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Grain yield and N uptake of maize in response to increased plant density under reduced water and nitrogen supply conditions 被引量:3
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作者 Jingui Wei Qiang Chai +5 位作者 Wen Yin Hong Fan Yao Guo Falong Hu Zhilong Fan QimingWang 《Journal of Integrative Agriculture》 SCIE CSCD 2024年第1期122-140,共19页
The development of modern agriculture requires the reduction of water and chemical N fertilizer inputs.Increasing the planting density can maintain higher yields,but also consumes more of these restrictive resources.H... The development of modern agriculture requires the reduction of water and chemical N fertilizer inputs.Increasing the planting density can maintain higher yields,but also consumes more of these restrictive resources.However,whether an increased maize density can compensate for the negative effects of reduced water and N supply on grain yield and N uptake in the arid irrigated areas remains unknown.This study is part of a long-term positioning trial that started in 2016.A split-split plot field experiment of maize was implemented in the arid irrigated area of northwestern China in 2020 to 2021.The treatments included two irrigation levels:local conventional irrigation reduced by 20%(W1,3,240 m^(3)ha^(-1))and local conventional irrigation(W2,4,050 m^(3)ha^(-1));two N application rates:local conventional N reduced by 25%(N1,270 kg ha^(-1))and local conventional N(360 kg ha^(-1));and three planting densities:local conventional density(D1,75,000 plants ha^(-1)),density increased by 30%(D2,97,500 plants ha-1),and density increased by 60%(D3,120,000 plants ha^(-1)).Our results showed that the grain yield and aboveground N accumulation of maize were lower under the reduced water and N inputs,but increasing the maize density by 30% can compensate for the reductions of grain yield and aboveground N accumulation caused by the reduced water and N supply.When water was reduced while the N application rate remained unchanged,increasing the planting density by 30% enhanced grain yield by 13.9% and aboveground N accumulation by 15.3%.Under reduced water and N inputs,increasing the maize density by 30% enhanced N uptake efficiency and N partial factor productivity,and it also compensated for the N harvest index and N metabolic related enzyme activities.Compared with W2N2D1,the N uptake efficiency and N partial factor productivity increased by 28.6 and 17.6%under W1N1D2.W1N2D2 had 8.4% higher N uptake efficiency and 13.9% higher N partial factor productivity than W2N2D1.W1N2D2 improved urease activity and nitrate reductase activity by 5.4% at the R2(blister)stage and 19.6% at the V6(6th leaf)stage,and increased net income and the benefit:cost ratio by 22.1 and 16.7%,respectively.W1N1D2 and W1N2D2 reduced the nitrate nitrogen and ammoniacal nitrogen contents at the R6 stage in the 40-100 cm soil layer,compared with W2N2D1.In summary,increasing the planting density by 30% can compensate for the loss of grain yield and aboveground N accumulation under reduced water and N inputs.Meanwhile,increasing the maize density by 30% improved grain yield and aboveground N accumulation when water was reduced by 20% while the N application rate remained constant in arid irrigation areas. 展开更多
关键词 water and n reduction plant density MAIZE grain yield n uptake compensation effect
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Legume green manure can intensify the function of chemical nitrogen fertilizer substitution via increasing nitrogen supply and uptake of wheat
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作者 Jingui Wei Zhilong Fan +5 位作者 Falong Hu Shoufa Mao Fang Yin Qiming Wang Qiang Chai Wen Yin 《The Crop Journal》 SCIE CSCD 2024年第4期1222-1232,共11页
Achieving the green development of agriculture requires the reduction of chemical nitrogen(N)fertilizer input.Previous studies have confirmed that returning green manure to the field is an effective measure to improve... Achieving the green development of agriculture requires the reduction of chemical nitrogen(N)fertilizer input.Previous studies have confirmed that returning green manure to the field is an effective measure to improve crop yields while substituting partial chemical N fertilizer.However,it remains unclear how to further intensify the substituting function of green manure and elucidate its underlying agronomic mechanism.In a split-plot field experiment in spring wheat,different green manures returned to the field under reduced chemical N supply was established in an oasis area since 2018,in order to investigate the effect of green manure and reduced N on grain yield,N uptake,N use efficiency(NUE),N nutrition index,soil organic matter,and soil N of wheat in 2020-2022.Our results showed that mixed sown common vetch and hairy vetch can substitute 40%of chemical N fertilizer without reducing grain yield or N accumulation.Noteworthily,mixed sown common vetch and hairy vetch under reduced N by 20%showed the highest N agronomy efficiency and recovery efficiency,which were 92.0%and 46.0%higher than fallow after wheat harvest and conventional N application rate,respectively.The increase in NUE of wheat was mainly attributed to mixed sown common vetch and hairy vetch,which increased N transportation quantity and transportation rate at pre-anthesis,enhanced N harvest index,optimized N nutrition index,and increased activities of nitrate reductase and glutamine synthetase of leaf,respectively.Meanwhile,mixed sown common vetch and hairy vetch under reduced N by 20%improved soil organic matter and N contents.Therefore,mixed sown common vetch and hairy vetch can substitute 40%of chemical N fertilizer while maintaining grain yield and N accumulation,and it combined with reduced chemical N by 20%or 40%improved NUE of wheat via enhancing N supply and uptake. 展开更多
