The ratcheting response of 316 stainless steel samples at the vicinity of notch roots under single-and multi-step loading conditions is evaluated.Multi-step tests were conducted to examine local ratcheting at differen...The ratcheting response of 316 stainless steel samples at the vicinity of notch roots under single-and multi-step loading conditions is evaluated.Multi-step tests were conducted to examine local ratcheting at different low–high–high and high–low–low loading sequences.The stress levels over loading steps and their sequences highly influenced ratcheting magnitude and rate.The change of stress level from low to high promoted ratcheting over proceeding cycles while ratcheting strains dropped in magnitude for opposing sequence where stress level dropped from high to low.Local ratcheting strain values at the vicinity of notch root were found noticeably larger than nominal ratcheting values measured at farer distances from notch edge through use of strain gauges.Ratcheting values in both mediums of local and nominal were promoted as notch diameter increased.To assess progressive ratcheting response and stress relaxation concurrently,the Ahmadzadeh-Varvani(A-V)kinematic hardening rule was coupled with Neuber’s rule enabling to calculate local stress at notch root of steel samples.Local stress/strain values were progressed at notch root over applied asymmetric stress cycles resulting in ratcheting buildup through A-V model.The relaxation of stress values at a given peak-valley strain event was governed through the Neuber’s rule.Experimental ratcheting data were found agreeable with those predicted through the coupled framework.展开更多
Transposable elements (TEs) are selfish genetic elements whose self-replication is contrasted by the host genome. In this context, host reproductive strategies are predicted to impact on both TEs load and activity. ...Transposable elements (TEs) are selfish genetic elements whose self-replication is contrasted by the host genome. In this context, host reproductive strategies are predicted to impact on both TEs load and activity. The presence and insertion distribution of the non- LTR retrotransposon R2 was here studied in populations of the strictly bisexual Bacillus grandii maretimi and of the obligatory parthenogenetic Bacillus atticus atticus. Further- more, data were also obtained from the offspring of selected B. a. atticus females. At the population level, the gonochoric B. g. maretimi showed a significantly higher R2 load than the obligatory parthenogenetic B. a. atticus. The comparison with bisexual and unisexual Bacillus rossius populations showed that their values were higher than those recorded for B. a. atticus and similar, or even higher, than those of B. g. maretimi. Consistently, an R2 load reduction is scored in B. a. atticus offspring even if with a great variance. On the whole, data here produced indicate that in the obligatory unisexual B. a. atticus R2 is active and that mechanisms of molecular turnover are effective. Furthermore, progeny analyses show that, at variance of the facultative parthenogenetic B. rossius, the R2 activ- ity is held at a lower rate. Modeling parental-offspring inheritance, suggests that in B. a. atticus recombination plays a major role in eliminating insertions rather than selection, as previously suggested for unisexual B. rossius progeny, even if in both cases a high variance is observed. In addition to this, mechanisms of R2 silencing or chances of clonal selection cannot be ruled out.展开更多
基金Authors wish to acknowledge the financial support by the Natural Sciences and Engineering Research Council(NSERC)of Canada.
文摘The ratcheting response of 316 stainless steel samples at the vicinity of notch roots under single-and multi-step loading conditions is evaluated.Multi-step tests were conducted to examine local ratcheting at different low–high–high and high–low–low loading sequences.The stress levels over loading steps and their sequences highly influenced ratcheting magnitude and rate.The change of stress level from low to high promoted ratcheting over proceeding cycles while ratcheting strains dropped in magnitude for opposing sequence where stress level dropped from high to low.Local ratcheting strain values at the vicinity of notch root were found noticeably larger than nominal ratcheting values measured at farer distances from notch edge through use of strain gauges.Ratcheting values in both mediums of local and nominal were promoted as notch diameter increased.To assess progressive ratcheting response and stress relaxation concurrently,the Ahmadzadeh-Varvani(A-V)kinematic hardening rule was coupled with Neuber’s rule enabling to calculate local stress at notch root of steel samples.Local stress/strain values were progressed at notch root over applied asymmetric stress cycles resulting in ratcheting buildup through A-V model.The relaxation of stress values at a given peak-valley strain event was governed through the Neuber’s rule.Experimental ratcheting data were found agreeable with those predicted through the coupled framework.
文摘Transposable elements (TEs) are selfish genetic elements whose self-replication is contrasted by the host genome. In this context, host reproductive strategies are predicted to impact on both TEs load and activity. The presence and insertion distribution of the non- LTR retrotransposon R2 was here studied in populations of the strictly bisexual Bacillus grandii maretimi and of the obligatory parthenogenetic Bacillus atticus atticus. Further- more, data were also obtained from the offspring of selected B. a. atticus females. At the population level, the gonochoric B. g. maretimi showed a significantly higher R2 load than the obligatory parthenogenetic B. a. atticus. The comparison with bisexual and unisexual Bacillus rossius populations showed that their values were higher than those recorded for B. a. atticus and similar, or even higher, than those of B. g. maretimi. Consistently, an R2 load reduction is scored in B. a. atticus offspring even if with a great variance. On the whole, data here produced indicate that in the obligatory unisexual B. a. atticus R2 is active and that mechanisms of molecular turnover are effective. Furthermore, progeny analyses show that, at variance of the facultative parthenogenetic B. rossius, the R2 activ- ity is held at a lower rate. Modeling parental-offspring inheritance, suggests that in B. a. atticus recombination plays a major role in eliminating insertions rather than selection, as previously suggested for unisexual B. rossius progeny, even if in both cases a high variance is observed. In addition to this, mechanisms of R2 silencing or chances of clonal selection cannot be ruled out.