In market economy,SNA(A System of National Accounts),GFS(A System of Government Finance Statistics)and MFS(Money Finance Summery)are the important tools in management of macro-economy In this paper,a comparison of SNA...In market economy,SNA(A System of National Accounts),GFS(A System of Government Finance Statistics)and MFS(Money Finance Summery)are the important tools in management of macro-economy In this paper,a comparison of SNA,GFS and MFS were made on the accounting rule,the accounting structure and the accounting general framework,and a analysis were made on the accounting body,the accounting objective,the system of indicators,the standard classification and the full accounts.展开更多
文章提出了一种新的基于MFS法(Method of Fundamental Solutions,即基本解法)的求解船舶势流问题的无网格数值方法。该方法采用势流控制方程的基本解表达流场中的速度势,可以得到任意阶连续的速度势导数;另外,通过引入流场虚边界的概念...文章提出了一种新的基于MFS法(Method of Fundamental Solutions,即基本解法)的求解船舶势流问题的无网格数值方法。该方法采用势流控制方程的基本解表达流场中的速度势,可以得到任意阶连续的速度势导数;另外,通过引入流场虚边界的概念避免了传统边界元方法中存在奇点的问题。相比传统的边界元方法,MFS法具有无奇性、不依赖网格、不涉及数值积分、数学原理简单和算法易于编程实现的优势。文中对于MFS法在求解浅水二维剖面水动力系数中的应用进行了探索,提出了一套实用的数值算法,并进一步结合频域切片法计算了Wigley船体的垂荡和纵摇响应,通过和频域面元法及试验结果的对比验证了方法的正确性和实用性;最后应用开发的程序分析了不同水深和航速对于耐波性的影响。该研究开创性地将MFS方法应用到了船舶水动力研究领域,为之后更深入广泛地应用这一特性优良的数值方法提供了有益的参考。展开更多
在家蚕品种C1(H)中发现了一种新的卵色突变体。该突变体卵色为淡红色,成虫复眼为红色,遗传分析表明该突变性状由1个隐性基因控制,可能与红卵突变基因re互为等位基因,命名为淡红卵突变体(pale red egg,re^p-1)。红卵突变体(re)被认为是MF...在家蚕品种C1(H)中发现了一种新的卵色突变体。该突变体卵色为淡红色,成虫复眼为红色,遗传分析表明该突变性状由1个隐性基因控制,可能与红卵突变基因re互为等位基因,命名为淡红卵突变体(pale red egg,re^p-1)。红卵突变体(re)被认为是MFS(major facilitator superfamily)基因的序列发生了改变,故对re^p-1突变体MFS基因的结构及表达进行分析。与C1(H)卵色正常型品系的MFS基因比较,re^p-1突变体的MFS基因在第6外显子中的一段长59 bp片段被长14 bp的片段所替换,但编码蛋白质的跨膜结构未发生根本改变,而re突变体中MFS基因的结构变化对表达产物的功能有显著影响。此外,利用qRT-PCR检测MFS基因在C1(H)的卵色正常型品系、re^p-1突变体和re突变体3个家蚕品系5龄幼虫各个组织中均有表达,但表达水平存在差异:相较于C1(H)卵色正常型品系和re^p-1突变体,re突变体所有组织中MFS基因的表达量普遍偏低;相较于C1(H)卵色正常型品系,re^p-1突变体的后部丝腺和精巢中MFS基因具有较高的表达量,在其他组织中的表达量无明显差异。上述结果将有助于对re^p-1突变基因定位分析和卵色突变形成机制的研究。展开更多
Fibroblasts are the most abundant cellular components of connective tissue. They possess phenotypical heterogenicity and may be present in the form of smooth muscle cells or myofibroblasts(MFs). MFs are spindle-shaped...Fibroblasts are the most abundant cellular components of connective tissue. They possess phenotypical heterogenicity and may be present in the form of smooth muscle cells or myofibroblasts(MFs). MFs are spindle-shaped cells with stress fibres and welldeveloped fibronexus,and they display α-smooth muscle actin immunohistochemically and smoothmuscle myofilaments ultrastructurally. MFs play a crucial role in physiological and pathological processes. Derived from various sources,they play pivotal roles not only by synthesizing and producing extracellular matrix components,such as other connective tissue cells,but also are involved in force production. In the tissue remodelling phase of wound closure,integrinmediated interactions between MFs and type I collagen result in scar tissue formation. The tumour stroma in oral cancer actively recruits various cell types into the tumour mass,where they act as different sources of MFs. This article reviews the importance of MFs and its role in pathological processes such as wound healing,odontogenic cysts and tumours,salivary gland tumours,oral preneoplasia,and oral squamous cell carcinoma. Research oriented on blocking the transdifferentiation of fibroblasts into MFs can facilitate the development of noninvasive therapeutic strategies for the treatment of fibrosis and/or cancer.展开更多
In order to investigate the roles of Wnt signal pathway in transformation of cardiac valvular myofibroblasts to the osteoblast-like phenotype, the primary cultured porcine aortic valve myofibroblasts were incubated wi...In order to investigate the roles of Wnt signal pathway in transformation of cardiac valvular myofibroblasts to the osteoblast-like phenotype, the primary cultured porcine aortic valve myofibroblasts were incubated with oxidized low density lipoprotein（ox-LDL, 50 mg/L）, and divided into four groups according to the ox-LDL treatment time： control group, ox-LDL 24-h group, ox-LDL 48-h group, and ox-LDL 72-h group. Wnt signal pathway blocker Dickkopf-1（DDK-1, 100 μg/L） was added in ox-LDL 72-h group. The expression of α-smooth muscle actin（α-SMA）, bone morphogenetic protein 2（BMP2）, alkaline phosphatase（ALP）, and osteogenic transcription factor Cbfa-1 was detected by Western blotting, and that of β-catenin, a key mediator of Wnt signal pathway by immunocytochemical staining method. The Wnt/β-catenin was observed and the transformation of myofibroblasts to the osteoblast-like phenotype was examined. The expression of α-SMA, BMP2, ALP and Cbfa-1 proteins in the control group was weaker than in the ox-LDL-treated groups. In ox-LDL-treated groups, the protein expression of α-SMA, BMP2, ALP, and Cbfa-1 was significantly increased in a time-dependent manner as compared with the control group, and there was significant difference among the three ox-LDL-treated groups（P〈0.05 for all）; β-catenin protein was also up-regulated in the ox-LDL-treated groups in a time-dependent manner as compared with the control group（P〈0.05）, and its transfer from cytoplasm to nucleus and accumulation in the nucleus were increased in the same fashion（P〈0.05）. After addition of DKK-1, the expression of α-SMA, bone-related proteins and β-catenin protein was significantly reduced as compared with ox-LDL 72-h group（P〈0.05）. The Wnt/ β-catenin signaling pathway may play an important role in transformation of valvular myofibroblasts to the osteoblast-like phenotype.展开更多
文摘In market economy,SNA(A System of National Accounts),GFS(A System of Government Finance Statistics)and MFS(Money Finance Summery)are the important tools in management of macro-economy In this paper,a comparison of SNA,GFS and MFS were made on the accounting rule,the accounting structure and the accounting general framework,and a analysis were made on the accounting body,the accounting objective,the system of indicators,the standard classification and the full accounts.