关键词 Legume green manure Reduced n input WHEAT n uptake and supply Agronomic mechanism
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The preparation and properties of N-doped carbon materials and their use for sodium storage
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作者 YUAN Ren-lu HOU Ruo-yang +4 位作者 SHANG Lei LIU Xue-wei LI Ang CHEN Xiao-hong SONG Huai-he 《新型炭材料(中英文)》 SCIE EI CAS CSCD 北大核心 2024年第5期770-795,共26页
Defect engineering by heteroatom doping gives carbon materials some new characteristics such as a different electronic structure and a high electrochemical activity,making them suitable for high-performance applicatio... Defect engineering by heteroatom doping gives carbon materials some new characteristics such as a different electronic structure and a high electrochemical activity,making them suitable for high-performance applications.N-doping has been widely investigated because of its similar atom radius to carbon,high electronegativity as well as many different configurations.We summarize the preparation methods and properties of N-doped carbon materials,and discuss their possible use in sodium ion storage.The relationships between N content/configuration and crystallinity,electronic conductivity,wettability,chemical reactivity as well as sodium ion storage performance are discussed. 展开更多
关键词 n-doped carbon material n configuration Preparation method Performance Sodium storage
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Vacancy defect MoSeTe embedded in N and F co-doped carbon skeleton for high performance sodium ion batteries and hybrid capacitors
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作者 Dehui Yang Wentao Guo +6 位作者 Fei Guo Jiaming Zhu Gang Wang Hui Wang Guanghui Yuan Shenghua Ma Beibei Wang 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2024年第3期652-664,I0014,共14页
Sodium-ion batteries(SIBs) and hybrid capacitors(SIHCs) have garnered significant attention in energy storage due to their inherent advantages,including high energy density,cost-effectiveness,and enhanced safety.Howev... Sodium-ion batteries(SIBs) and hybrid capacitors(SIHCs) have garnered significant attention in energy storage due to their inherent advantages,including high energy density,cost-effectiveness,and enhanced safety.However,developing high-performance anode materials to improve sodium storage performa nce still remains a major challenge.Here,a facile one-pot method has been developed to fabricate a hybrid of MoSeTe nanosheets implanted within the N,F co-doped honeycomb carbon skeleton(MoSeTe/N,F@C).Experimental results demonstrate that the incorporation of large-sized Te atoms into MoSeTe nanosheets enlarges the layer spacing and creates abundant anion vacancies,which effectively facilitate the insertion/extraction of Na^(+) and provide numerous ion adsorption sites for rapid surface capacitive behavior.Additionally,the heteroatoms N,F co-doped honeycomb carbon skeleton with a highly conductive network can restrain the volume expansion and boost reaction kinetics within the electrode.As anticipated,the MoSeTe/N,F@C anode exhibits high reversible capacities along with exceptional cycle stability.When coupled with Na_(3)V_(2)(PO_(4))_(3)@C(NVPF@C) to form SIB full cells,the anode delivers a reversible specific capacity of 126 mA h g^(-1) after 100 cycles at 0.1 A g^(-1).Furthermore,when combined with AC to form SIHC full cells,the anode demonstrates excellent cycling stability with a reversible specific capacity of50 mA h g^(-1) keeping over 3700 cycles at 1.0 A g^(-1).In situ XRD,ex situ TEM characterization,and theoretical calculations(DFT) further confirm the reversibility of sodium storage in MoSeTe/N,F@C anode materials during electrochemical reactions,highlighting their potential for widespread practical application.This work provides new insights into the promising utilization of advanced transition metal dichalcogenides as anode materials for Na^(+)-based energy storage devices. 展开更多
关键词 MoSeTe n F co-doped honeycomb carbon skeleton Sodium-ion batteries Sodium-ion hybrid capacitor
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Heteroatom tuning in agarose derived carbon aerogel for enhanced potassium ion multiple energy storage
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作者 Kaijun Xie Xin Liu +5 位作者 Haolin Li Long Fang Kai Xia Dongjiang Yang Yihui Zou Xiaodong Zhang 《Carbon Energy》 SCIE EI CAS CSCD 2024年第3期239-254,共16页
