The present study was conducted to assess the molecular characterization and genetic diversity amongst natural populations of Myrica rubra in Guangxi Zhuang Autonomous Region, China, thus to provide scientific evidenc...The present study was conducted to assess the molecular characterization and genetic diversity amongst natural populations of Myrica rubra in Guangxi Zhuang Autonomous Region, China, thus to provide scientific evidence for germplasm conservation and exploitation. Using ISSR （inter-simple sequence repeats） markers, the level of genetic variation and the molecular characterization of 10 natural populations of M. rubra, originated from Guangxi Zhuang Autonomous Region in China, were performed. Based on 11 primers, 123 clear and reproducible DNA fragments were generated, of which 95 （77.24%） were polymorphic. The average value of Nei＇s gene diversity （He） was 0.268. The coefficient of genetic differentiation （Gst） was 0.341, revealing that 34.1% of the total molecular variance existed among populations. The Mantel statistical testing showed that the genetic distance was correlated to the geographic distance, but the correlation was not significant. Ten populations were divided into two big clusters according to unweighted pair group method with arithmetic mean （UPGMA） analysis. One consisted of populations of Rongxian （RX）, Hepu （HP）, Liangqing （LQ）, Marshan （MS）, Lingshan （LS） and Shansi （SS）, which originated from the southern Guangxi, while the other was composed of Guanyang （GY） and Lingui （LG） populations of northern Guangxi, Huanjiang （HJ） populations of northwestern Guangxi and Shanglin （SL） populations of southern Guangxi. The level of genetic variation in wild M. rubra population distributed in Guangxi is high. Gene drift within the population was responsible for genetic variation in wild M. rubra in Guangxi, and the effect of the genetic flow among inter-populations was not significant. Classification of wild M. rubra populations was correlated to climate and environment. The molecular characterization and diversity assessment of M. rubra is of immense value for planning conservation of its genetic resources and their exploitation for further studies.展开更多
A new diarylheptanoid, 3,5-dimethoxy1-17-hydroxyl-4, 11, 19-triketo-[7,0]meta-cyclophane, named as rubanone 1, was isolated from the bark of Myrica rubra along with four known compounds 2-5. Their structures were eluc...A new diarylheptanoid, 3,5-dimethoxy1-17-hydroxyl-4, 11, 19-triketo-[7,0]meta-cyclophane, named as rubanone 1, was isolated from the bark of Myrica rubra along with four known compounds 2-5. Their structures were elucidated by various spectroscopic methods including 2D-NMR techniques or comparison with authentic samples.展开更多
杨梅(Myrica rubra Sieb et Zucc.)是浙江著名的特色水果之一,浙江的气候比较适宜杨梅种植,但由于区域布局不合理制约了杨梅的发展。根据杨梅的生物学特性,筛选出了杨梅种植的气候区划指标,并建立了各区划指标与经度、纬度和海拔高度的...杨梅(Myrica rubra Sieb et Zucc.)是浙江著名的特色水果之一,浙江的气候比较适宜杨梅种植,但由于区域布局不合理制约了杨梅的发展。根据杨梅的生物学特性,筛选出了杨梅种植的气候区划指标,并建立了各区划指标与经度、纬度和海拔高度的空间分析模型。借助浙江省1︰25万地形数据、数字高程模型(DEM)和数字化土壤图,综合考虑气候因子、坡度、坡向、海拔高度、土壤类型和土壤质地等要素,采用加权指数求和法,对11个区划指标进行量化分级,建立杨梅栽培区划指标判断矩阵。将11个区划指标适宜性等级图按照权重进行叠加分析,得到综合指数分布图。同时参照浙江省土地利用现状,将不适宜杨梅种植的地块屏蔽,分析综合指数分布规律,将浙江省杨梅种植综合气候区划划分为最适宜区、适宜区和不适宜区3个等级。最适宜种植区占评价区域面积的25.15%,主要集中在宁波、台州、温州、丽水、金华、杭州和绍兴地区的低丘陵地带;适宜区占参评土地面积的46.37%,主要分布在丽水、温州、金华、杭州和衢州等海拔高度为500～700m的半高山区;不适宜区占评价区域面积的28.48%,主要在丽水市和杭州市的海拔在700m以上的高山地区。本研究以期能为浙江特色作物的规划和杨梅产业的持续发展提供科学依据。展开更多
采集戴云山脉自然群体的41份野生杨梅样品为材料,采用ISSR分子标记结合相关软件进行遗传结构分析。研究结果表明:筛选的13条引物共扩增获得175条DNA条带,其中多态性条带为140条,占扩增总片段的80%,表明戴云山野生杨梅具有较高的遗传多...采集戴云山脉自然群体的41份野生杨梅样品为材料,采用ISSR分子标记结合相关软件进行遗传结构分析。研究结果表明:筛选的13条引物共扩增获得175条DNA条带,其中多态性条带为140条,占扩增总片段的80%,表明戴云山野生杨梅具有较高的遗传多样性水平;STRUCTURE聚类分析(基于模型)将41份野生杨梅划分为6个类群,结合Q值(Inferred ancestry of individuals)分析表明,戴云山脉野生杨梅存在丰富的遗传信息交流。展开更多
