Clinical Effects of Intensive Insulin Therapy Treating Traumatic Shock Combined with Multiple Organ Dysfunction Syndrome

The therapeutic effects of intensive insulin therapy in treatment of traumatic shock combined with multiple organ dysfunction syndrome （MODS） were investigated. A total of 114 patients with traumatic shock combined with MODS were randomly divided into two groups： control group （n=56） treated with conventional therapy, and intensive insulin therapy group （n=58） treated with conventional therapy plus continuous insulin pumping to control the blood glucose level at range of 4.4-6.1 mmol/L. White blood cells （WBC） counts, prothrombin time （PT）, serum creatinine （SCr）, alanine aminotransferase （ALT）, serum albumin and PaO2 were measured before and at the day 1, 3, 5, 7 and 14 after treatment. The incidence of gastrointestinal dysfunction, the incidence of MODS, hospital stay and the mortality were also observed and compared. After intensive insulin therapy, the WBC counts, SCr, ALT and PT were significantly reduced （P0.05）, but the level of serum albumin was significantly increased （P0.05） at the day 3, 5, 7 and 14. In the meantime, the PaO2 was significantly elevated at the day 3, 5 and 7 （P0.01） after intensive insulin therapy. The incidence of gastrointestinal dysfunction, the incidence of MODS, the length of hospital stay and the mortality were markedly decreased （P0.01）. The results suggest early treatment with intensive insulin therapy is effective for traumatic shock combined with MODS and can decrease the length of hospital stay and the mortality.

Morphological changes in interstitial cells of Cajal in the deep muscular plexus and enteric motor neurons of the intestine in rats with multiple organ dysfunction syndrome

BACKGROUND：Gastrointestinal motility dysfunction in multiple organ dysfunction syndrome （MODS） has been reported to be related to damage to interstitial cells of Cajal （ICC）. In the entedc nervous system, ICC and smooth muscle cells are connected in a network to form a special functional unit. Many gastrointestinal motility dysfunction diseases are associated with damage to this network.OBJECTIVE：To investigate the morphological changes of intestinal ICC, and to explore the mechanisms underlying gastrointestinal motility dysfunction in rats with MODS.DESIGN, TIME AND SE＇I-FING：The randomized, controlled, experiment was performed at the Central Laboratory of the First Affiliated Hospital of Dalian Medical University of China between June 2007 and March 2009.MATERIALS：Escherichia coli （E. colistrain O127 H6） and bovine serum albumin were purchased from Sigma, USA.METHODS：A total of 40 Wistar rats were equally and randomly divided into MODS group and control group. Suspension of E. coil strain O127 H6 containing BaSO4 and saline were sterilely injected into the abdominal cavity of rats in the MODS and control groups, respectively.MAIN OUTCOME MEASURES：Immunohistochemical double-staining and confocal laser scanning microscopy were used to observe the morphological changes in intestinal cholinergic nerves and ICC in the deep muscular plexus network. Electron microscopy was employed to evaluate the ultrastructural features of ICC in the deep muscular plexus of rats with MODS.RESULTS：Compared with the control group, the distributions and densities of cholinergic/nitrergic newes and ICC in the deep muscular plexus were significantly decreased in the MODS group （P 〈 0.01）. The enteric nerve-ICC network were disrupted.CONCLUSION：There is ultrastructural injury in the ICC in the deep muscular plexus and enteric nerves of the intestine in rats with MODS, which may be associated with the dysmotility of the gastrointestinal tract in MODS.

Application of bedside continuous blood purification in patients with multiple organ dysfunction syndromes

