In an automatic bobbin management system that simultaneously detects bobbin color and residual yarn,a composite texture segmentation and recognition operation based on an odd partial Gabor filter and multi-color space...In an automatic bobbin management system that simultaneously detects bobbin color and residual yarn,a composite texture segmentation and recognition operation based on an odd partial Gabor filter and multi-color space hierarchical clustering are proposed.Firstly,the parameter-optimized odd partial Gabor filter is used to distinguish bobbin and yarn texture,to explore Garbor parameters for yarn bobbins,and to accurately discriminate frequency characteristics of yarns and texture.Secondly,multi-color clustering segmentation using color spaces such as red,green,blue(RGB)and CIELUV(LUV)solves the problems of over-segmentation and segmentation errors,which are caused by the difficulty of accurately representing the complex and variable color information of yarns in a single-color space and the low contrast between the target and background.Finally,the segmented bobbin is combined with the odd partial Gabor’s edge recognition operator to further distinguish bobbin texture from yarn texture and locate the position and size of the residual yarn.Experimental results show that the method is robust in identifying complex texture,damaged and dyed bobbins,and multi-color yarns.Residual yarn identification can distinguish texture features and residual yarns well and it can be transferred to the detection and differentiation of complex texture,which is significantly better than traditional methods.展开更多
Conventional color-printing systems often use inks of three hues, such as CMY, CMYK and CMYKLcLm, but in order to obtain more realistic color reproductions, the ink set of more than three hues has been adopted by some...Conventional color-printing systems often use inks of three hues, such as CMY, CMYK and CMYKLcLm, but in order to obtain more realistic color reproductions, the ink set of more than three hues has been adopted by some color-printing systems. It is difficult, however, to model the composed color with the multiple inks when the number of the output ink hues exceeds three due to the none-unique mapping between the color spaces of the CIE Lab and the multi-color printing device. In this paper, we propose a fine color-printing method for multi-color printing device with the ink set of more than three hues. The proposed approach has good color expression ability and provides fine control of the printed color. By dividing the output color space into several subspaces, our method allows one-to-one mapping between the standard color space and the multi-color output color space. It has been proved effective when applied to the digital inkjet printer—Mutoh8000.展开更多
We study theoretically intense terahertz radiation from multi-color laser pulse with uncommon frequency ratios. Com- paring the two-color laser scheme, of which the uncommon frequency ratio should be set to be a speci...We study theoretically intense terahertz radiation from multi-color laser pulse with uncommon frequency ratios. Com- paring the two-color laser scheme, of which the uncommon frequency ratio should be set to be a specific value, we show that by using multi-color harmonic laser pulses as the first pump component, the lasers as the second pump component can be adjusted in a continuous frequency range. Moreover, these multi-color laser pulses can effectively modulate and enhance the terahertz radiation, and the terahertz yield increases with the increase of the wavelength of the uncommon pump com- ponent and is stable to the laser relative phase. Finally, we utilize the electron densities and velocities of ionization events to illustrate the physical mechanism of the intense terahertz generation.展开更多
Manipulating and real-time monitoring of neuronal activities with cell-type specificity and precise spatiotemporal resolution during animal behavior are fundamental technologies for exploring the functional connectivi...Manipulating and real-time monitoring of neuronal activities with cell-type specificity and precise spatiotemporal resolution during animal behavior are fundamental technologies for exploring the functional connectivity, information transmission, and physiological functions of neural circuits in vivo. However, current techniques for optogenetic stimulation and neuronal activity recording mostly operate independently. Here, we report an all-fiber-transmission photometry system for