Effects of Nitrogen Application on Chlorophyll Fluorescence Parameters and Leaf Gas Exchange in Naked Oat

Naked oat（Avena nuda L.） was originated from China,where soil nitrogen（N） is low availability.The responses of chlorophyll（Chl.） fluorescence parameters and leaf gas exchange to N application were analysed in this study.After the N application rate ranged from 60 to 120 kg ha-1,variable fluorescence（F v）,the maximal fluorescence（F m）,the maximal photochemical efficiency（F v /F m）,quantum yield（Φ PS II） of the photosynthetic system II（PS II）,electron transport rate（ETR）,and photochemical quenching coefficient（qP） increased with N application level,however,non-photochemical quenching coefficient（qN） decreased.Moreover,there was no difference in initial fluorescence（F o） with further more N enhancement.The maximum net photosynthetic rate（P max）,apparent dark respiration rate（R d） and light saturation point（LSP） were improved with 40-56 kg N ha-1as basal fertilizer and 24-40 kg N ha-1as top dressing fertilizer applied at jointing stage.Initial quantum yield（α） was decreased with 24 kg N ha-1as basal fertilizer and 56 kg N ha-1as top dressing fertilizer.Flag-leaf net photosynthetic rate（P n） was significantly enhanced at the jointing and heading stages with 40-56 kg N ha-1as basal fertilizer; in addition,increased at grain filling stage of naked oat with 40-56 kg N ha-1as top dressing fertilizer.90 kg N ha-1（50-70% as basal fertilizer and 30-50% as top dressing fertilizer） application is recommended to alleviate photodamage of photosystem and improve the photosynthetic rate in naked oat.

Ethylene accelerates maize leaf senescence in response to nitrogen deficiency by regulating chlorophyll metabolism and autophagy

Leaf senescence is an orderly and highly coordinated process,and finely regulated by ethylene and nitrogen(N),ultimately affecting grain yield and nitrogen-use efficiency(NUE).However,the underlying regulatory mechanisms on the crosstalk between ethylene-and N-regulated leaf senescence remain a mystery in maize.In this study,ethylene biosynthesis gene ZmACS7 overexpressing(OE-ZmACS7)plants were used to study the role of ethylene regulating leaf senescence in response to N deficiency,and they exhibited the premature leaf senescence accompanied by increased ethylene release,decreased chlorophyll content and F_v/F_m ratio,and accelerated chloroplast degradation.Then,we investigated the dynamics changes of transcriptome reprogramming underlying ethylene-accelerated leaf senescence in response to N deficiency.The differentially expressed genes(DEGs)involved in chlorophyll biosynthesis were significantly down-regulated,while DEGs involved in chlorophyll degradation and autophagy processes were significantly up-regulated,especially in OE-ZmACS7 plants in response to N deficiency.A gene regulatory network(GRN)was predicted during ethylene-accelerated leaf senescence in response to N deficiency.Three transcription factors(TFs)ZmHSF4,Zmb HLH106,and ZmEREB147 were identified as the key regulatory genes,which targeted chlorophyll biosynthesis gene ZmLES22,chlorophyll degradation gene ZmNYC1,and autophagy-related gene ZmATG5,respectively.Furthermore,ethylene signaling key genes might be located upstream of these TFs,generating the signaling cascade networks during ethylene-accelerated leaf senescence in response to N deficiency.Collectively,these findings improve our molecular knowledge of ethylene-accelerated maize leaf senescence in response to N deficiency,which is promising to improve NUE by manipulating the progress of leaf senescence in maize.

