Integration of network pharmacology and bone marrow mesenchymal stem cells experimental research to reveal the molecular mechanisms for Hai Honghua medicinal liquor against osteoporosis

Background:Hai Honghua medicinal liquor(HHML)formula has been used in clinical practice for a long time,mainly for the treatment of freshly closed fractures,to promote osteogenesis,to increase bone mass,and thus to promote fracture healing.However,the underlying mechanisms of HHML in the treatment of osteoporosis(OP)are still unclear.Methods:Firstly,Traditional Chinese Medicines Systems Pharmacology Database and Analysis Platform and The Encyclopedia of Traditional Chinese Medicine were used to screen the targets of the active compounds of HHML.At the same time,OP targets were identified through GeneCards,Online Mendelian Inheritance in Man,DisGeNET,Therapeutic Target Database,Comparative Toxicogenomics Database and Human Phenotype Ontology databases.Next,protein-protein interaction and pathway networks were constructed for compound-disease common targets,and core targets and compounds were screened for molecular docking.Furthermore,rat bone marrow mesenchymal stem cells were extracted as model cells,and the osteogenic effects of HHML were verified via in vitro experiments.Results:Total of 343 common targets of HHML-OP and the top 10 target proteins in the protein-protein interaction network are TP53,AKT1,STAT3,HSP90AA1,ESR1,TNF,IL6,MAPK1,MAPK3 and MAPK8.Enrichment analysis yielded that the key molecular pathway was the PI3K/Akt signaling pathway.Molecular docking analysis showed that baicalein,erysodienone and naringenin docked with the target proteins AKT1,STAT3 and TP53,respectively,with low binding energy and high affinity.In addition,In vitro experiments demonstrated that HHML promoted the proliferation of bone marrow mesenchymal stem cells;compared with the control group,HHML-treated cells showed enhanced alkaline phosphatase staining,promoted the expression of OCN,RUNX2,BSP,and COL1 mRNAs as well as the expression of PI3K and AKT phosphorylated proteins.Secondly,the expression of target proteins revealed that HHML promoted the phosphorylation of STAT3 protein and inhibited the expression of P53.Conclusions:Our study investigated that HHML treatment with OP promotes bone formation possibly through activation of the PI3K/Akt signaling pathway and may involve STAT3 and TP53 target interactions.

The mechanistic study on Wa medicine Niang-Mu-Liang medicinal liquor mitigating diabetes mellitus erectile dysfunction in rats by inhibiting ferroptosis

Background:This study aims to investigate the therapeutic effect of Wa medicine Niang-Mu-Liang medicinal liquor(NML)on rats with diabetes mellitus erectile dysfunction(DMED)and its impact on the ferroptosis signaling pathway.Methods:Thirty Sprague-Dawley rats were randomly divided into three groups:Control,DMED,and NML.After establishing the DMED model,treatments were administered for 8 weeks.After the administration,apomorphine hydrochloride tests were conducted to measure the mass and organ index of testes and epididymides,sperm concentration and viability in each group.Penile corpus cavernosum tissues were stained with hematoxylin and eosin.Nitric oxide and cyclic guanosine monophosphate levels in the penile corpus cavernosum tissues were determined using biochemical kits and enzyme-linked immunosorbent assay,while the expression of proteins related to the ferroptosis signaling pathway was measured by Western blot.Results:Compared to the DMED group,the DMED rats treated with NML showed significantly increased erection frequency,testicular and epididymal mass and index,sperm count and viability,along with noticeable improvement in the pathological morphology of penile corpus cavernosum.The content of nitric oxide and cyclic guanosine monophosphate,and the expression of ferritin heavy chain,ferritin light chain,and glutathione peroxidase 4 proteins in penile corpus cavernosum tissue were elevated,while the expression of transferrin and STEAP3 proteins was reduced.Conclusion:NML can improve erectile function in DMED rats by inhibiting the ferroptosis signaling pathway.

Relationship between Al(OH)_3 solubility and particle size in synthetic Bayer liquors

Surface tension of sodium aluminate solution and the contact angle between Al（OH）3 particles and aluminate solution were measured, then the dependence of Al（OH）3 solubility on its particle size was calculated and thus the variation of the critical nucleus sizes was determined based on the Ostwald ripening formula. The results show that the Al（OH）3 solubility in sodium aluminate solution decreases with the increment of particle size, and the critical nucleus sizes increase with the rise of alkali concentration, caustic ratio and precipitation temperature. The results also imply that the presence of small particles in seeded precipitation system is an important factor to limit the depth of precipitation.

