responsible for the stronger bioactivity of PE over FE.Moreover,this work originally revealed the investigation of the anti-inflammatory and anti-adipogenic activity of white mugwort on THP-1 and 3T3-L1 cells.
