[Objective]The aim was to study the effects of regulation of C/N ratio wheat straw application on tobacco nitrogen,phosphorus and potassium uptake. [Method]Effects of regulation C/N ratio wheat straw application on th...[Objective]The aim was to study the effects of regulation of C/N ratio wheat straw application on tobacco nitrogen,phosphorus and potassium uptake. [Method]Effects of regulation C/N ratio wheat straw application on the flue-cured tobacco yield,output value,nitrogen,phosphorus and potassium content and cumulative uptake of the upper,middle and bottom leaf were studied by using the field plot experiments at Banqiao town,Qujing city,Yunnan Province during the 2008-2009 summer growing seasons. [Result]The results showed that the application of wheat straw alone or after C/N regulation,could significantly increase tobacco production,potassium content,the potassium and nitrogen accumulation amount of leaf,and was more conducive to the potassium uptake of tobacco leaf with wheat straw application after C/N regulation. Compared with non-straw application,the yield of tobacco increased by 6.59%,3.58%,5.98%,8.80% with application of wheat straw alone,wheat straw and vetch,wheat straw and oilseed cake,wheat straw and urea nitrogen,the potassium content in tobacco leaf increased by 3.85%,7.76%,8.82%,11.21%,respectively,the total potassium cumulative amount of leaf increased by 10.71%,11.62%,15.32% ,21.01% and the total nitrogen cumulative amount increased by 9.76%,1.22%,8.14%,14.00%. However,the differences of tobacco leaf nitrogen content among the different treatments were not significant,the phosphorus uptake of tobacco leaf decreased. [Conclusion]application of high C/N ratio wheat straw in flue-cured tobacco production,which should be concerned not only to adjust C/N ratio by adding nitrogen,but also considering additional phosphorus application.展开更多
Ectomycorrhizal fungus Laccaria bicolor S238N,isolated from a forest soil in central France in 1990s,has demonstrated unequivocally and ability to promote pine growth.In the present nursery bed experiment,the ability ...Ectomycorrhizal fungus Laccaria bicolor S238N,isolated from a forest soil in central France in 1990s,has demonstrated unequivocally and ability to promote pine growth.In the present nursery bed experiment,the ability of this ectomycorrhizal fungus to increase growth and P and K uptake of Douglas Fir seedlings (Zone 22) was examined.Growth of inoculated seedlings was over twice(plant height) and three times (biomass)that of non-inoculated ones.Similarly,both the concentrations and the amounts of P and K uptake by seedlings were significantly increased by fungal inoculation,indicating the improvement of P and K nutrition in mycorrhizal seedlings.In contrast,Al-P in the soils was decreased obviously by plants,especially by mycorrhizas,suggesting utilization of this soil P pool by plants and more efficient Al-P mobilization by mycorrhizas than by nomycorrhizas.Moreover,K extracted by 1mol/L HCl following consecutive extraction of H2O and CH3COONH4,which may not be plant available,could be utilized by fungus colonied roots.This could be explained by the release of protons and oxalate by hypae which leads to replacement of interlayer K in nonexpanded 2:1 clay minerals and bio-weathering of phyllosilicates.展开更多
The effects of varying nitrogen (N): phosphorus (P) ratios on the growth and N-uptake and assimilation of the harmful dinoflagellates Prorocentrum minimum and Prorocentrum donghaiense were examined in turbidistat...The effects of varying nitrogen (N): phosphorus (P) ratios on the growth and N-uptake and assimilation of the harmful dinoflagellates Prorocentrum minimum and Prorocentrum donghaiense were examined in turbidistat culture experiments. Algal cultures were supplied with media containing PO4^3- in various concentrations to obtain a wide range of N:P ratios. Experiments to determine rates of N uptake and assimilation of different N sources (NO^3-, NH4^+, urea and glycine by P. minimum and NO3^-, NH4^+ by P. donghaiense) were conducted using ^15-N tracer techniques at each N:P ratio. The growth rates suggested nutrient limitation at both high and low N:P ratios relative to the Redfield ratio. On a diel basis, the growth of both species was regulated by the light-dark cycle, which may be a result of regulation of both lightdependent growth and light-independent nutrient uptake. Maximum growth rates of both species always occurred at the beginning of light phase. In P-rich medium (low