固相法合成钴掺杂锰系锂离子筛的吸附性能

随着5G/6G通信、新能源汽车、锂电池产业的快速发展,近年来对锂化合物(尤其是Li_(2)CO_(3))的需求急剧增加,许多国家将锂视为战略矿产资源.目前从锂沉淀母液中提取锂受到了极大的关注.锰系离子筛(LMO)是一种具有广泛应用前景的吸附剂,可实现复杂溶液中锂的高效回收.但LMO在酸洗解吸过程中存在锰溶损严重的难题,会降低吸附性能和循环性能.针对上述问题,本研究提出引入Co^(3+)掺杂降低LMO锰溶解损失的策略,制备了钴掺杂锰系离子筛(LCMO).采用X射线衍射(XRD)、扫描电子显微镜(SEM)、透射电子显微镜(TEM)、X射线光电子能谱(XPS)等方法对不同煅烧温度和焙烧时间下制备的LCMO进行表征分析.表征结果表明,Co掺杂对LMO的尖晶石结构没有影响,并且Co最佳掺杂摩尔分数为5%,此时离子筛前驱体中Mn^(3+)的原子分数从未掺杂的9.67%降低到3.63%,对应的锂吸附容量从39.299 mg·g^(-1)显著增加到41.708 mg·g^(-1),锰溶损也从1.288%显著降低至0.837%,大大地增加了锰系离子筛的实际应用可能性.制备的摩尔分数为5%Co掺杂的离子筛(LCMO-5%)具有良好的循环性能,Li^(+)的吸附能力在5次循环后仍然保持在81%以上.在模拟的锂沉淀母液中,Li/Na和Li/K之间的分离系数分别为74.655和64.547,这证明了LCMO-5%能有效地从高Na^(+)、K^(+)溶液中吸附分离Li^(+).因此,LCMO-5%离子筛具有从锂沉淀母液中提取Li+的应用前景.

A win-win technique of stabilizing sand dune and purifying paper mill black-liquor

The principle and technique were reported here to produce lignin-based sand stabilizing material （LSSM） using extracted lignin from black liquor of straw paper mills. Field tests using LSSM to stabilize and green sand dunes were started in 2002. The field experiment was carded out in August 2005 when the newly formed plant community was 3 years old. The results from the comprehensive field experiment demonstrated that unlike polyvinyl acetate or foamed asphalt commonly used for dune stabilization, LSSM was plant- friendly material and could be used in combination with seeding and planting of desert species. With the help of LSSM, the desert species （i.e., Agriophyllum squarrosum （L.） Moq. and Artemisia desertorum Spreng. etc.） could be used to form community in 2-3 yeas and to stabilize sand dune effectively. The newly formed community was sustainable under an extremely dry climate condition. The organic matter and total nitrogen in the soil increased significantly as the community were formed, while the change in P and K contents in the soil was negligible.

Treatment of black liquor from the papermaking industry by acidification and reuse

Two different kinds of black liquor from the papermaking industry were treated by acidification and reuse. The experimental parameters and conditions were discussed in detail. The experimental results indicated that the treatment process mentioned in this article is an effective process for the treatment of black liquor from the papermaking industry. By the treatment, the solid materials in black liquor are transferred into two by products and the other components are reused or evaporated. Thus, no wastewater except some condensation water would be discharged in pulping process and the problem of pollution of black liquor would be effectively solved.

Effect of Maotai liquor on the liver: an experimental study

BACKGROUND: Epidemiology investigation showed that no worker drunk Maotai liquor for nearly 30 years died of hepatic diseases, and no obvious hepatic fibrosis and cirrho- sis were found in 99 workers who had drunk Maotai liquor for a long period by epidemiology investigation and needle biopsy. The same finding was detected in rats that were drunk by Maotai liquor continued for 56 days. This study was to investigate the effects of Maotai liquor on the liver and its mechanism of preventing hepatic fibrosis. METHODS: After ingestion of Maotai for 56 consecutive days, male SD rats were killed for detecting the levels of metallothionein and malondialdehyde (MDA) in liver tis- sues. Rat hepatic stellate cells (HSCs) and human HSCs were cultured in vitro to observe the effect of Maotai on HSCs proliferation and collagen synthesis. After ingestion of Maotai for 14 consecutive weeks, the livers of male SD rats were harvested for pathohistological examination. RESULTS: The level of metallothionein in the liver of Mao- tai-induced rats increased by 22 folds, whereas the levels of hepatic lipid peroxide and MDA was decreased significantly ( P <0.05) in Maotai-induced animals suffering from CCl4. Maotai demonstrated obvious inhibitory effect on prolifera- tion of HSCs and the inhibition was concentration-depen- dent. Gene expression and protein secretion of collagens could also be inhibited by Maotai. In alcoholic group, typi- cal liver cirrhosis was observed. In Maotai group, however, though fatty degeneration of hepatocytes and mild fibrosis of the interstitium were observed, no obvious hepatic fibro- sis and cirrhosis were found. CONCLUSION: It might be an important mechanism of interfering the progress of hepatic fibrosis that Maotai in- creases the level of metallothionein in the liver and inhibits the activation of HSCs and the synthesis of collagen pro- teins.