The incorporation of heteroatoms into carbon aerogels(CAs)can lead to structural distortions and changes in active sites due to their smaller size and electronegativity compared to pure carbon.However,the evolution of... The incorporation of heteroatoms into carbon aerogels(CAs)can lead to structural distortions and changes in active sites due to their smaller size and electronegativity compared to pure carbon.However,the evolution of the electronic structure from single-atom doping to heteroatom codoping in CAs has not yet been thoroughly investigated,and the impact of codoping on potassium ion(K+)storage and diffusion pathways as electrode material remains unclear.In this study,experimental and theoretical simulations were conducted to demonstrate that heteroatom codoping,composed of multiple heteroatoms(O/N/B)with different properties,has the potential to improve the electrical properties and stability of CAs compared to single-atom doping.Electronic states near the Fermi level have revealed that doping with O/N/B generates a greater number of active centers on adjacent carbon atoms than doping with O and O/N atoms.As a result of synergy with enhanced wetting ability(contact angle of 9.26°)derived from amino groups and hierarchical porous structure,ON-CA has the most optimized adsorption capacity(−1.62 eV)and diffusion barrier(0.12 eV)of K^(+).The optimal pathway of K^(+)in ON-CA is along the carbon ring with N or O doping.As K^(+)storage material for supercapacitors and ion batteries,it shows an outstanding specific capacity and capacitance,electrochemical stability,and rate performance.Especially,the assembled symmetrical K^(+)supercapacitor demonstrates an energy density of 51.8 Wh kg^(−1),an ultrahigh power density of 443Wkg^(−1),and outstanding cycling stability(maintaining 83.3%after 10,000 cycles in 1M KPF6 organic electrolyte).This research provides valuable insights into the design of highperformance potassium ion storage materials. 展开更多
关键词 AGAROSE carbon aerogels O/n/B codoping potassium-ion battery potassium-ion supercapacitor
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Establishment of High-sensitivity Rapid Fluorescence Quantitative Detection Method for Antibody against Peste des Petits Ruminants Virus
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作者 Zhao LIU Bo LIU +3 位作者 Zhida LIN Hang SUN Yu SUN Xiaohui SONG 《Agricultural Biotechnology》 2024年第5期22-27,共6页
[Objectives]This study was conducted to establish a rapid quantitative method for detecting antibody against Peste des Petits Ruminants Virus(PPR V)in sheep serum.[Methods]Soluble N protein and NH fusion protein were ... [Objectives]This study was conducted to establish a rapid quantitative method for detecting antibody against Peste des Petits Ruminants Virus(PPR V)in sheep serum.[Methods]Soluble N protein and NH fusion protein were obtained in Escherichia coli prokaryotic expression system by optimizing codons and expression conditions of E.coli.Furthermore,based on the purified soluble N protein and NH fusion protein,a high-sensitivity fluorescence immunoassay kit for detecting the antibody against PPR V was established.[Results]The method could quickly and quantitatively detect PPR V antibody in sheep serum,with high sensitivity and specificity,without any cross reaction to other related sheep pathogens.The intra-batch and inter-batch coefficients of variation were less than 10%and 15%,respectively,and the method had good repeatability.Through detection on 292 clinical serum samples,it was compared with the French IDVET competitive ELISA kit,and the coincidence rate of the two methods reached 93.84%.Compared with the serum neutralization test,the detected titer value of the high-sensitivity rapid fluorescence quantitative detection method was basically consistent with the tilter value obtained by the neutralization test on the standard positive serum(provided by the WOAH Brucellosis Reference Laboratory of France).[Conclusions]This method can realize rapid quantitative detection of PPR V antibody on site,and has high practical value and popularization value. 展开更多
关键词 Peste des Petits Ruminants n protein nH fusion protein Soluble expression and purification Rapid quantitative detection
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Establishment of Double-antigen Sandwich Time-resolved Fluorescence Immunoassay for Detection of Pest des Petits Ruminants Virus
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作者 Binglei CAO Zhongyuan GE +3 位作者 Qi YANG Hang SUN Yu SUN Xiaohui SONG 《Agricultural Biotechnology》 2024年第4期21-27,共7页
[Objectives]This study was conducted to explore rapid and large-scale screening and detection of peste des petits ruminants(PPR),so as to provide important technical means for prevention,control and purification of PP... [Objectives]This study was conducted to explore rapid and large-scale screening and detection of peste des petits ruminants(PPR),so as to provide important technical means for prevention,control and purification of PPR.[Methods]Soluble N protein and NH fusion protein were successfully obtained in an Escherichia coli expression system by optimizing E.coli codon and expression conditions.Furthermore,based on purified soluble N protein and NH fusion protein,a double-antigen sandwich time-resolved fluorescence immunoassay method for detection of peste des petits ruminants virus(PPRV)was established.[Results]The method has high sensitivity and specificity and can specifically detect the antibody against PPRV in sheep serum,and it has no cross reaction with other related diseases.The method was used to detect 292 clinical samples,and compared with French IDVET competition ELISA kit.The coincidence rates of positive samples and negative samples from the two kinds of test kits were 92.47%and 97.26%,respectively,and the overall coincidence rate was 94.86%.The intra-group and inter-group coefficients of variation in the repeatability test were less than 10%.[Conclusions]Compared with the traditional ELISA method,the double-antigen sandwich time-resolved fluorescence immunoassay for detection of PPRV has equivalent sensitivity and specificity,and simple and rapid operation,and thus high application and popularization value. 展开更多
