Large-field objective lens for multi-wavelength microscopy at mesoscale and submicron resolution

Conventional microscopes designed for submicron resolution in biological research are hindered by a limited field of view,typically around 1 mm.This restriction poses a challenge when attempting to simultaneously analyze various parts of a sample,such as different brain areas.In addition,conventional objective lenses struggle to perform consistently across the required range of wavelengths for brain imaging in vivo.Here we present a novel mesoscopic objective lens with an impressive field of view of 8 mm,a numerical aperture of 0.5,and a working wavelength range from 400 to 1000 nm.We achieved a resolution of 0.74μm in fluorescent beads imaging.The versatility of this lens was further demonstrated through high-quality images of mouse brain and kidney sections in a wide-field imaging system,a confocal laser scanning system,and a two-photon imaging system.This mesoscopic objective lens holds immense promise for advancing multi-wavelength imaging of large fields of view at high resolution.

True-temperature inversion algorithm for a multi-wavelength pyrometer based on fractional-order particle-swarm optimization

Herein,a method of true-temperature inversion for a multi-wavelength pyrometer based on fractional-order particle-swarm optimization is proposed for difficult inversion problems with unknown emissivity.Fractional-order calculus has the inherent advantage of easily jumping out of local extreme values;here,it is introduced into the particle-swarm algorithm to invert the true temperature.An improved adaptive-adjustment mechanism is applied to automatically adjust the current velocity order of the particles and update their velocity and position values,increasing the accuracy of the true temperature values.The results of simulations using the proposed algorithm were compared with three algorithms using typical emissivity models:the internal penalty function algorithm,the optimization function(fmincon)algorithm,and the conventional particle-swarm optimization algorithm.The results show that the proposed algorithm has good accuracy for true-temperature inversion.Actual experimental results from a rocket-motor plume were used to demonstrate that the true-temperature inversion results of this algorithm are in good agreement with the theoretical true-temperature values.

The Theoretical Analysis of Multi-wavelength Pyrometer:Check and Autosearch for Emissivity General Expression

In general, the dependence of the logarithm of the emissivity upon wavelength is usually used in the treatment of data measured by multi-wavelength pyrometer. In this paper, the suitability of this expression for different materials has been examined. Further more, an effective method for automatically searching the mathematical model between emissivity and wavelength has been procsed. The calculated results show that the accuracy is improved by using this data treatment method.

Terahertz Generation Using Implanted InGaAs Photomixers and Multi-wavelength Quantum Dot Lasers

We report on a study of terahertz(THz) generation using implanted In Ga As photomixers and multi-wavelength quantum dot lasers. We carry out In Ga As materials growth, optical characterization, device design and fabrication, and photomixing experiments. This approach is capable of generating a comb of electromagnetic radiation from microwave to terahertz. For shortening photomixer carrier lifetime, we employ proton implantation into an epitaxial layer of lattice matched In Ga As grown on InP. Under a 1.55 μm multimode In GaAs/In GaAsP quantum dot laser excitation, a frequency comb with a constant frequency spacing of 50 GHz generated on the photomixer is measured, which corresponds to the beats of the laser longitudinal modes. The measurement is performed with a Fourier transform infrared spectrometer. This approach affords a convenient method to achieve a broadband multi-peak coherent THz source.

Multi-wavelength continuous-wave Nd:YVO4 self-Raman laser under in-band pumping

Multi-wavelength continuous-wave self-Raman laser with an a-cut composite YVO4/Nd:YVO4/YVO4 crystal pumped by an 879-nm wavelength-locked laser diode is demonstrated for the first time.Multi-wavelength Raman lasers at 1168.4,1176,1178.7,and 1201.6 nm are achieved by the first Stokes shift of the multi-wavelength fundamental lasers at 1064,1066.7,1073.6,1084,and 1085.6 nm with two Raman shifts of 890 and 816 cm^-1.A maximum Raman output power of 2.56 W is achieved through the use of a 20-mm-long composite crystal,with a corresponding optical conversion efficiency of 9.8%.The polarization directions of different fundamental and Raman lasers are investigated and found to be orthogonalπandσpolarizations.These orthogonally polarized multi-wavelength lasers with small wavelength separation pave the way to the development of a potential laser source for application in spectral analysis,laser radar and THz generation.

