Root morphology and physiology influence aboveground growth and yield formation in rice.However,root morphological and physiological differences among rice varieties with differing nitrogen(N)sensitivities and their r...Root morphology and physiology influence aboveground growth and yield formation in rice.However,root morphological and physiological differences among rice varieties with differing nitrogen(N)sensitivities and their relationship with grain yield are still unclear.In this study,rice varieties differing in N sensitivity over many years of experiments were used.A field experiment with multiple N rates(0,90,180,270,and 360 kg ha^(-1))was conducted to elucidate the effects of N application on root morphology,root physiology,and grain yield.A pot experiment with root excision and exogenous application of 6-benzyladenine(6-BA)at heading stage was used to further verify the above effects.The findings revealed that(1)under the same N application rate,N-insensitive varieties(NIV)had relatively large root biomass(root dry weight,length,and number).Grain yield was associated with root biomass in NIV.The oxidation activity and zeatin(Z)+zeatin riboside(ZR)contents in roots obviously and positively correlated with grain yield in N-sensitive varieties(NSV),and accounted for its higher grain yield than that of NIV at lower N application rates(90 and 180 kg ha^(-1)).(2)The root dry weight required for equal grain yield of NIV was greater than that of NSV.Excision of 1/10 and 1/8 of roots at heading stage had no discernible effect on the yield of Liangyoupeijiu(NIV),and it significantly reduced yield by 11.5%and 21.3%in Tianyouhuazhan(NSV),respectively,compared to the treatment without root excision.The decrease of filled kernels and grain weight after root excision was the primary cause for the yield reduction.Root excision and exogenous 6-BA application after root excision had little influence on the root activity of NIV.The oxidation activity and Z+ZR contents in roots of NSV decreased under root excision,and the increase in the proportion of excised roots aggravated these effects.The application of exogenous 6-BA increased the root activity of NSV and increased filled kernels and grain weight,thereby reducing yield loss after root excision.Thus,the root biomass of NIV was large,and there may be a phenomenon of"root growth redundancy."Vigorous root activity was an essential feature of NSV.Selecting rice varieties with high root activity or increasing root activity by cultivation measures could lead to higher grain yield under lower N application rates.展开更多
Nitrogen fixation is one of the most important sources of new nitrogen in the ocean and thus profoundly affects the nitrogen and carbon biogeochemical processes.The distribution,controlling factors,and flux of N2 fixa...Nitrogen fixation is one of the most important sources of new nitrogen in the ocean and thus profoundly affects the nitrogen and carbon biogeochemical processes.The distribution,controlling factors,and flux of N2 fixation in the global ocean remain uncertain,partly because of the lack of methodological uniformity.The^(15)N_(2)tracer assay(the original bubble method→the^(15)N_(2)-enriched seawater method→the modified bubble method)is the mainstream method for field measurements of N2 fixation rates(NFRs),among which the original bubble method is the most frequently used.However,accumulating evidence has suggested an underestimation of NFRs when using this method.To improve the availability of previous data,we compared NFRs measured by three^(15)N_(2)tracer assays in the South China Sea.Our results indicate that the relationship between NFRs measured by the original bubble method and the^(15)N_(2)-enriched seawater method varies obviously with area and season,which may be influenced by incubation time,diazotrophic composition,and environmental factors.In comparison,the relationship between NFRs measured by the original bubble method and the modified bubble method is more stable,indicating that the N2 fixation rates based on the original bubble methods may be underestimated by approximately 50%.Based on this result,we revised the flux of N2 fixation in the South China Sea to 40 mmol/(m2·a).Our results improve the availability and comparability of literature NFR data in the South China Sea.The comparison of the^(15)N_(2)tracer assay for NFRs measurements on a larger scale is urgently necessary over the global ocean for a more robust understanding of the role of N2 fixation in the marine nitrogen cycle.展开更多
Planting at an optimum density and supplying adequate nitrogen(N) to achieve higher yields is a common practice in crop production, especially for maize(Zea mays L.); however, excessive N fertilizer supply in maiz...Planting at an optimum density and supplying adequate nitrogen(N) to achieve higher yields is a common practice in crop production, especially for maize(Zea mays L.); however, excessive N fertilizer supply in maize production results in reduced N use efficiency(NUE) and severe negative impacts on the environment. This research was conducted to determine the effects of increased plant density and reduced N rate on grain yield, total N uptake, NUE, leaf area index(LAI), intercepted photosynthetically active radiation(IPAR), and resource use efficiency in maize. Field experiments were conducted using a popular maize hybrid Zhengdan 958(ZD958) under different combinations of plant densities and N rates to determine an effective approach for maize production with high yield and high resource use efficiency. Increasing plant density was clearly able to promote N absorption and LAI during the entire growth stage, which allowed high total N uptake and interception of radiation to achieve high dry matter accumulation(DMA), grain yield, NUE, and radiation use efficiency(RUE). However, with an increase in plant density, the demand of N increased along with grain yield. Increasing N rate can significantly increase the DMA, grain yield, LAI, IPAR, and RUE. However, this increase was non-linear and due to the input of too much N fertilizers, the efficiency of N use at NCK(320 kg ha^(–1)) was low. An appropriate reduction in N rate can therefore lead to higher NUE despite a slight loss in grain production. Taking into account both the need for high grain yield and resource use efficiency, a 30% reduction in N supply, and an increase in plant density of 3 plants m^(–2), compared to LD(5.25 plants m^(–2)), would lead to an optimal balance between yield and resource use efficiency.展开更多
Application of fertilizer has been found to significantly affect soil N cycling. However, a comprehensive understanding of the effects of long-term fertilization on soil gross N transformation rates is still lacking. ...Application of fertilizer has been found to significantly affect soil N cycling. However, a comprehensive understanding of the effects of long-term fertilization on soil gross N transformation rates is still lacking. We compiled data of observations from 10 long-term fertilization experiments and conducted a meta-analysis of the effects of long-term fertilization on soil gross N transformation rates. The results showed that if chemical fertilizers of N, P and K were applied in balance, soil p H decreased very slightly. There was a significantly positive effect of long-term fertilization, either chemical or organic fertilizers or their combinations, on gross N mineralization rate compared to the control treatment(the mean effect size ranged from 1.21 to 1.25 at 95% confidence intervals(CI) with a mean of 1.23), mainly due to the increasing soil total N content. The long-term application of organic fertilizer alone and combining organic and chemical fertilizer could increase the mineralization-immobilization turnover, thus enhance available N for plant while reduce N losses potential compared to the control treatment. However, long-term chemical fertilizer application did not significantly affect the gross NH4+ immobilization rate, but accelerated gross nitrification rate(1.19; 95% CI: 1.08 to 1.31). Thus, long-term chemical fertilizer alone would probably induce higher N losses potential through NO3– leaching and runoff than organic fertilizer application compared to the control treatment. Therefore, in the view of the effects of long-term fertilization on gross N transformation rates, it also supports that organic fertilizer alone or combination of organic and chemical fertilizer could not only improve crop yield, but also increase soil fertility and reduce the N losses potential.展开更多