关键词 Peste des petits ruminants n active protein nH fusion protein Soluble expression and purification Time-resolved fluorescence immunoassay
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Study on the optimal incident proton energy of ^(7)Li(p,n)^(7)Be neutron source for boron neutron capture therapy
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作者 Yi-Nan Zhu Zuo-Kang Lin +3 位作者 Hai-Yan Yu Ye Dai Zhi-Min Dai Xiao-Han Yu 《Nuclear Science and Techniques》 SCIE EI CAS CSCD 2024年第3期170-180,共11页
Boron neutron capture therapy(BNCT)is recognized as a precise binary targeted radiotherapy technique that effectively eliminates tumors through the^(10)B(n,α)^(7)Li nuclear reaction.Among various neutron sources,acce... Boron neutron capture therapy(BNCT)is recognized as a precise binary targeted radiotherapy technique that effectively eliminates tumors through the^(10)B(n,α)^(7)Li nuclear reaction.Among various neutron sources,accelerator-based sources have emerged as particularly promising for BNCT applications.The^(7)Li(p,n)^(7)Be reaction is highly regarded as a potential neutron source for BNCT,owing to its low threshold energy for the reaction,significant neutron yield,appropriate average neutron energy,and additional benefits.This study utilized Monte Carlo simulations to model the physical interactions within a lithium target subjected to proton bombardment,including neutron moderation by an MgF_(2)moderator and subsequent BNCT dose analysis using a Snyder head phantom.The study focused on calculating the yields of epithermal neutrons for various incident proton energies,finding an optimal energy at 2.7 MeV.Furthermore,the Snyder head phantom was employed in dose simulations to validate the effectiveness of this specific incident energy when utilizing a^(7)Li(p,n)^(7)Be neutron source for BNCT purposes. 展开更多
关键词 Boron neutron capture therapy ^(7)Li(p n)7Be neutron source Incident proton energy Monte Carlo simulation
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Boosting Fischer-Tropsch Synthesis via Tuning of N Dopants in TiO_(2)@CN-Supported Ru Catalysts 被引量:1
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作者 Xincheng Li Yunhao Liu +10 位作者 Dejian Zhao Shuaishuai Lyu Jingwei Ye Xiaoshen Li Peipei Wu Ye Tian Yingtian Zhang Tong Ding Song Song Qingpeng Cheng Xingang Li 《Transactions of Tianjin University》 EI CAS 2024年第1期90-102,共13页
Nitrogen(N)-doped carbon materials as metal catalyst supports have attracted signifi cant attention,but the eff ect of N dopants on catalytic performance remains unclear,especially for complex reaction processes such ... Nitrogen(N)-doped carbon materials as metal catalyst supports have attracted signifi cant attention,but the eff ect of N dopants on catalytic performance remains unclear,especially for complex reaction processes such as Fischer-Tropsch synthesis(FTS).Herein,we engineered ruthenium(Ru)FTS catalysts supported on N-doped carbon overlayers on TiO_(2)nanoparticles.By regulating the carbonization temperatures,we successfully controlled the types and contents of N dopants to identify their impacts on metal-support interactions(MSI).Our fi ndings revealed that N dopants establish a favorable surface environment for electron transfer from the support to the Ru species.Moreover,pyridinic N demonstrates the highest electron-donating ability,followed by pyrrolic N and graphitic N.In addition to realizing excellent catalytic stability,strengthening the interaction between Ru sites and N dopants increases the Ru^(0)/Ru^(δ+)ratios to enlarge the active site numbers and surface electron density of Ru species to enhance the strength of adsorbed CO.Consequently,it improves the catalyst’s overall performance,encompassing intrinsic and apparent activities,as well as its ability for carbon chain growth.Accordingly,the as-synthesized Ru/TiO_(2)@CN-700 catalyst with abundant pyridine N dopants exhibits a superhigh C_(5+)time yield of 219.4 mol CO/(mol Ru·h)and C_(5+)selectivity of 85.5%. 展开更多
关键词 Fischer-Tropsch synthesis n-doped carbon materials Ruthenium catalyst Pyridinic n Metal-n interaction
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Constructing long-cycling crystalline C_(3)N_(4)-based carbonaceous anodes for sodium-ion battery via N configuration control 被引量:2
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作者 Ying Wang Hongguan Li +5 位作者 Shuanlong Di Boyin Zhai Ping Niu Antonios Kelarakis Shulan Wang Li Li 《Carbon Energy》 SCIE EI CAS CSCD 2024年第1期159-171,共13页