Comparison of Algorithms for an Electronic Nose in Identifying Liquors

When the electronic nose is used to identify different varieties of distilled liquors, the pattern recognition algorithm is chosen on the basis of the experience, which lacks the guiding principle. In this research, the different brands of distilled spirits were identified using the pattern recognition algorithms （principal component analysis and the artificial neural network）. The recognition rates of different algorithms were compared. The recognition rate of the Back Propagation Neural Network （BPNN） is the highest. Owing to the slow convergence speed of the BPNN, it tends easily to get into a local minimum. A chaotic BPNN was tried in order to overcome the disadvantage of the BPNN. The convergence speed of the chaotic BPNN is 75.5 times faster than that of the BPNN.

Combined effect of US/PFS on the black liquor of making paper

The reed pulping black liquor is used as a sample. Researches of (1) combined effect of ultrasound(US)/polyferric sulphate(PFS)/hydrogen peroxide(H 2O 2); (2) effects of the horn tip areas; (3) effect of dose of PFS were carried. Extrapolation of these experiments indicated treatment results are possibly related to complex chemical compositions in the reed pulping black liquor.

Application of Two-Dimensional Correlation UV-Vis Spectroscopy in Chinese Liquor Moutai Discrimination

Chinese liquor Moutai is the “National alcoholic drink” in China and plays a very important role of social activities in Chinese people’s life. In pursuit of high profits, some illegal counterfeit Moutai liquors have begun to appear in the market. Therefore, it is an urgent need for new techniques to discriminate the genuine and counterfeit Moutai liquor. In this work, the conventional Ultraviolet-Visible (UV-Vis) spectroscopy and two-dimensional correlation UV-Vis spectroscopy are applied to obtain the UV-Vis characteristic of Moutai liquor and counterfeit one, respectively. The experimental results reveal that the conventional UV-Vis spectra of the genuine and counterfeit Moutai liquor are similar. However, the two-dimensional correlation UV-Vis spectra of them are different and this method would be applied to differentiate the counterfeit Moutai liquor from the genuine Moutai liquor. Compared with conventional methods, this novel method has the advantages of easy operation, simple instrumentation and direct recognition, which make it a potential tool in the fields of food safety.

Hydraulic and carbonizing actions of sulfuric acid to straw pulp and paper black liquor

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Idiopathic mesenteric phlebosclerosis associated with long-term use of medical liquor:Two case reports and literature review

A 62-year-old woman was admitted to our hospital in2011 because of recurrent abdominal pain,nausea and constipation for six months.Computed tomography enterography(CTE)showed tortuous thread-like calcifications in the ileocolic vein and right colic vein,while colonoscopy revealed purple-blue mucosa extending from the cecum to the splenic flexure.Based on the results of these tests,the patient was diagnosed with idiopathic mesenteric phlebosclerosis(IMP).She had a history of Chinese medical liquor intake for one and a half years and her symptoms subsided after conservative treatment.In 2013,a 63-year-old male patient who presented with recurrent lower right abdominal pain,bloating,melena and diarrhea for fifteen months was admitted to our institution.Colonoscopy and CTE led to the diagnosis of IMP.He also used Chinese medical liquor for approximately 12 years.The patient underwent total colectomy and the postoperative course was uneventful.We searched for previously published reports on similar cases and analyzed the clinical data of 50 cases identified in PubMed.As some of these patients admitted use of Chinese medicines,we hypothesize that Chinese medicines may play a role in the pathogenesis of IMP.