simultaneous optogenetic manipulation and multi-color recording of neuronal activities and the neurotransmitter release in a freely moving animal. We have designed and manufactured a wavelength-independent multi-branch fiber bundle to enable simultaneous optogenetic manipulation and multi-color recording at different wavelengths. Further, we combine a laser of narrow linewidth with the lock-in amplification method to suppress the optogenetic stimulation-induced artifacts and channel crosstalk. We show that the collection efficiency of our system outperforms a traditional epi-fluorescence system. Further, we demonstrate successful recording of dynamic dopamine(DA) responses to unexpected rewards in the nucleus accumbens(NAc) in a freely moving mouse. We also show simultaneous dual-color recording of neuronal Ca2+ signals and DA dynamics in the NAc upon delivering an unexpected reward and the simultaneous optogenetic activating at dopaminergic terminals in the same location. Thus, our multi-function fiber photometry system provides a compatible, efficient, and flexible solution for neuroscientists to study neural circuits and neurological diseases.展开更多
Carbon dots(CDs)with aggregation-induced emission(AIE)have sparked significant interest in multidimensional anti-counterfeiting due to their exceptional fluorescence properties.However,the preparation of AIE CDs with ...Carbon dots(CDs)with aggregation-induced emission(AIE)have sparked significant interest in multidimensional anti-counterfeiting due to their exceptional fluorescence properties.However,the preparation of AIE CDs with multicolor solid-state fluorescence remains a formidable challenge due to its complicated construction.In the present work,a novel class of multicolor AIE CDs(M-CDs)were fabricated using selected precursor(salicylic acid,thiosalicylic acid,and 2,2'-dithiodibenzoic acid),with an eco-friendly,low-cost one-pot solvothermal method.In the dilute organic solution,M-CDs manifested blue emission,but upon aggregation in the presence of water,the red,yellow,green,and blue emissions were displayed due to the AIE effect.Structural analysis,coupled with theoretical calculations,revealed that the increase in the size of sp2 domains would lower the Eg and cause a red-shift emission wavelength.Significantly,the continuous emission of M-CDs from blue to red can be utilized as ink for multimode printing,enabling the creation of a variety of school badges and quick response codes.These findings hold promising implications for multi-information encryption applications.展开更多
Programming microscopic assembly mode to control macroscopic property is an attractive research objective.In particular,controlling molecular assembly to control fluorescence is of considerable interest for developing...Programming microscopic assembly mode to control macroscopic property is an attractive research objective.In particular,controlling molecular assembly to control fluorescence is of considerable interest for developing smart fluorescent materials.Herein,a color-tunable supramolecular emissive system was developed based on cucurbit[8]uril mediated host-guest assembly.Chemical designing for the molecular structures with minimized change resulted in different assembly modes and hence generating distinctive fluorescence,including green,yellow and orange with the addition of cucurbit[n]uril.Taking advantage of this feature,the advanced information encryption material(4D code)with multiple encryption levels and time-dependent encryption feature was developed.Such a code was dynamic on time scale,generating a series of 3D codes with time.The encrypted information only can be recognized by integrating time-coursed codes.This work provides a new insight for designing intelligent fluorescent materials for information encryption with high level of security.展开更多
Frequency conversion based on three-wave mixing is a critical nonlinear optic application,extending the frequency range of existing lasers and realizing frequency-transduced detectors in a wavelength range that lacks ...Frequency conversion based on three-wave mixing is a critical nonlinear optic application,extending the frequency range of existing lasers and realizing frequency-transduced detectors in a wavelength range that lacks an effective detector.Phase matching is vital for effective frequency conversion.The advantages of quasi-phase matching(QPM)over birefringent phase matching are a lack of walk-off effect,a maximum nonlinear coefficient,and phase matching in the entire transparency window.Herein,using different types and orders of QPM,four kinds of effective frequency doubling processes are realized in a periodically poled potassium titanyl phosphate(KTP)crystal with a single period,and three kinds of frequency doubling processes are experimentally verified.We also show a feasible way to construct an RGB color generator based on two different QPM processes.This study significantly expands the feasible frequency conversion of existing lasers to different wavelengths,providing an effective method for multi-color laser generation based on periodically poled KTP crystals.展开更多
