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熊果酸干预人牙周膜干细胞成骨分化的效应 被引量:1
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作者 郑茜 刘萍萍 +1 位作者 顾煜婕 谢蕾 《中国组织工程研究》 CAS 北大核心 2025年第1期80-86,共7页
背景:熊果酸可促进骨髓间充质干细胞向成骨细胞定向分化,但其对人牙周膜干细胞是否具有促成骨作用目前相关报道少见。目的:探讨熊果酸对人牙周膜干细胞增殖和成骨分化能力的影响。方法:分离、培养人牙周膜干细胞,取第3代细胞,分别采用... 背景:熊果酸可促进骨髓间充质干细胞向成骨细胞定向分化,但其对人牙周膜干细胞是否具有促成骨作用目前相关报道少见。目的:探讨熊果酸对人牙周膜干细胞增殖和成骨分化能力的影响。方法:分离、培养人牙周膜干细胞,取第3代细胞,分别采用含不同浓度(0,1,2,4,6,8μmol/L)熊果酸的普通培养基干预,干预1,3,5,7 d后,采用CCK-8法检测细胞增殖,筛选适宜干预浓度。取第3代人牙周膜干细胞,分别用含0,1,2,4μmol/L熊果酸的成骨诱导液干预,干预7 d后,采用qRT-PCR法检测碱性磷酸酶、Runx2、骨钙素m RNA的表达,干预14 d后,茜素红染色观察矿化结节形成。取第3代人牙周膜干细胞,对照组加入成骨诱导液,熊果酸组、拮抗剂组分别加入含熊果酸(2μmol/L)、骨形态发生蛋白信号通路拮抗剂Noggin的成骨诱导液,熊果酸+拮抗剂组加入含熊果酸(2μmol/L)、骨形态发生蛋白信号通路抑制剂Noggin的成骨诱导液,培养7 d后,采用qRT-PCR和Western blot检测骨形态发生蛋白2、Smad1、骨桥蛋白、Runx2的m RNA及蛋白表达。结果与结论:(1)1,2,4μmol/L熊果酸可促进人牙周膜干细胞的增殖,6,8μmol/L熊果酸可抑制人牙周膜干细胞的增殖,后续实验选择1,2,4μmol/L熊果酸进行干预;(2)与0μmol/L相比,1,2,4μmol/L熊果酸可促进碱性磷酸酶、Runx2、骨钙素m RNA的表达以及矿化结节的形成(P<0.05),其中以2μmol/L熊果酸效果最显著;(3)与对照组比较,熊果酸组骨形态发生蛋白2、Smad1、骨桥蛋白、Runx2的mRNA及蛋白表达均升高(P<0.05),拮抗剂组上述指标的mRNA及蛋白表达均降低(P<0.05);与熊果酸组比较,熊果酸+拮抗剂组上述指标的mRNA及蛋白表达均降低(P<0.05);(4)结果表明,熊果酸可通过激活骨形态发生蛋白信号通路促进人牙周膜干细胞的成骨分化。 展开更多
关键词 人牙周膜干细胞 熊果酸 成骨 骨形态发生蛋白信号通路 碱性磷酸酶 骨钙素 骨形态发生蛋白2 Smad1蛋白
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Osteocalcin as a hormone regulating glucose metabolism 被引量:5
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作者 Ippei Kanazawa 《World Journal of Diabetes》 SCIE CAS 2015年第18期1345-1354,共10页
The number of patients with osteoporosis and diabetes is rapidly increasing all over the world. Bone is recently recognized as an endocrine organ. Accumulating evidence has shown that osteocalcin, which is specificall... The number of patients with osteoporosis and diabetes is rapidly increasing all over the world. Bone is recently recognized as an endocrine organ. Accumulating evidence has shown that osteocalcin, which is specifically expressed in osteoblasts and secreted into the circulation, regulates glucose homeostasis by stimulating insulin expression in pancreas and adiponectin expression in adipocytes, resulting in improving glucose intolerance. On the other hand, insulin and adiponectin stimulate osteocalcin expression in osteoblasts, suggesting that positive feedforward loops exist among bone, pancreas, and adipose tissue. In addition, recent studies have shown that osteocalcin enhances insulin sensitivity and the differentiation in muscle, while secreted factors from muscle, myokines, regulate bone metabolism. These findings suggest that bone metabolism and glucose metabolism are associated with each other through the action of osteocalcin. In this review, I describe the role of osteocalcin in the interaction among bone, pancreas, brain, adipose tissue, and muscle. 展开更多
关键词 osteocalcin Undercarboxylated osteocalcin Glucose INSULIN ADIPONECTIN Glucagon-like peptide-1 Diabetes MELLITUS
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Overexpression of Dlx2 enhances osteogenic differentiation of BMSCs and MC3T3-E1 cells via direct upregulation of Osteocalcin and Alp 被引量:21
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作者 Jianfei Zhang Wenbin Zhang +2 位作者 Jiewen Dai Xudong Wang Steve Guofang Shen 《International Journal of Oral Science》 SCIE CAS CSCD 2019年第2期119-130,共12页
Genetic studies have revealed a critical role of Distal-homeobox (Dlx) genes in bone formation,and our previous study showed that Dlx2 overexpressing in neural crest cells leads to profound abnormalities of the cranio... Genetic studies have revealed a critical role of Distal-homeobox (Dlx) genes in bone formation,and our previous study showed that Dlx2 overexpressing in neural crest cells leads to profound abnormalities of the craniofacial tissues.The aim of this study was to investigate the role and the underlying molecular mechanisms of Dlx2 in osteogenic differentiation of mouse bone marrow stromal cells (BMSCs) and pre-osteoblast MC3T3-E1 cells.Initially,we observed upregulation of Dlx2 during the early osteogenesis in BMSCs and MC3T3-E1 cells.Moreover,Dlx2 