BACKGROUND:The complications of systemic inflammatory response syndrome(SIRS)include acute lung injury,acute kidney injury,shock,and multiple organ dysfunction syndrome(MODS).In recent years,how to clear inflammatory mediators has become a hot topic in critical care medicine.Researchers hypothesize that continuous blood purification(CBP) can effectively eliminate a variety of inflammatory mediators which participate in the occurrence of MODS and adjust the immune imbalance.This study aimed to observe the effects of CBP in MODS patients.METHODS:In this retrospective clinical study,a total of 38 MODS patients,18 males and 20 females,were enrolled.After conventional therapy,all the patients received CBP.Biochemistry,blood gas analysis,oxygenation index,mean arterial blood pressure(MAP),acute physiology and chronic health evaluation(APACHE) II scores were monitored.RESULTS:After CBP,the vital signs of patients were rapidly stable,and electrolyte disorders and acid-base imbalance were corrected.Renal function,blood gas,oxygenation index were all improved.MAP was increased,and APACHE II score was significantly decreased.All patients had good tolerance,stable hemodynamics,and no obvious adverse reaction on CBP compared with pre-CBP.CONCLUSION:CBP can effectively clean toxins,correct electrolyte acid-base balance,and improve systemic inflammatory response syndrome and the organ function of MODS patients.

Animal model of non-bacterial multiple organ dysfunction syndrome in the elderly

Objective To establish a model of multiple organ dysfunction syndrome in the elderly (MODSE) by intraperitoneal injection of different doses of zymosan, and to compare the multiple organ dysfunction syndrome (MODS) in adult and in the elderly rats. Methods Adult and senile rats, injected with different doses of zymosan intraperitoneally were examined for the changes in the function and morphology of the vital organs, including heart, liver, brain, lungs, and kidneys using blood gas and biochemistry analysis and histopathological examination methods. Results Compared with the normal controls of the adult and the elderly rats, the blood gas and blood biochemistry changed in different degrees in the different dosed zymosan groups. Pathological changes were also found in the vital organs including lungs, heart, liver, brain, kidneys, erc in the experimental groups. Under the same concentrations of zymosan, the reductions in respiratory, cardiac and renal functions in the senile groups were much more severe than those in the corresponding adult group. In the similar degree of model duplication, the senile rats had the tendency to die later than the adult rats. Conclusions Zymosan can be used in both elderly and adult rats to induce MODS model, and the best dosage for MODSE was 0.Sg/kg injected peritoneally. The model would hopefully be used in the study of mechanisms and the therapeutics on MODSE.

Blood hemoperfusion with resin adsorption combined continuous veno-venous hemofiltration for patients with multiple organ dysfunction syndrome

BACKGROUND:Blood hemoperfusion with resin adsorption can clean larger molecules that exceed the molecular weight cutoff of combined continuous veno-venous hemofiltration(CWH).Hence blood hemoperfusion with resin adsorption combined CWH(HP+CWH) has higher ability of mediator clearance,and can improve clinical outcomes in theory.This study aimed to investigate the effect of blood hemoperfusion with resin adsorption combined continuous veno-venous hemofiltration(HP+CWH) on plasm cytokines like TNF-α,IL-1β,IL-6,cellular immunity and prognosis in patients with multiple organ dysfunction syndrome(MODS).METHODS:This was a prospective,randomized clinical trial.A total of 30 patients who had been diagnosed with MODS were enrolled in this study.Patients were randomly allocated to routine treatment+HP+CWH group(treatment group) and routine treatment+only CWH group(control group).In the treatment group,patients received blood hemoperfusion with resin adsorption for 2hours,and then received CWH for 10 hours every day.In the control group,patients received CWH for 12 hours only every day.The patients in the two groups received blood purification therapy for three days.The plasma of patients in the treatment group was obtained at 0,2,12,24,26,36,48,50,60 hours,5th day,7th day and 10 th day,respectively.The plasma of patients in the control group was obtained at 0,12,24,36,48,60 hours,5th day,7th day and 10 th day,respectively.APACHE Ⅱscore,T-lymphocytes subpopulations,blood lactate acid concentration,heart rate,breathing rate,and oxygenation index were observed.RESULTS:Plasma cytokines like TNF-α,IL-1β,IL-6 decreased markedly after HP(P<0.01);T-lymphocytes subpopulations CD3+,CD4+,CD8+,CD4+/CD8+ increased after HP+CWH or only CWH.The plasma concentrations of TNF-α,IL-1β and IL-6 in the two groups were not markedly different at 12,36,and 50 hours.But on the 5th day,the plasma concentrations of TNF-α,IL-1β and IL-6 in the treatment group were lower than those in the control group(P<0.05).On the 28 th day,5patients died in the treatment group,and 6 patients in the control group.CONCLUSIONS:Both HP+CWH and CWH can clean plasma cytokines like TNF-α,IL-1β,and IL-6,and improve cellular immunity and clinical symptoms and signs of patients.Compared with only CWH,the plasma concentrations of TNF-α,IL-1β and IL-6 were lower on the 5th day,and patients have an increased survival rate on the 28 day in the HP+CWH group.