Mechano-luminescence(ML) has been found diverse applications such as stress sensing,3-D signature,energy harvesting,and anti-counterfeiting,due to the unique properties of the corresponding ML phosphors performing in-...Mechano-luminescence(ML) has been found diverse applications such as stress sensing,3-D signature,energy harvesting,and anti-counterfeiting,due to the unique properties of the corresponding ML phosphors performing in-situ and real-time response to mechanical stimulus.Trap-controlled ML materials realize reproducible,quantitative mechano-optical conversion resulting from the released carriers for the trap-mediated charge storage and release.Herein,modulating traps matters the controllable ML behavior for their promising applications.In this work,a deep trap distributed ranging from 0.9 to 1,3 eV is realized consecutively for the introduction of Sm^(3+)ions into LaGaO_(3) matrix,which contributes to the red emission at 600 nm under mechanical stimuli.Moreover,tunable ML emission modulated from 600to 545 nm is successfully achieved for the codopant of Tb^(3+)ions.It is unambiguously indicated that the released carriers from the trap sharing rather than an additional constructed trap contribute to the modulated ML color.Accordingly,multi-mode anti-counterfeiting and encryption are achieved with the as-explored Sm^(3+),Tb^(3+)co-activated ML phosphor.展开更多
Fluorescence imaging in the second near-infrared window(NIR-II,900–1880 nm)with less scattering background in biological tissues has been combined with the confocal microscopic system for achieving deep in vivo imagi...Fluorescence imaging in the second near-infrared window(NIR-II,900–1880 nm)with less scattering background in biological tissues has been combined with the confocal microscopic system for achieving deep in vivo imaging with high spatial resolution.However,the traditional NIR-IIfluorescence confocal microscope with separate excitation focus and detection pinhole makes it possess low confocal e±ciency,as well as di±cultly to adjust.Two types of upgraded NIR-IIfluorescence confocal microscopes,sharing the same pinhole by excitation and emission focus,leading to higher confocal e±ciency,are built in this work.One type is-ber-pinhole-based confocal microscope applicable to CW laser excitation.It is constructed forfluorescence intensity imaging with large depth,high stabilization and low cost,which could replace multiphotonfluorescence microscopy in some applications(e.g.,cerebrovascular and hepatocellular imaging).The other type is air-pinhole-based confocal microscope applicable to femtosecond(fs)laser excitation.It can be employed not only for NIR-IIfluorescence intensity imaging,but also for multi-channelfluorescence lifetime imaging to recognize different structures with similarfluorescence spectrum.Moreover,it can be facilely combined with multiphotonfluorescence microscopy.A single fs pulsed laser is utilized to achieve up-conversion(visible multiphotonfluorescence)and down-conversion(NIR-II one-photonfluorescence)excitation simultaneously,extending imaging spectral channels,and thus facilitates multi-structure and multi-functional observation.展开更多
【目的】叶面积指数(leaf area index,LAI)是表征作物长势、光合、蒸腾的重要指标。论文旨在研究不同生育期、多生育期无人机多光谱数据棉花LAI估测模型,明确不同生育期间棉花LAI估测模型变化规律,为实时掌握棉花长势并因地制宜进行田...【目的】叶面积指数(leaf area index,LAI)是表征作物长势、光合、蒸腾的重要指标。论文旨在研究不同生育期、多生育期无人机多光谱数据棉花LAI估测模型,明确不同生育期间棉花LAI估测模型变化规律,为实时掌握棉花长势并因地制宜进行田间科学管理提供依据。【方法】利用大疆精灵4多光谱无人机获取棉花现蕾期、初花期、结铃期、吐絮期多光谱图像和RGB图像。选用归一化差植被指数(NDVI)、绿度归一化差植被指数(GNDVI)、归一化差红边指数(NDRE)、叶片叶绿素指数(LCI)、优化的土壤调节植被指数(OSAVI)5种多光谱指数和修正红绿植被指数(MGRVI)、红绿植被指数(GRVI)、绿叶指数(GLA)、超红指数(EXR)、大气阻抗植被指数(VARI)5种颜色指数分别建立棉花各生育期及棉花生长多生育期数据集合,结合打孔法获取地面LAI实测数据,使用机器学习算法中偏最小二乘(PLSR)、岭回归(RR)、随机森林(RF)、支持向量机(SVM)、神经网络(BP)构建棉花LAI预测模型。【结果】覆膜棉花LAI随着生育期的变化呈现先增长后下降的趋势,现蕾期、初花期、结铃期内侧棉花叶面积指数均值均显著大于外侧(P<0.05);选择的指数在各时期彼此间均呈显著相关(P<0.05),总体而言,多光谱指数与颜色指数间的相关性随着生育期的进行而呈现下降趋势,选择的指数在各时期均与棉花LAI相关性显著(P<0.05),多光谱指数相关系数介于0.35—0.85,颜色指数相关系数介于0.49—0.71,相关系数绝对值较大的指数多为多光谱指数,颜色指数与棉花LAI的相关系数绝对值较小;估测模型性能结果显示棉花各生育期模型中多光谱指数优于颜色指数,且各指数模型预测性能随着生育期的变化呈现一定规律性,NDVI是预测棉花LAI的最优指数。从模型结果上看,RF模型和BP模型在各生育期下获得了较高的估计精度。初花期LAI反演模型精度最高,最优模型验证集R2为0.809,MAE为0.288,NRMSE为0.120。多生育期最优模型验证集R2为0.386,MAE为0.700,NRMSE为0.198。【结论】棉花内外侧LAI在现蕾期、初花期、结铃期存在显著差异。在各生育期中,RF和BP模型是预测棉花LAI较优模型。NDVI在各指数中表现最好,是预测棉花LAI的最优指数。多生育期模型效果较单生育期明显下降,最优指数为GNDVI,最优模型为BP。本研究中预测棉花LAI的最优窗口期是初花期。研究结果可为无人机遥感监测棉花LAI提供理论依据和技术支持。展开更多