Carbon nitrides with two-dimensional layered structures and high theoretical capacities are attractive as anode materials for sodium-ion batteries while their low crystallinity and insufficient structural stability st... Carbon nitrides with two-dimensional layered structures and high theoretical capacities are attractive as anode materials for sodium-ion batteries while their low crystallinity and insufficient structural stability strongly restrict their practical applications.Coupling carbon nitrides with conductive carbon may relieve these issues.However,little is known about the influence of nitrogen(N)configurations on the interactions between carbon and C_(3)N_(4),which is fundamentally critical for guiding the precise design of advanced C_(3)N_(4)-related electrodes.Herein,highly crystalline C_(3)N_(4)(poly(triazine imide),PTI)based all-carbon composites were developed by molten salt strategy.More importantly,the vital role of pyrrolic-N for enhancing charge transfer and boosting Na+storage of C_(3)N_(4)-based composites,which was confirmed by both theoretical and experimental evidence,was spot-highlighted for the first time.By elaborately controlling the salt composition,the composite with high pyrrolic-N and minimized graphitic-N content was obtained.Profiting from the formation of highly crystalline PTI and electrochemically favorable pyrrolic-N configurations,the composite delivered an unusual reverse growth and record-level cycling stability even after 5000 cycles along with high reversible capacity and outstanding full-cell capacity retention.This work broadens the energy storage applications of C_(3)N_(4) and provides new prospects for the design of advanced all-carbon electrodes. 展开更多
关键词 AnODE highly crystalline C_(3)n_(4) n configuration sodium-ion batteries ultra-long cyclic stability
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Clinical outcome and prognostic factors of T4N0M0 colon cancer after R0 resection:A retrospective study 被引量:1
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作者 Bang Liu Zhao-Xiong Zhang +3 位作者 Xin-Yang Nie Wei-Lin Sun Yong-Jia Yan Wei-Hua Fu 《World Journal of Gastrointestinal Oncology》 SCIE 2024年第5期1869-1877,共9页
BACKGROUND Paradoxically,patients with T4N0M0(stage II,no lymph node metastasis)colon cancer have a worse prognosis than those with T2N1-2M0(stage III).However,no previous report has addressed this issue.AIM To screen... BACKGROUND Paradoxically,patients with T4N0M0(stage II,no lymph node metastasis)colon cancer have a worse prognosis than those with T2N1-2M0(stage III).However,no previous report has addressed this issue.AIM To screen prognostic risk factors for T4N0M0 colon cancer and construct a prognostic nomogram model for these patients.METHODS Two hundred patients with T4N0M0 colon cancer were treated at Tianjin Medical University General Hospital between January 2017 and December 2021,of which 112 patients were assigned to the training cohort,and the remaining 88 patients were assigned to the validation cohort.Differences between the training and validation groups were analyzed.The training cohort was subjected to multi-variate analysis to select prognostic risk factors for T4N0M0 colon cancer,followed by the construction of a nomogram model.RESULTS The 3-year overall survival(OS)rates were 86.2%and 74.4%for the training and validation cohorts,respectively.Enterostomy(P=0.000),T stage(P=0.001),right hemicolon(P=0.025),irregular review(P=0.040),and carbohydrate antigen 199(CA199)(P=0.011)were independent risk factors of OS in patients with T4N0M0 colon cancer.A nomogram model with good concordance and accuracy was constructed.CONCLUSION Enterostomy,T stage,right hemicolon,irregular review,and CA199 were independent risk factors for OS in patients with T4N0M0 colon cancer.The nomogram model exhibited good agreement and accuracy. 展开更多
关键词 T4n0M0 colon cancer PROGnOSIS Multivariate analysis nOMOGRAM Colon cancer
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Optimized nitrogen application for maximizing yield and minimizing nitrogen loss in film mulching spring maize production on the Loess Plateau,China
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作者 Qilong Song Jie Zhang +3 位作者 Fangfang Zhang Yufang Shen Shanchao Yue Shiqing Li 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第5期1671-1684,共14页
Excessive use of N fertilizers(driven by high-yield goals)and its consequent environmental problems are becoming increasingly acute in agricultural systems.A 2-year field experiment was conducted to investigate the ef... Excessive use of N fertilizers(driven by high-yield goals)and its consequent environmental problems are becoming increasingly acute in agricultural systems.A 2-year field experiment was conducted to investigate the effects of three N application methods(application of solid granular urea once(OF)or twice(TF),application of solid granular urea mixed with controlled-release urea once(MF),and six N rates(0,60,120,180,240,and 300 kg N ha^(-1))on maize yield,economic benefits,N use efficiency,and soil N balance in the maize(Zea mays L.)film mulching system on the Loess Plateau,China.The grain yield and economic return of maize were significantly affected by the N rate and application method.Compared with the OF treatment,the MF treatment not only increased the maize yield(increased by 9.0-16.7%)but also improved the economic return(increased by 10.9-25.8%).The agronomic N use efficiency(NAE),N partial factor productivity(NPFP)and recovery N efficiency(NRE)were significantly improved by 19.3-66.7,9.0-16.7 and 40.2-71.5%,respectively,compared with the OF treatment.The economic optimal N rate(EONR)of the OF,TF,and MF was 145.6,147.2,and 144.9 kg ha^(-1) in 2019,and 206.4,186.4,and 146.0 kg ha^(-1) in 2020,respectively.The apparent soil N loss at EONR of the OF,TF,and MF were 97.1-100.5,78.5-79.3,and 50.5-68.1 kg ha^(-1),respectively.These results support MF as a one-time N application method for delivering high yields and economic benefits,with low N input requirements within film mulching spring maize system on the Loess Plateau. 展开更多
关键词 maize yield n management economic optimal n rate Loess Plateau
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猪流行性腹泻病毒SYBR Green I实时荧光定量PCR检测方法的建立 被引量:1