Decreased panicle N application alleviates the negative effects of shading on rice grain yield and grain quality

Light deficiency is a growing abiotic stress in rice production.However,few studies focus on shading effects on grain yield and quality of rice in East China.It is also essential to investigate proper nitrogen(N)application strategies that can effectively alleviate the negative impacts of light deficiency on grain yield and quality in rice.A two-year field experiment was conducted to explore the effects of shading(non-shading and shading from heading to maturity)and panicle N application(NDP,decreased panicle N rate;NMP,medium panicle N rate;NIP,increased panicle N rate)treatments on rice yield-and quality-related characteristics.Compared with non-shading,shading resulted in a 9.5-14.8%yield loss(P<0.05),mainly due to lower filled-grain percentage and grain weight.NMP and NIP had higher(P<0.05)grain yield than NDP under non-shading,and no significant difference was observed in rice grain yield among NDP,NMP,and NIP under shading.Compared with NMP and NIP,NDP achieved less yield loss under shading because of the increased filled-grain percentage and grain weight.Shading reduced leaf photosynthetic rate after heading,as well as shoot biomass weight at maturity,shoot biomass accumulation from heading to maturity,and nonstructural carbohydrate(NSC)content in the stem at maturity(P<0.05).The harvest index and NSC remobilization reserve of NDP were increased under shading.Shading decreased(P<0.05)percentages of brown rice,milled rice,head rice,and amylose content while increasing(P<0.05)chalky rice percentage,chalky area,chalky degree,and grain protein.NMP demonstrated a better milling quality under non-shading,while NDP demonstrated under shading.NDP exhibited both lower chalky rice percentage,chalky area,and chalky degree under non-shading and shading,compared with NMP and NIP.NDP under shading decreased amylose content and breakdown but increased grain protein content and setback,contributing to similar overall palatability to non-shading.Our results suggested severe grain yield and quality penalty of rice when subjected to shading after heading.NDP improved NSC remobilization,harvest index,and sink-filling efficiency and alleviated yield loss under shading.Besides,NDP would maintain rice’s milling,appearance,and cooking and eating qualities under shading.Proper N management with a decreased panicle N rate could be adopted to mitigate the negative effects of shading on rice grain yield and quality.

Differing responses of root morphology and physiology to nitrogen application rates and their relationships with grain yield in rice

Root morphology and physiology influence aboveground growth and yield formation in rice.However,root morphological and physiological differences among rice varieties with differing nitrogen(N)sensitivities and their relationship with grain yield are still unclear.In this study,rice varieties differing in N sensitivity over many years of experiments were used.A field experiment with multiple N rates(0,90,180,270,and 360 kg ha^(-1))was conducted to elucidate the effects of N application on root morphology,root physiology,and grain yield.A pot experiment with root excision and exogenous application of 6-benzyladenine(6-BA)at heading stage was used to further verify the above effects.The findings revealed that(1)under the same N application rate,N-insensitive varieties(NIV)had relatively large root biomass(root dry weight,length,and number).Grain yield was associated with root biomass in NIV.The oxidation activity and zeatin(Z)+zeatin riboside(ZR)contents in roots obviously and positively correlated with grain yield in N-sensitive varieties(NSV),and accounted for its higher grain yield than that of NIV at lower N application rates(90 and 180 kg ha^(-1)).(2)The root dry weight required for equal grain yield of NIV was greater than that of NSV.Excision of 1/10 and 1/8 of roots at heading stage had no discernible effect on the yield of Liangyoupeijiu(NIV),and it significantly reduced yield by 11.5%and 21.3%in Tianyouhuazhan(NSV),respectively,compared to the treatment without root excision.The decrease of filled kernels and grain weight after root excision was the primary cause for the yield reduction.Root excision and exogenous 6-BA application after root excision had little influence on the root activity of NIV.The oxidation activity and Z+ZR contents in roots of NSV decreased under root excision,and the increase in the proportion of excised roots aggravated these effects.The application of exogenous 6-BA increased the root activity of NSV and increased filled kernels and grain weight,thereby reducing yield loss after root excision.Thus,the root biomass of NIV was large,and there may be a phenomenon of"root growth redundancy."Vigorous root activity was an essential feature of NSV.Selecting rice varieties with high root activity or increasing root activity by cultivation measures could lead to higher grain yield under lower N application rates.

