BACKGROUND: Although the hepatoma-specific band of gamma-glutamyltransferase ( GGT ) is a highly sensitive marker in diagnosis of hepatocellular carcinoma, the kine- tic expression and the early alterations of GGT in ...BACKGROUND: Although the hepatoma-specific band of gamma-glutamyltransferase ( GGT ) is a highly sensitive marker in diagnosis of hepatocellular carcinoma, the kine- tic expression and the early alterations of GGT in the deve- lopment of hepatoma remain unclear. In this study, we in- vestigated the expression and the alterations of GGT multi- ple molecular forms in hepatotumorigenesis. METHODS: The expression of GGT in a chemically in- duced hepatocarcinogenesis model was examined by giving 0. 05% of 2-fluoenylacetamide in diet for 12 weeks. The ex- pression levels of total RNA and GGT, and the changes of liver pathology, GGT multiple molecular forms and sugar- chain heterogeneity were investigated at the different stages of rat hepatoma development. RESULTS: Pathological examination and biochemical ana- lysis found that liver GGT was over-expressed and secreted into blood during canceration. Serum total GGT and liver GGT specific activities (IU/g) including soluble and mem- brane-combined GGT were significantly higher (P <0.05) in experimental groups than those in control group, respec- tively. A highly positive correlation was found between to- tal GGT activities and total RNA levels (r =0.90, P <0.05) of the liver. Both were higher six weeks later than before. Con A-non-reactive-GGT was increased consistantly dur- ing the development of rat hepatoma. GGT multiple mo- lecular forms in the liver and sera of experimental rats showed that fetal liver-type GGT bands were associated with the development of hepatoma. CONCLUSIONS: Fetal liver-type GGT in sera and the liver of rats is closely related to hepatotumorigenesis. It can be used as a sensitive enzymatic marker for the early diagnosis of liver cancer.展开更多
We propose and simulate a method for generating a three-dimensional （3D） optical cage in the vicinity of focus by focusing a double-ring shaped radially and azimuthally polarized beam. Our study shows that the combi...We propose and simulate a method for generating a three-dimensional （3D） optical cage in the vicinity of focus by focusing a double-ring shaped radially and azimuthally polarized beam. Our study shows that the combination of an inner ring with an azimuthally polarized field and an outer ring with a radially polarized field and a phase factor can produce an optical cage with a dark region enclosed by higher intensity. The shape of the cage can be tailored by appropriately adjusting the parameters of double-mode beams. Furthermore, multiple 3D optical cages can be realized by applying the shift theorem of the Fourier transform and macro-pixel sampling algorithm to a double-ring shaped radially and azimuthally polarized beam.展开更多
基金This study was supported by a grant from Key Projects of Medical Sciences Department of Health , Jiangsu Province, China ( RC2003100 ).
文摘BACKGROUND: Although the hepatoma-specific band of gamma-glutamyltransferase ( GGT ) is a highly sensitive marker in diagnosis of hepatocellular carcinoma, the kine- tic expression and the early alterations of GGT in the deve- lopment of hepatoma remain unclear. In this study, we in- vestigated the expression and the alterations of GGT multi- ple molecular forms in hepatotumorigenesis. METHODS: The expression of GGT in a chemically in- duced hepatocarcinogenesis model was examined by giving 0. 05% of 2-fluoenylacetamide in diet for 12 weeks. The ex- pression levels of total RNA and GGT, and the changes of liver pathology, GGT multiple molecular forms and sugar- chain heterogeneity were investigated at the different stages of rat hepatoma development. RESULTS: Pathological examination and biochemical ana- lysis found that liver GGT was over-expressed and secreted into blood during canceration. Serum total GGT and liver GGT specific activities (IU/g) including soluble and mem- brane-combined GGT were significantly higher (P <0.05) in experimental groups than those in control group, respec- tively. A highly positive correlation was found between to- tal GGT activities and total RNA levels (r =0.90, P <0.05) of the liver. Both were higher six weeks later than before. Con A-non-reactive-GGT was increased consistantly dur- ing the development of rat hepatoma. GGT multiple mo- lecular forms in the liver and sera of experimental rats showed that fetal liver-type GGT bands were associated with the development of hepatoma. CONCLUSIONS: Fetal liver-type GGT in sera and the liver of rats is closely related to hepatotumorigenesis. It can be used as a sensitive enzymatic marker for the early diagnosis of liver cancer.
基金supported in part by the National Natural Science Foundation of China(Nos.91750202,11530046,and 11474156)the National Key R&D Program of China(No.2017YFA0303700)+1 种基金the Collaborative Innovation Center of Advanced Microstructures of Chinathe Collaborative Innovation Center of Solid-State Lighting and Energy-Saving Electronics of China
文摘We propose and simulate a method for generating a three-dimensional （3D） optical cage in the vicinity of focus by focusing a double-ring shaped radially and azimuthally polarized beam. Our study shows that the combination of an inner ring with an azimuthally polarized field and an outer ring with a radially polarized field and a phase factor can produce an optical cage with a dark region enclosed by higher intensity. The shape of the cage can be tailored by appropriately adjusting the parameters of double-mode beams. Furthermore, multiple 3D optical cages can be realized by applying the shift theorem of the Fourier transform and macro-pixel sampling algorithm to a double-ring shaped radially and azimuthally polarized beam.