To identify alien chromosomes in recipient progenies and to analyze genome components in polyploidy, a genomic in situ hybridization (GISH) technique that is suitable for cotton was developed using increased stringe...To identify alien chromosomes in recipient progenies and to analyze genome components in polyploidy, a genomic in situ hybridization (GISH) technique that is suitable for cotton was developed using increased stringency conditions. The increased stringency conditions were a combination of the four factors in the following optimized state: 100:1 ratio of blocking DNA to probe, 60% formamide wash solution, 43 ℃ temperature wash and a 13 min wash. Under these specific conditions using gDNA from Gossypium sturtianum (C1 C1 ) as a probe, strong hybridization signals were only observed on chromosomes from the C1 genome in somatic cells of the hybrid F1 (G. hirsutum x G. sturtianum) (AtDtC1). Therefore, GISH was able to discriminate parental chromosomes in the hybrid. Further, we developed a multi-color GISH to simultaneously discriminate the three genomes of the above hybrid. The results repeatedly displayed the three genomes, At, Dt, and C1, and each set of chromosomes with a unique color, making them easy to identify. The power of the multi-color GISH was proven by analysis of the hexaploid hybrid F1 (G. hirsutum x G. australe) (AtAtDtDtG2G2). We believe that the powerful multi-color GISH technique could be applied extensively to analyze the genome component in polyploidy and to identify alien chromosomes in the recipient progenies.展开更多
基金Key Research and Development Plan of Shaanxi Province,China(No.2023-YBGY-330)。
文摘In an automatic bobbin management system that simultaneously detects bobbin color and residual yarn,a composite texture segmentation and recognition operation based on an odd partial Gabor filter and multi-color space hierarchical clustering are proposed.Firstly,the parameter-optimized odd partial Gabor filter is used to distinguish bobbin and yarn texture,to explore Garbor parameters for yarn bobbins,and to accurately discriminate frequency characteristics of yarns and texture.Secondly,multi-color clustering segmentation using color spaces such as red,green,blue(RGB)and CIELUV(LUV)solves the problems of over-segmentation and segmentation errors,which are caused by the difficulty of accurately representing the complex and variable color information of yarns in a single-color space and the low contrast between the target and background.Finally,the segmented bobbin is combined with the odd partial Gabor’s edge recognition operator to further distinguish bobbin texture from yarn texture and locate the position and size of the residual yarn.Experimental results show that the method is robust in identifying complex texture,damaged and dyed bobbins,and multi-color yarns.Residual yarn identification can distinguish texture features and residual yarns well and it can be transferred to the detection and differentiation of complex texture,which is significantly better than traditional methods.
基金Project (No. M603034) supported by the Natural Science Foundationof Zhejiang Province, China
文摘Conventional color-printing systems often use inks of three hues, such as CMY, CMYK and CMYKLcLm, but in order to obtain more realistic color reproductions, the ink set of more than three hues has been adopted by some color-printing systems. It is difficult, however, to model the composed color with the multiple inks when the number of the output ink hues exceeds three due to the none-unique mapping between the color spaces of the CIE Lab and the multi-color printing device. In this paper, we propose a fine color-printing method for multi-color printing device with the ink set of more than three hues. The proposed approach has good color expression ability and provides fine control of the printed color. By dividing the output color space into several subspaces, our method allows one-to-one mapping between the standard color space and the multi-color output color space. It has been proved effective when applied to the digital inkjet printer—Mutoh8000.
