To investigate the effects of L-Tetrahydropalmatine (L-THP ) on neuron apoptosis during acute cerebral ischemia-reperfusion of rats and explore the effects of heat shock protein (HSP) on neuron apoptosis, Wistar rats ...To investigate the effects of L-Tetrahydropalmatine (L-THP ) on neuron apoptosis during acute cerebral ischemia-reperfusion of rats and explore the effects of heat shock protein (HSP) on neuron apoptosis, Wistar rats were randomly divided into 3 groups: normal group, ischemia- reperfusion group and treatment group. The condition of neuron apoptosis, the survival state of neuron, pathological changes under an electron microscope and the number of HSP70 positive cells were measured in all groups. Results showed that the apoptosis neuron number was increased obviously at the 24th h during reperfusion and was further increased at the 48th h, the 72th h. While the number of survival neurons was decreased gradually with the prolongation of reperfusion time. Treatment with L-THP could decrease the apoptosis neuron number but increase the survival neuron number and the HSP70 positive cell number. Our study suggested that L-THP could decrease apoptosis and necrosis of neuron, up-regulate the expression of HSP70 and protect the cerebral ischemic injury.展开更多
MicroRNA-208a(miR-208a)plays critical roles in the severe fibrosis and heart failure post myocardial ischemia/reperfusion(IR)injury.MiR-208a inhibitor(mI)with complementary RNA sequence can silence the expression of m...MicroRNA-208a(miR-208a)plays critical roles in the severe fibrosis and heart failure post myocardial ischemia/reperfusion(IR)injury.MiR-208a inhibitor(mI)with complementary RNA sequence can silence the expression of miR-208a,while it is challenging to achieve efficient and myocardium-targeted delivery.Herein,biomimetic nanocomplexes(NCs)reversibly coated with red blood cell membrane(RM)were developed for the myocardial delivery of mI.To construct the NCs,membrane-penetrating helical polypeptide(PG)was first adopted to condense mI and form the cationic inner core,which subsequently adsorbed catalase(CAT)via electrostatic interaction followed by surface coating with RM.The membrane-coated NCs enabled prolonged blood circulation after systemic administration,and could accumulate in the injured myocardium via passive targeting.In the oxidative microenvironment of injured myocardium,CAT decomposed H_(2)O_(2)to produce O_(2)bubbles,which drove the shedding of the outer RM to expose the positively charged inner core,thus facilitated effective internalization by cardiac cells.Based on the combined contribution of mI-mediated miR-208a silencing and CAT-mediated alleviation of oxidative stress,NCs effectively ameliorated the myocardial microenvironment,hence reducing the infarct size as well as fibrosis and promoting recovery of cardiac functions.This study provides an effective strategy for the cytosolic delivery of nucleic acid cargoes in the myocardium,and it renders an enlightened approach to resolve the blood circulation/cell internalization dilemma of cell membrane-coated delivery systems.展开更多
The occurrence of cardiovascular events is one of the important causes of death and disability in the nation.The ischemia and hypoxia of myocardial cells caused by coronary atherosclerosis,intravascular stenosis or my...The occurrence of cardiovascular events is one of the important causes of death and disability in the nation.The ischemia and hypoxia of myocardial cells caused by coronary atherosclerosis,intravascular stenosis or myocardial infarction leads to cell damage,necrosis and apoptosis,leading to Myocardial fibrosis affects cardiac function and the quality of life of patients.Reducing myocardial cell apoptosis,inhibiting myocardial fibrosis and promoting myocardial cell regeneration are of great significance for maintaining the normal shape and function of the heart.With the progress of research,scientists have discovered that the Hippo pathway plays an important role in the repair and regeneration of myocardial cells.This article reviews the regulatory effects and mechanisms of the various factors in the Hioop signaling pathway in the repair and regeneration of cardiomyocytes.展开更多
文摘To investigate the effects of L-Tetrahydropalmatine (L-THP ) on neuron apoptosis during acute cerebral ischemia-reperfusion of rats and explore the effects of heat shock protein (HSP) on neuron apoptosis, Wistar rats were randomly divided into 3 groups: normal group, ischemia- reperfusion group and treatment group. The condition of neuron apoptosis, the survival state of neuron, pathological changes under an electron microscope and the number of HSP70 positive cells were measured in all groups. Results showed that the apoptosis neuron number was increased obviously at the 24th h during reperfusion and was further increased at the 48th h, the 72th h. While the number of survival neurons was decreased gradually with the prolongation of reperfusion time. Treatment with L-THP could decrease the apoptosis neuron number but increase the survival neuron number and the HSP70 positive cell number. Our study suggested that L-THP could decrease apoptosis and necrosis of neuron, up-regulate the expression of HSP70 and protect the cerebral ischemic injury.
基金supported by the National Natural Science Foundation of China(Nos.82172076,52273144,and 52033006)111 project,Collaborative Innovation Center of Suzhou Nano Science&Technology,Joint International Research Laboratory of Carbon-Based Functional Materials and Devices,and Suzhou Key Laboratory of Nanotechnology and Biomedicine.
文摘MicroRNA-208a(miR-208a)plays critical roles in the severe fibrosis and heart failure post myocardial ischemia/reperfusion(IR)injury.MiR-208a inhibitor(mI)with complementary RNA sequence can silence the expression of miR-208a,while it is challenging to achieve efficient and myocardium-targeted delivery.Herein,biomimetic nanocomplexes(NCs)reversibly coated with red blood cell membrane(RM)were developed for the myocardial delivery of mI.To construct the NCs,membrane-penetrating helical polypeptide(PG)was first adopted to condense mI and form the cationic inner core,which subsequently adsorbed catalase(CAT)via electrostatic interaction followed by surface coating with RM.The membrane-coated NCs enabled prolonged blood circulation after systemic administration,and could accumulate in the injured myocardium via passive targeting.In the oxidative microenvironment of injured myocardium,CAT decomposed H_(2)O_(2)to produce O_(2)bubbles,which drove the shedding of the outer RM to expose the positively charged inner core,thus facilitated effective internalization by cardiac cells.Based on the combined contribution of mI-mediated miR-208a silencing and CAT-mediated alleviation of oxidative stress,NCs effectively ameliorated the myocardial microenvironment,hence reducing the infarct size as well as fibrosis and promoting recovery of cardiac functions.This study provides an effective strategy for the cytosolic delivery of nucleic acid cargoes in the myocardium,and it renders an enlightened approach to resolve the blood circulation/cell internalization dilemma of cell membrane-coated delivery systems.
基金National Natural Science Foundation of China Regional Fund Project(No.81960861)National Natural Science Foundation of China Regional Fund Project(No.81460712)Guangxi Key Scientific RESEARCH and Development Program Project Subject(No.Guike AB19110006)。
文摘The occurrence of cardiovascular events is one of the important causes of death and disability in the nation.The ischemia and hypoxia of myocardial cells caused by coronary atherosclerosis,intravascular stenosis or myocardial infarction leads to cell damage,necrosis and apoptosis,leading to Myocardial fibrosis affects cardiac function and the quality of life of patients.Reducing myocardial cell apoptosis,inhibiting myocardial fibrosis and promoting myocardial cell regeneration are of great significance for maintaining the normal shape and function of the heart.With the progress of research,scientists have discovered that the Hippo pathway plays an important role in the repair and regeneration of myocardial cells.This article reviews the regulatory effects and mechanisms of the various factors in the Hioop signaling pathway in the repair and regeneration of cardiomyocytes.