Magnetic resonance imaging correlates of bee sting induced multiple organ dysfunction syndrome: A case report

Occasionally systemic complications with high risk of death,such as multiple organ dysfunction syndrome(MODS),can occur following multiple bee stings.This case study reports a patient who presented with MODS,i.e.,acute kidney injury,hepatic and cardiac dysfunc-tion,after multiple bee stings.The standard clinical findings were then correlated with magnetic resonance imaging(MRI)findings,which demonstrates that MRI may be utilized as a simpler tool to use than other mul-tiple diagnostics.

Effect of intestinal function-recovering decoction on treatment of multiple organ dysfunction syndrome in rats

Objective:To analyze the effect of intestinal function-recovering decoction on multiple organ dysfunction syndrome in rats,and to investigate a novel solution to multiple organ dysfunction syndrome.Methods:Multiple organ dysfunction syndrome was induced in 60 Sprague-Dawley rats by intestinal ischemia-reperfusion combined with cecal ligation and puncture.Then these rats were intragastrically administered physiological saline(group Ⅰ,n=20),ampicillin(group Ⅱ,n=20) or intestinal function-recovering decoction(group Ⅲ,n=20).After treatment, serum malondialdehyde and superoxide dismutase levels were compared among three groups. Simultaneously,bacterial culture of various organ tissues was performed and bacterial and endotoxin translocation were observed.Results:Compared with group 1,serum malondialdehyde, alanine aminotransferase and aspartate aminotransferase levels were significantly decreased(all P<0.05) and serum superoxide dismutase level was significantly increased(P<0.05) in the group Ⅲ. However,there were no significant differences in these indices between groups Ⅱ and Ⅲ(P>0.05). The rate of bacterial translocation in the groups Ⅱ and Ⅲ was significantly lower than in the group Ⅰ(P<0.05),and no significant difference was observed between groups Ⅱ and Ⅲ(P>0.05). Conclusions:Intestinal function-recovering decoction can significantly reduce endotoxin and bacterial translocation and stabilize enteral oxidative-antioxidative balance.

A Case Report of Scrub Typhus: Secondary Acute Arrest of Hemopoiesis with Multiple Organ Dysfunction Syndromes

Scrub typhus is a zoonotic disease caused by Orientia tsutsugamushi (O. tsutsugamushi) in which humans are accidental hosts. Acute arrest of hemopoiesis (AAH) always manifests in pancytopenia and with supportive treatment or inducement removal, the AAH patients would show significant improvement in blood routine for about a week. As a rapidly progressive and potentially life-threatening organ function disorder syndrome, multiple organ dysfunction syndrome (MODS) is often induced by many factors including infection, illness and injury. We received a rare case of scrub typhus rapidly presenting with AAH and MODS 2 weeks ago. The clinical data of a 32-year-old female with O. tsutsugamushi-induced AAH and MODS was summarized retrospectively and analyzed with a literature review. In this case, we selected tigecycline and moxifloxacin as treatment regimens for scrub typhus. When the potential infection was controlled, her pancytopenia and hepatic function rapidly improved in a few days.

Successful treatment of severe burn patients with multiple organ dysfunction syndrome:A case report

Multiple organ dysfunction syndrome is the presence of altered organ function of two or more organ systems in acute ill patients with severe trauma,burn,shock and infection.In this case,the patient with burn area amounted to 95%and the third-degree burn was up to 90%.He underwent gastrointestinal tract,blood clotting,lung,brain,heart,liver dysfunction,and cardiac arrest for 30 minutes during the courses of treatment,and was discharged from the hospital after 108 days on the basis of comprehensive treatment and repeated skin grafting.