基于Web of Science数据库,采用文献计量学方法,分别对世界上研究最前沿的20个研究机构、研究作者、学术期刊、学科和国家进行了文献计量学统计,进而了解2004—2014年杨梅研究概况和动态。结果表明:2004—2014年世界杨梅研究论文数量共...基于Web of Science数据库,采用文献计量学方法,分别对世界上研究最前沿的20个研究机构、研究作者、学术期刊、学科和国家进行了文献计量学统计,进而了解2004—2014年杨梅研究概况和动态。结果表明:2004—2014年世界杨梅研究论文数量共163篇;文献产出量逐年增加,2013年文献数量最高;中国、日本、美国、澳大利亚、新西兰文献数量居世界前5位,中国文献数量最多,为108篇;浙江大学、浙江农业科学院、中国科学院、广东药学院、京都大学杨梅文献较多;最有学术影响力的核心作者来自中国,主要核心期刊是Food Chemistry、Journal of Agricultural and Food Chemistry、Journal of Natural Products、Scientia Horticulturae、BMC Genomics;主要学科是食品科学技术、植物科学、应用化学、化学药物、药理学。展开更多
基金supported by the National Natural Science Foundation of China (30560007)
文摘The present study was conducted to assess the molecular characterization and genetic diversity amongst natural populations of Myrica rubra in Guangxi Zhuang Autonomous Region, China, thus to provide scientific evidence for germplasm conservation and exploitation. Using ISSR （inter-simple sequence repeats） markers, the level of genetic variation and the molecular characterization of 10 natural populations of M. rubra, originated from Guangxi Zhuang Autonomous Region in China, were performed. Based on 11 primers, 123 clear and reproducible DNA fragments were generated, of which 95 （77.24%） were polymorphic. The average value of Nei＇s gene diversity （He） was 0.268. The coefficient of genetic differentiation （Gst） was 0.341, revealing that 34.1% of the total molecular variance existed among populations. The Mantel statistical testing showed that the genetic distance was correlated to the geographic distance, but the correlation was not significant. Ten populations were divided into two big clusters according to unweighted pair group method with arithmetic mean （UPGMA） analysis. One consisted of populations of Rongxian （RX）, Hepu （HP）, Liangqing （LQ）, Marshan （MS）, Lingshan （LS） and Shansi （SS）, which originated from the southern Guangxi, while the other was composed of Guanyang （GY） and Lingui （LG） populations of northern Guangxi, Huanjiang （HJ） populations of northwestern Guangxi and Shanglin （SL） populations of southern Guangxi. The level of genetic variation in wild M. rubra population distributed in Guangxi is high. Gene drift within the population was responsible for genetic variation in wild M. rubra in Guangxi, and the effect of the genetic flow among inter-populations was not significant. Classification of wild M. rubra populations was correlated to climate and environment. The molecular characterization and diversity assessment of M. rubra is of immense value for planning conservation of its genetic resources and their exploitation for further studies.
文摘A new diarylheptanoid, 3,5-dimethoxy1-17-hydroxyl-4, 11, 19-triketo-[7,0]meta-cyclophane, named as rubanone 1, was isolated from the bark of Myrica rubra along with four known compounds 2-5. Their structures were elucidated by various spectroscopic methods including 2D-NMR techniques or comparison with authentic samples.
文摘杨梅(Myrica rubra Sieb et Zucc.)是浙江著名的特色水果之一,浙江的气候比较适宜杨梅种植,但由于区域布局不合理制约了杨梅的发展。根据杨梅的生物学特性,筛选出了杨梅种植的气候区划指标,并建立了各区划指标与经度、纬度和海拔高度的空间分析模型。借助浙江省1︰25万地形数据、数字高程模型(DEM)和数字化土壤图,综合考虑气候因子、坡度、坡向、海拔高度、土壤类型和土壤质地等要素,采用加权指数求和法,对11个区划指标进行量化分级,建立杨梅栽培区划指标判断矩阵。将11个区划指标适宜性等级图按照权重进行叠加分析,得到综合指数分布图。同时参照浙江省土地利用现状,将不适宜杨梅种植的地块屏蔽,分析综合指数分布规律,将浙江省杨梅种植综合气候区划划分为最适宜区、适宜区和不适宜区3个等级。最适宜种植区占评价区域面积的25.15%,主要集中在宁波、台州、温州、丽水、金华、杭州和绍兴地区的低丘陵地带;适宜区占参评土地面积的46.37%,主要分布在丽水、温州、金华、杭州和衢州等海拔高度为500～700m的半高山区;不适宜区占评价区域面积的28.48%,主要在丽水市和杭州市的海拔在700m以上的高山地区。本研究以期能为浙江特色作物的规划和杨梅产业的持续发展提供科学依据。
文摘采集戴云山脉自然群体的41份野生杨梅样品为材料,采用ISSR分子标记结合相关软件进行遗传结构分析。研究结果表明:筛选的13条引物共扩增获得175条DNA条带,其中多态性条带为140条,占扩增总片段的80%,表明戴云山野生杨梅具有较高的遗传多样性水平;STRUCTURE聚类分析(基于模型)将41份野生杨梅划分为6个类群,结合Q值(Inferred ancestry of individuals)分析表明,戴云山脉野生杨梅存在丰富的遗传信息交流。
文摘基于Web of Science数据库,采用文献计量学方法,分别对世界上研究最前沿的20个研究机构、研究作者、学术期刊、学科和国家进行了文献计量学统计,进而了解2004—2014年杨梅研究概况和动态。结果表明:2004—2014年世界杨梅研究论文数量共163篇;文献产出量逐年增加,2013年文献数量最高;中国、日本、美国、澳大利亚、新西兰文献数量居世界前5位,中国文献数量最多,为108篇;浙江大学、浙江农业科学院、中国科学院、广东药学院、京都大学杨梅文献较多;最有学术影响力的核心作者来自中国,主要核心期刊是Food Chemistry、Journal of Agricultural and Food Chemistry、Journal of Natural Products、Scientia Horticulturae、BMC Genomics;主要学科是食品科学技术、植物科学、应用化学、化学药物、药理学。