Nutrient Application Model Affects the Contents of Chlorophyll and Carotenoid in Functional Leaves of Early Rice

The objective of this study was to investigate the effects of different nutri-ent application models on the contents of chlorophyl and carotenoid in the functional leaves of early rice. Using rice cultivar Xiangzaoxian45 as experimental materials, the experiment was performed by designing 6 treatments, i.e., T1 （fertilization without nitrogen）, T2（local conventional fertilization）, T3（fertilization for high yield and high effi-ciency）, T4 （fertilization for super high yield）, T5 （fertilization application for super high yield and high efficiency A） and T6 （fertilization application for super high yield and high efficiency B） in two experimental plots Yiyang and Xiangyin. The results showed that T3 respectively increased the contents of chlorophyl and carotenoid at fil ing stage by 29.27%, 38.20% and 13.16%, 30.12% in Yiyang and Xiangyin, as wel as yield of early rice by 4.20%, 4.80% to T2 on the condition of saving 20% ni-trogen fertilizer. Additional y, T5 and T6 on the condition of saving 16.7% nitrogen fertilizer by T4 increased the contents of chlorophyl and carotenoid of fil ing stage by 53.91%, 53.73% and 35.95%, 37.47% in Yiyang and Xiangyin, as wel as yield of early rice by 16.60%, 18.75% to T2 in Yiyang; increased the contents of chlorophyl and carotenoid at fil ing stage by 57.82%, 56.80% and 54.88%, 57.03% in Yiyang and Xiangyin, as wel as yield of early rice 10.10%, 6.75% to T2 in Xiangyin. More-over, there was a significant correlation or an extremely significant correlation be-tween yield and the contents of chlorophyl and carotenoid at different soil fertility level （P〈0.05 or P〈0.01）. Therefore, nutrient application plays an important role in the contents of chlorophyl and carotenoid in the functional leaves of early rice.

Effects of Water and Nitrogen Application on Photosynthetic Characteristics of Flag Leaves and Grain Yield of Wheat

[Objective] The aim was to provide a scientific and rational water,nitrogen model for achieving high yield,high quality of wheat and water and fertilizer saving.[Method] Under the field conditions,the effects of irrigation frequencies and N application times on net photosynthetic rate（Pn）,chlorophyll fluorescence parameters of flag leaf and yield of wheat was studied.[Result] The differences on Pn,chlorophyll fluorescence parameters Fv/Fo,Fv/Fm,qP,qN of flag leaves and yield of wheat among different irrigation times had reached a significant level,and the difference between treatment without irrigation and irrigation treatments were relatively large;the difference on Pn,Fv/Fo,Fv/Fm,qP and qN between treatments of basal application of N fertilizer and N application（40%）in returning green stage was not significant,however,the photosynthetic parameters and yield of two treatments were obviously lower than that of N application（40%）in jointing stage or booting stage.[Conclusion] The effects of irrigation frequencies and N application times showed significant interaction effects on photosynthetic characteristics of wheat flag leaves and yield,in which W2N4 treatment combination was best.

Distribution and accumulation of zinc and nitrogen in wheat grain pearling fractions in response to foliar zinc and soil nitrogen applications

Increasing zinc(Zn)concentration in wheat grain is important to minimize human dietary Zn deficiency.This study aimed to investigate the effect of foliar Zn and soil nitrogen(N)applications on the accumulation and distribution of N and Zn in grain pearling fractions,N remobilization,and the relationships between nutrient concentration in the vegetative tissues and grain or its fractions in two cropping years in the North China Plain.The results showed a progressive decrease in N and Zn concentrations from the outer to the inner parts of grain,with most of the accumulation in the core endosperm.Foliar Zn application significantly increased N concentration in the pericarp,and soil N application increased N concentration in each grain fraction.Both treatments significantly increased core endosperm Zn concentration.Foliar Zn had no effect on grain N and Zn distribution.Soil N application made N concentrated in the aleurone,promoted Zn translocation to the core endosperm and also increased N remobilization and its efficiency from the shoot to the grain,but no improved contribution to grain was found.N concentration in grain and its fractions were positively correlated with N in vegetative organs at anthesis and maturity,while positive correlations were obtained between N concentration in the pericarp and progressive central area of the endosperm and Zn concentration in the core endosperm.Thus,foliar Zn and soil N applications effectively increased yield and N and Zn concentrations in the wheat grain,particularly in the endosperm,and could be promising strategies to address Zn deficiency.