overexpression enhanced alkaline phosphatase (ALP) activity and extracellular matrix mineralization in BMSCs and MC3T3-E1 cell line.In addition,micro-CT of implanted tissues in nude mice confirmed that Dlx2 overexpression in BMSCs promoted bone formation in vivo.Unexpectedly,Dlx2 overexpression had little impact on the expression level of the pivotal osteogenic transcription factors Runx2,Dlx5,Msx2,and Osterix,but led to upregulation of Alp and Osteocalcin (OCN),both of which play critical roles in promoting osteoblast maturation.Importantly,luciferase analysis showed that Dlx2 overexpression stimulated both OCN and Alp promoter activity.Through chromatin-immunoprecipitation assay and site-directed mutagenesis analysis,we provide molecular evidence that Dlx2 transactivates OCN and Alp expression by directly binding to the Dlx2-response cis-acting elements in the promoter of the two genes.Based on these findings,we demonstrate that Dlx2 overexpression enhances osteogenic differentiation in vitro and accelerates bone formation in vivo via direct upregulation of the OCN and Alp gene,suggesting that Dlx2 plays a crucial role in osteogenic differentiation and bone formation. 展开更多
关键词 BMSCS MC3T3-E1 osteocalcin
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Serum levels of undercarboxylated osteocalcin are related to cardiovascular risk factors in patients with type 2 diabetes mellitus and healthy subjects 被引量:6
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作者 Sergio Sanchez-Enriquez Isabel Thalia Ballesteros-Gonzalez +8 位作者 JoséRafael Villafán-Bernal Sara Pascoe-Gonzalez Edgar Alfonso Rivera-Leon Blanca Estela Bastidas-Ramirez Jorge David Rivas-Carrillo Juan Luis Alcala-Zermeno Juan Armendariz-Borunda Iris Monserrat Llamas-Covarrubias Abraham Zepeda-Moreno 《World Journal of Diabetes》 SCIE CAS 2017年第1期11-17,共7页
AIM To determine a potential relationship between serum undercarboxylated(uc OC) concentration and cardiovascular risk factors in type 2 diabetes(T2D) patients and healthy subjects(HS).METHODS A cross-sectional study ... AIM To determine a potential relationship between serum undercarboxylated(uc OC) concentration and cardiovascular risk factors in type 2 diabetes(T2D) patients and healthy subjects(HS).METHODS A cross-sectional study was conducted on 140 subjects classified into two groups, 70 with T2D and 70 HS. Medical history and physical examination with anthropometric measurements were obtained from all subjects. Body fat percentage was determined by bioelectrical impendency analysis. Serum uc OC concentration was determined by enzyme immunoassay,while serum levels of insulin and hsC RP were obtained using high sensitivity enzyme-linked immunosorbent assay. Insulin resistance was determined using the homeostasis model assessment-IR. Lipid profile [triglycerides,total cholesterol(TC), high-density lipoproteins(HDL-c),low density lipoproteins(LDL-c), very low-density lipoproteins] was determined by spectrophotometry and standard formulas when applicable. RESULTS The T2D patient group showed significantly higher values of waist circumference, waist-to-hip ratio, systolic blood pressure(SBP), diastolic blood pressure(DBP),current smoking, and alcohol use when compared to the HS group(P < 0.05). We observed a significantly lower serum ucO C concentration in T2D than in HS(1.5 ± 1.4vs 2.3 ± 1.8, P < 0.05). In the whole study population,ucO C concentration was inversely correlated with body mass index(BMI)(r =-0.236, P < 0.05), fasting plasma glucose(r =-0.283, P < 0.01) and HDL-c(r =-0.255,P < 0.05); and positively correlated with LDL-c/HDL-c ratio(r = 0.306, P < 0.05) and TC/HDL-c ratio(r =0.284, P < 0.05). In the T2D group, serum uc OC concentration was inversely correlated with BMI(r =-0.310, P < 0.05) and body-fat percentage(r =-0.311,P < 0.05), and positively correlated with DBP(r = 0.450,P < 0.01). In HS group a positive correlation between serum levels of uc OC and SBP(r = 0.277, P < 0.05)was observed. CONCLUSION Serum uc OC is a potential marker for cardiovascular risk in Mexicans because it is related to adiposity parameters, blood pressure and lipid profile. 展开更多