Monte-Carlo Studies and Computer Modelling of Multi-wavelength Pyrometry

Ｍｏｎｔｅ－ＣａｒｌｏＳｔｕｄｉｅｓａｎｄＣｏｍｐｕｔｅｒＭｏｄｅｌｌｉｎｇｏｆＭｕｌｔｉ－ｗａｖｅｌｅｎｇｔｈＰｙｒｏｍｅｔｒｙ￥ＰＥＩＹｕｅ；ＤＡＩＪｉｎｇｍｉｎ；ＣＨＵＺａｉｘｉａｎｇ(裴越，戴景民，褚载祥)（Ｄｅｐｔ．ｏｆＰｒｅｃｉｓｅＩｎｓｔ...

LOW FLYING HEIGHT MEASUREMENT WITH MULTI-WAVELENGTH INTERFEROMETRY

A method for measurement of ultra-low flying height in head-disk spacing is described. Three different wavelengths are selected out from white light by filters to measure the spacing simultaneously. Besides solving the ambiguity problem, a more reliable result is achieved by using weighted average of measurement results from three different wavelengths, where the weight is dependent upon spacing. Fringe-bunching correction algorithm （FBC） and spot-tilling technique are adopted to suppress calibration and random errors. Moreover, incident bandwidth correction （IBC） method is introduced to compensate the error caused by low monochromaticity of incident light. Based on dynamic flying height tester （DFHT Ⅱ）, with the redesigned of photo-electric conversion and signal acquirement module, an instrument has been developed. And comparing the experimental data from the instrument with those from a KLA-FHT D6, the discrepancy is less than 5%. It indicates that the instrument is suitable to perform ultra-low flying height measurement and satisfies the reauirement of magnetic heads manufacturing.

Multi-wavelength laser active coherent combination

A seed laser oscillating at different frequencies is proved to have the potential to mitigate the stimulated Brillouin scattering（SBS） effect in a fiber amplifier,which may increase the emission power of a coherent beam combination（CBC） system greatly.In this study,a basic mathematical model describing the multi-wavelength CBC is proposed on the fundamentals of CBC.A useful method for estimating the combination effect and analysing the feasibility and the validity of the multi-wavelength coherent combination is provided.In the numerical analysis,accordant results with four-wavelength four-channel CBC experiments are obtained.Through calculations of some examples with certain spectra,the unanticipated excellent combination effect with a few frequencies involved is explained,and the dependence of the combination effect on the variance of the amplifier chain length and the channel number is clarified.

Development of Multi-Wavelength Spectral-Correction Method

This investigation developed a new method for determining metal complex's property containing the stepwise real absorptivity(epsilon) and stability constant (K-m). The correction equation of the multi-wavelength spectral absorption was established for the simultaneous determination of various complexes to give high accuracy for trace analysis. This method was more acceptable in theory and simpler in operation than the classical methods.

Turbidity measurement based on a multi-wavelength spectral sensor

A method of measuring turbidity based on a multi-wavelength spectral sensor is proposed by using SFH4737 broad-band infrared LED,a multi-wavelength spectral sensor and independently developed data processing software.Combining multiple wavelength data from the sensor,the unitary and multivariate fitting models were constructed to investigate the relationship among light intensity information,absorbance and turbidity,respectively.The turbidity of the actual water bodies was measured separately by using proposed method and a commercially visible spectrophotometer.The independent-samples T test(p>0.05)showed that there was no significant difference between the method in this paper and the standard assay method.The method is simple and inexpensive,and can be applied to the rapid detection of water turbidity,providing a new way of industrial online measurement.

Switching,explosion,and chaos of multi-wavelength soliton states in ultrafast fiber lasers

Because of the complexity and difficulty of realizing a multi-wavelength soliton state,reports on its internal dynamic characteristics are scarce.In this study,the switching and periodic soliton explosion processes of the multi-wavelength soliton state in a negative dispersion passively mode-locked fiber laser are realized.The generation of the multi-wavelength soliton state undergoes the process of noise,oscillation,and stable mode-locking,and the splitting and annihilation of solitons with different group velocities directly impact the generation and disappearance of three wavelengths.Positive and negative dispersion lead to different group velocities of solitons.The presence and displacement of solitons with different group velocities cause soliton collisions,which lead to soliton explosions.A soliton experiences relative phase oscillation,chaos,and oscillation,as well as convergence and separation before and after an explosion.With an increase in parameters related to pump power,single-soliton oscillation,multi-wavelength solitons,and chaos are found in experiments and simulations,proving the relevance and reliability between simulation and experimental results.This work promotes the dynamical study of multi-soliton collisions in nonlinear science and the development of chaos theory in multi-comb lasers.