基金Project supported by the National Natural Science Foundation of China(Grant No.11604205)the Talent Program of Shanghai University of Engineering Science,China
文摘We study theoretically intense terahertz radiation from multi-color laser pulse with uncommon frequency ratios. Com- paring the two-color laser scheme, of which the uncommon frequency ratio should be set to be a specific value, we show that by using multi-color harmonic laser pulses as the first pump component, the lasers as the second pump component can be adjusted in a continuous frequency range. Moreover, these multi-color laser pulses can effectively modulate and enhance the terahertz radiation, and the terahertz yield increases with the increase of the wavelength of the uncommon pump com- ponent and is stable to the laser relative phase. Finally, we utilize the electron densities and velocities of ionization events to illustrate the physical mechanism of the intense terahertz generation.
基金supported by Beijing Municipal Governmentsupported by the National Natural Science Foundation of China(Grant Nos.61890952)the Director Fund of WNLO。
文摘Manipulating and real-time monitoring of neuronal activities with cell-type specificity and precise spatiotemporal resolution during animal behavior are fundamental technologies for exploring the functional connectivity, information transmission, and physiological functions of neural circuits in vivo. However, current techniques for optogenetic stimulation and neuronal activity recording mostly operate independently. Here, we report an all-fiber-transmission photometry system for simultaneous optogenetic manipulation and multi-color recording of neuronal activities and the neurotransmitter release in a freely moving animal. We have designed and manufactured a wavelength-independent multi-branch fiber bundle to enable simultaneous optogenetic manipulation and multi-color recording at different wavelengths. Further, we combine a laser of narrow linewidth with the lock-in amplification method to suppress the optogenetic stimulation-induced artifacts and channel crosstalk. We show that the collection efficiency of our system outperforms a traditional epi-fluorescence system. Further, we demonstrate successful recording of dynamic dopamine(DA) responses to unexpected rewards in the nucleus accumbens(NAc) in a freely moving mouse. We also show simultaneous dual-color recording of neuronal Ca2+ signals and DA dynamics in the NAc upon delivering an unexpected reward and the simultaneous optogenetic activating at dopaminergic terminals in the same location. Thus, our multi-function fiber photometry system provides a compatible, efficient, and flexible solution for neuroscientists to study neural circuits and neurological diseases.
基金the National Natural Science Foundation of China(No.21807085)the Natural Science Foundation Research Project of Shaanxi Province(No.2023-JCYB-087)+2 种基金the Technology Innovation Leading Program of Shaanxi(No.2020QFY07-05)the Innovation Capability Support Program of Shaanxi(No.2022KJXX-88)the fund of Education Department of Shaanxi Province(Program No.Z20230071).
文摘Carbon dots(CDs)with aggregation-induced emission(AIE)have sparked significant interest in multidimensional anti-counterfeiting due to their exceptional fluorescence properties.However,the preparation of AIE CDs with multicolor solid-state fluorescence remains a formidable challenge due to its complicated construction.In the present work,a novel class of multicolor AIE CDs(M-CDs)were fabricated using selected precursor(salicylic acid,thiosalicylic acid,and 2,2'-dithiodibenzoic acid),with an eco-friendly,low-cost one-pot solvothermal method.In the dilute organic solution,M-CDs manifested blue emission,but upon aggregation in the presence of water,the red,yellow,green,and blue emissions were displayed due to the AIE effect.Structural analysis,coupled with theoretical calculations,revealed that the increase in the size of sp2 domains would lower the Eg and cause a red-shift emission wavelength.Significantly,the continuous emission of M-CDs from blue to red can be utilized as ink for multimode printing,enabling the creation of a variety of school badges and quick response codes.These findings hold promising implications for multi-information encryption applications.
基金supported by the National Natural Science Foundation of China(22025503,22220102004)the Shanghai Municipal Science and Technology Major Project(2018SHZDZX03)+3 种基金the Fundamental Research Funds for the Central Universitiesthe Programme of Introducing Talents of Discipline to Universities(B16017)the Science and Technology Commission of Shanghai Municipality(21JC1401700)the Starry Night Science Fund of Zhejiang University Shanghai Institute for Advanced Study(SN-ZJU-SIAS-006)。
文摘Programming microscopic assembly mode to control macroscopic property is an attractive research objective.In particular,controlling molecular assembly to control fluorescence is of considerable interest for developing smart fluorescent materials.Herein,a color-tunable supramolecular emissive system was developed based on cucurbit[8]uril mediated host-guest assembly.Chemical designing for the molecular structures with minimized change resulted in different assembly modes and hence generating distinctive fluorescence,including green,yellow and orange with the addition of cucurbit[n]uril.Taking advantage of this feature,the advanced information encryption material(4D code)with multiple encryption levels and time-dependent encryption feature was developed.Such a code was dynamic on time scale,generating a series of 3D codes with time.The encrypted information only can be recognized by integrating time-coursed codes.This work provides a new insight for designing intelligent fluorescent materials for information encryption with high level of security.