Isolation of Hemicellulose from Wood Chips via Extraction with Kraft Green Liquor

The present paper covers the extraction of hemicelluloses from woods, i.e., Eucalyptus Globulus, white fir and Douglas fir via green liquor pretreatment and alkali post-treatment with sodium hydroxide. The effects of such extraction conditions as extraction time, temperature on the yields of hemicellulose and the degree of deliginification were investigated. Sugar analyses using Dionex indicate that xylose, glucose and mannose are the main sugar types present in the hemicellulose from Eucalyptus. Other sugar components such as arabinose and galactose were also found in Dogulas fir hemicellulose. The highest yield of hemicellulose was 8.4% for Eucalyptus extracted with green liquor at 130 ℃ for 3.5 h. Further sodium hydroxide extraction for 5 h gave a hemicellulose yield of about 20%. The use of green liquor for the extraction of wood hemicellulose is believed to be the first attempt.

Catalytic Activity of the Black Liquor and Calcium Mixture in CO2 Gasification of Fujian Anthracite

CO2 gasification of Fuijian high-metamorphous anthracite with black liquor （BL） and/or mixture of BL and calcium stuff （BL＋Ca） as catalyst was studied by using a thermogravimetry under 750-950℃ at ambient pressure. When the coal was impregnated with an appropriate quantity of Ca and BL mixture, the catalytic activity of CO2 gasification was enhanced obviously. With a loading of 8%Na-BL＋2%Ca, the carbon conversion of three coal samples tested reaches up to 92.9%-99.3% at 950℃ within 30min. The continuous formation of alkali surface compounds such as （[-COM], [-CO2M]） and the presence of exchanged Ca, such as calcium phenolate and calcium carboxylates （COO）2Ca, contribute to the increase in catalytic efficiency, and using BL＋Ca is more efficient than that adding BL only, The homogeneous model and shrinking-core model were applied to correlate the data of conversion with time and to estimate the reaction rate constants under different temperature. The corresponding reaction activation energy （Ea） and pre-exponential factor of three anthracites were estimated. It is found that Ea is in the range from 73.6 to 121.4kJ·mol^-1 in the case of BL＋Ca, and 74.3 to 104.2kJ·mol^-1 when only BL was used as the catalyst, both of which are much less than that from 143.5 to 181.4kJ·mol^-1 if no catalyst used. It is clearly demonstrated that both of BL＋Ca mixture and BL could be the source of cheap and effective catalyst for coal gasification.

Investigation on Steam Gasification of High-metamorphous Anthracite Using Mixed Black Liquor and Calcium Catalyst

The catalytic effects of single and mixed catalysts, i.e. single 3%Ca and 5%Na-BL（black liquor） catalysts and mixed 3%Ca＋5%Na-BL catalyst, on carbon conversion, gasification reaction rate constant and activation energy, relative amount of harmful pollutant like sulphur containing gases have been investigated by thermogravimetry in steam gasification under temperature 750℃ to 950℃ at ambient pressure for three high-metarnorphous anthracites （Longyan, Fenghai and Youxia coals in Fujian Province）. The mixed catalyst of 3%Ca＋5%Na-BL increases greatly the carbon conversion and gasification rate constant by accelerating the gasification reaction C＋H2O→CO＋H2 due to presence of alkali surfacecompounds [COM], [CO2M] and exchanged calcium phenolate and calcium carboxylate （-COO）2. By adding CaCO3 into BL catalyst in gasification, in addition to improving the catalyst function and enhancing the carbon conversion, the effective desulphurization is also achieved, but the better operating temperature should be below 900℃. The homogenous and shrinking core models can be successfully employed to correlate the relations between the conversion and the gasification .time .and to estimate the reaction rate constant, The reaction acUvaUon energy and pre-exponential factor are estimated and the activation energy for mixed catalyst is in a range of 98.72-166.92 kJ·mol^-1, much less than 177.50-196.46 kJ·mol^-1 for non-catalytic steam gasification for three experimental coals.

Acid precipitation coupled electrodialysis to improve separation of chloride and organics in pulping crystallization mother liquor

Inefficient separation of inorganic salts and organic matters in crystallization mother liquor is still a problem to industrial wa stewater treatment since the high salinity significantly impedes organic pollutant degradation by oxidation or incineration.In the study,acidification combined electrodialysis(ED)was attempted to effectively separate Cl-ions from organics in concentrate pulping wastewater.Membrane’s rejection rate to total organic carbon(TOC)was 85%at wastewater intrinsic pH=9.8 and enhanced to 93%by acidifying it to pH=2 in ED process.Negative-charged alkaline organic compounds(mainly lignin)could be liberated from their sodium salt forms and coagulated in acidification pretreatment.Neutralization of the organic substances also made their electro-migration less effective under electric driving force and in particular improved separation efficiency of chloride and organics.After acid-ED coupled treatment(pH=2 and J=40 mA·cm-2)[TOC]remarkably reduced from 1.315 g·L-1 to 0.048 g·L-1 and[Cl-]accumulated to 130 g·L-1 in concentrate solution.Recovery rate of NaCl was 89%and the power consumption was 0.38 kW·h·kg-1 NaCl.Irreversible fouling was not caused as electric resistance of membrane pile maintained stably.In conclusion,acidic-ED is a practical option to treat salinity organic wastewater when current techniques including thermal evaporation and pressure-driven membrane se paration present limitations.