关键词 BONE osteocalcin Glucose metabolism DIABETES Cardiovascular risk
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Decarboxylated osteocalcin,a possible drug for type 2 diabetes,triggers glucose uptake in MG63 cells 被引量:1
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作者 Shi Jin Xiao-Cen Chang +5 位作者 Jing Wen Jing Yang Na Ao Ke-Ying Zhang Lin-Na Suo Jian Du 《World Journal of Diabetes》 SCIE 2021年第7期1102-1115,共14页
BACKGROUND Uncarboxylated osteocalcin(GluOC)has been reported to improve glucose metabolism,prevent type 2 diabetes,and decrease the severity of obesity in mice with type 2 diabetes.GluOC can increase glucose uptake i... BACKGROUND Uncarboxylated osteocalcin(GluOC)has been reported to improve glucose metabolism,prevent type 2 diabetes,and decrease the severity of obesity in mice with type 2 diabetes.GluOC can increase glucose uptake in a variety of cells.Glucose metabolism is the main source of energy for osteoblast proliferation and differentiation.We hypothesized that decarboxylated osteocalcin(dcOC),a kind of GluOC,can increase glucose uptake in MG63 cells(osteoblast-like osteosarcoma cells)and influence their proliferation and differentiation.AIM To investigate the effects of dcOC on glucose uptake in human osteoblast-like osteosarcoma cells and the possible signaling pathways involved.METHODS MG63 cells(human osteoblast-like osteosarcoma cells)were treated with dcOC(0,0.3,3,10,or 30 ng/mL)for 1 and 72 h,and glucose uptake was measured by flow cytometry.The effect of dcOC on cell proliferation was measured with a CCK-8 assay,and alkaline phosphatase(ALP)enzyme activity was measured.PI3K was inhibited with LY294002,and hypoxia-inducible factor 1 alpha(HIF-1α)was silenced with siRNA.Then,GPRC6A(G protein-coupled receptor family C group 6 subtype A),total Akt,phosphorylated Akt,HIF-1α,and glucose transporter 1(GLUT1)levels were measured by Western blot to elucidate the possible pathways by which dcOC modulates glucose uptake.RESULTS The glucose uptake of MG63 cells was significantly increased compared with that of the paired control cells after short-term(1 h)treatment with dcOC at different concentrations(0.3,3,and 10 ng/mL groups,P<0.01;30 ng/mL group,P<0.05).Glucose uptake of MG63 cells was significantly increased compared with that of the paired control cells after long-term(72 h)treatment with dcOC at different concentrations(0.3,3,and 10 ng/mL groups,P<0.01;30 ng/mL group,P<0.05).DcOC triggered Akt phosphorylation in a dose-dependent manner,and the most effective stimulatory concentration of dcOC for short-term(1 h)was 3 ng/mL(P<0.01).LY294002 abolished the dcOC-mediated(1 h)promotion of Akt phosphorylation and glucose uptake without affecting GLUT1 protein expression.Long-term dcOC stimulation triggered Akt phosphorylation and increased the protein levels of HIF-1α,GLUT1,and Runx2 in a dose-dependent manner.Inhibition of HIF-1αwith siRNA abolished the dcOC-mediated glucose uptake and substantially decreased GLUT1 protein expression.DcOC interven-tion promoted cell proliferation in a time-and dose-dependent manner as determined by the CCK-8 assay.Treatment with both 3 ng/mL and 10 ng/mL dcOC affected the ALP activity in MG63 cells after 72 h(P<0.01).CONCLUSION Short-and long-term dcOC treatment can increase glucose uptake and affect proliferation and ALP activity in MG63 cells.This effect may occur through the PI3K/Akt,HIF-1α,and GLUT1 signaling factors. 展开更多
关键词 Decarboxylated osteocalcin OSTEOBLAST Glucose uptake Glucose transporter 1 Type 2 diabetes
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Effects of β-TCP Ceramics on Osteoblast Cellular Proliferating,Mineralization and Osteocalcin Expression
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作者 QI Zhitao ZHANG Qihuan +4 位作者 ZHENG Qiang DAI Honglian WANG Zisheng QIU Ming LI Shipu 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS 2012年第1期107-109,共3页