N:P ratio), both species had higher N assimilation rates, suggesting N limitation. Low assimilation coefficients at high N:P ratios suggested P limitation of N uptake and assimilation. NO3 ^-and NH4^+ contributed more than 90% of the total N uptake of P. minimum. Reduced N sources were more quickly assimilated than NO3^-. Highest average daily growth rates were recorded near an N:P ratio of 12 for both species. The N uptake rates of cultures at N:P ratios near Redfield ratio were more balanced with growth rates. The linkage between growth rates and N uptake/assimilation rates were conceptually described by the variation of cell N quota. The N:P ratios affect the N uptake and growth of Prorocentrum spp., and may regulate their bloom progression in eutrophic ecosystems.展开更多
The growth, chlorophyll composition, photosynthesis, respiration, K uptake and root K+ secretion offlue-cured tobacco as thected by different concentrations of N supplied as urea, NaNO3 and NH4NO3 werestudied under th...The growth, chlorophyll composition, photosynthesis, respiration, K uptake and root K+ secretion offlue-cured tobacco as thected by different concentrations of N supplied as urea, NaNO3 and NH4NO3 werestudied under the experimental condition of sand culture. The results showed that the content of K in thefiue-cured tobacco was not merely related with root vitality and uptake but also closely related with root cellmembrane structure and K+ secretion.展开更多
Two trials (1st Trial-46 and 2nd Trial-64 hrs duration) experiment in 8 out-door tanks (5 m3) were carried out with similar design: 4 fish densities (0, 1, 5, and 10), 2 densities of zooplankton (high and low) and 4 i...Two trials (1st Trial-46 and 2nd Trial-64 hrs duration) experiment in 8 out-door tanks (5 m3) were carried out with similar design: 4 fish densities (0, 1, 5, and 10), 2 densities of zooplankton (high and low) and 4 increasing levels of P and N concentrations of nutrient enrichments. The consecutive changes of N and P concentrations were measured at 3 (Trial 1) and 4 (Trial 2) time intervals. It was found that nutrient uptake of the entire community, which is the differences between initial and final concentrations, was mostly affected by the initial enrichment. The ecological implications are discussed.展开更多
Field and glasshouse experiments were conducted to study the effect of B. japonicum inoculation and phosphorus supplementation on macronutrient uptake by soybean. The treatments consisted of B. japonicum inoculation (...Field and glasshouse experiments were conducted to study the effect of B. japonicum inoculation and phosphorus supplementation on macronutrient uptake by soybean. The treatments consisted of B. japonicum inoculation (with & without), phosphorus supplementation at the levels of 0, 20, 40 and 80 kg P·ha-1. Both treatments were replicated four times in a split plot design. The macronutrients considered were N, P, K, Ca and Mg. Results showed that inoculation with B. japonicum significantly contribute to the uptake of N, P, K, Ca and Mg in roots, shoots, pods and the whole soybean plant. Likewise, phosphorus supplementation significantly enhanced the uptake of N, P, K, Ca and Mg in roots, shoots, pods and the whole plant. The use of effective strains of rhizobia and P supplementation was an effective way of enhancing the growth of soybean, eventually the uptake of macronutrients in plant organs.展开更多
Soybean (Glycine max (L.)) is one of the most important legume crops being introduced in the CHK (Central Highlands of Kenya) expected to increase yields. However, low levels of soil N (nitrogen) and other pla...Soybean (Glycine max (L.)) is one of the most important legume crops being introduced in the CHK (Central Highlands of Kenya) expected to increase yields. However, low levels of soil N (nitrogen) and other plant nutrients and soil acidity are seen as the major causes impairing goal achievement. To evaluate the influence of manure, lime, P (phosphorus) fertilizer and their combination on N uptake and soybean performance, an experiment was conducted in Embu ATC (Agricultural Training College) comprising 9 treatments, arranged in a Randomized Complete Block Design with 4 replicates in plots of 4 m×4.5 m. The study included manure (0, 5 and 10 t-ha-1), lime (0 and 2 t.ha-1) and P fertilizer (0, 30 and 60 kg.P.hal). The treatments significantly influenced N uptake and soybean yields. Both parameters responded well to application of manure both alone or combined to lime and TSP (triple super phosphate). From these it was concluded that organic and inorganic resources have potential to enhance N uptake and soybean and other crops yields in CHK.展开更多