HPLC多波长切换指纹图谱结合化学计量学研究白术土炒前后成分变化规律

目的 建立测定白术、土白术水溶性和脂溶性成分的高效液相色谱-二极管阵列检测器(HPLC-DAD)多波长切换指纹图谱,结合化学计量学研究白术土炒前后成分变化规律。方法 建立白术、土白术水溶性及脂溶性成分的HPLC-DAD多波长切换指纹图谱测定方法,测定17批白术和17批土白术的HPLC指纹图谱,采用相似度评价、聚类分析、OPLS-DA分析进行统计分析。结果 白术指纹图谱中共标定9个共有峰,土白术指纹图谱中共标定10个共有峰。指认了新绿原酸、绿原酸、白术内酯Ⅰ、白术内酯Ⅱ、白术内酯Ⅲ及苍术酮6个成分,土炒后1号峰、4号峰(白术内酯Ⅲ)、8号峰(白术内酯Ⅱ)峰面积增加,2号峰(新绿原酸)、3号峰(绿原酸)、5号峰、6号峰、7号峰、9号峰(白术内酯Ⅰ)、10号峰(苍术酮)土炒后峰面积均降低。结论 所建立指纹图谱方法能够系统地分析白术土炒前后化学成分的变化规律,可为进一步规范白术土炒工艺,制定土白术专属性质量标准,研究土白术炮制原理提供实验基础。

恒星活动与系外宜居行星探索卫星——“紫瞳”科学卫星

生命从哪里来?人类在宇宙中是否独一无二?这些都是各国科学界研究的重大科学问题。恒星辐射与活动性是决定行星宜居性的关键因素。本文提出的科学卫星“紫瞳”,瞄准了系外宜居行星探测等重大科学问题,首次在近紫外-可见光双波段开展恒星活动观测与系外行星搜寻,并期望在“另一个地球”搜寻上取得重大突破。“紫瞳”载荷为4个共视场(10°×10°)的30 cm口径望远镜,等效口径50 cm。“紫瞳”主要科学目标包括:对1000多万颗恒星开展活动性监测,揭示恒星活动性规律;发现2000多颗系外行星候选体,揭示热行星的大气特征;发现红矮星周围的“另一个地球”。此外,“紫瞳”在太阳系小天体探测、暂现源监测、超大质量黑洞搜寻和刻画、引力波对应体搜寻等时域天文学领域也发挥着重要作用,帮助理解宇宙中极端高能事件的物理机制。

多波长与横模可切换掺镱光纤激光器的研究

设计并制备了一种具有环形掺镱纤芯的光纤波导结构,并提出了一种基于该环芯掺镱光纤和少模光纤布拉格光栅的多波长与横模可切换光纤激光器。在这种光纤激光器中,只需简单调整腔内的偏振控制器,就可以实现单波长和双波长的激光稳定振荡。当激光器在单波长下工作时,可以切换两种横模输出,包括LP01模和LP11模,两种模式都获得了小于0.08 nm的3 dB窄线宽和大于45 dB的高边模抑制比,中心波长和光强的波动分别为±0.01 nm和±1 dB。试验表明:该环芯掺镱光纤有助于LP11模获得的增益高于LP01模,LP01模式激光的斜率效率为34.34%,而LP11模式激光的斜率效率高达49.09%。该光纤激光器可以在模分复用系统和激光加工等领域发挥重要的作用。

一种多波长激光器的多模式驱动研究

介绍了一种多波长半导体激光器驱动系统的设计,该系统采用了压控电流源与PicoLAS模块混合驱动的方法,具有效率高、体积小、电路简单可靠、易于系统集成等优点,为半导体激光器的驱动设计提供了一种选择和参考。

