[Objectives] This study was conducted to establish a method for determination of chlorogenic acid in Mussaenda pubescens Ait.f.[Methods]HPLC used SHISEIDO C_(18) MG Ⅱ column( 5 μm,4.6 mml.D.× 250 mm) as chromat...[Objectives] This study was conducted to establish a method for determination of chlorogenic acid in Mussaenda pubescens Ait.f.[Methods]HPLC used SHISEIDO C_(18) MG Ⅱ column( 5 μm,4.6 mml.D.× 250 mm) as chromatographic column and acetonitrile-0.4% phosphoric acid solution( 9∶91) as mobile phase.The separation was performed at a flow rate of 1.0 ml/min and a column temperature of 30 ℃,and the detection wavelength was set at 327 nm.[Results]Chlorogenic acid had a good linear relation in the range of 0.254 7-2.547 5 μg( R2= 0.999 9).The recovery rate was 97.8%,RSD = 1.82%( n = 9).[Conclusions]The content of chlorogenic acid in M.pubescens was determined by ultrasonic extraction and HPLC.The method was simple,stable and reliable and could be used for the quality control of M.pubescens.展开更多
Physalis pubescens L.(P.pubescens)was widely used for the treatment of inflammation-related diseases,such as sore throat,aphonia,phlegm,heat,and cough in folk medicine.The fruits of P.pubescens are commonly consumed a...Physalis pubescens L.(P.pubescens)was widely used for the treatment of inflammation-related diseases,such as sore throat,aphonia,phlegm,heat,and cough in folk medicine.The fruits of P.pubescens are commonly consumed as fruit in many areas of the world.In the past few decades,the phytochemistry and pharmacology of P.pubescens were extensively investigated.About 170 chemical constituents were purified from P.pubescens.The extract and chemical constituents of P.pubescens demonstrate diverse pharmacological effects,including anti-oxidation,anti-inflammation,anticancer,antimicrobial activity,immunomodulation,diuretic effect,hypoglycemic,and hypolipidemic in vitro and in vivo.Herein,we systematically summarized the ethnomedicinal uses,botanical characterization,distribution,phytochemistry,and pharmacology of P.pubescens,and establish the correlation between chemical constituents and pharmacological effects.展开更多
Sigesbeckiae Herba,a dry whole plant of Sigesbeckia glabrescens Makino,Sigesbeckia pubescens Makino and Sigesbeckia orientalis L.,is a traditional Chinese medicine used to dispel dampness,dredge meridians and collater...Sigesbeckiae Herba,a dry whole plant of Sigesbeckia glabrescens Makino,Sigesbeckia pubescens Makino and Sigesbeckia orientalis L.,is a traditional Chinese medicine used to dispel dampness,dredge meridians and collaterals,clear heat and detoxicate.Its chemical components are complex and abundant,including mainly diterpenoids,sesquiterpenoids and flavonoids.Among them,diterpenoids are the main active components.Modern pharmacological studies have found that the compounds isolated from Sigesbeckiae Herba have anti-inflammatory,anti-tumor,antithrombus and other pharmacological activities.This review systematically summarized the chemical components identified from Sigesbeckiae Herba and their pharmacological activities by searching Chinese and English databases,in order to provide references for the further development of Sigesbeckiae Herba and the improvement of its quality standards.展开更多
目的：建立玉叶金花总三萜类成分含量的测定方法。方法：以熊果酸作对照品,采用L9（34）正交设计优选显色条件,并测定玉叶金花药材中总三萜类成分的含量。结果：最佳显色条件为：5%香草醛-冰醋酸溶液0.6 m L,高氯酸0.6 m L,70℃,反应15 ...目的：建立玉叶金花总三萜类成分含量的测定方法。方法：以熊果酸作对照品,采用L9（34）正交设计优选显色条件,并测定玉叶金花药材中总三萜类成分的含量。结果：最佳显色条件为：5%香草醛-冰醋酸溶液0.6 m L,高氯酸0.6 m L,70℃,反应15 min;该方法在0.021-0.16 mg含量范围内线性关系良好（R=0.9995）,当显色反应完全后对照品和供试品液在70 min内稳定性良好,精密度高（RSD=2.00%,n=5）,平均回收率为100.08%（RSD=1.18%,n=6）。结论：香草醛比色法适合玉叶金花总三萜类成分的含量测定,可用于其药材的质量控制。展开更多
探讨建立分光光度法测定毛酸浆中的总黄酮含量的方法,通过考察显色剂用量以及加入显色剂后放置时间对毛酸浆总黄酮测定的影响,采用单因素和正交试验优化硝酸铝-亚硝酸钠比色法测定毛酸浆果中总黄酮的含量。结果表明,毛酸浆中总黄酮含量...探讨建立分光光度法测定毛酸浆中的总黄酮含量的方法,通过考察显色剂用量以及加入显色剂后放置时间对毛酸浆总黄酮测定的影响,采用单因素和正交试验优化硝酸铝-亚硝酸钠比色法测定毛酸浆果中总黄酮的含量。结果表明,毛酸浆中总黄酮含量测定的最佳的条件为：5%Na NO2溶液0.8 m L,混匀放置2 min后,加10%Al（NO3）3溶液0.8 m L,混匀,继续加4%Na OH溶液6 m L,定容混匀后立即在335 nm下测其吸光度。在此条件下,芦丁标准溶液在0~0.002 4 mg/m L范围内与吸光度值间的回归方程y=289.02x＋0.008,R2=0.999 5。该测定方法的平均加标回收率为100.83%,稳定性、精密度以及回收率的相对标准偏差（RSD）分别为0.34%,0.29%和2.91%。该方法简便、快速、准确,可以用于毛酸浆果汁中总黄酮含量的测定。展开更多
基金Supported by Guangzi Post-doctor Innovative Practice Base Open Project(2017002A)
文摘[Objectives] This study was conducted to establish a method for determination of chlorogenic acid in Mussaenda pubescens Ait.f.[Methods]HPLC used SHISEIDO C_(18) MG Ⅱ column( 5 μm,4.6 mml.D.× 250 mm) as chromatographic column and acetonitrile-0.4% phosphoric acid solution( 9∶91) as mobile phase.The separation was performed at a flow rate of 1.0 ml/min and a column temperature of 30 ℃,and the detection wavelength was set at 327 nm.[Results]Chlorogenic acid had a good linear relation in the range of 0.254 7-2.547 5 μg( R2= 0.999 9).The recovery rate was 97.8%,RSD = 1.82%( n = 9).[Conclusions]The content of chlorogenic acid in M.pubescens was determined by ultrasonic extraction and HPLC.The method was simple,stable and reliable and could be used for the quality control of M.pubescens.