After co-cultrured osteoblast with fl-TCP ceramics, the cellular proliferating, mineralization and osteocalcin expression were studied. MTT assay showed that fl-TCP ceramics had no affect on cellular proliferating. La... After co-cultrured osteoblast with fl-TCP ceramics, the cellular proliferating, mineralization and osteocalcin expression were studied. MTT assay showed that fl-TCP ceramics had no affect on cellular proliferating. Laser scanning confocal detection showed that fl-TCP ceramics could increase the mineralization level of osteoblast. Furthermore, RT-PCR showed that fl-TCP could increase the expression level of osteocalcin. Those results indicate β-TCP ceramics had perfect biocompatibility and increased the mineralization of osteoblast to accelerate osteogenesis by means of affecting the expression of genes involving in osteogeneticprocess. 展开更多
关键词 β-TCP ceramics OSTEOBLAST cellular proliferating MINERALIZATION osteocalcin
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RU486 Reversal of Cortisol Repression of 1,25-Dihydroxyvitamin D<sub>3</sub>Induction of the Human Osteocalcin Promoter
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作者 Nigel A. Morrison 《Open Journal of Endocrine and Metabolic Diseases》 2013年第1期55-62,共8页
In conditions of corticosteroid excess, such as Cushing’s syndrome, a reduction in serum osteocalcin is observed and bone loss occurs. The human osteocalcin gene is induced by 1,25-dihydroxyvitamin D3 derivatives and... In conditions of corticosteroid excess, such as Cushing’s syndrome, a reduction in serum osteocalcin is observed and bone loss occurs. The human osteocalcin gene is induced by 1,25-dihydroxyvitamin D3 derivatives and repressed by glucocorticoids. In this paper we show that cortisol, a natural glucocorticoid, represses both basal and vitamin D induced activity of the human osteocalcin promoter. Furthermore, we address the specific question as to whether the anti-progestin anti-glucocorticoid RU486 is able to antagonize the inhibitory effect of cortisol on osteocalcin gene expression. We show that RU486 has agonist activity alone, in that it is able to repress the basal promoter activity of the osteocalcin gene and antagonist activity, reversing incompletely the cortisol mediated repression of 1,25-dihydroxyvitamin D3 induction. 展开更多
关键词 RU486 osteocalcin Vitamin D Glucocorticoid
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Skeletal events of Anastrozole versus Tamoxifen on bone mineral density and bone biomarker osteocalcin in postmenopausal women with early breast cancer
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作者 Lobna R Ezz Elarab Menha Swellam +1 位作者 Manal M Abdel Wahab Karima M Maher 《The Chinese-German Journal of Clinical Oncology》 CAS 2010年第10期574-578,共5页
Objective: Postmenopausal women with breast cancer are at increased risk of bone loss because of age related estrogen deficiency face which accelerated with the use of aromatase inhibitors (AIs). We aimed to study ... Objective: Postmenopausal women with breast cancer are at increased risk of bone loss because of age related estrogen deficiency face which accelerated with the use of aromatase inhibitors (AIs). We aimed to study the effect on bone mineral density (BMD) and bone formation biomarker osteocalcin level in postmenopausal breast cancer patients, for the first three years of adjuvant hormonal treatment of both groups Tamoxifen versus Anastrozol. Methods: One-hundered postmenopausal breast cancers were prospectively randomized to receive either Tamoxifen 20 rag/day (n = 50) or Anastrozole 10 mg (n = 50). Both BMD and osteocalcin were assessed initially before treatment and then at regular intervals for both groups. Results: Use of Tamoxifen was associated with significant annual decrease in osteocalcin (P = 0.001), whereas Anastrozole group had gradual increase of the annual levels (P 〈 0.01). BMD decreased significantly in Anastrozole versus Tamoxifen groups (2.6% vs. 0.4%, P 〈 0.001). Osteoporosis T 〈 -2.5 was reported significantly higher in Anastrozole group (P 〈 0.01). Women with initial osteopenia in Anastrozole group showed significant decrease in BMD (P 〈 0.05). The addition of bisphosphonate for patients with early osteoporosis markedly improved both osteocalcin level and BMD. Conclusion: Tamoxifen preserves BMD in postmenopausal breast cancer patients, whereas Anastrozole accelerates age associated fall in BMD especially in the first year of therapy, moreover, the addition of bisphosphonate can help to decrease the skeletal related events associated with treatment to ensure better quality of life with treatment. 展开更多