In this paper, field trials in two soils with different Nmin were conducted to study the effects of mineral N content (Nmin) in soil on the maximum yield N rate (MYNR), N recovery of cabbage under spring cabbage-m...In this paper, field trials in two soils with different Nmin were conducted to study the effects of mineral N content (Nmin) in soil on the maximum yield N rate (MYNR), N recovery of cabbage under spring cabbage-maize-winter cabbage rotation system, and the correlation of N fertilization with cabbage yield and quality, and to provide the theoretical basis for N recommendation for high-yield, quality, and safety production of vegetables. The effects of six N rates of 0, 90, 180, 270, 360, and 450 kg ha-1 on the yield, N recovery of spring cabbage, maize, and winter cabbage, water-soluble sugar, Vc, and nitrate content of vegetables were observed. The results showed that soil Nmin had a remarkable influence on the MYNR in the first spring cabbage season. The MYNR for spring cabbage lessened in the soil with high Nmin. Soil Nmin, could be helpful to N recommendation only for the seasonal growing crop because its effects on the following crop yield was less with the active transformation of soil Nmin. The farmer's practice was 1.8-3.2 times higher than the MYNR of cabbage resulted in the nitrate enrichment of groundwater. Both N application rate and Nmin. in the soil profile affected N recovery, whereas, the relay intercropping maize in the cabbage field increased the N recovery at a higher N application rate. Lower N rate (less than 90 kg ha-1) improved the yield and quality of cabbage at the same time; higher N rates increased cabbage yield, but decreased the quality; extremely high N rates of application deceased both yield and quality of cabbage. It was concluded that the soil Nmin had close correlations with MYNR and N recovery of the seasonal growing cabbage. Although the residual effects of the N fertilizer were obvious in Shajiang black meadow soil, cabbage-maize rotation increased the N recovery in treatments with higher N rates. Considering the effects of N rates on cabbage yield and quality, it is necessary to reduce the N rate and lower the yield target for obtaining a better quality of vegetable produce.展开更多
Increasing nitrogen(N)rate could accelerate the decomposition of crop residues,and then improve crop yield by increasing N availability of soil and N uptake of crops.However,it is not clear whether N rate and plant de...Increasing nitrogen(N)rate could accelerate the decomposition of crop residues,and then improve crop yield by increasing N availability of soil and N uptake of crops.However,it is not clear whether N rate and plant density should be modified after a long period of cotton stubble return with high N rate.This study seeks to assess the effects of N rate and plant density on cotton yield,N use efficiency,leaf senescence,soil inorganic N,and apparent N balance in cotton stubble return fields in Liaocheng,China,in 2016 and 2017.Three plant densities 5.25(D_(5.25)),6.75(D_(6.75))and 8.25(D_(8.25))plants m^(-2) and five N rates 0(N_(0)),105(N_(105)),210(N_(210)),315(N315),and 420(N420)kg ha^(-1) were investigated.Compared to the combination used by local farmers(D_(5.25)N_(315)),a 33.3%N reduction and a 28.6%increase in plant density(D_(6.75)N_(210))can maintain high cotton yield,while a 66.7%N reduction at 6.75 plants m^(-2)(D_(6.75)N_(105))can only achieve high yield in the first year.Biological yield increased with the increase of N rate and plant density,and the highest yield was obtained under 420 kg N ha^(-1) at 8.25 plants m^(-2)(D_(8.25)N_(420))across the two years under investigation.Compared to D5.25N315,N agronomic efficiency(NAE)and N recovery efficiency(NRE)in D_(6.75)N_(210) increased by 30.2 and 54.1%,respectively,and NAE and NRE in D6.75N105 increased by 104.8 and 88.1%,respectively.Soil inorganic N decreased sharply under 105 kg N ha^(-1),but no change was found under 210 kg N ha^(-1) at 6.75 plants m^(-2).N deficit occurred under 105 kg N ha^(-1),but it did not occurr under 210 kg N ha^(-1) at 6.75 plants m^(-2).Net photosynthetic rate and N concentration of leaves under N rate ranging from 210 to 420 kg ha^(-1) were higher than those under N rate of 0 or 105 kg N ha^(-1) at all three densities.The findings suggest that D6.75N210 is a superior combination in cotton stubble retaining fields in the Yellow River Valley and other areas with similar ecologies.展开更多
Soil organic carbon (SOC) and soil inorganic carbon (SIC) are important C pools in the Loess Plateau of Northwest China, however, variations of SOC and SIC stocks under different cultivation practices and nitrogen...Soil organic carbon (SOC) and soil inorganic carbon (SIC) are important C pools in the Loess Plateau of Northwest China, however, variations of SOC and SIC stocks under different cultivation practices and nitrogen (N) fertilization rates are not clear in this area. A long-term field experiment started in June 2003 was conducted to investigate the SOC and SIC stocks in a calcareous soil of the Chinese Loess Plateau under four cultivation practices, i.e., fallow (FA), conventional cultivation (CC), straw mulch (SM), and plastic film-mulched ridge and straw-mulched furrow (RF), in combination with three N fertilization rates, i.e., 0 (NO), 120 (N120), and 240 (N240) kg N/hm2. Results indicate that the crop straw addition treatments (SM and RF) increased the contents of soil microbial biomass C (SMBC) and SOC, and the SOC stock increased by 10.1%-13.3% at the upper 20 cm soil depth in comparison to the 8-year fallow (FA) treatment. Meanwhile, SIC stock significantly increased by 19% at the entire tested soil depth range (0-100 cm) under all crop cultivation practices in comparison to that of soil exposed to the long-term fallow treatment, particularly at the upper 60 cm soil depth. Furthermore, moderate N fertilizer application (120 kg N/hm2) increased SOC stock at the upper 40 cm soil depth, whereas SIC stock decreased as the N fertilization rate increased. We conclude that the combined application of crop organic residues and moderate N fertilization rate could facilitate the sequestrations of SOC and SIC in the calcareous soil.展开更多
A field experiment about effects of nitrogen application rates and different NO3-N to NH4-N ratios on agronomic, chemical and biological characteristics as well as yield and quality of flue-cured tobacco grown in a bl...A field experiment about effects of nitrogen application rates and different NO3-N to NH4-N ratios on agronomic, chemical and biological characteristics as well as yield and quality of flue-cured tobacco grown in a black soil was conducted from 2004 to 2005 in Heilongjiang Province. The results showed that the nitrogen application rates at 45 kg·hm^-2 with the ratio of 75% NO3-N to 25% NH4-N resulted in the highest potassium and reducing sugar contents in the flue-cured tobacco leaving with the highest quality grade and value. It is recommended that this ni- trogen application rate and NO3-N to NH4-N ratio should be widely applied on flue-cured tobacco grown in the black soil in Heilongjiang Province.展开更多