In this paper,we investigate sufficient and necessary conditions such that generalized Forelli-Rudin type operators T_(λ,τ,k),S_(λ,τ,k),Q_(λ,τ,k)and R_(λ,τ,k)are bounded between Lebesgue type spaces.In order t...In this paper,we investigate sufficient and necessary conditions such that generalized Forelli-Rudin type operators T_(λ,τ,k),S_(λ,τ,k),Q_(λ,τ,k)and R_(λ,τ,k)are bounded between Lebesgue type spaces.In order to prove the main results,we first give some bidirectional estimates for several typical integrals.展开更多
Nitrogen(N)is one of the most limited nutrients of terrestrial ecosystems,whose losses are prevented in tightly coupled cycles in finely tuned systems.Global change-induced N enrichment through atmospheric deposition ...Nitrogen(N)is one of the most limited nutrients of terrestrial ecosystems,whose losses are prevented in tightly coupled cycles in finely tuned systems.Global change-induced N enrichment through atmospheric deposition and application of vast amounts of fertilizer are now challenging the terrestrial N cycle.Arbuscular mycorrhizal fungi(AMF)are known drivers of plant-soil nutrient fluxes,but a comprehensive assessment of AMF involvement in N cycling under global change is still lacking.Here,we simulated N enrichment by fertilization(low/high)in experimental grassland microcosms under greenhouse conditions in the presence or absence of AMF and continuously monitored different N pathways over nine months.We found that high N enrichment by fertilization decreased the relative abundance of legumes and the plant species dominating the plant community changed from grasses to forbs in the presence of AMF,based on aboveground biomass.The presence of AMF always maintained plant N:phosphorus(P)ratios between 14 and 16,no matter how the soil N availability changed.Shifts in plant N:P ratios due to the increased plant N and P uptake might thus be a primary pathway of AMF altering plant community composition.Furthermore,we constructed a comprehensive picture of AMF’s role in N cycling,highlighting that AMF reduced N losses primarily by mitigating N leaching,while N_(2)O emissions played a marginal role.Arbuscular mycorrhizal fungi reduced N_(2)O emissions directly through the promotion of N_(2)O-consuming denitrifiers.The underlying mechanism for reducing N leaching is mainly the AMF-mediated improved nutrient uptake and AMF-associated microbial immobilization.Our results indicate that synergies between AMF and other soil microorganisms cannot be ignored in N cycling and that the integral role of AMF in N cycling terrestrial ecosystems can buffer the upcoming global changes.展开更多
基金Supported by National Science and Technology Support Program(2006BAD05B06-04)~~
文摘[Objective]The aim was to study the effects of regulation of C/N ratio wheat straw application on tobacco nitrogen,phosphorus and potassium uptake. [Method]Effects of regulation C/N ratio wheat straw application on the flue-cured tobacco yield,output value,nitrogen,phosphorus and potassium content and cumulative uptake of the upper,middle and bottom leaf were studied by using the field plot experiments at Banqiao town,Qujing city,Yunnan Province during the 2008-2009 summer growing seasons. [Result]The results showed that the application of wheat straw alone or after C/N regulation,could significantly increase tobacco production,potassium content,the potassium and nitrogen accumulation amount of leaf,and was more conducive to the potassium uptake of tobacco leaf with wheat straw application after C/N regulation. Compared with non-straw application,the yield of tobacco increased by 6.59%,3.58%,5.98%,8.80% with application of wheat straw alone,wheat straw and vetch,wheat straw and oilseed cake,wheat straw and urea nitrogen,the potassium content in tobacco leaf increased by 3.85%,7.76%,8.82%,11.21%,respectively,the total potassium cumulative amount of leaf increased by 10.71%,11.62%,15.32% ,21.01% and the total nitrogen cumulative amount increased by 9.76%,1.22%,8.14%,14.00%. However,the differences of tobacco leaf nitrogen content among the different treatments were not significant,the phosphorus uptake of tobacco leaf decreased. [Conclusion]application of high C/N ratio wheat straw in flue-cured tobacco production,which should be concerned not only to adjust C/N ratio by adding nitrogen,but also considering additional phosphorus application.