Treatment of a patient with severe lactic acidosis and multiple organ failure due to mitochondrial myopathy:A case report

BACKGROUND Mitochondrial myopathy is a rare genetic disease with maternal inheritance that may involve multiple organ systems.Due to the lack of typical characteristics,its clinical diagnosis is difficult,and it is often misdiagnosed or even missed.CASE SUMMARY The patient was a young college student.When he presented at the hospital,he had severe lactic acidosis,respiratory failure,and shock with multiple organ dysfunction syndrome(MODS).He was treated by mechanical ventilation,venoarterial extracorporeal membrane oxygenation,and other organ support.However,his condition continued to worsen.After a thorough and detailed medical and family history was taken,a mitochondrial crisis was suspected.A muscle biopsy was taken.Further genetic testing confirmed a mitochondrial gene mutation(TRNL13243A>G).The final diagnosis of mitochondrial myopathy was made.Although there is no known specific treatment,intravenous methylprednisone and intravenous immunoglobulin were started.The patient’s shock eventually improved.The further course was complicated by severe infection in multiple sites,severe muscle weakness,and recurrent MODS.After 2 mo of multidisciplinary management and intensive rehabilitation,the patient could walk with assistance 4 mo after admission and walk independently 6 mo after admission.CONCLUSION More attention should be paid to mitochondrial myopathy to avoid missed diagnosis and misdiagnosis.

乌司他丁联合血液净化治疗老年重症急性胰腺炎合并MODS的疗效及对炎症因子的影响

目的探究老年重症急性胰腺炎合并多器官功能障碍综合征(MODS)患者应用乌司他丁联合血液净化治疗的效果。方法将南阳市中心医院2022年6月至2022年12月期间收治的78例老年重症急性胰腺炎合并MODS按随机数字表法分为两组,对照组39例行血液净化治疗,实验组39例行乌司他丁联合血液净化治疗,比较两组患者的治疗有效率、炎症因子水平[降钙素原(PCT)、C反应蛋白(CRP)、白介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)]、脏器功能[肾功能:血肌酐、肾小球滤过率(GFR);肝功能:丙氨酸氨基转移酶(ALT)、碱性磷酸酶(ALP);心功能:肌酸激酶同工酶(CK-MB)]以及发热、腹痛、恶心呕吐缓解时间。结果实验组患者治疗有效率(94.87%)高于对照组(79.49%),差异有统计学意义(P<0.05)。治疗前,两组患者各炎症因子、各脏器功能指标水平相近,治疗后,两组患者各炎症因子、肌酐、ALT、ALP、CK-MB水平均降低,GER数值均升高,其中,相较于对照组,实验组改善显著,差异有统计学意义(P<0.05)。实验组患者各临床症状缓解时间短于对照组,差异有统计学意义(P<0.05)。结论老年重症急性胰腺炎合并MODS患者应用乌司他丁联合血液净化治疗后,治疗有效率提升,炎症因子水平下降,脏器功能改善,临床症状缓解。

血液灌流联合连续性静脉-静脉血液透析治疗马蜂蜇伤并发MODS患者的效果

目的:探讨血液灌流(HP)联合连续性静脉-静脉血液透析(CVVHD)治疗马蜂蜇伤并发多器官功能障碍综合征(MODS)患者的效果。方法:回顾性分析2017年2月—2021年12月遂宁市中心医院收治的107例马蜂蜇伤并发MODS患者的资料,根据治疗方式不同分为A组和B组。B组53例给予血液灌流联合间歇性血液透析治疗(IHD)治疗,A组54例给予HP联合CVVHD治疗。比较两组临床疗效、住院一般情况、肾肝功能、炎症因子水平。结果:治疗结束后,A组总有效率(94.44%)高于B组(81.13%)(P<0.05)。A组住院时间、机械通气时间、血液净化治疗时间及住ICU时间均短于B组(P<0.05)。治疗结束后,两组肝肾功能、炎症因子各指标较治疗前均降低,A组谷草转氨酶(AST)、谷丙转氨酶(ALT)、血尿素氮(BUN)、血肌酐(Scr)、中性粒细胞/淋巴细胞比(NLR)、C反应蛋白(CRP)及血清降钙素原(PCT)水平均低于B组(P<0.05)。结论:HP联合CVVHD治疗马蜂蜇伤并发MODS患者,可加快肝肾功能恢复,降低炎症因子水平,改善住院一般情况,进而提高临床疗效。