[Objectives] This study was conducted to explore the effects of different N application rates and densities on the growth and development of direct-seeding rape as well as on its yield.[Methods] A field experiment was...[Objectives] This study was conducted to explore the effects of different N application rates and densities on the growth and development of direct-seeding rape as well as on its yield.[Methods] A field experiment was carried out by setting five N fertilizer treatments and three density levels.[Results] Increasing seeding density and N application rate could improve the seed yield of rape. The suitable N application rate for the three densities were calculated using the fitted fertilizer efficiency models, respectively, to be 186.77, 221.35 and 236.14 kg/hm^2, at which the yields were the highest. The results showed that in this area, the seeding density of direct-seeding rape could be selected in the range of 6.0×10^5-7.5×10^5 plants/hm^2, and the suitable N application rate could be in the range of 221.35-236.14 kg/hm^2.[Conclusions] This study provides a theoretical basis for the maximization of spatial resource and efficient utilization of fertilizer.展开更多
Background:An optimal N rate is one of the basic determinants for high cotton yield.The purpose of this study was to determine the optimal N rate on a new cotton cropping pattern with late-sowing,high density and onet...Background:An optimal N rate is one of the basic determinants for high cotton yield.The purpose of this study was to determine the optimal N rate on a new cotton cropping pattern with late-sowing,high density and onetime fertilization at the first flower period in Yangtze River Valley,China.A 2-year experiment was conducted in 2015 and 2016 with a randomized complete block design.The cotton growth process,yield,and biomass accumulation were examined.Results:The results showed that N rates had no effect on cotton growing progress or periods.Cotton yield was increased with N rates increasing from 120 to 180 kg·hm^−2,while the yield was not increased when the N rate was beyond 180 kg·hm−2,or even decreased(9∼29%).Cotton had the highest biomass at the N rate of 180 kg·hm^−2 is due to its highest accumulation speed during the fast accumulation period.Conclusions:The result suggests that the N rate for cotton could be reduced further to be 180 kg·hm^−2 under the new cropping pattern in the Yangtze River Valley,China.展开更多
Basing on the DFT calculations we propose the new theoretical model which describes both the surface tension σ of the short chain n-alkanes at their normal boiling points and their reaction rate constants with hydrox...Basing on the DFT calculations we propose the new theoretical model which describes both the surface tension σ of the short chain n-alkanes at their normal boiling points and their reaction rate constants with hydroxyl radicals OH<span style="white-space:nowrap;">•</span> (at 297 ± 2 K) on the basis of their molecular orbital electronic characteristics. It has been shown that intermolecular dispersion attraction within the surface liquid monolayer of these compounds, as well as their reaction rate constants k with OH<span style="white-space:nowrap;">•</span> radicals are determined by the energies <em>E<sub>orb</sub></em> of the specific occupied molecular orbitals which are the same in the determination of both the above physico-chemical characteristics of the studied n-alkanes. The received regression equations confirm the theoretically found dependences between the quantities of σ and k and the module |<em>E<sub>orb</sub></em>|. For the compounds under study this fact indicates the key role of their electronic structure particularities in determination of both the physical (surface tension) and the chemical (reaction rate constants) properties.展开更多
The new theoretical models describe both the solubility S of the shot chain n-alkanes in water at 298.15 K, and their reaction rate constants k with nitronium cation NO<sub>2</sub><sup>+ </sup>...The new theoretical models describe both the solubility S of the shot chain n-alkanes in water at 298.15 K, and their reaction rate constants k with nitronium cation NO<sub>2</sub><sup>+ </sup>at 293.15 K on the basis of their molecular orbital characteristics. It is shown that both the quantities S and k are determined by the energies E<sub>orb</sub> of the specific virtual (for S) and occupied (for k) molecular orbitals of these n-alkanes. The obtained regression equations confirm the theoretically found dependences of S and k on the absolute value of E<sub>orb</sub>. This fact demonstrates that the electronic structure particularities of the studied n-alkanes play a crucial role in both their above-mentioned physicochemical properties.展开更多
Developing high-yielding rice(Oryza sativa L.)cultivars depends on having a better understanding of nitrogen(N) accumulation and translocation to the ear during the reproductive stage.Field experiments were carried ou...Developing high-yielding rice(Oryza sativa L.)cultivars depends on having a better understanding of nitrogen(N) accumulation and translocation to the ear during the reproductive stage.Field experiments were carried out to evaluate the genetic variation for N accumulation and translocation in different Japonica rice cultivars at different N rates and to identify any relationship to grain yield in southeast China.Four Japonica cultivars with similar agronomic characteristics were grown at two experimental sites in 2004 with three N rates of 0,60,and 180 kg N ha^(-1).Dry weights and N contents of rice plants were measured at tillering,initiation,anthesis,and maturity.Grain yields exhibited significant differences (P<0.05)among the cultivars and N application rates.Increasing N rates improved N uptake at anthesis and maturity in all four cultivars(P<0.05).N translocation from vegetative organs to the grains increased with enhanced N rates (P<0.05).N translocation to the grains ranged from 9 to 64 kg N ha^(-1)and N-translocation efficiency from 33% to 68%. Grain yield was linear to N uptake at anthesis(r^2=0.78^(**))and N translocation(r^2=0.67^(**)).Thus,cultivars with a high N uptake at anthesis,low residual N in the straw at maturity,and appropriate low N fertilizer supply in southeast China should efficiently increase N-recovery rate while maintaining grain yield and soil fertility.展开更多
The variability in weather patterns consequent upon climate change constitutes a critical factor influencing soil N availability and the performance of crops. This paper aimed at evaluating the effects of climatic fac...The variability in weather patterns consequent upon climate change constitutes a critical factor influencing soil N availability and the performance of crops. This paper aimed at evaluating the effects of climatic factors on soybean subjected to low N rates under rain-fed situation in the southern Guinea agro-ecology of Oyo State, Nigeria. A 2-year field experiment involving two soybean varieties (TGx1485-1D and TGx1448-2E) and five low rates of N fertilizer application (0, 5, 15, 25, 35 kg/ha) using Urea applied by banding 7 days after planting was arranged in a 5 × 2 split-plot with three replications. N rates constitute the main plot while variety constitutes the sub-plot. Parameters measured include dry shoot weight, shoot N accumulation, and grain yield. Data were subjected to GENSTAT statistical package for analysis, and means separated with Duncan Multiple Range Test (DMRT) at 5% level of probability. Climatic parameters of rainfall, temperature, and potential evapotranspiration data were collected from the surface observatory of the National meteorological agency (NIMET) and subjected to Excel package for computation and graphics. The dry shoot weight increases as N rate increases up to 25 kg/ha, but declines at 35 kg/ha application rate, however, TGx1448-2E produced a higher dry shoot weight (2.9 t/ha) than TGx1485-1D (2.8 t/ha). Low N rates had no significant effects on shoot N accumulation and there was no significant difference in varietal response. Low N rate did not affect grain yield, but the TGx1448-2E had a greater yield of 1.5 t/ha than TGx1485-1D (1.30 t/ha). Annual rainfall amounts were 935.5 mm and1475.8 mm in 2009 and 2010 respectively. Potential evapotranspiration (PET) values were 1676.5 mm and 1676.8 mm in 2009 and 2010 respectively. Temperature values range from 24<span style="white-space:nowrap;"><span style="white-space:nowrap;"><span style="white-space:nowrap;">˚</span></span></span>C to 29.8<span style="white-space:nowrap;"><span style="white-space:nowrap;"><span style="white-space:nowrap;">˚</span></span></span>C in both years and the mean monthly temperature for 2009 was 26.5<span style="white-space:nowrap;"><span style="white-space:nowrap;"><span style="white-space:nowrap;">˚</span></span></span>C and 27.1<span style="white-space:nowrap;"><span style="white-space:nowrap;"><span style="white-space:nowrap;">˚</span></span></span>C for 2010. The application of N fertilizer to soybean requires appropriate timing for effective use. Climatic parameters such as rainfall, temperature, and evapotranspiration have dire consideration for fertilizer use and efficiency.展开更多