Solvent extraction of V(Ⅴ) and Cr(Ⅲ) from acidic leach liquors of ilmenite using Aliquat 336

Extraction of V（V） and Cr（III） from acidic sulfate leach liquors of ilmenite using 0.4 mol/L Aliquat 336 chloride in kerosene was carried out. Different parameters affecting the extraction process such as equilibrium time, sulfate concentration, Aliquat 336 concentration, equilibrium p H and the extraction temperature were investigated. Extraction of V（V） and Cr（III） by Aliquat 336 involved anion exchange mechanism, and the extracted species are [（VO2SO4）R4N]org at low equilibrium p H for V（V） and [R4N-Cr（OH）4]org at high equilibrium p H for Cr（III）. Calculated thermodynamic parameters show that the extraction process is endothermic reaction for V（V） and exothermic for Cr（III）. Also, calculated values of ？Gex and ？Sex indicate that the extraction reactions of V（V） and Cr（III） proceed as non-spontaneous reaction is more random. V（V） and Cr（III） were stripped, precipitated, separated and calcined at 500 °C for 2.0 h to produce the corresponding oxide in pure form after rinsing and drying.

Near-Infrared Spectroscopy Combined with Partial Least Squares Discriminant Analysis Applied to Identification of Liquor Brands

The identification of liquor brands is very important for food safety. Most of the fake liquors are usually made into the products with the same flavor and alcohol content as regular brand, so the identification for the liquor brands with the same flavor and the same alcohol content is essential. However, it is also difficult because the components of such liquor samples are very similar. Near-infrared (NIR) spectroscopy combined with partial least squares discriminant analysis (PLS-DA) was applied to identification of liquor brands with the same flavor and alcohol content. A total of 160 samples of Luzhou Laojiao liquor and 200 samples of non-Luzhou Laojiao liquor with the same flavor and alcohol content were used for identification. Samples of each type were randomly divided into the modeling and validation sets. The modeling samples were further divided into calibration and prediction sets using the Kennard-Stone algorithm to achieve uniformity and representativeness. In the modeling and validation processes based on PLS-DA method, the recognition rates of samples achieved 99.1% and 98.7%, respectively. The results show high prediction performance for the identification of liquor brands, and were obviously better than those obtained from the principal component linear discriminant analysis method. NIR spectroscopy combined with the PLS-DA method provides a quick and effective means of the discriminant analysis of liquor brands, and is also a promising tool for large-scale inspection of liquor food safety.

Growth properties of mixed liquor suspended solids in SMBR for pharmaceutical wastewater treatment

This paper presents the performance results of a 366-day pilot-scale submerged membrane bioreactor (SMBR) for treating high-strength pharmaceutical wastewater. The study focuses on the growth properties of mixed liquor suspended solids (MLSS) in SMBR operated at high volumetric loading rates. The influences of MLSS on COD removal,sludge yield,oxygen utilization rates and sludge viscosity are studied. Results show that the bioreactor can be operated at higher volumetric loading rate with a low sludge yield. VSS/SS and observed sludge yield coefficient (Yo) present a decreasing trend with the decrease of hydraulic retention time (HRT) . Sludge oxygen utilization rate decreases with the increase of mixed liquor volatile suspended solids (MLVSS) . A mathematical model between sludge viscosity and MLSS is developed.

Synthesis of Fe_3O_4 Magnetic Powder from Spent Pickling Liquors

Spent pickling liquors pose a serious environmental problem in most industrialized countries, mainly owing to their corrosive properties and their ferrous iron and hydrochloric acid content. In this paper, spent pickling liquor was used as an inexpensive raw material to prepare Fe304 magnetic powder via an oxidation method. Being able to recover the dissolved iron from spent pickling liquors would not only salvage a valuable material but also render the effluent environmentally benign. The structure of the Fe_3O_4 magnetic powder was characterized by X-ray diffraction. The morphology and size were characterized by scanning electron microscopy and transmission electron microscopy. Their magnetic properties were tested at room temperature by a vibrating sample magnetometer. In addition, the saturation magnetization of Fe_3 O_4 products can be further enhanced to 96.1 emu/g after purification.