Close Correlation between Development of MODS during the Initial 72h of Hospitalization and Hospital Mortality in Severe Fever with Thrombocytopenia Syndrome

An emerging infectious disease was identified as severe fever with thrombocytopenia syn- drome （SFTS） in central China since late March 2009. We found the patients with SFTS had severe clinical symptoms, and progressed rapidly to multiple organ dysfunction syndrome （MODS） with high fatality rate of 25%-30%. The aim of this study was to assess the significance of risk factors predicting the development of MODS and death in SFTS patients. Consecutive SFTS admissions between May 2009 and September 2011 were analyzed for parameters of organ function during hospitalization using Marshall scoring system for MODS, and platelet counts were recorded on admission and at 24, 48, 72 h and one week after admission. We investigated the kinetics of organ failures and analyzed the associa- tion between age, platelet count and development of MODS or death. A total of 92 SFTS patients were enrolled in this study. Among them, 32 patients with dysfunction of over 4 organs were identified, 45% of them died within 72 h, 72% died within 5 days, and 76% died within 7 days after admission. We also found cumulative Marshall score was significantly higher in death patients （11.76＋2.05） than in survival patients （4.22~1.98） （P〈0.001）. In addition, SFTS patients had older age and lower platelet counts in MODS and death groups. Furthermore, we also observed that there was a close correlation between platelet count on admission and Marshall score （P〈0.001）. High Marshall score, advanced age and lower platelet counts were the main risk factors for the development of MODS, and those factors could predict mortality in SFTS patients, suggesting prompt treatment and close monitoring of severe complications, especially MODS, are of great importance in saving patients＇ lives.

Multiple-Organ Extracorporeal Support Therapies in Critically Ill Patients

The critically ill patient is capable of presenting a multiple organ dysfunction syndrome (MODS) caused by different diseases, which can be infectious (sepsis, septic shock) as well as non-infectious (pancreatitis, large surgeries, traumatic injuries, burn patients and brain injuries), this syndrome is characterized by global hemodynamic and organ perfusion alterations accompanied by an uncontrolled and marked inflammatory response unresponsive to pharmacological treatment due to which extracorporeal organ support can be a viable option. Acute renal lesion can occur in up to 60% of patients receiving intensive care, and close to 10% - 20% require renal replacement therapy (RRT) globally this can be provided as peritoneal dialysis (PD) or intermittent hemodialysis (IHD), continuous renal replacement therapy (CRRT), hybrid therapies known as sustained slow efficiency dialysis (SLED), which combines the benefits IHD and CRRT, slow continuous ultrafiltration (SCUF). Extracorporeal membrane oxygenation (ECMO) and extracorporeal elimination of CO2, have been used more frequently lately, these are temporal artificial support used for respiratory and/or cardiac insufficiency that is refractory to conventional treatment. Acute liver failure in adults has a mortality rate close to 50% furthermore one-third of patients hospitalized for cirrhosis are likely to progress to acute liver failure which will drastically increase its mortality. Based on concepts of albumin dialysis, one of its most known is the following: Molecular Adsorbent Recirculating System (MARS), Fractionated Plasma Separation and Absorption—FPSA (Prometheus®) and also, hemoperfusion with different cartridges used in different extracorporeal therapies, used in liver failure, rhabdomyolysis, cytokine release syndrome and more in the context of the pandemic covid19. The objective of this review is to know the different extracorporeal therapies and the therapeutic utility in critical patients.