基金supported by the National Natural Science Foundation of China(Nos.92065101 and 11934013)the Space Debris Research Project of China(No.KJSP2020020202)。
文摘Frequency conversion based on three-wave mixing is a critical nonlinear optic application,extending the frequency range of existing lasers and realizing frequency-transduced detectors in a wavelength range that lacks an effective detector.Phase matching is vital for effective frequency conversion.The advantages of quasi-phase matching(QPM)over birefringent phase matching are a lack of walk-off effect,a maximum nonlinear coefficient,and phase matching in the entire transparency window.Herein,using different types and orders of QPM,four kinds of effective frequency doubling processes are realized in a periodically poled potassium titanyl phosphate(KTP)crystal with a single period,and three kinds of frequency doubling processes are experimentally verified.We also show a feasible way to construct an RGB color generator based on two different QPM processes.This study significantly expands the feasible frequency conversion of existing lasers to different wavelengths,providing an effective method for multi-color laser generation based on periodically poled KTP crystals.
基金Project supported by the Yunnan Provincial Natural Science Foundation (202101AT070126)the Research Start-up Fund of Chengdu University of Technology (10912-KYQD2020-08476)+1 种基金Fund of Hubei Key Laboratory of Plasma Chemistry,Advanced Materials (KF202103)Sichuan Natural Science Foundation (2022YFH0108,2022JDJQ0030)。
文摘Mechano-luminescence(ML) has been found diverse applications such as stress sensing,3-D signature,energy harvesting,and anti-counterfeiting,due to the unique properties of the corresponding ML phosphors performing in-situ and real-time response to mechanical stimulus.Trap-controlled ML materials realize reproducible,quantitative mechano-optical conversion resulting from the released carriers for the trap-mediated charge storage and release.Herein,modulating traps matters the controllable ML behavior for their promising applications.In this work,a deep trap distributed ranging from 0.9 to 1,3 eV is realized consecutively for the introduction of Sm^(3+)ions into LaGaO_(3) matrix,which contributes to the red emission at 600 nm under mechanical stimuli.Moreover,tunable ML emission modulated from 600to 545 nm is successfully achieved for the codopant of Tb^(3+)ions.It is unambiguously indicated that the released carriers from the trap sharing rather than an additional constructed trap contribute to the modulated ML color.Accordingly,multi-mode anti-counterfeiting and encryption are achieved with the as-explored Sm^(3+),Tb^(3+)co-activated ML phosphor.
基金supported by National Natural Science Foundation of China(61975172,82001874 and 61735016).
文摘Fluorescence imaging in the second near-infrared window(NIR-II,900–1880 nm)with less scattering background in biological tissues has been combined with the confocal microscopic system for achieving deep in vivo imaging with high spatial resolution.However,the traditional NIR-IIfluorescence confocal microscope with separate excitation focus and detection pinhole makes it possess low confocal e±ciency,as well as di±cultly to adjust.Two types of upgraded NIR-IIfluorescence confocal microscopes,sharing the same pinhole by excitation and emission focus,leading to higher confocal e±ciency,are built in this work.One type is-ber-pinhole-based confocal microscope applicable to CW laser excitation.It is constructed forfluorescence intensity imaging with large depth,high stabilization and low cost,which could replace multiphotonfluorescence microscopy in some applications(e.g.,cerebrovascular and hepatocellular imaging).The other type is air-pinhole-based confocal microscope applicable to femtosecond(fs)laser excitation.It can be employed not only for NIR-IIfluorescence intensity imaging,but also for multi-channelfluorescence lifetime imaging to recognize different structures with similarfluorescence spectrum.Moreover,it can be facilely combined with multiphotonfluorescence microscopy.A single fs pulsed laser is utilized to achieve up-conversion(visible multiphotonfluorescence)and down-conversion(NIR-II one-photonfluorescence)excitation simultaneously,extending imaging spectral channels,and thus facilitates multi-structure and multi-functional observation.
