Objective: To quantify the purine concentrations of the acupoints along the pericardium and nonpericardium meridians under healthy and myocardial ischemia conditions to investigate the relationship between acupoint pu...Objective: To quantify the purine concentrations of the acupoints along the pericardium and nonpericardium meridians under healthy and myocardial ischemia conditions to investigate the relationship between acupoint purine change and body functional status in rats.Methods: A total of 70 rats underwent an operation for myocardial ischemia,while 40 of them survived.They were randomly assigned to the following 5 subgroups:Neiguan(PC 6),Quze(PC 3),Tianquan(PC 2),Quchi(LI 11),and Jianyu(LI 15).Simultaneously,another40 healthy rats were also randomized into the same 5 subgroups as the control group.The tissue ?uids at the acupoints were collected by microdialysis for 30 min.Subsequently,the concentration of adenosine triphosphate(ATP),adenosine diphosphate(ADP),adenosine monophosphate(AMP),and adenosine(ADO) were quanti?ed using the high-performance liquid chromatography method.Results: Compared with the healthy group,the ADO at PC 6(P=0.012),PC 3(P=0.038),PC 2(P=0.024),and LI 15(P=0.042) obviously increased in the model group,while no signi?cant difference was observed at LI 11(P=0.201).However,ATP,ADP,and AMP manifested no significant changes in these areas,except for ATP at LI 15(P=0.036).Conclusions: Myocardial ischemia could induce an increase in ADO at acupoints of the upper arm and shoulder area,suggesting that the body functional status could affect the responsiveness of acupoints.The status of these acupoints could be pathogenically activated by disease,and distribution following some speci?c courses.展开更多
Objective： To observe the influence of electroacupuncture （EA） of different Yuan- Primary acupoints on cardiac sympathetic nerve activity （CSNA） and cardiac function in rabbits with acute myocardial ischemia （AM...Objective： To observe the influence of electroacupuncture （EA） of different Yuan- Primary acupoints on cardiac sympathetic nerve activity （CSNA） and cardiac function in rabbits with acute myocardial ischemia （AMI）, and to understand the interaction mechanisms of cardiac sympathetic nerve in adjusting acute myocardial ischemia. Method： A total of forty healthy rabbits were selected and 8 were randomly chosen as normal control. The rest 32 rabbits were inducted acute myocardial ischemia （AMI） models by intravenous Pituitrin, then the AMI model rabbits were randomly divided into 4 groups, 8 as model group, and 8 as EA on Shenmen （HT 7） group, 8 as EA on Taixi （K/3） group, and 8 as EA on nonpoint group. BIOPAC collection system of biological signal was used to record the changes of CSNA and cardiac function, and the data was analyzed using a SKY-A8 disposal system. Result： The lefl ventricular systolic peak pressure （LVSP）, maximum rate of rise of LVSP （＋dp/dt max） descended obviously, and maximum rate of descent of LVSP （-dp/dt max） ascended after induction of AMI. The ＋dp/dt max, -dp/dt max and LVSP of EA on Shenmen （HT 7） group are significantly higher than the untreated AMI group, EA on Taixi （KI 3） group and EA on nonpoint group after EA. The frequency of cardiac sympathetic nerve activity increased significantly after induction of AMI, and then decreased sharply （compared with that of normal group, P〈0.05）. Following EA, the cardiac sympathetic nerve activity of EA on Shenmen （HT 7） group are significantly higher than the model group, EA on Taixi （KI 3） group and EA on nonpoint group. And they have no significant difference with normal group. Conclusion： EA on Shenmen （HT 7） could improve the cardiac function and cardiac sympathetic nerve activity of rabbits with AMI, and promote the restoration of cardiac function. There is specificity in the effects of EA on different Yuan-Primary acupoints with the best effect on Shenmen （HT 7）, and no effect on Taixi （KI 3） and nonpoint. EA on related acupoint can adjust the cardiac sympathetic nerve activity, and may moderate the effects of myocardial ischemia. The sympathetic nerve is one of the key periphery ways in using acupuncture against myocardial ischemia.展开更多