Afforestation effectively improved soil microbial communities and significantly increased soil nitro-gen mineralization rate(Rm).Soil microorganisms drive Rm by regulating soil N-cycling genes.Soil nitrification genes...Afforestation effectively improved soil microbial communities and significantly increased soil nitro-gen mineralization rate(Rm).Soil microorganisms drive Rm by regulating soil N-cycling genes.Soil nitrification genes had a major effect on soil Rm than denitrification genes after afforestation.Assessing the function of forest ecosystems requires an understanding of the mechanism of soil nitrogen mineralization.However,it remains unclear how soil N-cycling genes drive soil nitrogen mineralization during afforestation.In this study,we collected soil samples from a chrono-sequence of 14,20,30,and 45 years of Robinia pseudoacacia L.(RP14,RP20,RP30,and RP45)with a sloped farmland(FL)as a control.Through metagenomic sequencing analysis,we found significant changes in the diversity and composition of soil microbial communities involved in N-cycling along the afforestation time series,with afforestation effectively increasing the diversity(both alpha and beta diversity)of soil microbial communities.We conducted indoor culture experiments and analyzed correlations,which revealed a significant increase in both soil nitrification rate(Rn)and soil nitrogen mineralization rate(Rm)with increasing stand age.Furthermore,we found a strong correlation between soil Rm and soil microbial diversity(both alpha and beta diversity)and with the abundance of soil N-cycling genes.Partial least squares path modeling(PLS-PM)analysis showed that nitrification genes(narH,narY,nxrB,narG,narZ,nxrA,hao,pmoC-amoC)and denitrification genes(norB,nosZ,nirK)had a greater direct effect on soil Rm compared to their effect on soil microbial communities.Our results reveal the relationships between soil nitrogen mineralization rate and soil microbial communities and between the mineralization rate and functional genes involved in N-cycling,in the context of Robinia pseudoacacia L.restoration on the Loess Plateau.This study enriches the understanding of the effects of microorganisms on soil nitrogen mineralization rate during afforestation and provides a new theoretical basis for evaluating soil nitrogen mineralization mechanisms during forest succession.展开更多
Seven-day-old maize (Zea mays) plants were grown hydroponically for ten days in N-deprived nutrient solution. The distribution profiles according to the position on the stem of the –N laminas stomatal conductance, tr...Seven-day-old maize (Zea mays) plants were grown hydroponically for ten days in N-deprived nutrient solution. The distribution profiles according to the position on the stem of the –N laminas stomatal conductance, transpiration rate, photosynthetic rate (1st-group) were monitored, along with the corresponding profiles of dry mass, water amount, water content, length, surface area, and specific surface area (2nd-group), relative to control. In the uppermost –N laminas, the changes within a parameter of the 1st-group were significantly higher and of the 2nd-group significantly lower than the control, respectively. Correlations of the corresponding values among the parameters of the 1st-or 2nd-group were linear. The parameters between groups correlated non-linearly. Transpiration rate was divided by the lamina’s dry mass correlated with surface area in a power-type function. The slopes of the response ratios linear relations between the various pairs of parameters could be used for simulation of a lamina’s response to the deprivation.展开更多
Technologies for reducing corn leaf burn caused by foliar spray of urea-ammonium nitrate (UAN) during the early growing season are limited. A field experiment was carried out to evaluate the effects of humic acid on c...Technologies for reducing corn leaf burn caused by foliar spray of urea-ammonium nitrate (UAN) during the early growing season are limited. A field experiment was carried out to evaluate the effects of humic acid on corn leaf burn caused by foliar spray of undiluted UAN solution on corn canopy at Jackson, TN in 2018. Thirteen treatments of the mixtures of UAN and humic acid were evaluated at V6 of corn with different UAN application rates and different UAN/humic acid ratios. Leaf burn during 1 2, 3, 4, 5, 6, 7, and 14 days after UAN foliar spray significantly differed between with or without humic acid addition. The addition of humic acid to UAN significantly reduced leaf burn at each UAN application rate (15, 25, and 35 gal/acre). The reduction of leaf burn was enhanced as the humic acid/UAN ratio went up from 10% to 30%. Leaf burn due to foliar application of UAN became severer with higher UAN rates. The linear regression of leaf burn 14 days after application with humic acid/UAN ratio was highly significant and negative. However, the linear regression of leaf burn 14 days after application with the UAN application rate was highly significant and positive. In conclusion, adding humic acid to foliar-applied UAN is beneficial for reducing corn leaf burn during the early growing season.展开更多
Excessive use of N fertilizers(driven by high-yield goals)and its consequent environmental problems are becoming increasingly acute in agricultural systems.A 2-year field experiment was conducted to investigate the ef...Excessive use of N fertilizers(driven by high-yield goals)and its consequent environmental problems are becoming increasingly acute in agricultural systems.A 2-year field experiment was conducted to investigate the effects of three N application methods(application of solid granular urea once(OF)or twice(TF),application of solid granular urea mixed with controlled-release urea once(MF),and six N rates(0,60,120,180,240,and 300 kg N ha^(-1))on maize yield,economic benefits,N use efficiency,and soil N balance in the maize(Zea mays L.)film mulching system on the Loess Plateau,China.The grain yield and economic return of maize were significantly affected by the N rate and application method.Compared with the OF treatment,the MF treatment not only increased the maize yield(increased by 9.0-16.7%)but also improved the economic return(increased by 10.9-25.8%).The agronomic N use efficiency(NAE),N partial factor productivity(NPFP)and recovery N efficiency(NRE)were significantly improved by 19.3-66.7,9.0-16.7 and 40.2-71.5%,respectively,compared with the OF treatment.The economic optimal N rate(EONR)of the OF,TF,and MF was 145.6,147.2,and 144.9 kg ha^(-1) in 2019,and 206.4,186.4,and 146.0 kg ha^(-1) in 2020,respectively.The apparent soil N loss at EONR of the OF,TF,and MF were 97.1-100.5,78.5-79.3,and 50.5-68.1 kg ha^(-1),respectively.These results support MF as a one-time N application method for delivering high yields and economic benefits,with low N input requirements within film mulching spring maize system on the Loess Plateau.展开更多
基金the National Natural Science Foundation of China(32071947,32071944,31871557)the Open Project of Jiangsu Key Laboratory of Crop Genetics and Physiology(YCSL202102)+1 种基金the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)the Postgraduate Research&Practice Innovation Program of Jiangsu province(KYCX21_3235).
