Mycorrhiza helper bacteria(MHB)can promote the formation and functioning of arbuscular mycorrhizal(AM)symbiosis,but their role and application potential in coping with soil-borne diseases are still unclear.A 14-week g...Mycorrhiza helper bacteria(MHB)can promote the formation and functioning of arbuscular mycorrhizal(AM)symbiosis,but their role and application potential in coping with soil-borne diseases are still unclear.A 14-week greenhouse pot experiment was conducted to obtain several actinomycete strains helping AM symbiosis in suppressing the Phytophthora blight of pepper(Capsicum annuum L.),using a soil inoculated with Phytophthora capsici after sterilization.Five Streptomyces strains,including S.pseudogriseolus,S.albogriseolus,S.griseoaurantiacus,S.tricolor,and S.tendae,as well as the AM fungus(Funneliformis caledonium)were tested.The Phytophthora blight severity reached 66%at full productive stage in the uninoculated control,and inoculation of F.caledonium,S.griseoaurantiacus,and S.tricolor alone significantly decreased(P<0.05)it to 47%,40%,and 35%,respectively.Compared to F.caledonium alone,additional inoculation of S.tricolor or S.tendae,which were isolated from the rhizosphere of a healthy individual in an infected field,significantly elevated(P<0.05)root mycorrhizal colonization,root biomass,fruit yield,and total K acquisitions of pepper and further significantly decreased(P<0.05)blight severity.According to the feature of enhancing disease-suppression by AM symbiosis,both S.tricolor and S.tendae were confirmed as MHB strains here.Specifically,S.tendae had a stronger performance in directly accelerating mycorrhization,while S.tricolor was also an antagonist to the pathogenic P.capsici.Furthermore,S.griseoaurantiacus with the independent disease-suppression function was not an MHB strain here.The redundancy analyses demonstrated that when AM fungus was present,root mycorrhizal colonization replaced soil pH becoming the main factor affecting pepper Phytophthora blight.Thus,S.tricolor and S.tendae seemed to have the value of preparation and application in the future to help AM symbiosis against pepper Phytophthora blight.展开更多
为筛选并鉴定黑松和美味牛肝菌菌根生长的辅助细菌(MHB),进一步探明根际微生物互作关系及其互作对黑松生长的作用,通过分离黑松-美味牛肝菌根际细菌、检测其胞外代谢产物对美味牛肝菌(Be)菌丝生长的促进作用,筛选出MHB潜力菌株。以MHB...为筛选并鉴定黑松和美味牛肝菌菌根生长的辅助细菌(MHB),进一步探明根际微生物互作关系及其互作对黑松生长的作用,通过分离黑松-美味牛肝菌根际细菌、检测其胞外代谢产物对美味牛肝菌(Be)菌丝生长的促进作用,筛选出MHB潜力菌株。以MHB潜力菌株对松苗的苗高、地径、茎根比、根侵染率的影响,筛选出菌根辅助细菌。通过细菌的形态、生化特性、16S r DNA序列鉴定细菌的种类。结果表明,菌株H12代谢产物可以显著促进Be菌丝生长,双接种Be+H12的黑松苗,苗高提高23.5%,地径提高50.5%,根侵染率提高了472.9%,茎根比下降37.9%。经鉴定,确定菌株H12为蜂房类芽孢杆菌(Paenibacillus alvei)。蜂房类芽孢杆菌对菌根的促生作用明显,可进一步开发为菌塘微生物菌剂。展开更多
以广西凭祥红锥-马尾松混交林中红锥和马尾松为研究对象,采集林下外生菌根和根际土壤,利用高通量测序分析该混交林下的菌根际微生物群落结构。结果表明,红锥、马尾松菌根际优势真菌为红菇属Russula、被孢霉属Mortierella、乳菇属Lactar...以广西凭祥红锥-马尾松混交林中红锥和马尾松为研究对象,采集林下外生菌根和根际土壤,利用高通量测序分析该混交林下的菌根际微生物群落结构。结果表明,红锥、马尾松菌根际优势真菌为红菇属Russula、被孢霉属Mortierella、乳菇属Lactarius、鹅膏属Amanita、拟锁瑚菌属Clavulinopsis、丝盖伞属Inocybe、锁瑚菌属Clavulina和木霉属Trichoderma,其中,Russula为绝对优势类群,菌根和根际中共生真菌均以外生菌根真菌为主。而优势细菌主要为常见菌根辅助细菌,如伯克霍尔德氏菌属Burkholderia、假单细胞菌属Pseudomonas、慢生根瘤菌属Bradyrhizobium、根瘤菌属Rhizobium和土壤杆菌属Agrobacterium,除芽孢杆菌属Bacillus外,菌根内菌根辅助细菌均高于根际。PICRUST功能分析表明红锥和马尾松菌根中部分膜运输通路(ABC transporters、transporters和secretion system ABC)和信号转导通路(two-component system)的丰度高于根际。α多样性分析表明,菌根和根际微生物多样性存在显著差异,马尾松菌根、根际真菌群落多样性显著高于红锥;β多样性分析表明两树种菌根和根际分别具有相似的微生物群落结构。优势菌群和土壤环境因子的RDA分析表明,土壤pH、全磷和全钾是影响菌根际菌群结构的主要环境因子。展开更多