基于多波长干涉的瞬态轮廓检测Mirau显微镜

针对单波长Mirau干涉显微镜存在测量范围小和2π模糊等问题,提出了多波长Mirau偏振干涉显微镜,以实现微观轮廓的大动态范围测量的和表面粗糙度等显微结构的瞬态检测。系统利用R、G、B三个单色LED光源实现多波长干涉;利用彩色偏振相机获取瞬态相移干涉条纹图,降低实时瞬态检测中复杂的环境扰动影响;利用线栅偏振片调节条纹对比度,满足不同被测对象的反射率检测要求。为验证系统方案的可行性,经过系统误差补偿校准后测量标称值为1.9939μm的标准微米台阶,结果与标称值的偏差约为5.4 nm。利用该方法与Wyko干涉仪对金刚石车削凸面反射镜表面微观轮廓和表面粗糙度进行测量,表面粗糙度测量结果均方根值偏差为3.7 nm,验证了该系统可实现高精度的大动态范围测量。

Recent progress in multi-wavelength fiber lasers:principles,status,and challenges

In recent years,multi-wavelength fiber lasers play a significant role in plenty of fields,ranging from optical communications to mechanical processing and laser biomedicine,owing to their high beam quality,low cost,and excellent heat dissipation properties.Benefitting from increasing maturity of optical elements,the multi-wavelength fiber laser has made rapid developments.In this review,we summarize and analyze diverse implementation methods covering continuous wave and pulsed fiber lasers at room temperature conditions:inserting an optical filter device and intensity-dependent loss structure in the resonant cavity,and applying ultrafast nonlinear optical response of materials and a dual-cavity structure.Finally,future challenges and perspectives of the multi-wavelength fiber laser are discussed and addressed.

Ultra-long-period grating-based multi-wavelength ultrafast fiber laser [Invited]

We propose and demonstrate the cascaded multi-wavelength mode-locked erbium-doped fiber laser(EDFL) based on ultralong-period gratings(ULPGs) for the first time, to the best of our knowledge.Study found that the ULPG can be used as both a mode-locker for pulse shaping and a comb filter for multi-wavelength generation simultaneously.Using the dual-function of ULPG, three-, four-, five-, six-, and seven-wavelength mode-locked pulses are obtained in EDFL, seven of which are the largest number of wavelengths up to now.For the four-wavelength soliton pulses, their pulse width is about 7.8 ps.The maximum average output power and slope efficiency of these pulses are 8.4 m W and 2.03%, respectively.Besides the conventional pulses, hybrid soliton pulses composed of a four-wavelength pulse and single soliton are also observed.Finally, the effect of cavity dispersion on the multi-wavelength mode-locked pulses is also discussed.Our findings indicate that apart from common sensing and filtering, the ULPG may also possess attractive nonlinear pulse-shaping property for ultrafast photonics application.

5G电源模组高精度3D结构光测量技术

提出了多视角大景深高精度3D视觉传感技术。运用高清数字光处理(DLP)结构光投影技术和高速高分辨工业相机,从板级集成电路设计、控制驱动软件研发、3D点云重建与处理算法等方面深入研究,自主研发亚微米级/低成本多视角大景深高精度3D视觉测量技术。主要包括:采用高分辨率、高帧率的工业摄像机,实现高精度的三维重建;采用多波长相位扩展法,实现高精度的相位计算;利用多工业摄像机解决视场隐藏,扩大了三维重构视场,最终实现了0.48μm Z轴的重复精度。

A Raman laser system for multi-wavelength satellite laser ranging

In order to develop a laser system for multi-wavelength satellite laser ranging, the joint group of the Shanghai Astronomical Observatory and the Czech Technical University has studied the conversion efficiency of the Raman-shifting beam and its spatial characteristics. We adopted a 0.53 μm laser with pulse width of 35 ps and peak energy of 35 mJ, the second harmonic of a Nd:YAG actively and passively mode-locked laser, to pump a one-meter-long Raman tube which is full of H2 with high pressure at the Prague-based laboratory. We get the first Stokes laser (0.68 μm): 7 mJ (single pulse) with beam divergence of 40″ (arcsecond) and spatial wobbling of less than 4″; and the first anti-Stokes laser (0.43 μm): 2 mJ (single pulse) with divergence of 56″ and spatial wobbling of less than 4″. The emitting beam from the Raman cell also includes 0.53 μm: 10 mJ (single pulse) with divergence of 40″ and spatial wobbling of less than 7″. According to the radar link equation and based on the above obtained multi-wavelength’s energy, we can estimate the detection probabilities for three colors respectively. It is shown by the result that the developed multi-wavelength Raman laser system has the capability of satellite ranging. The Raman laser system will be installed at the laser station in Shanghai Astronomical Observatory to research the multi-wavelength satellite laser ranging.