Effects of Postponing N Application on Metabolism,Absorption and Utilization of Nitrogen of Summer Maize in SuperHigh Yield Region

[Objective] The aim was to explore effects of application postponing of N fertilizer and the mechanism of yield increase in order to provide references for N fertilizer application in a rational way. [Method] In a super-high yielded region of summer maize, field experiment was conducted to research effects of N fertilizer postponing on key enzymes of N metabolism, yield of maize and N fertilizer use. [Result] After application of N fertilizer was postponed, NR, SPS and GS activities of ear-leaf of summer maize increased by 11.99%-34.87%, 8.25%-10.64% and 10.00%- 16.81% on the 28^th d of silking; content of soluble sugar in leaves enhanced signifi- cantly and accumulated nitrogen increased by 5.00%-9.74% in mature stage. The postponing fertilization of ＂30% of fertilizer in seedling stage＋30% of fertilizer in flare- opening stage＋40% of fertilizer in silking stage meets N demands of summer maize in late growth period. Compared with conventional fertilization, the maize yield, agro- nomic efficiency and use of N fertilizer all improved by 5.05%, 1.75 kg/kg and 6.87%, respectively, after application postponed. [Conclusion] Application postponing of N fertilizer maintains activity of NR, GS and SPS higher and coordinates metabolism of C and N in late growth period, to further improve yield of maize.

Dependence of Wheat and Rice Respiration on Tissue Nitrogen and the Corresponding Net Carbon Fixation Efficiency Under Different Rates of Nitrogen Application

To quantitatively address the role of tissue N in crop respiration under various agricultural practices, and to consequently evaluate the impact of synthetic fertilizer N application on biomass production and respiration, and hence net carbon fixation efficiency （Encf）, pot and field experiments were carried out for an annual rotation of a rice-wheat cropping system from 2001 to 2003. The treatments of the pot experiments included fertilizer N application, sowing date and planting density. Different rates of N application were tested in the field experiments. Static opaque chambers were used for sampling the gas. The respiration as CO2 emission was detected by a gas chromatograph. A successive biomass clipping method was employed to determine the crop autotrophic respiration coefficient （Ra）. Results from the pot experiments revealed a linear relationship between Ra and tissue N content as Ra = 4.74N-1.45 （R^2= 0.85, P 〈 0.001）. Measurements and calculations from the field experiments indicated that fertilizer N application promoted not only biomass production but also increased the respiration of crops. A further investigation showed that the increase of carbon loss in terms of respiration owing to fertilizer N application exceeded that of net carbon gain in terms of aboveground biomass when fertilizer N was applied over a certain rate. Consequently, the Encf declined as the N application rate increased.

Effect of Postponing Nitrogen Application on Growth and Yield of Different Wheat Cultivars

[Objective] The aim was to study whether there are differences in top- dressing period for wheat cultivars. [Method] With three representative wheat culti- vats selected, effects of different N topdressing period on wheat tiller dynamics and yield components were studied. [Result] Appropriately postponing N topdressing time improved the yield of wheat. Appropriate N topdressing time tended to be volatile upon wheat cultivar. Jointing stage was appropriate for topdresSing for most wheat cultivars such as Jimai 22 and Linmai No. 4 and flowering stage was appropriate for the cultivar featured by early-senescence such as LN66. [Conclusion]It is neces- sary to select the optimal topdressing time according to wheat characteristics.

Effects of N Application Rates and NO_3-N to NH_4-N Ratios on Yield and Quality of Flue-cured Tobacco Planted in Black Soil

A field experiment about effects of nitrogen application rates and different NO3-N to NH4-N ratios on agronomic, chemical and biological characteristics as well as yield and quality of flue-cured tobacco grown in a black soil was conducted from 2004 to 2005 in Heilongjiang Province. The results showed that the nitrogen application rates at 45 kg·hm^-2 with the ratio of 75% NO3-N to 25% NH4-N resulted in the highest potassium and reducing sugar contents in the flue-cured tobacco leaving with the highest quality grade and value. It is recommended that this ni- trogen application rate and NO3-N to NH4-N ratio should be widely applied on flue-cured tobacco grown in the black soil in Heilongjiang Province.