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作者 董苏洁 孔宁 +9 位作者 秦文珍 翟焕杰 翟雪滢 杨心雨 童武 郑浩 于海 童光志 李有文 单同领 《中国动物传染病学报》 CAS 北大核心 2024年第2期174-180,共7页
为了建立高效、灵敏的猪流行性腹泻病毒(PEDV)检测方法,本研究从GenBank数据库中获取PEDV N基因序列,扩增出PEDV N基因标准质粒,并在N基因的保守区域内设计了一对特异性荧光定量引物,成功建立了SYBR Green I实时荧光定量PCR检测方法。... 为了建立高效、灵敏的猪流行性腹泻病毒(PEDV)检测方法,本研究从GenBank数据库中获取PEDV N基因序列,扩增出PEDV N基因标准质粒,并在N基因的保守区域内设计了一对特异性荧光定量引物,成功建立了SYBR Green I实时荧光定量PCR检测方法。经过一系列试验表明,该检测方法线性关系良好,R^(2)值为0.99;特异性强,敏感性高,最低可检测至2.23 copies/μL,比普通PCR灵敏约100倍;重复性好,组内变异系数为0.25%~0.43%,组间变异系数为0.67%~0.97%;对于各地区96份临床样品检测出PEDV阳性率为25%。本研究建立的实时荧光定量PCR检测方法为PEDV的临床诊断、流行病学调查以及定量研究提供了有效的检测工具。 展开更多
关键词 猪流行性腹泻病毒 n基因 SYBR Green I 荧光定量PCR
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Combined application of organic fertilizer and chemical fertilizer alleviates the kernel position effect in summer maize by promoting post-silking nitrogen uptake and dry matter accumulation
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作者 Lichao Zhai Lihua Zhang +7 位作者 Yongzeng Cui Lifang Zhai Mengjing Zheng Yanrong Yao Jingting Zhang Wanbin Hou Liyong Wu Xiuling Jia 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第4期1179-1194,共16页
Adjusting agronomic measures to alleviate the kernel position effect in maize is important for ensuring high yields.In order to clarify whether the combined application of organic fertilizer and chemical fertilizer(CA... Adjusting agronomic measures to alleviate the kernel position effect in maize is important for ensuring high yields.In order to clarify whether the combined application of organic fertilizer and chemical fertilizer(CAOFCF)can alleviate the kernel position effect of summer maize,field experiments were conducted during the 2019 and 2020 growing seasons,and five treatments were assessed:CF,100%chemical fertilizer;OFCF1,15%organic fertilizer+85%chemical fertilizer;OFCF2,30%organic fertilizer+70%chemical fertilizer;OFCF3,45%organic fertilizer+55%chemical fertilizer;and OFCF4,60%organic fertilizer+40%chemical fertilizer.Compared with the CF treatment,the OFCF1 and OFCF2 treatments significantly alleviated the kernel position effect by increasing the weight ratio of inferior kernels to superior kernels and reducing the weight gap between the superior and inferior kernels.These effects were largely due to the improved filling and starch accumulation of inferior kernels.However,there were no obvious differences in the kernel position effect among plants treated with CF,OFCF3,or OFCF4 in most cases.Leaf area indexes,post-silking photosynthetic rates,and net assimilation rates were higher in plants treated with OFCF1 or OFCF2 than in those treated with CF,reflecting an enhanced photosynthetic capacity and improved postsilking dry matter accumulation(DMA)in the plants treated with OFCF1 or OFCF2.Compared with the CF treatment,the OFCF1 and OFCF2 treatments increased post-silking N uptake by 66.3 and 75.5%,respectively,which was the major factor driving post-silking photosynthetic capacity and DMA.Moreover,the increases in root DMA and zeatin riboside content observed following the OFCF1 and OFCF2 treatments resulted in reduced root senescence,which is associated with an increased post-silking N uptake.Analyses showed that post-silking N uptake,DMA,and grain yield in summer maize were negatively correlated with the kernel position effect.In conclusion,the combined application of 15-30%organic fertilizer and 70-85%chemical fertilizer alleviated the kernel position effect in summer maize by improving post-silking N uptake and DMA.These results provide new insights into how CAOFCF can be used to improve maize productivity. 展开更多
关键词 chemical fertilizer dry mater accumulation kernel position effect n uptake organic fertilizer
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High temperature treatment induced production of unreduced 2n pollen in Camellia oleifera
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作者 Hongda Deng Xiaoyu Zhang +5 位作者 Song Sheng Yuxun Huang Ziqi Ye Tongyue Zhang Xing Liu Zhiqiang Han 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第3期879-896,共18页
Unreduced gametes through chromosome doubling play a major role in the process of plant polyploidization.Our previous work confirmed that Camellia oleifera can produce natural 2n pollen,and it is possible to induce th... Unreduced gametes through chromosome doubling play a major role in the process of plant polyploidization.Our previous work confirmed that Camellia oleifera can produce natural 2n pollen,and it is possible to induce the 2n pollen formation by high temperature treatment.This study focused on the optimization of the 2n pollen induction technique and the mechanisms of high temperature-induced2n pollen formation in C.oleifera.We found that the optimal protocol for inducing 2n pollen via high temperature was to perform 45℃with4 h at the prophaseⅠstage of the pollen mother cells(PMCs).Meanwhile,high temperature significantly decreased the yield and fertility of2n pollen.Through the observation of meiosis,abnormal chromosome and cytological behaviour was discovered under high-temperature treatment,and we confirmed that the formation of 2n pollen is caused by abnormal cell plate.Based on weighted gene co-expression network analysis,fifteen hub genes related to cell cycle control were identified.After male flower buds were exposed to heat shock,polygalacturonase gene(CoPGX3)was significantly upregulated.We inferred that high temperature causes the CoPGX3 gene to be overexpressed and that CoPGX3 is redistributed into the cytosol where it degrades cytoplasmic pectin,which leads to an abnormal cell plate.Furthermore,abnormal cytokinesis resulted in the formation of dyads and triads,and PMCs divided to produce 2n pollen.Our findings provide new insights into the mechanism of 2n pollen induced by high temperature in a woody plant and lay a foundation for further ploidy breeding of C.oleifera. 展开更多
