Nucleotide-binding site leucine-rich repeat receptors (NBS-LRR/NLRs) are crucial intracellular immune proteins in plants. Previous article reported a novel NLR protein SUT1 (SUPPRESSORS OF TOPP4-1, 1), which is involv...Nucleotide-binding site leucine-rich repeat receptors (NBS-LRR/NLRs) are crucial intracellular immune proteins in plants. Previous article reported a novel NLR protein SUT1 (SUPPRESSORS OF TOPP4-1, 1), which is involved in autoimmunity initiated by type one protein phosphatase 4 mutation (topp4-1) in Arabidopsis, however, its role in planta is still unclear. This study employed Nicotiana benthamiana, a model platform, to conduct an overall structural and functional analysis of SUT1 protein. The transient expression results revealed that SUT1 is a typical CNL (CC-NBS-LRR) receptor, both fluorescence data and biochemical results showed the protein is mainly anchored on the plasma membrane due to its N-terminal acylation site. Further truncation experiments announced that its CC (coiled-coil) domain possessed cell-death-inducing activity. The outcomes of point mutations analysis revealed that not only the CC domain, but also the full-length SUT1 protein, whose function and subcellular localization are influenced by highly conserved hydrophobic residues. These research outcomes provided favorable clues for elucidating the activation mechanism of SUT1.展开更多
全球气候变化加重了沿海地区的气候暴露度,基于自然的解决方案(NbS)理念的活体海岸线(living shoreline)展示了更具韧性、更环境友好的海岸保护路径,但其相关基本理论和全生命周期管理框架尚未得到系统梳理。查阅国外相关部门项目公报及...全球气候变化加重了沿海地区的气候暴露度,基于自然的解决方案(NbS)理念的活体海岸线(living shoreline)展示了更具韧性、更环境友好的海岸保护路径,但其相关基本理论和全生命周期管理框架尚未得到系统梳理。查阅国外相关部门项目公报及Web of Science数据库,梳理近10年活体海岸线的相关研究与实践,从活体海岸线的分析规划、构造设计、施工建造、监测评估4个阶段进行总结分析,并结合实践应用和中国国情,客观看待目前活体海岸线的研究局限性;将其与传统硬质工程护岸进行辨证性的比较和思考,提出完善全生命周期管理的框架建议,旨在为我国的海岸保护与生境修复工作提供参考。展开更多
文摘Nucleotide-binding site leucine-rich repeat receptors (NBS-LRR/NLRs) are crucial intracellular immune proteins in plants. Previous article reported a novel NLR protein SUT1 (SUPPRESSORS OF TOPP4-1, 1), which is involved in autoimmunity initiated by type one protein phosphatase 4 mutation (topp4-1) in Arabidopsis, however, its role in planta is still unclear. This study employed Nicotiana benthamiana, a model platform, to conduct an overall structural and functional analysis of SUT1 protein. The transient expression results revealed that SUT1 is a typical CNL (CC-NBS-LRR) receptor, both fluorescence data and biochemical results showed the protein is mainly anchored on the plasma membrane due to its N-terminal acylation site. Further truncation experiments announced that its CC (coiled-coil) domain possessed cell-death-inducing activity. The outcomes of point mutations analysis revealed that not only the CC domain, but also the full-length SUT1 protein, whose function and subcellular localization are influenced by highly conserved hydrophobic residues. These research outcomes provided favorable clues for elucidating the activation mechanism of SUT1.
文摘全球气候变化加重了沿海地区的气候暴露度,基于自然的解决方案(NbS)理念的活体海岸线(living shoreline)展示了更具韧性、更环境友好的海岸保护路径,但其相关基本理论和全生命周期管理框架尚未得到系统梳理。查阅国外相关部门项目公报及Web of Science数据库,梳理近10年活体海岸线的相关研究与实践,从活体海岸线的分析规划、构造设计、施工建造、监测评估4个阶段进行总结分析,并结合实践应用和中国国情,客观看待目前活体海岸线的研究局限性;将其与传统硬质工程护岸进行辨证性的比较和思考,提出完善全生命周期管理的框架建议,旨在为我国的海岸保护与生境修复工作提供参考。