Effects of Nitrogen Application Rate and Seeding Density on Plant Growth and Seed Yield of Direct-seeding Rape

[Objectives] This study was conducted to explore the effects of different N application rates and densities on the growth and development of direct-seeding rape as well as on its yield.[Methods] A field experiment was carried out by setting five N fertilizer treatments and three density levels.[Results] Increasing seeding density and N application rate could improve the seed yield of rape. The suitable N application rate for the three densities were calculated using the fitted fertilizer efficiency models, respectively, to be 186.77, 221.35 and 236.14 kg/hm^2, at which the yields were the highest. The results showed that in this area, the seeding density of direct-seeding rape could be selected in the range of 6.0×10^5-7.5×10^5 plants/hm^2, and the suitable N application rate could be in the range of 221.35-236.14 kg/hm^2.[Conclusions] This study provides a theoretical basis for the maximization of spatial resource and efficient utilization of fertilizer.

Short Response of Spring Wheat to Tillage, Residue Management and Split Nitrogen Application in a Rice-Wheat System

A ifeld experiment was conducted to study the impact of tillage, crop residue management and nitrogen （N） splitting on spring wheat （Triticum aestivum L.） yield over 2 yr （2010-2012） in a rice （Oryza sativa L.）-wheat system in northwestern Pakistan. The experiment was conducted as split plot arranged in randomized complete blocks design with three replications. Treatments comprised six tillage and residue managements：zero tillage straw retained （ZTsr）, zero tillage straw burnt （ZTsb）, reduced tillage straw incorporated （RTsi）, reduced tillage straw burnt （RTsb）, conventional tillage straw incorporated （CTsi）, and conventional tillage straw burnt （CTsb） as main plots and N （200 kg ha-1） was applied as split form viz., control （no nitrogen＆no splitting, N0S0）;2 splits of total N, half at sowing and half at the 1st irrigation （i.e., 20 d after sowing （DAS）） （NS1）;3 splits of total N, 1/3 at sowing, 1/3 at the 1st irrigation, and 1/3 at the 2nd irrigation （NS2）;4 splits of total N, 1/4 at sowing, 1/4 at the 1st irrigation, 1/4 at the 2nd irrigation （45 DAS）, and 1/4 at the 3rd irrigation （70 DAS） （NS3）;and 4 splits of total N, 1/4 at the 1st irrigation, 1/4 at the 2nd irrigation, 1/4 at the 3rd irrigation, and 1/4 at the 4th irrigation （95DAS） （NS4） as sub plots. The results showed that the most pikes m-2, grains/spike, 1 000-grain weight, grain yield, and N use efifciency （NUE） were obtained at zero tillage, straw retained and 4 splits application of total N （i.e., at sowing 20, 45 and 70 d after sowing）. The results indicated that ZTsr with application of 200 kg N ha-1 in 4 equal splits viz. at sowing 20, 45 and 70 d after sowing is an appropriate strategy that enhanced wheat yield （7 436-7 634 kg ha-1） and N efifciency （28.6-29.5 kg kg-1） in rice-wheat system.

Effects of Late Nitrogen Application on Nitrogen Translocation and Protein Fractions of Wheat Genotypes Differing in Protein Content

In order to investigate the effeits of late nitrogen application on nitrogen translocation and protein fractions, three genotypes differing in protein content were studied in pot experiments at low and high fertility regimes with late foliar nitrogen application. At high fertility, late nitrogen application increased N translocation and improved N translocation efficiency greatly, however, cultivar differences were found at low fertility and late nitrogen application increased both leaf and chaff N translocation, and increased culm N translocation only at high fertility. Relative contributions of vegetative components to N translocation efficiency were altered by late nitrogen application. Albumin and gliadin contents at maturity were decreased by late nitrogen application for all cultivars used, and cultivar variations for globulins were also observed. Xin Kehan No. 9, the high yielding, low grain protein content cultivar remained no change for glutenin content to late nitrogen application, Dongnong 7742, the high yielding, high grain protein content, decreased slightly, and Roblin, high grain protein but low yielding cultivar decreased only at hihg fertility. Residual protein contents were significantly increased by late nitrogen application for all cultivars. It was concluded that nitrogen applied at later stage could be used efficiently noly at high fertility, and most of the N translocated were used for the synthesis of residual proteins.