关键词 Camellia oleifera Pollen mother cells(PMCs) High temperature MEIOSIS 2n pollen CoPGX3
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Effects of long-term partial substitution of inorganic fertilizer with pig manure and/or straw on nitrogen fractions and microbiological properties in greenhouse vegetable soils
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作者 Shuo Yuan Ruonan Li +5 位作者 Yinjie Zhang Hao'an Luan Jiwei Tang Liying Wang Hongjie Ji Shaowen Huang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第6期2083-2098,共16页
Partial substitution of inorganic fertilizers with organic amendments is an important agricultural management practice.An 11-year field experiment(22 cropping periods)was carried out to analyze the impacts of differen... Partial substitution of inorganic fertilizers with organic amendments is an important agricultural management practice.An 11-year field experiment(22 cropping periods)was carried out to analyze the impacts of different partial substitution treatments on crop yields and the transformation of nitrogen fractions in greenhouse vegetable soil.Four treatments with equal N,P_(2)O_(5),and K_(2)O inputs were selected,including complete inorganic fertilizer N(CN),50%inorganic fertilizer N plus 50%pig manure N(CPN),50%inorganic fertilizer N plus 25%pig manure N and 25%corn straw N(CPSN),and 50%inorganic fertilizer N plus 50%corn straw N(CSN).Organic substitution treatments tended to increase crop yields since the 6th cropping period compared to the CN treatment.From the 8th to the 22nd cropping periods,the highest yields were observed in the CPSN treatment where yields were 7.5-11.1%greater than in CN treatment.After 11-year fertilization,compared to CN,organic substitution treatments significantly increased the concentrations of NO_(3)^(-)-N,NH_(4)^(+)-N,acid hydrolysis ammonium-N(AHAN),amino acid-N(AAN),amino sugar-N(ASN),and acid hydrolysis unknown-N(AHUN)in soil by 45.0-69.4,32.8-58.1,49.3-66.6,62.0-69.5,34.5-100.3,and 109.2-172.9%,respectively.Redundancy analysis indicated that soil C/N and OC concentration significantly affected the distribution of N fractions.The highest concentrations of NO_(3)^(-)-N,AHAN,AAN,AHUN were found in the CPSN treatment.Organic substitution treatments increased the activities ofβ-glucosidase,β-cellobiosidase,N-acetyl-glucosamidase,L-aminopeptidase,and phosphatase in the soil.Organic substitution treatments reduced vector length and increased vector angle,indicating alleviation of constraints of C and N on soil microorganisms.Organic substitution treatments increased the total concentrations of phospholipid fatty acids(PLFAs)in the soil by 109.9-205.3%,and increased the relative abundance of G^(+)bacteria and fungi taxa,but decreased the relative abundance of G-bacteria,total bacteria,and actinomycetes.Overall,long-term organic substitution management increased soil OC concentration,C/N,and the microbial population,the latter in turn positively influenced soil enzyme activity.Enhanced microorganism numbers and enzyme activity enhanced soil N sequestration by transforming inorganic N to acid hydrolysis-N(AHN),and enhanced soil N supply capacity by activating non-acid hydrolysis-N(NAHN)to AHN,thus improving vegetable yield.Application of inorganic fertilizer,manure,and straw was a more effective fertilization model for achieving sustainable greenhouse vegetable production than application of inorganic fertilizer alone. 展开更多
关键词 organic substitution management greenhouse vegetable production n fractions microbial community composition extracellular enzyme activity enzyme stoichiometry coefficients
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基于Swin Transformer的遥感图像超分辨率重建
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作者 孔锐 冉友红 《计算机系统应用》 2024年第9期85-94,共10页
由于遥感图像中的物体具有不确定性,同时不同图像之间的特征信息差异较大,导致现有超分辨率方法重建效果差,因此本文提出一种结合Swin Transformer和N-gram模型的NG-MAT模型来实现遥感图像超分辨率.首先,在原始Transformer计算自注意力... 由于遥感图像中的物体具有不确定性,同时不同图像之间的特征信息差异较大,导致现有超分辨率方法重建效果差,因此本文提出一种结合Swin Transformer和N-gram模型的NG-MAT模型来实现遥感图像超分辨率.首先,在原始Transformer计算自注意力的分支上并联多注意力模块,用于提取全局特征信息来激活更多像素.其次,将自然语言处理领域的N-gram模型应用到图像处理领域,用三元N-gram模型来加强窗口之间的信息交互.本文提出的方法在所选取的数据集上,峰值信噪比在放大因子为2、3、4时达到了34.68 dB、31.03 dB、28.99 dB,结构相似度在放大因子为2、3、4时达到了0.9266、0.8444、0.7734,实验结果表明,本文提出的方法各个指标都优于其他同类方法. 展开更多
关键词 Swin Transformer 超分辨率 n-GRAM 遥感图像
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Kármán vortex street in a spin–orbit-coupled Bose–Einstein condensate with PT symmetry
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作者 邵凯花 席保龙 +5 位作者 席忠红 涂朴 王青青 马金萍 赵茜 石玉仁 《Chinese Physics B》 SCIE EI CAS CSCD 2024年第6期289-296,共8页
