本研究旨在对四引物扩增受阻突变体系PCR(T-ARMS PCR)方法进行拓展,将该方法应用于山羊肌肉生长抑制素(Myostain,MSTN)基因5 bp插入/缺失(Insertion/Deletion,InDel)变异的检测。结果显示,该方法可以明显的区分5 bp InDel变异的不同基因...本研究旨在对四引物扩增受阻突变体系PCR(T-ARMS PCR)方法进行拓展,将该方法应用于山羊肌肉生长抑制素(Myostain,MSTN)基因5 bp插入/缺失(Insertion/Deletion,InDel)变异的检测。结果显示,该方法可以明显的区分5 bp InDel变异的不同基因型,经Sanger测序验证,该方法检测的准确率为100%。利用该方法对577只安徽白山羊MSTN基因5 bp InDel位点进行检测和统计分析,结果显示该位点在安徽白山羊群体中有3种基因型,II(纯合插入型)、DD(野生型)和ID(杂合型),基因型频率分别为0.132、0.412和0.456。关联分析结果显示,该位点与羔羊的管围和育成羊的体重、体高及胸围显著相关。世界多个山羊品种该位点的频率分布情况显示,该位点在非洲山羊群体中变异频率较高,在亚洲山羊群体中变异程度较低。本研究利用T-ARMS PCR检测短片段InDel变异将为后续群体InDel位点的检测提供便利,同时也为山羊MSTN基因5 bp InDel的检测和标记辅助选择育种提供了基础。展开更多
To establish a rapid, accurate and economical real-time PCR assay system based on TaqMan probe technology for the detection of genetic variations of single nucleotide polymorphisms （SNPs） in myestatin （MSTN） gene,...To establish a rapid, accurate and economical real-time PCR assay system based on TaqMan probe technology for the detection of genetic variations of single nucleotide polymorphisms （SNPs） in myestatin （MSTN） gene, a pair of TaqMan probes were designed on the polymorphism loci of MSTN gene and used in PCR reaction system for SNP genotyping. Meanwhile, an association study was performed between MSTN genotypes and growth traits of Tan sheep, including birth weight, weaning weight, 3-month weight, and 6-month weight. The results showed that rs417816017 locus of MSTN gene in Tan sheep had two genotypes ： YY and XY. The individuals with genotype XY had a growth advantage over the ones with genotype YY. The results indicate that TaqMan probe-based real-time PCR assay can be used to detect the genotype of MSTN gene, which will provide candidate genes for breeding of Tan sheep.展开更多
In developed countries, study on special or candidate genes, which are useful for identifying species, breed and productivity of livestock, was conducted at high level and the results have already been used in practic...In developed countries, study on special or candidate genes, which are useful for identifying species, breed and productivity of livestock, was conducted at high level and the results have already been used in practice. Such advanced technology and innovation that we are facing is necessary to adopt in Mongolia. In this study, the myostatin gene （MSTN） was investigated as a candidate gene for meat animal in Mongolian breeds of cattle. The conventional phenol-chloroform method and FavorPrepTM tissue DNA extraction kit were used for DNA isolation, and the polymerase chain reaction （PCR） and sequencing analysis were used for further study. The nucleotide sequences of MSTN gene from Selenge, Kazakh white head breeds and Mongolian cattle were sequenced and reported on the DDBJ/EMBL/GenBank database （LC142726, LC146648, LC146649）, and Selenge breed showed the result of single nucleotide mutation in MSTN gene.展开更多
肌肉生长抑制素(Myostatin,MSTN)作为动物肌肉生长的负调节因子,对提高动物的肌肉量和瘦肉率具有重要意义。该文采用文献计量学方法,以"MSTN/GDF-8/Myostatin"为检索词对2000—2020年期间Web of Science核心合集数据库和CNKI...肌肉生长抑制素(Myostatin,MSTN)作为动物肌肉生长的负调节因子,对提高动物的肌肉量和瘦肉率具有重要意义。该文采用文献计量学方法,以"MSTN/GDF-8/Myostatin"为检索词对2000—2020年期间Web of Science核心合集数据库和CNKI中国学术期刊全文数据库收录的相关论文进行主题检索,并使用Excel 2016、GraphPad Prism 8.4.0和VOS viewer软件对数据进行可视化处理。结果显示,通过Web of Science平台共检索到相关MSTN的SCI论文4404篇,其中中国发表的论文675篇。CNKI中国学术期刊全文数据库内检索出759篇论文。全球关于MSTN的发文量在2000—2020年呈现总体上升的趋势,美国和一些畜牧业发达的国家对MSTN的研究较为深入;中国学者的发文量虽多但其篇均被引频次较低,需从不同维度开展该领域研究并加强机构间合作,提高文章水平,进而缩小差距。目前关于MSTN的研究集中于分子生物学、农学、遗传学、生物技术与微生物学四大领域。对于农学领域提高肉类品质和产量依旧十分重要,因此需要提高对MSTN如何影响肌肉发育及脂肪蓄积方面的认识。本文通过统计分析及可视化处理,为我国科研人员了解MSTN相关的研究动态和研究方向、并以此开展优良品种培育提供信息帮助。展开更多