文摘文章提出了一种新的基于MFS法(Method of Fundamental Solutions,即基本解法)的求解船舶势流问题的无网格数值方法。该方法采用势流控制方程的基本解表达流场中的速度势,可以得到任意阶连续的速度势导数;另外,通过引入流场虚边界的概念避免了传统边界元方法中存在奇点的问题。相比传统的边界元方法,MFS法具有无奇性、不依赖网格、不涉及数值积分、数学原理简单和算法易于编程实现的优势。文中对于MFS法在求解浅水二维剖面水动力系数中的应用进行了探索,提出了一套实用的数值算法,并进一步结合频域切片法计算了Wigley船体的垂荡和纵摇响应,通过和频域面元法及试验结果的对比验证了方法的正确性和实用性;最后应用开发的程序分析了不同水深和航速对于耐波性的影响。该研究开创性地将MFS方法应用到了船舶水动力研究领域,为之后更深入广泛地应用这一特性优良的数值方法提供了有益的参考。
文摘Fibroblasts are the most abundant cellular components of connective tissue. They possess phenotypical heterogenicity and may be present in the form of smooth muscle cells or myofibroblasts(MFs). MFs are spindle-shaped cells with stress fibres and welldeveloped fibronexus,and they display α-smooth muscle actin immunohistochemically and smoothmuscle myofilaments ultrastructurally. MFs play a crucial role in physiological and pathological processes. Derived from various sources,they play pivotal roles not only by synthesizing and producing extracellular matrix components,such as other connective tissue cells,but also are involved in force production. In the tissue remodelling phase of wound closure,integrinmediated interactions between MFs and type I collagen result in scar tissue formation. The tumour stroma in oral cancer actively recruits various cell types into the tumour mass,where they act as different sources of MFs. This article reviews the importance of MFs and its role in pathological processes such as wound healing,odontogenic cysts and tumours,salivary gland tumours,oral preneoplasia,and oral squamous cell carcinoma. Research oriented on blocking the transdifferentiation of fibroblasts into MFs can facilitate the development of noninvasive therapeutic strategies for the treatment of fibrosis and/or cancer.
基金supported by grants from the National Nature Science Foundation of China(No.81070190)the Foundation of Natural Sciences of Hubei Province of China(No.2014CFB962)
文摘In order to investigate the roles of Wnt signal pathway in transformation of cardiac valvular myofibroblasts to the osteoblast-like phenotype, the primary cultured porcine aortic valve myofibroblasts were incubated with oxidized low density lipoprotein（ox-LDL, 50 mg/L）, and divided into four groups according to the ox-LDL treatment time： control group, ox-LDL 24-h group, ox-LDL 48-h group, and ox-LDL 72-h group. Wnt signal pathway blocker Dickkopf-1（DDK-1, 100 μg/L） was added in ox-LDL 72-h group. The expression of α-smooth muscle actin（α-SMA）, bone morphogenetic protein 2（BMP2）, alkaline phosphatase（ALP）, and osteogenic transcription factor Cbfa-1 was detected by Western blotting, and that of β-catenin, a key mediator of Wnt signal pathway by immunocytochemical staining method. The Wnt/β-catenin was observed and the transformation of myofibroblasts to the osteoblast-like phenotype was examined. The expression of α-SMA, BMP2, ALP and Cbfa-1 proteins in the control group was weaker than in the ox-LDL-treated groups. In ox-LDL-treated groups, the protein expression of α-SMA, BMP2, ALP, and Cbfa-1 was significantly increased in a time-dependent manner as compared with the control group, and there was significant difference among the three ox-LDL-treated groups（P〈0.05 for all）; β-catenin protein was also up-regulated in the ox-LDL-treated groups in a time-dependent manner as compared with the control group（P〈0.05）, and its transfer from cytoplasm to nucleus and accumulation in the nucleus were increased in the same fashion（P〈0.05）. After addition of DKK-1, the expression of α-SMA, bone-related proteins and β-catenin protein was significantly reduced as compared with ox-LDL 72-h group（P〈0.05）. The Wnt/ β-catenin signaling pathway may play an important role in transformation of valvular myofibroblasts to the osteoblast-like phenotype.