文摘Ectomycorrhizal fungus Laccaria bicolor S238N,isolated from a forest soil in central France in 1990s,has demonstrated unequivocally and ability to promote pine growth.In the present nursery bed experiment,the ability of this ectomycorrhizal fungus to increase growth and P and K uptake of Douglas Fir seedlings (Zone 22) was examined.Growth of inoculated seedlings was over twice(plant height) and three times (biomass)that of non-inoculated ones.Similarly,both the concentrations and the amounts of P and K uptake by seedlings were significantly increased by fungal inoculation,indicating the improvement of P and K nutrition in mycorrhizal seedlings.In contrast,Al-P in the soils was decreased obviously by plants,especially by mycorrhizas,suggesting utilization of this soil P pool by plants and more efficient Al-P mobilization by mycorrhizas than by nomycorrhizas.Moreover,K extracted by 1mol/L HCl following consecutive extraction of H2O and CH3COONH4,which may not be plant available,could be utilized by fungus colonied roots.This could be explained by the release of protons and oxalate by hypae which leads to replacement of interlayer K in nonexpanded 2:1 clay minerals and bio-weathering of phyllosilicates.
基金supported by the University of Maryland Center for Environmental Science (UMCES), Horn Point Laboratory. This is UMCES contribution number 4503
文摘The effects of varying nitrogen (N): phosphorus (P) ratios on the growth and N-uptake and assimilation of the harmful dinoflagellates Prorocentrum minimum and Prorocentrum donghaiense were examined in turbidistat culture experiments. Algal cultures were supplied with media containing PO4^3- in various concentrations to obtain a wide range of N:P ratios. Experiments to determine rates of N uptake and assimilation of different N sources (NO^3-, NH4^+, urea and glycine by P. minimum and NO3^-, NH4^+ by P. donghaiense) were conducted using ^15-N tracer techniques at each N:P ratio. The growth rates suggested nutrient limitation at both high and low N:P ratios relative to the Redfield ratio. On a diel basis, the growth of both species was regulated by the light-dark cycle, which may be a result of regulation of both lightdependent growth and light-independent nutrient uptake. Maximum growth rates of both species always occurred at the beginning of light phase. In P-rich medium (low N:P ratio), both species had higher N assimilation rates, suggesting N limitation. Low assimilation coefficients at high N:P ratios suggested P limitation of N uptake and assimilation. NO3 ^-and NH4^+ contributed more than 90% of the total N uptake of P. minimum. Reduced N sources were more quickly assimilated than NO3^-. Highest average daily growth rates were recorded near an N:P ratio of 12 for both species. The N uptake rates of cultures at N:P ratios near Redfield ratio were more balanced with growth rates. The linkage between growth rates and N uptake/assimilation rates were conceptually described by the variation of cell N quota. The N:P ratios affect the N uptake and growth of Prorocentrum spp., and may regulate their bloom progression in eutrophic ecosystems.
文摘The growth, chlorophyll composition, photosynthesis, respiration, K uptake and root K+ secretion offlue-cured tobacco as thected by different concentrations of N supplied as urea, NaNO3 and NH4NO3 werestudied under the experimental condition of sand culture. The results showed that the content of K in thefiue-cured tobacco was not merely related with root vitality and uptake but also closely related with root cellmembrane structure and K+ secretion.
文摘Two trials (1st Trial-46 and 2nd Trial-64 hrs duration) experiment in 8 out-door tanks (5 m3) were carried out with similar design: 4 fish densities (0, 1, 5, and 10), 2 densities of zooplankton (high and low) and 4 increasing levels of P and N concentrations of nutrient enrichments. The consecutive changes of N and P concentrations were measured at 3 (Trial 1) and 4 (Trial 2) time intervals. It was found that nutrient uptake of the entire community, which is the differences between initial and final concentrations, was mostly affected by the initial enrichment. The ecological implications are discussed.