The myostatin(MSTN)gene is considered a potential genetic marker to improve economically important traits in live-stock,since the discovery of its function using the MSTN knockout mice.The anti-myogenic function of th...The myostatin(MSTN)gene is considered a potential genetic marker to improve economically important traits in live-stock,since the discovery of its function using the MSTN knockout mice.The anti-myogenic function of the MSTN gene was further demonstrated in farm animal species with natural or induced mutations.In poultry species,myo-genesis in cell culture was regulated by modulation of the MSTN gene.Also,different expression levels of the MSTN gene in poultry models with different muscle mass have been reported,indicating the conserved myogenic function of the MSTN gene between mammalian and avian species.Recent advances of CRISPR/Cas9-mediated genome edit-ing techniques have led to development of genome-edited poultry species targeting the MSTN gene to clearly dem-onstrate its anti-myogenic function and further investigate other potential functions in poultry species.This review summarizes research conducted to understand the function of the MSTN gene in various poultry models from cells to whole organisms.Furthermore,the genome-edited poultry models targeting the MSTN gene are reviewed to inte-grate diverse effects of the MSTN gene on different traits of poultry species.展开更多
文摘本研究旨在对四引物扩增受阻突变体系PCR(T-ARMS PCR)方法进行拓展,将该方法应用于山羊肌肉生长抑制素(Myostain,MSTN)基因5 bp插入/缺失(Insertion/Deletion,InDel)变异的检测。结果显示,该方法可以明显的区分5 bp InDel变异的不同基因型,经Sanger测序验证,该方法检测的准确率为100%。利用该方法对577只安徽白山羊MSTN基因5 bp InDel位点进行检测和统计分析,结果显示该位点在安徽白山羊群体中有3种基因型,II(纯合插入型)、DD(野生型)和ID(杂合型),基因型频率分别为0.132、0.412和0.456。关联分析结果显示,该位点与羔羊的管围和育成羊的体重、体高及胸围显著相关。世界多个山羊品种该位点的频率分布情况显示,该位点在非洲山羊群体中变异频率较高,在亚洲山羊群体中变异程度较低。本研究利用T-ARMS PCR检测短片段InDel变异将为后续群体InDel位点的检测提供便利,同时也为山羊MSTN基因5 bp InDel的检测和标记辅助选择育种提供了基础。
基金Supported by Special Fund for Science and Technology Development of Ningxia Hui Autonomous Region(2012ZDN1001)Domestic Animal Germplasm Resources Sharing Platform Project(2005DKA21101)
文摘To establish a rapid, accurate and economical real-time PCR assay system based on TaqMan probe technology for the detection of genetic variations of single nucleotide polymorphisms （SNPs） in myestatin （MSTN） gene, a pair of TaqMan probes were designed on the polymorphism loci of MSTN gene and used in PCR reaction system for SNP genotyping. Meanwhile, an association study was performed between MSTN genotypes and growth traits of Tan sheep, including birth weight, weaning weight, 3-month weight, and 6-month weight. The results showed that rs417816017 locus of MSTN gene in Tan sheep had two genotypes ： YY and XY. The individuals with genotype XY had a growth advantage over the ones with genotype YY. The results indicate that TaqMan probe-based real-time PCR assay can be used to detect the genotype of MSTN gene, which will provide candidate genes for breeding of Tan sheep.