血液灌流和连续性肾脏替代治疗对百草枯中毒并发MODS患者的应用评价

目的探讨血液灌流(HP)和连续性肾脏替代治疗(CRRT)对百草枯中毒并发多器官功能障碍综合征(MODS)患者的疗效。方法回顾性分析2019年1月至2022年1月诊治的急性百草枯中毒并发MODS患者56例,其中HP治疗者27例,HP+CRRT治疗者29例,比较两组治疗前后肝肾功能、血气分析、MODS评分和APACHE II评分,Kaplan-Meier生存分析比较不同治疗方法的生存率。结果两组治疗前血液生化和血气分析参数、MODS和序贯性器官功能衰竭评分(sequential organ failure assessment,SOFA)比较差异无统计学意义(P>0.05),治疗后两组丙氨酸氨基转移酶(alanine aminotransferase,ALT)、天冬氨酸氨基转移酶(aspartate aminotransferase,AST)、血尿素氮(blood urea nitrogen,BUN)、血肌酐(serum creatinine,Scr)、凝血酶原时间(prothrombin time,PT)、凝血酶原活性(prothrombin activity,PA)、总胆红素(total bilirubin,TBil)、直接胆红素(direct bilirubin,DBil)、肌酸激酶同工酶(creatine kinase isoenzymes,CKMB)、二氧化碳分压(partial pressure of carbon dioxide,PaCO_(2))、肺泡动脉氧分压差[P(A-a)O_(2)]、MODS和SOFA评分均低于治疗前,pH值、氧分压(partial pressure of oxygen,PaO_(2))、碳酸氢根(HCO-3)和氧合指数(PaO_(2)/FiO_(2),P/F)均高于治疗前,且HP+CRRT组改善较HP组更为明显(均P<0.05)。56例患者总死亡率为44.64%,HP组总死亡率高于HP+CRRT组,生存曲线显示HP+CRRT组存活率更高(P<0.05)。结论HP联合CRRT可减轻器官损害,降低百草枯并发MODS患者的死亡率,是一种有效的治疗方法。

血浆置换与血液灌流对蜂蜇伤所致MODS患者疗效对比

目的探讨血浆置换与血液灌流治疗对蜂蜇伤所致多器官功能障碍综合征(MODS)患者疗效的差异。方法分析2015年7月至2022年6月重庆医科大学附属第一医院铜梁医院收治的32例蜂蜇伤所致MODS患者的临床资料,按真实世界研究方法,将其中行连续性静脉-静脉血液滤过(CVVH)联合血液灌流治疗者设为对照组(9例),行CVVH联合血浆置换治疗者设为观察组(23例)。对比分析2组患者预后、住院时间,以及治疗前后部分炎症及生化指标变化,包括C反应蛋白、白细胞、肌酸激酶同工酶等。结果2组患者治疗后C反应蛋白、白细胞、肌酸激酶、肌酸激酶同工酶、乳酸脱氢酶、谷草转氨酶、谷丙转氨酶、肌酐均较治疗前明显降低,且观察组患者治疗后肌酐降低更明显,住院时间更短,差异均有统计学意义(P<0.05)。2组患者预后比较,差异无统计学意义(P>0.05)。结论对蜂蜇伤所致MODS患者给予CVVH联合血浆置换与联合血液灌流治疗均有效,且CVVH联合血浆置换的疗效更显著。

Influence of continuous blood purification on inflammation and target organ damage in patients with severe acute pancreatitis complicated by MODS

Objective:To study the influence of continuous blood purification on inflammation and target organ damage in patients with severe acute pancreatitis accompanied by MODS.Methods: A total of 78 patients with severe acute pancreatitis complicated by MODS who were treated in our hospital between June 2012 and March 2016 were selected and divided into control group (n=39) and observation group (n=39) according to random number table. Control group were treated with routine treatment, observation group were treated with conventional treatment plus continuous blood purification, and serum inflammatory factors, liver function indexes and renal function indexes were compared between two groups of patients before and after treatment.Results: Before treatment, differences in serum levels of inflammatory factors, liver function indexes and renal function indexes were not statistically significant between two groups of patients. After treatment, serum inflammatory factors IL-6, IL-8, MCP-1 and HMGB1 levels of observation group were lower than those of control group, liver function indexes ALT, AST, TBIL and ALP levels of observation group were lower than those of control group, and renal function indexes Scr and BUN levels of observation group were lower than those of control group.Conclusion: Continuous blood purification can reduce the systemic inflammatory response as well as liver and kidney injury in patients with severe acute pancreatitis complicated by MODS.