文摘Field and glasshouse experiments were conducted to study the effect of B. japonicum inoculation and phosphorus supplementation on macronutrient uptake by soybean. The treatments consisted of B. japonicum inoculation (with & without), phosphorus supplementation at the levels of 0, 20, 40 and 80 kg P·ha-1. Both treatments were replicated four times in a split plot design. The macronutrients considered were N, P, K, Ca and Mg. Results showed that inoculation with B. japonicum significantly contribute to the uptake of N, P, K, Ca and Mg in roots, shoots, pods and the whole soybean plant. Likewise, phosphorus supplementation significantly enhanced the uptake of N, P, K, Ca and Mg in roots, shoots, pods and the whole plant. The use of effective strains of rhizobia and P supplementation was an effective way of enhancing the growth of soybean, eventually the uptake of macronutrients in plant organs.
文摘Soybean (Glycine max (L.)) is one of the most important legume crops being introduced in the CHK (Central Highlands of Kenya) expected to increase yields. However, low levels of soil N (nitrogen) and other plant nutrients and soil acidity are seen as the major causes impairing goal achievement. To evaluate the influence of manure, lime, P (phosphorus) fertilizer and their combination on N uptake and soybean performance, an experiment was conducted in Embu ATC (Agricultural Training College) comprising 9 treatments, arranged in a Randomized Complete Block Design with 4 replicates in plots of 4 m×4.5 m. The study included manure (0, 5 and 10 t-ha-1), lime (0 and 2 t.ha-1) and P fertilizer (0, 30 and 60 kg.P.hal). The treatments significantly influenced N uptake and soybean yields. Both parameters responded well to application of manure both alone or combined to lime and TSP (triple super phosphate). From these it was concluded that organic and inorganic resources have potential to enhance N uptake and soybean and other crops yields in CHK.
基金supported by the Natural Science Foundation of Hunan Province of China(2022JJ30369)the Education Department Important Foundation of Hunan Province in China(23A0095)。
文摘In this paper,we investigate sufficient and necessary conditions such that generalized Forelli-Rudin type operators T_(λ,τ,k),S_(λ,τ,k),Q_(λ,τ,k)and R_(λ,τ,k)are bounded between Lebesgue type spaces.In order to prove the main results,we first give some bidirectional estimates for several typical integrals.
基金supported by the National Natural Science Foundation of China(Nos.32101304 and 32160281)the Key Laboratory Project,Xinjiang,China(No.2021D04006)+1 种基金China Postdoctoral Science Foundation(No.2021M692707)supported by the Swiss National Science Foundation(No.31003A-166079)。
文摘Nitrogen(N)is one of the most limited nutrients of terrestrial ecosystems,whose losses are prevented in tightly coupled cycles in finely tuned systems.Global change-induced N enrichment through atmospheric deposition and application of vast amounts of fertilizer are now challenging the terrestrial N cycle.Arbuscular mycorrhizal fungi(AMF)are known drivers of plant-soil nutrient fluxes,but a comprehensive assessment of AMF involvement in N cycling under global change is still lacking.Here,we simulated N enrichment by fertilization(low/high)in experimental grassland microcosms under greenhouse conditions in the presence or absence of AMF and continuously monitored different N pathways over nine months.We found that high N enrichment by fertilization decreased the relative abundance of legumes and the plant species dominating the plant community changed from grasses to forbs in the presence of AMF,based on aboveground biomass.The presence of AMF always maintained plant N:phosphorus(P)ratios between 14 and 16,no matter how the soil N availability changed.Shifts in plant N:P ratios due to the increased plant N and P uptake might thus be a primary pathway of AMF altering plant community composition.Furthermore,we constructed a comprehensive picture of AMF’s role in N cycling,highlighting that AMF reduced N losses primarily by mitigating N leaching,while N_(2)O emissions played a marginal role.Arbuscular mycorrhizal fungi reduced N_(2)O emissions directly through the promotion of N_(2)O-consuming denitrifiers.The underlying mechanism for reducing N leaching is mainly the AMF-mediated improved nutrient uptake and AMF-associated microbial immobilization.Our results indicate that synergies between AMF and other soil microorganisms cannot be ignored in N cycling and that the integral role of AMF in N cycling terrestrial ecosystems can buffer the upcoming global changes.