文摘In developed countries, study on special or candidate genes, which are useful for identifying species, breed and productivity of livestock, was conducted at high level and the results have already been used in practice. Such advanced technology and innovation that we are facing is necessary to adopt in Mongolia. In this study, the myostatin gene （MSTN） was investigated as a candidate gene for meat animal in Mongolian breeds of cattle. The conventional phenol-chloroform method and FavorPrepTM tissue DNA extraction kit were used for DNA isolation, and the polymerase chain reaction （PCR） and sequencing analysis were used for further study. The nucleotide sequences of MSTN gene from Selenge, Kazakh white head breeds and Mongolian cattle were sequenced and reported on the DDBJ/EMBL/GenBank database （LC142726, LC146648, LC146649）, and Selenge breed showed the result of single nucleotide mutation in MSTN gene.
文摘肌肉生长抑制素(Myostatin,MSTN)作为动物肌肉生长的负调节因子,对提高动物的肌肉量和瘦肉率具有重要意义。该文采用文献计量学方法,以"MSTN/GDF-8/Myostatin"为检索词对2000—2020年期间Web of Science核心合集数据库和CNKI中国学术期刊全文数据库收录的相关论文进行主题检索,并使用Excel 2016、GraphPad Prism 8.4.0和VOS viewer软件对数据进行可视化处理。结果显示,通过Web of Science平台共检索到相关MSTN的SCI论文4404篇,其中中国发表的论文675篇。CNKI中国学术期刊全文数据库内检索出759篇论文。全球关于MSTN的发文量在2000—2020年呈现总体上升的趋势,美国和一些畜牧业发达的国家对MSTN的研究较为深入;中国学者的发文量虽多但其篇均被引频次较低,需从不同维度开展该领域研究并加强机构间合作,提高文章水平,进而缩小差距。目前关于MSTN的研究集中于分子生物学、农学、遗传学、生物技术与微生物学四大领域。对于农学领域提高肉类品质和产量依旧十分重要,因此需要提高对MSTN如何影响肌肉发育及脂肪蓄积方面的认识。本文通过统计分析及可视化处理,为我国科研人员了解MSTN相关的研究动态和研究方向、并以此开展优良品种培育提供信息帮助。
基金funded by the United States Department of Agricul-ture National Institute of Food and Agriculture Grant(Project No.2020-67030-31338)。
文摘The myostatin(MSTN)gene is considered a potential genetic marker to improve economically important traits in live-stock,since the discovery of its function using the MSTN knockout mice.The anti-myogenic function of the MSTN gene was further demonstrated in farm animal species with natural or induced mutations.In poultry species,myo-genesis in cell culture was regulated by modulation of the MSTN gene.Also,different expression levels of the MSTN gene in poultry models with different muscle mass have been reported,indicating the conserved myogenic function of the MSTN gene between mammalian and avian species.Recent advances of CRISPR/Cas9-mediated genome edit-ing techniques have led to development of genome-edited poultry species targeting the MSTN gene to clearly dem-onstrate its anti-myogenic function and further investigate other potential functions in poultry species.This review summarizes research conducted to understand the function of the MSTN gene in various poultry models from cells to whole organisms.Furthermore,the genome-edited poultry models targeting the MSTN gene are reviewed to inte-grate diverse effects of the MSTN gene on different traits of poultry species.