文摘【目的】叶面积指数(leaf area index,LAI)是表征作物长势、光合、蒸腾的重要指标。论文旨在研究不同生育期、多生育期无人机多光谱数据棉花LAI估测模型,明确不同生育期间棉花LAI估测模型变化规律,为实时掌握棉花长势并因地制宜进行田间科学管理提供依据。【方法】利用大疆精灵4多光谱无人机获取棉花现蕾期、初花期、结铃期、吐絮期多光谱图像和RGB图像。选用归一化差植被指数(NDVI)、绿度归一化差植被指数(GNDVI)、归一化差红边指数(NDRE)、叶片叶绿素指数(LCI)、优化的土壤调节植被指数(OSAVI)5种多光谱指数和修正红绿植被指数(MGRVI)、红绿植被指数(GRVI)、绿叶指数(GLA)、超红指数(EXR)、大气阻抗植被指数(VARI)5种颜色指数分别建立棉花各生育期及棉花生长多生育期数据集合,结合打孔法获取地面LAI实测数据,使用机器学习算法中偏最小二乘(PLSR)、岭回归(RR)、随机森林(RF)、支持向量机(SVM)、神经网络(BP)构建棉花LAI预测模型。【结果】覆膜棉花LAI随着生育期的变化呈现先增长后下降的趋势,现蕾期、初花期、结铃期内侧棉花叶面积指数均值均显著大于外侧(P<0.05);选择的指数在各时期彼此间均呈显著相关(P<0.05),总体而言,多光谱指数与颜色指数间的相关性随着生育期的进行而呈现下降趋势,选择的指数在各时期均与棉花LAI相关性显著(P<0.05),多光谱指数相关系数介于0.35—0.85,颜色指数相关系数介于0.49—0.71,相关系数绝对值较大的指数多为多光谱指数,颜色指数与棉花LAI的相关系数绝对值较小;估测模型性能结果显示棉花各生育期模型中多光谱指数优于颜色指数,且各指数模型预测性能随着生育期的变化呈现一定规律性,NDVI是预测棉花LAI的最优指数。从模型结果上看,RF模型和BP模型在各生育期下获得了较高的估计精度。初花期LAI反演模型精度最高,最优模型验证集R2为0.809,MAE为0.288,NRMSE为0.120。多生育期最优模型验证集R2为0.386,MAE为0.700,NRMSE为0.198。【结论】棉花内外侧LAI在现蕾期、初花期、结铃期存在显著差异。在各生育期中,RF和BP模型是预测棉花LAI较优模型。NDVI在各指数中表现最好,是预测棉花LAI的最优指数。多生育期模型效果较单生育期明显下降,最优指数为GNDVI,最优模型为BP。本研究中预测棉花LAI的最优窗口期是初花期。研究结果可为无人机遥感监测棉花LAI提供理论依据和技术支持。
基金the National Natural Science Foundation of China (30571184)Jiangsu Provincial Natural Science Foundation (BK2007166)+3 种基金the Tenth Five-year Plan of the National Key Program (2004BA525B05)the 111 Project(B08025) the Eleventh Five-year Plan of the National Sci-technologicalSupporting Program (2006BAD13B04-1-08)the Changjiang Scholars and Innovative Research Team in University and the Teaching and Research AwardProgram for Outstanding Young Teachers in Higher Education Institutions ofMinistry of Education (MOE), China.
文摘To identify alien chromosomes in recipient progenies and to analyze genome components in polyploidy, a genomic in situ hybridization (GISH) technique that is suitable for cotton was developed using increased stringency conditions. The increased stringency conditions were a combination of the four factors in the following optimized state: 100:1 ratio of blocking DNA to probe, 60% formamide wash solution, 43 ℃ temperature wash and a 13 min wash. Under these specific conditions using gDNA from Gossypium sturtianum (C1 C1 ) as a probe, strong hybridization signals were only observed on chromosomes from the C1 genome in somatic cells of the hybrid F1 (G. hirsutum x G. sturtianum) (AtDtC1). Therefore, GISH was able to discriminate parental chromosomes in the hybrid. Further, we developed a multi-color GISH to simultaneously discriminate the three genomes of the above hybrid. The results repeatedly displayed the three genomes, At, Dt, and C1, and each set of chromosomes with a unique color, making them easy to identify. The power of the multi-color GISH was proven by analysis of the hexaploid hybrid F1 (G. hirsutum x G. australe) (AtAtDtDtG2G2). We believe that the powerful multi-color GISH technique could be applied extensively to analyze the genome component in polyploidy and to identify alien chromosomes in the recipient progenies.