The dynamics of spin–orbit-coupled Bose–Einstein condensate with parity-time symmetry through a moving obstacle potential is simulated numerically. In the miscible two-component condensate, the formation of the K... The dynamics of spin–orbit-coupled Bose–Einstein condensate with parity-time symmetry through a moving obstacle potential is simulated numerically. In the miscible two-component condensate, the formation of the Kármán vortex street is observed in one component, while ‘the half-quantum vortex street' is observed in the other component. Other patterns of vortex shedding, such as oblique vortex dipoles, V-shaped vortex pairs, irregular turbulence, and combined modes of various wakes, can also be found. The ratio of inter-vortex spacing in one row to the distance between vortex rows is approximately0.18, which is less than the stability condition 0.28 of classical fluid. The drag force acting on the obstacle potential is simulated. The parametric regions of Kármán vortex street and other vortex patterns are calculated. The range of Kármán vortex street is surrounded by the region of combined modes. In addition, spin–orbit coupling disrupts the symmetry of the system and the gain-loss affects the local particle distribution of the system, which leads to the local symmetry breaking of the system, and finally influences the stability of the Kármán vortex street. Finally, we propose an experimental protocol to realize the Kármán vortex street in a system. 展开更多
关键词 Kármán vortex street Bose–Einstein condensate spin–orbit-coupled parity-time symmetry
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A Novel Foreign Object Detection Method in Transmission Lines Based on Improved YOLOv8n
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作者 Yakui Liu Xing Jiang +4 位作者 Ruikang Xu Yihao Cui Chenhui Yu Jingqi Yang Jishuai Zhou 《Computers, Materials & Continua》 SCIE EI 2024年第4期1263-1279,共17页
The rapid pace of urban development has resulted in the widespread presence of construction equipment andincreasingly complex conditions in transmission corridors. These conditions pose a serious threat to the safeope... The rapid pace of urban development has resulted in the widespread presence of construction equipment andincreasingly complex conditions in transmission corridors. These conditions pose a serious threat to the safeoperation of the power grid.Machine vision technology, particularly object recognition technology, has beenwidelyemployed to identify foreign objects in transmission line images. Despite its wide application, the technique faceslimitations due to the complex environmental background and other auxiliary factors. To address these challenges,this study introduces an improved YOLOv8n. The traditional stepwise convolution and pooling layers are replacedwith a spatial-depth convolution (SPD-Conv) module, aiming to improve the algorithm’s efficacy in recognizinglow-resolution and small-size objects. The algorithm’s feature extraction network is improved by using a LargeSelective Kernel (LSK) attention mechanism, which enhances the ability to extract relevant features. Additionally,the SIoU Loss function is used instead of the Complete Intersection over Union (CIoU) Loss to facilitate fasterconvergence of the algorithm. Through experimental verification, the improved YOLOv8n model achieves adetection accuracy of 88.8% on the test set. The recognition accuracy of cranes is improved by 2.9%, which isa significant enhancement compared to the unimproved algorithm. This improvement effectively enhances theaccuracy of recognizing foreign objects on transmission lines and proves the effectiveness of the new algorithm. 展开更多
关键词 YOLOv8n data enhancement attention mechanism SPD-Conv Smoothed Intersection over Union(SIoU)Loss
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Nano-multilayered ZrN‒Ag/Mo‒S‒N film design for stable anti-frictional performance at a wide range of temperatures
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作者 Hongbo JU Jing LUAN +3 位作者 Junhua XU Albano CAVALEIRO Manuel EVARISTO Filipe FERNANDES 《Friction》 SCIE EI CAS CSCD 2024年第12期2826-2837,共12页
A multilayer film,composed by ZrN‒Ag(20 nm)and Mo‒S‒N(10 nm)layers,combining the intrinsic lubricant characteristics of each layer was deposited using DC magnetron sputtering system,to promote lubrication in a wide-ra... A multilayer film,composed by ZrN‒Ag(20 nm)and Mo‒S‒N(10 nm)layers,combining the intrinsic lubricant characteristics of each layer was deposited using DC magnetron sputtering system,to promote lubrication in a wide-range of temperatures.The results showed that the ZrN‒Ag/Mo‒S‒N multilayer film exhibited a sharp interface between the different layers.A face-centered cubic(fcc)dual-phases of ZrN and Ag co-existed in the ZrN‒Ag layers,whilst the Mo‒S‒N layers displayed a mixture of hexagonal close-packed MoS_(2)(hcp-MoS_(2))nano-particles and an amorphous phase.The multilayer film exhibited excellent room temperature(RT)triblogical behavior,as compared to the individual monolayer film,due to the combination of a relative high hardness with the low friction properties of both layers.The reorientation of MoS_(2)parallel to the sliding direction also contributed to the enhanced anti-frictional performance at RT.At 400℃,the reorientation of MoS_(2)as well as the formation of MoO_(3)phase were responsible for the lubrication,whilst the hard t-ZrO_(2)phase promoted abrasion and,consequently,led to increasing wear rate.At 600℃,the Ag_(2)MoO_(4)double-metal oxide was the responsible for the low friction and wear-resistance;furthermore,the observed transformation from t-ZrO_(2)to m-ZrO_(2),could also have contributed to the better tribological performance. 展开更多
关键词 DC magnetron sputtering Zrn‒Ag/Mo‒S‒n multilayer film tribological properties tribo-phases
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