Effects of Different Nitrogen Applications on Soil Physical, Chemical Properties and Yield in Maize (<i>Zea mays</i>L.)

Application of nitrogen (N) fertilizer is one of the most important approaches on improving maize grain yield. However, as is known to all, overuse N fertilizer not only leads to decline of N use efficiency and maize yield, but also leads to potential risk to environment pollution. This experiment was conducted to determine the effects of N fertilizer applications with nine different treatments on soil physical-chemical characters and maize grain yield using hybrid variety Zhengdan 958 in 2011 and 2012. Results indicated that the soil bulk densities of T2 (CK) and T1 were the lowest compared to other treatments in 2011 and 2012, respectively, whereas the soil bulk density of T5 in 2011 and T3 in 2012 were higher than other treatments. The soil porosity and field capacity of T5 in 2011 and T3 in 2012 were lower than other treatments, but those of CK in 2011 and T1 in 2012 were higher than other treatments. The pH values of T3 to T7 were lower than other treatments. These results indicated that the soil bulk densities were increased, whereas the soil porosity, field capacity and values pH were decreased by N application at different stages. N application could increase the N contents of leaf and stem, whereas less or excess N application should not significant improve maize yield. Although the soil organic matter and total N contents of T3 were the highest in both 2011 and 2012, the yield of T4 is the highest in both 2011 and 2012. The application amount, period and times of N fertilizer were important to maize yield.

STUDY ON APPLICATION OF AERATION BIOLOGICAL FLUID TANK TECHNOLGY IN NH_4^+-N WASTE WATER TREATMENT

This paper introduces an application of "Aeration biological fluid tank" technology (ABFT) for the treatment of waste water containing NH + 4 N and high concentrated organic chemicals. Highlights were focused on the effects of dissolved oxygen, pH, temperature and retention time on waste water biological treatment in order to find out a new approach in treatment of waste water containing high concentrated NH + 4 N.

Effect of Silicon Amendment on Growth and Nitrogen Status of Common Landscaping Plants

Agriculture and natural vegetations in South Florida face with significant environmental threats such as heat and saltwater intrusion. This study aimed to investigate how silicon application could improve growth parameters and plant health of landscaping plants under extreme temperatures, influenced by global climate changes. Cocoplum (Chrysobalanus icaco), cabbage palm (Sabal palmetto), satinleaf (Chrysophyllum oliviforme), and wild coffee (Psychotria nervosa) plants received an initial slow-release fertilizer of 15 g/pot with an 8N-3P-9K composition. Silicon was applied as a 1% silicic acid solution, with concentrations ranging from 0 g/pot to 6 g/pot of 7.5 L. Evaluations were carried out every 30 days, continuing until 180 days after the treatment was completed. Phenotypic traits, including leaf count and plant height, were assessed alongside measurements from handheld optical non-destructive sensors. These measurements included the normalized difference vegetation index (NDVI), SPAD-502, and atLEAF chlorophyll meters. Application of 4 g/pot and 6 g/pot of silicon significantly improved NDVI values (0.78). Conversely, cocoplum plants exhibited greater plant height (79.6) at 0 g/pot silicon compared to other treatments. In wild coffee samplings, the control group showed the highest plant height and SPAD readings (93.49) compared to other treatments. Interestingly, the control treatment also demonstrated a superior atLEAF value as compared to other treatments, while the tallest samplings were observed with 6 g/pot of silicon (62.82) in cabbage palm plants. The findings indicate that silicon application positively influenced plant growth, particularly evident in cabbage palms. However, cocoplum and wild coffee exhibited a negative correlation between plant height and silicon concentrations.