基金funded by the National Natural Science Foundation of China(No.42177304)the National Key R&D Program of China(No.2022YFD1500202)supported by the Youth Innovation Promotion Association of Chinese Academy of Sciences(No.2016285)。
文摘Mycorrhiza helper bacteria(MHB)can promote the formation and functioning of arbuscular mycorrhizal(AM)symbiosis,but their role and application potential in coping with soil-borne diseases are still unclear.A 14-week greenhouse pot experiment was conducted to obtain several actinomycete strains helping AM symbiosis in suppressing the Phytophthora blight of pepper(Capsicum annuum L.),using a soil inoculated with Phytophthora capsici after sterilization.Five Streptomyces strains,including S.pseudogriseolus,S.albogriseolus,S.griseoaurantiacus,S.tricolor,and S.tendae,as well as the AM fungus(Funneliformis caledonium)were tested.The Phytophthora blight severity reached 66%at full productive stage in the uninoculated control,and inoculation of F.caledonium,S.griseoaurantiacus,and S.tricolor alone significantly decreased(P<0.05)it to 47%,40%,and 35%,respectively.Compared to F.caledonium alone,additional inoculation of S.tricolor or S.tendae,which were isolated from the rhizosphere of a healthy individual in an infected field,significantly elevated(P<0.05)root mycorrhizal colonization,root biomass,fruit yield,and total K acquisitions of pepper and further significantly decreased(P<0.05)blight severity.According to the feature of enhancing disease-suppression by AM symbiosis,both S.tricolor and S.tendae were confirmed as MHB strains here.Specifically,S.tendae had a stronger performance in directly accelerating mycorrhization,while S.tricolor was also an antagonist to the pathogenic P.capsici.Furthermore,S.griseoaurantiacus with the independent disease-suppression function was not an MHB strain here.The redundancy analyses demonstrated that when AM fungus was present,root mycorrhizal colonization replaced soil pH becoming the main factor affecting pepper Phytophthora blight.Thus,S.tricolor and S.tendae seemed to have the value of preparation and application in the future to help AM symbiosis against pepper Phytophthora blight.
文摘为筛选并鉴定黑松和美味牛肝菌菌根生长的辅助细菌(MHB),进一步探明根际微生物互作关系及其互作对黑松生长的作用,通过分离黑松-美味牛肝菌根际细菌、检测其胞外代谢产物对美味牛肝菌(Be)菌丝生长的促进作用,筛选出MHB潜力菌株。以MHB潜力菌株对松苗的苗高、地径、茎根比、根侵染率的影响,筛选出菌根辅助细菌。通过细菌的形态、生化特性、16S r DNA序列鉴定细菌的种类。结果表明,菌株H12代谢产物可以显著促进Be菌丝生长,双接种Be+H12的黑松苗,苗高提高23.5%,地径提高50.5%,根侵染率提高了472.9%,茎根比下降37.9%。经鉴定,确定菌株H12为蜂房类芽孢杆菌(Paenibacillus alvei)。蜂房类芽孢杆菌对菌根的促生作用明显,可进一步开发为菌塘微生物菌剂。
文摘以广西凭祥红锥-马尾松混交林中红锥和马尾松为研究对象,采集林下外生菌根和根际土壤,利用高通量测序分析该混交林下的菌根际微生物群落结构。结果表明,红锥、马尾松菌根际优势真菌为红菇属Russula、被孢霉属Mortierella、乳菇属Lactarius、鹅膏属Amanita、拟锁瑚菌属Clavulinopsis、丝盖伞属Inocybe、锁瑚菌属Clavulina和木霉属Trichoderma,其中,Russula为绝对优势类群,菌根和根际中共生真菌均以外生菌根真菌为主。而优势细菌主要为常见菌根辅助细菌,如伯克霍尔德氏菌属Burkholderia、假单细胞菌属Pseudomonas、慢生根瘤菌属Bradyrhizobium、根瘤菌属Rhizobium和土壤杆菌属Agrobacterium,除芽孢杆菌属Bacillus外,菌根内菌根辅助细菌均高于根际。PICRUST功能分析表明红锥和马尾松菌根中部分膜运输通路(ABC transporters、transporters和secretion system ABC)和信号转导通路(two-component system)的丰度高于根际。α多样性分析表明,菌根和根际微生物多样性存在显著差异,马尾松菌根、根际真菌群落多样性显著高于红锥;β多样性分析表明两树种菌根和根际分别具有相似的微生物群落结构。优势菌群和土壤环境因子的RDA分析表明,土壤pH、全磷和全钾是影响菌根际菌群结构的主要环境因子。