文摘Root morphology and physiology influence aboveground growth and yield formation in rice.However,root morphological and physiological differences among rice varieties with differing nitrogen(N)sensitivities and their relationship with grain yield are still unclear.In this study,rice varieties differing in N sensitivity over many years of experiments were used.A field experiment with multiple N rates(0,90,180,270,and 360 kg ha^(-1))was conducted to elucidate the effects of N application on root morphology,root physiology,and grain yield.A pot experiment with root excision and exogenous application of 6-benzyladenine(6-BA)at heading stage was used to further verify the above effects.The findings revealed that(1)under the same N application rate,N-insensitive varieties(NIV)had relatively large root biomass(root dry weight,length,and number).Grain yield was associated with root biomass in NIV.The oxidation activity and zeatin(Z)+zeatin riboside(ZR)contents in roots obviously and positively correlated with grain yield in N-sensitive varieties(NSV),and accounted for its higher grain yield than that of NIV at lower N application rates(90 and 180 kg ha^(-1)).(2)The root dry weight required for equal grain yield of NIV was greater than that of NSV.Excision of 1/10 and 1/8 of roots at heading stage had no discernible effect on the yield of Liangyoupeijiu(NIV),and it significantly reduced yield by 11.5%and 21.3%in Tianyouhuazhan(NSV),respectively,compared to the treatment without root excision.The decrease of filled kernels and grain weight after root excision was the primary cause for the yield reduction.Root excision and exogenous 6-BA application after root excision had little influence on the root activity of NIV.The oxidation activity and Z+ZR contents in roots of NSV decreased under root excision,and the increase in the proportion of excised roots aggravated these effects.The application of exogenous 6-BA increased the root activity of NSV and increased filled kernels and grain weight,thereby reducing yield loss after root excision.Thus,the root biomass of NIV was large,and there may be a phenomenon of"root growth redundancy."Vigorous root activity was an essential feature of NSV.Selecting rice varieties with high root activity or increasing root activity by cultivation measures could lead to higher grain yield under lower N application rates.
基金The National Natural Science Foundation of China under contract Nos 42076042 and 41721005the Fund of Ministry of Science and Technology of China under contract No.2017FY201403the Fund of China Ocean Mineral Resources R&D Association under contract No.DY135-13-E2-03.
文摘Nitrogen fixation is one of the most important sources of new nitrogen in the ocean and thus profoundly affects the nitrogen and carbon biogeochemical processes.The distribution,controlling factors,and flux of N2 fixation in the global ocean remain uncertain,partly because of the lack of methodological uniformity.The^(15)N_(2)tracer assay(the original bubble method→the^(15)N_(2)-enriched seawater method→the modified bubble method)is the mainstream method for field measurements of N2 fixation rates(NFRs),among which the original bubble method is the most frequently used.However,accumulating evidence has suggested an underestimation of NFRs when using this method.To improve the availability of previous data,we compared NFRs measured by three^(15)N_(2)tracer assays in the South China Sea.Our results indicate that the relationship between NFRs measured by the original bubble method and the^(15)N_(2)-enriched seawater method varies obviously with area and season,which may be influenced by incubation time,diazotrophic composition,and environmental factors.In comparison,the relationship between NFRs measured by the original bubble method and the modified bubble method is more stable,indicating that the N2 fixation rates based on the original bubble methods may be underestimated by approximately 50%.Based on this result,we revised the flux of N2 fixation in the South China Sea to 40 mmol/(m2·a).Our results improve the availability and comparability of literature NFR data in the South China Sea.The comparison of the^(15)N_(2)tracer assay for NFRs measurements on a larger scale is urgently necessary over the global ocean for a more robust understanding of the role of N2 fixation in the marine nitrogen cycle.
基金the National Natural Science Foundation of China(3117 1497)the National Basic Research Program of China(973 Program,2011CB100105)+1 种基金the National Food Science and Technology of High Yield Program of China(2011BAD16B09)the Special Fund for Agro-scientific Research in the Public Interest of China(201203096)
文摘Planting at an optimum density and supplying adequate nitrogen(N) to achieve higher yields is a common practice in crop production, especially for maize(Zea mays L.); however, excessive N fertilizer supply in maize production results in reduced N use efficiency(NUE) and severe negative impacts on the environment. This research was conducted to determine the effects of increased plant density and reduced N rate on grain yield, total N uptake, NUE, leaf area index(LAI), intercepted photosynthetically active radiation(IPAR), and resource use efficiency in maize. Field experiments were conducted using a popular maize hybrid Zhengdan 958(ZD958) under different combinations of plant densities and N rates to determine an effective approach for maize production with high yield and high resource use efficiency. Increasing plant density was clearly able to promote N absorption and LAI during the entire growth stage, which allowed high total N uptake and interception of radiation to achieve high dry matter accumulation(DMA), grain yield, NUE, and radiation use efficiency(RUE). However, with an increase in plant density, the demand of N increased along with grain yield. Increasing N rate can significantly increase the DMA, grain yield, LAI, IPAR, and RUE. However, this increase was non-linear and due to the input of too much N fertilizers, the efficiency of N use at NCK(320 kg ha^(–1)) was low. An appropriate reduction in N rate can therefore lead to higher NUE despite a slight loss in grain production. Taking into account both the need for high grain yield and resource use efficiency, a 30% reduction in N supply, and an increase in plant density of 3 plants m^(–2), compared to LD(5.25 plants m^(–2)), would lead to an optimal balance between yield and resource use efficiency.