基金This research was supported by NNSF of China(81673558).
文摘Physalis pubescens L.(P.pubescens)was widely used for the treatment of inflammation-related diseases,such as sore throat,aphonia,phlegm,heat,and cough in folk medicine.The fruits of P.pubescens are commonly consumed as fruit in many areas of the world.In the past few decades,the phytochemistry and pharmacology of P.pubescens were extensively investigated.About 170 chemical constituents were purified from P.pubescens.The extract and chemical constituents of P.pubescens demonstrate diverse pharmacological effects,including anti-oxidation,anti-inflammation,anticancer,antimicrobial activity,immunomodulation,diuretic effect,hypoglycemic,and hypolipidemic in vitro and in vivo.Herein,we systematically summarized the ethnomedicinal uses,botanical characterization,distribution,phytochemistry,and pharmacology of P.pubescens,and establish the correlation between chemical constituents and pharmacological effects.
基金This work was supported by the National Natural Science Foundation of China(No.81903789)the China Postdoctoral Science Foundation(No.2020M670675&No.2020M680988)College Student Innovation and Entrepreneurship Training Program(No.S202010163009).
文摘Sigesbeckiae Herba,a dry whole plant of Sigesbeckia glabrescens Makino,Sigesbeckia pubescens Makino and Sigesbeckia orientalis L.,is a traditional Chinese medicine used to dispel dampness,dredge meridians and collaterals,clear heat and detoxicate.Its chemical components are complex and abundant,including mainly diterpenoids,sesquiterpenoids and flavonoids.Among them,diterpenoids are the main active components.Modern pharmacological studies have found that the compounds isolated from Sigesbeckiae Herba have anti-inflammatory,anti-tumor,antithrombus and other pharmacological activities.This review systematically summarized the chemical components identified from Sigesbeckiae Herba and their pharmacological activities by searching Chinese and English databases,in order to provide references for the further development of Sigesbeckiae Herba and the improvement of its quality standards.
文摘目的：建立玉叶金花总三萜类成分含量的测定方法。方法：以熊果酸作对照品,采用L9（34）正交设计优选显色条件,并测定玉叶金花药材中总三萜类成分的含量。结果：最佳显色条件为：5%香草醛-冰醋酸溶液0.6 m L,高氯酸0.6 m L,70℃,反应15 min;该方法在0.021-0.16 mg含量范围内线性关系良好（R=0.9995）,当显色反应完全后对照品和供试品液在70 min内稳定性良好,精密度高（RSD=2.00%,n=5）,平均回收率为100.08%（RSD=1.18%,n=6）。结论：香草醛比色法适合玉叶金花总三萜类成分的含量测定,可用于其药材的质量控制。
文摘探讨建立分光光度法测定毛酸浆中的总黄酮含量的方法,通过考察显色剂用量以及加入显色剂后放置时间对毛酸浆总黄酮测定的影响,采用单因素和正交试验优化硝酸铝-亚硝酸钠比色法测定毛酸浆果中总黄酮的含量。结果表明,毛酸浆中总黄酮含量测定的最佳的条件为：5%Na NO2溶液0.8 m L,混匀放置2 min后,加10%Al（NO3）3溶液0.8 m L,混匀,继续加4%Na OH溶液6 m L,定容混匀后立即在335 nm下测其吸光度。在此条件下,芦丁标准溶液在0~0.002 4 mg/m L范围内与吸光度值间的回归方程y=289.02x＋0.008,R2=0.999 5。该测定方法的平均加标回收率为100.83%,稳定性、精密度以及回收率的相对标准偏差（RSD）分别为0.34%,0.29%和2.91%。该方法简便、快速、准确,可以用于毛酸浆果汁中总黄酮含量的测定。