200 N推力装置上肼类分解催化剂的应用性能

研究制备了一种单组元姿轨控发动机用肼类分解催化剂,采用200 N推力装置对其应用性能进行了研究和考察。在催化剂物化性能指标满足应用要求的基础上,利用200 N推力装置通过力学环境试验考察了催化剂力学环境适应性,通过双-10℃低温冷启动、低温稳态及脉冲、正负拉偏以及脉冲寿命热试考核程序考察了自制催化剂的低温冷启动性能、低温稳态性能、常温正负拉偏稳态性能以及脉冲寿命性能。结果表明:该催化剂肼催化分解效率在99%以上,机械强度高且力学环境适应性良好,具有较高的肼催化分解活性和稳定性,能够在200 N推力装置上于双-10℃正常启动,顺利完成了低温稳态、常温正负拉偏和脉冲寿命等考核程序,发动机室压平稳,响应特性良好,脉冲一致性好,可满足推力装置的使用要求。

Effects of the combined application of organic and chemical nitrogen fertilizer on soil aggregate carbon and nitrogen:A 30-year study

To understand the long-term effects of combined organic and chemical nitrogen fertilization on soil organic C(SOC) and total N(TN), we conducted a 30-year field experiment with a wheat–maize rotation system on the Huang-HuaiHai Plain during 1990–2019. The experimental treatments consisted of five fertilizer regimes: no fertilizer(control), chemical fertilizer only(NPK), chemical fertilizer with straw(NPKS), chemical fertilizer with manure(NPKM), and 1.5 times the rate of NPKM(1.5NPKM). The NPK, NPKS, and NPKM treatments had equal N inputs. The crop yields were measured over the whole experimental duration. Soil samples were collected from the topsoil(0–10 and 10–20 cm) and subsoil(20–40 cm) layers for assessing soil aggregates and taking SOC and TN measurements. Compared with the NPK treatment, the SOC and TN contents increased significantly in both the topsoil(24.1–44.4% for SOC and 22.8–47.7% for TN) and subsoil layers(22.0–47.9% for SOC and 19.8–41.8% for TN) for the organically amended treatments(NPKS, NPKM and 1.5NPKM) after 30 years, while no significant differences were found for the average annual crop yields over the 30 years of the experiment. The 0–10 cm layer of the NPKS treatment and the 20–40 cm layer of the NPKM treatment had significantly higher macroaggregate fraction mass proportions(19.8 and 27.0%) than the NPK treatment. However, the 0–10 and 20–40 cm layers of the 1.5NPKM treatment had significantly lower macroaggregate fraction mass proportions(–19.2 and –29.1%) than the control. The analysis showed that the higher SOC and TN in the soil of organically amended treatments compared to the NPK treatment were related to the increases in SOC and TN protected in the stable fractions(i.e., free microaggregates and microaggregates within macroaggregates), in which the contributions of the stable fractions were 81.1–91.7% of the increase in SOC and 83.3–94.0% of the increase in TN, respectively. The relationships between average C inputs and both stable SOC and TN stocks were significantly positive with R2 values of 0.74 and 0.72(P<0.01) for the whole 40 cm soil profile, which indicates the importance of N for soil C storage. The results of our study provide key evidence that long-term combined organic and chemical nitrogen fertilization, while maintaining reasonable total N inputs, benefited soil C and N storage in both the topsoil and subsoil layers.

N-钙黏蛋白功能研究进展

N-钙黏蛋白(N-cadherin),也称作神经钙黏蛋白(neural-cadherin/Cadherin-2),是一种钙依赖的跨膜糖蛋白,在细胞间粘附、细胞分选、神经发育以及多种生理和病理过程中发挥着关键作用。近年来,随着分子生物学和细胞生物学的快速发展,N-钙黏蛋白的功能研究取得了显著进展,为理解肿瘤转移、神经退行性疾病等提供了重要线索。本文综述了近年来N-钙黏蛋白功能研究的主要进展,包括其分子结构、生物学功能、信号转导机制以及在疾病中的作用,最后总结了N-钙黏蛋白的临床应用前景以及未来研究方向的展望。以期为未来N-cadherin作为新靶点治疗疾病的相关研究和临床应用提供一定的理论参考。