基金supported by the National Natural Science Foundation of China (41330744)the “973” Program of China (2014CB953803)the Priority Academic Program Development of Jiangsu Higher Education Institutions, China (164320H116)
文摘Application of fertilizer has been found to significantly affect soil N cycling. However, a comprehensive understanding of the effects of long-term fertilization on soil gross N transformation rates is still lacking. We compiled data of observations from 10 long-term fertilization experiments and conducted a meta-analysis of the effects of long-term fertilization on soil gross N transformation rates. The results showed that if chemical fertilizers of N, P and K were applied in balance, soil p H decreased very slightly. There was a significantly positive effect of long-term fertilization, either chemical or organic fertilizers or their combinations, on gross N mineralization rate compared to the control treatment(the mean effect size ranged from 1.21 to 1.25 at 95% confidence intervals(CI) with a mean of 1.23), mainly due to the increasing soil total N content. The long-term application of organic fertilizer alone and combining organic and chemical fertilizer could increase the mineralization-immobilization turnover, thus enhance available N for plant while reduce N losses potential compared to the control treatment. However, long-term chemical fertilizer application did not significantly affect the gross NH4+ immobilization rate, but accelerated gross nitrification rate(1.19; 95% CI: 1.08 to 1.31). Thus, long-term chemical fertilizer alone would probably induce higher N losses potential through NO3– leaching and runoff than organic fertilizer application compared to the control treatment. Therefore, in the view of the effects of long-term fertilization on gross N transformation rates, it also supports that organic fertilizer alone or combination of organic and chemical fertilizer could not only improve crop yield, but also increase soil fertility and reduce the N losses potential.
文摘In this paper, field trials in two soils with different Nmin were conducted to study the effects of mineral N content (Nmin) in soil on the maximum yield N rate (MYNR), N recovery of cabbage under spring cabbage-maize-winter cabbage rotation system, and the correlation of N fertilization with cabbage yield and quality, and to provide the theoretical basis for N recommendation for high-yield, quality, and safety production of vegetables. The effects of six N rates of 0, 90, 180, 270, 360, and 450 kg ha-1 on the yield, N recovery of spring cabbage, maize, and winter cabbage, water-soluble sugar, Vc, and nitrate content of vegetables were observed. The results showed that soil Nmin had a remarkable influence on the MYNR in the first spring cabbage season. The MYNR for spring cabbage lessened in the soil with high Nmin. Soil Nmin, could be helpful to N recommendation only for the seasonal growing crop because its effects on the following crop yield was less with the active transformation of soil Nmin. The farmer's practice was 1.8-3.2 times higher than the MYNR of cabbage resulted in the nitrate enrichment of groundwater. Both N application rate and Nmin. in the soil profile affected N recovery, whereas, the relay intercropping maize in the cabbage field increased the N recovery at a higher N application rate. Lower N rate (less than 90 kg ha-1) improved the yield and quality of cabbage at the same time; higher N rates increased cabbage yield, but decreased the quality; extremely high N rates of application deceased both yield and quality of cabbage. It was concluded that the soil Nmin had close correlations with MYNR and N recovery of the seasonal growing cabbage. Although the residual effects of the N fertilizer were obvious in Shajiang black meadow soil, cabbage-maize rotation increased the N recovery in treatments with higher N rates. Considering the effects of N rates on cabbage yield and quality, it is necessary to reduce the N rate and lower the yield target for obtaining a better quality of vegetable produce.
基金The study was supported by the National Natural Science Foundation of China(31601253)the Natural Science Foundation of Shandong Province,China(ZR2016CQ20)+1 种基金the China Postdoctoral Science Foundation(2017M610438)the Modern Agro-industry Technology Research System of Shandong Province,China(SDAIT-03-03/05).
文摘Increasing nitrogen(N)rate could accelerate the decomposition of crop residues,and then improve crop yield by increasing N availability of soil and N uptake of crops.However,it is not clear whether N rate and plant density should be modified after a long period of cotton stubble return with high N rate.This study seeks to assess the effects of N rate and plant density on cotton yield,N use efficiency,leaf senescence,soil inorganic N,and apparent N balance in cotton stubble return fields in Liaocheng,China,in 2016 and 2017.Three plant densities 5.25(D_(5.25)),6.75(D_(6.75))and 8.25(D_(8.25))plants m^(-2) and five N rates 0(N_(0)),105(N_(105)),210(N_(210)),315(N315),and 420(N420)kg ha^(-1) were investigated.Compared to the combination used by local farmers(D_(5.25)N_(315)),a 33.3%N reduction and a 28.6%increase in plant density(D_(6.75)N_(210))can maintain high cotton yield,while a 66.7%N reduction at 6.75 plants m^(-2)(D_(6.75)N_(105))can only achieve high yield in the first year.Biological yield increased with the increase of N rate and plant density,and the highest yield was obtained under 420 kg N ha^(-1) at 8.25 plants m^(-2)(D_(8.25)N_(420))across the two years under investigation.Compared to D5.25N315,N agronomic efficiency(NAE)and N recovery efficiency(NRE)in D_(6.75)N_(210) increased by 30.2 and 54.1%,respectively,and NAE and NRE in D6.75N105 increased by 104.8 and 88.1%,respectively.Soil inorganic N decreased sharply under 105 kg N ha^(-1),but no change was found under 210 kg N ha^(-1) at 6.75 plants m^(-2).N deficit occurred under 105 kg N ha^(-1),but it did not occurr under 210 kg N ha^(-1) at 6.75 plants m^(-2).Net photosynthetic rate and N concentration of leaves under N rate ranging from 210 to 420 kg ha^(-1) were higher than those under N rate of 0 or 105 kg N ha^(-1) at all three densities.The findings suggest that D6.75N210 is a superior combination in cotton stubble retaining fields in the Yellow River Valley and other areas with similar ecologies.
基金supported by the National Natural Science Foundation of China (41671295)the National Technology R&D Pillar Program in the 12th Five Year Plan of China (2012BAD15B04)
文摘Soil organic carbon (SOC) and soil inorganic carbon (SIC) are important C pools in the Loess Plateau of Northwest China, however, variations of SOC and SIC stocks under different cultivation practices and nitrogen (N) fertilization rates are not clear in this area. A long-term field experiment started in June 2003 was conducted to investigate the SOC and SIC stocks in a calcareous soil of the Chinese Loess Plateau under four cultivation practices, i.e., fallow (FA), conventional cultivation (CC), straw mulch (SM), and plastic film-mulched ridge and straw-mulched furrow (RF), in combination with three N fertilization rates, i.e., 0 (NO), 120 (N120), and 240 (N240) kg N/hm2. Results indicate that the crop straw addition treatments (SM and RF) increased the contents of soil microbial biomass C (SMBC) and SOC, and the SOC stock increased by 10.1%-13.3% at the upper 20 cm soil depth in comparison to the 8-year fallow (FA) treatment. Meanwhile, SIC stock significantly increased by 19% at the entire tested soil depth range (0-100 cm) under all crop cultivation practices in comparison to that of soil exposed to the long-term fallow treatment, particularly at the upper 60 cm soil depth. Furthermore, moderate N fertilizer application (120 kg N/hm2) increased SOC stock at the upper 40 cm soil depth, whereas SIC stock decreased as the N fertilization rate increased. We conclude that the combined application of crop organic residues and moderate N fertilization rate could facilitate the sequestrations of SOC and SIC in the calcareous soil.