基金Supported by National Natural Science Foundation of China(No.81590951,81373559,81373561 and 81573885)the State Key Program for Basic Research of China(No.2012CB518501)the Project of the Second Clinical Medical College of Nanjing University of Chinese Medicine(No.RLZZ201605)
文摘Objective: To quantify the purine concentrations of the acupoints along the pericardium and nonpericardium meridians under healthy and myocardial ischemia conditions to investigate the relationship between acupoint purine change and body functional status in rats.Methods: A total of 70 rats underwent an operation for myocardial ischemia,while 40 of them survived.They were randomly assigned to the following 5 subgroups:Neiguan(PC 6),Quze(PC 3),Tianquan(PC 2),Quchi(LI 11),and Jianyu(LI 15).Simultaneously,another40 healthy rats were also randomized into the same 5 subgroups as the control group.The tissue ?uids at the acupoints were collected by microdialysis for 30 min.Subsequently,the concentration of adenosine triphosphate(ATP),adenosine diphosphate(ADP),adenosine monophosphate(AMP),and adenosine(ADO) were quanti?ed using the high-performance liquid chromatography method.Results: Compared with the healthy group,the ADO at PC 6(P=0.012),PC 3(P=0.038),PC 2(P=0.024),and LI 15(P=0.042) obviously increased in the model group,while no signi?cant difference was observed at LI 11(P=0.201).However,ATP,ADP,and AMP manifested no significant changes in these areas,except for ATP at LI 15(P=0.036).Conclusions: Myocardial ischemia could induce an increase in ADO at acupoints of the upper arm and shoulder area,suggesting that the body functional status could affect the responsiveness of acupoints.The status of these acupoints could be pathogenically activated by disease,and distribution following some speci?c courses.
基金National Basic Research Program of China (973 Program, 2005CB523308)University Science Research Project of Anhui Province (KJ2009B200Z)
文摘Objective： To observe the influence of electroacupuncture （EA） of different Yuan- Primary acupoints on cardiac sympathetic nerve activity （CSNA） and cardiac function in rabbits with acute myocardial ischemia （AMI）, and to understand the interaction mechanisms of cardiac sympathetic nerve in adjusting acute myocardial ischemia. Method： A total of forty healthy rabbits were selected and 8 were randomly chosen as normal control. The rest 32 rabbits were inducted acute myocardial ischemia （AMI） models by intravenous Pituitrin, then the AMI model rabbits were randomly divided into 4 groups, 8 as model group, and 8 as EA on Shenmen （HT 7） group, 8 as EA on Taixi （K/3） group, and 8 as EA on nonpoint group. BIOPAC collection system of biological signal was used to record the changes of CSNA and cardiac function, and the data was analyzed using a SKY-A8 disposal system. Result： The lefl ventricular systolic peak pressure （LVSP）, maximum rate of rise of LVSP （＋dp/dt max） descended obviously, and maximum rate of descent of LVSP （-dp/dt max） ascended after induction of AMI. The ＋dp/dt max, -dp/dt max and LVSP of EA on Shenmen （HT 7） group are significantly higher than the untreated AMI group, EA on Taixi （KI 3） group and EA on nonpoint group after EA. The frequency of cardiac sympathetic nerve activity increased significantly after induction of AMI, and then decreased sharply （compared with that of normal group, P〈0.05）. Following EA, the cardiac sympathetic nerve activity of EA on Shenmen （HT 7） group are significantly higher than the model group, EA on Taixi （KI 3） group and EA on nonpoint group. And they have no significant difference with normal group. Conclusion： EA on Shenmen （HT 7） could improve the cardiac function and cardiac sympathetic nerve activity of rabbits with AMI, and promote the restoration of cardiac function. There is specificity in the effects of EA on different Yuan-Primary acupoints with the best effect on Shenmen （HT 7）, and no effect on Taixi （KI 3） and nonpoint. EA on related acupoint can adjust the cardiac sympathetic nerve activity, and may moderate the effects of myocardial ischemia. The sympathetic nerve is one of the key periphery ways in using acupuncture against myocardial ischemia.