短肽型营养制剂治疗重症急性胰腺炎的效果及对ARDS、MODS发生率的影响

目的探讨短肽型营养制剂治疗重症急性胰腺炎(SAP)的效果及对急性呼吸窘迫综合征(ARDS)、多器官功能障碍(MODS)发生率的影响。方法选取2019年7月至2020年9月我院收治的60例SAP患者作为研究对象,根据治疗方法将其分为对照组和观察组,各30例。两组患者均接受常规治疗,对照组在此基础上加肠外营养支持治疗,观察组在对照组的基础上加短肽型营养制剂肠内营养支持治疗。比较两组的治疗效果。结果治疗后,观察组的血红蛋白(Hb)、总蛋白(TP)、白蛋白(ALB)、前白蛋白(PA)水平高于对照组(P<0.05)。治疗后,观察组的内毒素、D-乳酸、二胺氧化酶、血淀粉酶水平低于对照组(P<0.05)。治疗后,观察组的C-反应蛋白(CRP)、高迁移率族蛋白B1(HMGB1)、热休克蛋白70(HSP-70)、人可溶性细胞间黏附分子-1(sICAM-1)水平低于对照组(P<0.05)。观察组的ARDS、MODS发生率低于对照组(P<0.05)。结论短肽型营养制剂治疗SAP的效果显著,能够改善患者的肠黏膜功能,抑制炎症因子表达,降低ARDS、MODS发生率,提高患者的生活质量。

Mutating Escherichia coli caused multiple organ dysfunction syndrome

Pathogenic Escherichia coli is of great concern in the clinical setting.But few reports have demonstrated the variation in disease course.We present a severe case of multiple organ dysfunction syndrome caused by E.coli infection.Pathogens isolated from blood and urine samples harboured many virulence factors.Whole‐genome sequencing and conventional analyses showed that the isolates experienced beneficial variations,both genetically and phenotypically,during the disease course.These findings showed that E.coli can cause sys-temic symptoms and informed us of the importance of assessing the reasons for such variations in pathogens occurring in vivo.

外周血受体相互作用蛋白激酶3、混合系列蛋白激酶样结构域水平与新生儿坏死性小肠结肠炎病情严重程度的关系

目的分析新生儿坏死性小肠结肠炎(NEC)患儿外周血受体相互作用蛋白激酶3(RIPK3)、混合系列蛋白激酶样结构域(MLKL)的表达情况及其与病情严重程度的关系。方法选取92例NEC患儿纳入NEC组,并根据病情严重程度进一步分为轻度NEC组(Ⅰ级)60例和重度NEC组(Ⅱ~Ⅲ级)32例,另选取同期诊治的60例腹股沟斜疝患儿纳入对照组。采用实时荧光定量聚合酶链反应检测外周血RIPK3 mRNA、MLKL mRNA表达;采用Pearson相关分析法明确NEC组外周血RIPK3 mRNA与MLKL mRNA表达的相关性;采用免疫印迹法检测NEC回肠组织和正常回肠组织中RIPK3、MLKL蛋白表达;采用多因素Logistic回归分析明确重度NEC发生的独立危险因素。绘制受试者工作特征曲线,分析外周血RIPK3 mRNA、MLKL mRNA单独及联合预测重度NEC的价值。结果NEC组外周血RIPK3 mRNA、MLKL mRNA相对表达量分别为(2.41±0.52)、(3.03±0.64),高于对照组的(1.02±0.21)、(0.93±0.20),差异有统计学意义(P<0.001)。NEC回肠组织中RIPK3、MLKL蛋白相对灰度值分别为(1.20±0.21)、(1.13±0.24),高于正常回肠组织的(0.34±0.12)、(0.32±0.11),差异有统计学意义(P<0.05)。NEC组患儿外周血RIPK3 mRNA与MLKL mRNA相对表达量呈正相关(r=0.623,P<0.001)。重度NEC组合并气腹征、多器官功能障碍综合征、败血症者占比和RIPK3 mRNA、MLKL mRNA相对表达量均高于轻度NEC组,差异有统计学意义(P<0.05);RIPK3 mRNA、MLKL mRNA相对表达量升高是重度NEC发生的独立危险因素(P<0.05)。外周血RIPK3 mRNA、MLKL mRNA联合预测重度NEC的曲线下面积大于RIPK3 mRNA、MLKL mRNA单独预测(Z=4.127、4.261,P<0.05)。结论RIPK3 mRNA、MLKL mRNA在NEC患儿外周血中表达升高,两者均与NEC病情严重程度有关,且两者联合检测对重度NEC具有较高的预测价值。