文摘A field experiment about effects of nitrogen application rates and different NO3-N to NH4-N ratios on agronomic, chemical and biological characteristics as well as yield and quality of flue-cured tobacco grown in a black soil was conducted from 2004 to 2005 in Heilongjiang Province. The results showed that the nitrogen application rates at 45 kg·hm^-2 with the ratio of 75% NO3-N to 25% NH4-N resulted in the highest potassium and reducing sugar contents in the flue-cured tobacco leaving with the highest quality grade and value. It is recommended that this ni- trogen application rate and NO3-N to NH4-N ratio should be widely applied on flue-cured tobacco grown in the black soil in Heilongjiang Province.
文摘[Objectives] This study was conducted to explore the effects of different N application rates and densities on the growth and development of direct-seeding rape as well as on its yield.[Methods] A field experiment was carried out by setting five N fertilizer treatments and three density levels.[Results] Increasing seeding density and N application rate could improve the seed yield of rape. The suitable N application rate for the three densities were calculated using the fitted fertilizer efficiency models, respectively, to be 186.77, 221.35 and 236.14 kg/hm^2, at which the yields were the highest. The results showed that in this area, the seeding density of direct-seeding rape could be selected in the range of 6.0×10^5-7.5×10^5 plants/hm^2, and the suitable N application rate could be in the range of 221.35-236.14 kg/hm^2.[Conclusions] This study provides a theoretical basis for the maximization of spatial resource and efficient utilization of fertilizer.
基金This project was supported by the National Natural Science Foundation of China(31271665).
文摘Background:An optimal N rate is one of the basic determinants for high cotton yield.The purpose of this study was to determine the optimal N rate on a new cotton cropping pattern with late-sowing,high density and onetime fertilization at the first flower period in Yangtze River Valley,China.A 2-year experiment was conducted in 2015 and 2016 with a randomized complete block design.The cotton growth process,yield,and biomass accumulation were examined.Results:The results showed that N rates had no effect on cotton growing progress or periods.Cotton yield was increased with N rates increasing from 120 to 180 kg·hm^−2,while the yield was not increased when the N rate was beyond 180 kg·hm−2,or even decreased(9∼29%).Cotton had the highest biomass at the N rate of 180 kg·hm^−2 is due to its highest accumulation speed during the fast accumulation period.Conclusions:The result suggests that the N rate for cotton could be reduced further to be 180 kg·hm^−2 under the new cropping pattern in the Yangtze River Valley,China.
文摘Basing on the DFT calculations we propose the new theoretical model which describes both the surface tension σ of the short chain n-alkanes at their normal boiling points and their reaction rate constants with hydroxyl radicals OH<span style="white-space:nowrap;">•</span> (at 297 ± 2 K) on the basis of their molecular orbital electronic characteristics. It has been shown that intermolecular dispersion attraction within the surface liquid monolayer of these compounds, as well as their reaction rate constants k with OH<span style="white-space:nowrap;">•</span> radicals are determined by the energies <em>E<sub>orb</sub></em> of the specific occupied molecular orbitals which are the same in the determination of both the above physico-chemical characteristics of the studied n-alkanes. The received regression equations confirm the theoretically found dependences between the quantities of σ and k and the module |<em>E<sub>orb</sub></em>|. For the compounds under study this fact indicates the key role of their electronic structure particularities in determination of both the physical (surface tension) and the chemical (reaction rate constants) properties.
文摘The new theoretical models describe both the solubility S of the shot chain n-alkanes in water at 298.15 K, and their reaction rate constants k with nitronium cation NO<sub>2</sub><sup>+ </sup>at 293.15 K on the basis of their molecular orbital characteristics. It is shown that both the quantities S and k are determined by the energies E<sub>orb</sub> of the specific virtual (for S) and occupied (for k) molecular orbitals of these n-alkanes. The obtained regression equations confirm the theoretically found dependences of S and k on the absolute value of E<sub>orb</sub>. This fact demonstrates that the electronic structure particularities of the studied n-alkanes play a crucial role in both their above-mentioned physicochemical properties.
基金Project supported by the National Natural Science Foundation of China(Nos.30390082 and 3027079)the Science and Technology Committee of Jiangsu Province,China(No.BE2005369).
文摘Developing high-yielding rice(Oryza sativa L.)cultivars depends on having a better understanding of nitrogen(N) accumulation and translocation to the ear during the reproductive stage.Field experiments were carried out to evaluate the genetic variation for N accumulation and translocation in different Japonica rice cultivars at different N rates and to identify any relationship to grain yield in southeast China.Four Japonica cultivars with similar agronomic characteristics were grown at two experimental sites in 2004 with three N rates of 0,60,and 180 kg N ha^(-1).Dry weights and N contents of rice plants were measured at tillering,initiation,anthesis,and maturity.Grain yields exhibited significant differences (P<0.05)among the cultivars and N application rates.Increasing N rates improved N uptake at anthesis and maturity in all four cultivars(P<0.05).N translocation from vegetative organs to the grains increased with enhanced N rates (P<0.05).N translocation to the grains ranged from 9 to 64 kg N ha^(-1)and N-translocation efficiency from 33% to 68%. Grain yield was linear to N uptake at anthesis(r^2=0.78^(**))and N translocation(r^2=0.67^(**)).Thus,cultivars with a high N uptake at anthesis,low residual N in the straw at maturity,and appropriate low N fertilizer supply in southeast China should efficiently increase N-recovery rate while maintaining grain yield and soil fertility.
文摘The variability in weather patterns consequent upon climate change constitutes a critical factor influencing soil N availability and the performance of crops. This paper aimed at evaluating the effects of climatic factors on soybean subjected to low N rates under rain-fed situation in the southern Guinea agro-ecology of Oyo State, Nigeria. A 2-year field experiment involving two soybean varieties (TGx1485-1D and TGx1448-2E) and five low rates of N fertilizer application (0, 5, 15, 25, 35 kg/ha) using Urea applied by banding 7 days after planting was arranged in a 5 × 2 split-plot with three replications. N rates constitute the main plot while variety constitutes the sub-plot. Parameters measured include dry shoot weight, shoot N accumulation, and grain yield. Data were subjected to GENSTAT statistical package for analysis, and means separated with Duncan Multiple Range Test (DMRT) at 5% level of probability. Climatic parameters of rainfall, temperature, and potential evapotranspiration data were collected from the surface observatory of the National meteorological agency (NIMET) and subjected to Excel package for computation and graphics. The dry shoot weight increases as N rate increases up to 25 kg/ha, but declines at 35 kg/ha application rate, however, TGx1448-2E produced a higher dry shoot weight (2.9 t/ha) than TGx1485-1D (2.8 t/ha). Low N rates had no significant effects on shoot N accumulation and there was no significant difference in varietal response. Low N rate did not affect grain yield, but the TGx1448-2E had a greater yield of 1.5 t/ha than TGx1485-1D (1.30 t/ha). Annual rainfall amounts were 935.5 mm and1475.8 mm in 2009 and 2010 respectively. Potential evapotranspiration (PET) values were 1676.5 mm and 1676.8 mm in 2009 and 2010 respectively. Temperature values range from 24<span style="white-space:nowrap;"><span style="white-space:nowrap;"><span style="white-space:nowrap;">˚</span></span></span>C to 29.8<span style="white-space:nowrap;"><span style="white-space:nowrap;"><span style="white-space:nowrap;">˚</span></span></span>C in both years and the mean monthly temperature for 2009 was 26.5<span style="white-space:nowrap;"><span style="white-space:nowrap;"><span style="white-space:nowrap;">˚</span></span></span>C and 27.1<span style="white-space:nowrap;"><span style="white-space:nowrap;"><span style="white-space:nowrap;">˚</span></span></span>C for 2010. The application of N fertilizer to soybean requires appropriate timing for effective use. Climatic parameters such as rainfall, temperature, and evapotranspiration have dire consideration for fertilizer use and efficiency.
基金supported by the National Natural Science Foundation of China(No.41907031)the China Postdoctoral Science Foundation(No.2021T140565)the China Postdoctoral Science Foundation(No.2019M650276).
文摘Afforestation effectively improved soil microbial communities and significantly increased soil nitro-gen mineralization rate(Rm).Soil microorganisms drive Rm by regulating soil N-cycling genes.Soil nitrification genes had a major effect on soil Rm than denitrification genes after afforestation.Assessing the function of forest ecosystems requires an understanding of the mechanism of soil nitrogen mineralization.However,it remains unclear how soil N-cycling genes drive soil nitrogen mineralization during afforestation.In this study,we collected soil samples from a chrono-sequence of 14,20,30,and 45 years of Robinia pseudoacacia L.(RP14,RP20,RP30,and RP45)with a sloped farmland(FL)as a control.Through metagenomic sequencing analysis,we found significant changes in the diversity and composition of soil microbial communities involved in N-cycling along the afforestation time series,with afforestation effectively increasing the diversity(both alpha and beta diversity)of soil microbial communities.We conducted indoor culture experiments and analyzed correlations,which revealed a significant increase in both soil nitrification rate(Rn)and soil nitrogen mineralization rate(Rm)with increasing stand age.Furthermore,we found a strong correlation between soil Rm and soil microbial diversity(both alpha and beta diversity)and with the abundance of soil N-cycling genes.Partial least squares path modeling(PLS-PM)analysis showed that nitrification genes(narH,narY,nxrB,narG,narZ,nxrA,hao,pmoC-amoC)and denitrification genes(norB,nosZ,nirK)had a greater direct effect on soil Rm compared to their effect on soil microbial communities.Our results reveal the relationships between soil nitrogen mineralization rate and soil microbial communities and between the mineralization rate and functional genes involved in N-cycling,in the context of Robinia pseudoacacia L.restoration on the Loess Plateau.This study enriches the understanding of the effects of microorganisms on soil nitrogen mineralization rate during afforestation and provides a new theoretical basis for evaluating soil nitrogen mineralization mechanisms during forest succession.
文摘Seven-day-old maize (Zea mays) plants were grown hydroponically for ten days in N-deprived nutrient solution. The distribution profiles according to the position on the stem of the –N laminas stomatal conductance, transpiration rate, photosynthetic rate (1st-group) were monitored, along with the corresponding profiles of dry mass, water amount, water content, length, surface area, and specific surface area (2nd-group), relative to control. In the uppermost –N laminas, the changes within a parameter of the 1st-group were significantly higher and of the 2nd-group significantly lower than the control, respectively. Correlations of the corresponding values among the parameters of the 1st-or 2nd-group were linear. The parameters between groups correlated non-linearly. Transpiration rate was divided by the lamina’s dry mass correlated with surface area in a power-type function. The slopes of the response ratios linear relations between the various pairs of parameters could be used for simulation of a lamina’s response to the deprivation.
文摘Technologies for reducing corn leaf burn caused by foliar spray of urea-ammonium nitrate (UAN) during the early growing season are limited. A field experiment was carried out to evaluate the effects of humic acid on corn leaf burn caused by foliar spray of undiluted UAN solution on corn canopy at Jackson, TN in 2018. Thirteen treatments of the mixtures of UAN and humic acid were evaluated at V6 of corn with different UAN application rates and different UAN/humic acid ratios. Leaf burn during 1 2, 3, 4, 5, 6, 7, and 14 days after UAN foliar spray significantly differed between with or without humic acid addition. The addition of humic acid to UAN significantly reduced leaf burn at each UAN application rate (15, 25, and 35 gal/acre). The reduction of leaf burn was enhanced as the humic acid/UAN ratio went up from 10% to 30%. Leaf burn due to foliar application of UAN became severer with higher UAN rates. The linear regression of leaf burn 14 days after application with humic acid/UAN ratio was highly significant and negative. However, the linear regression of leaf burn 14 days after application with the UAN application rate was highly significant and positive. In conclusion, adding humic acid to foliar-applied UAN is beneficial for reducing corn leaf burn during the early growing season.
基金financially supported by the National Key R&D Program of China (2022YFD1900702 and 2021YFD1900700)the Key Research and Development Program of Shaanxi, China (2023-ZDLNY-52)the National Natural Science Foundation of China (42077102)
文摘Excessive use of N fertilizers(driven by high-yield goals)and its consequent environmental problems are becoming increasingly acute in agricultural systems.A 2-year field experiment was conducted to investigate the effects of three N application methods(application of solid granular urea once(OF)or twice(TF),application of solid granular urea mixed with controlled-release urea once(MF),and six N rates(0,60,120,180,240,and 300 kg N ha^(-1))on maize yield,economic benefits,N use efficiency,and soil N balance in the maize(Zea mays L.)film mulching system on the Loess Plateau,China.The grain yield and economic return of maize were significantly affected by the N rate and application method.Compared with the OF treatment,the MF treatment not only increased the maize yield(increased by 9.0-16.7%)but also improved the economic return(increased by 10.9-25.8%).The agronomic N use efficiency(NAE),N partial factor productivity(NPFP)and recovery N efficiency(NRE)were significantly improved by 19.3-66.7,9.0-16.7 and 40.2-71.5%,respectively,compared with the OF treatment.The economic optimal N rate(EONR)of the OF,TF,and MF was 145.6,147.2,and 144.9 kg ha^(-1) in 2019,and 206.4,186.4,and 146.0 kg ha^(-1) in 2020,respectively.The apparent soil N loss at EONR of the OF,TF,and MF were 97.1-100.5,78.5-79.3,and 50.5-68.1 kg ha^(-1),respectively.These results support MF as a one-time N application method for delivering high yields and economic benefits,with low N input requirements within film mulching spring maize system on the Loess Plateau.