关键词 ANASTROZOLE Tamoxifen bone mineral density (BMD) breast cancer osteocalcin
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Relationship between serum osteocalcin level and glucose and lipid metabolism in patients with type 2 diabetes mellitus complicated with nonalcoholic fatty liver disease
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作者 Ying Shen Lei-Qun Lu Ping Gao 《Journal of Hainan Medical University》 2019年第9期20-23,共4页
Objective: To study the relationship between serum osteocalcin level and glucose and lipid metabolism in patients with type 2 diabetes mellitus complicated with nonalcoholic fatty liver disease. Methods: The clinical ... Objective: To study the relationship between serum osteocalcin level and glucose and lipid metabolism in patients with type 2 diabetes mellitus complicated with nonalcoholic fatty liver disease. Methods: The clinical data of 180 type 2 diabetes mellitus patients in Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, China from February 2017 to January 2018 were retrospectively analyzed, including 90 cases of nonalcoholic fatty liver disease patients (group A) and 90 cases of nonalcoholic fatty liver disease-free patients (group B), meanwhile another 100 healthy subjects were selected as the control group. Then various indexes were compared between groups, including serum osteocalcin, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), prothrombin activity (PTA), fasting blood glucose (FBG), glycated hemoglobin (HbA1c), triglyceride (TG), total cholesterol (TC), low density lipoprotein (LDL), high density lipoprotein (HDL), fasting insulin (FINS), fasting C peptide (FCP), HOMA insulin resistance index (HOMA-IR), HOMA β-cell function (HOMA-β). Results: The serum osteocalcin and PTA in group A were significantly lower than those in group B and the control group (P<0.05). ALT, AST, and ALP were significantly higher than those in group B and the control group (P<0.05). The FBG and HbA1c in group A were significantly higher than those in group B and the control group (P<0.05). The TG, TC, LDL, and HDL of group A and group B were significantly higher than those of the control group (P<0.05). The FINS, FCP, and HOMA-IR in group A were significantly higher than those in group B and the control group (P<0.05). HOMA-βwas significantly lower than group B and the control group (P<0.05). Pearson correlation analysis showed that the serum osteocalcin was not correlated with ALT, AST, ALP, PTA, HbA1c, TG, TC, LDL, HDL and FINS (P>0.05), but negatively correlated with FBG and HOMA-IR (P<0.05), and positively correlated with FCP and HOMA-β (P<0.05). With serum osteocalcin as the dependent variable, and ALT, AST, ALP, PTA, FBG, HbA1c, TG, TC, LDL, HDL, FINS, FCP, HOMA-IR and HOMA-β as independent variables, multiple stepwise regression analysis showed that the FBG, HOMA-IR and HOMA-β were independent risk factors for osteocalcin. Conclusions: Patients with type 2 diabetes mellitus complicated with nonalcoholic fatty liver disease have lower serum osteocalcin level, which is susceptible to FBG, HOMA-IR, HOMA-β, and other factors. 展开更多
关键词 Type 2 diabetes MELLITUS NONALCOHOLIC FATTY liver disease Serum osteocalcin GLYCOLIPID metabolism
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外敷温经药酒联合针刺对骨质疏松大鼠的影响
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作者 温志刚 马欣 +3 位作者 路帅 孙晓飞 董军格 刘三豹 《中国骨质疏松杂志》 CAS CSCD 北大核心 2024年第9期1305-1310,共6页
目的探究外敷温经药酒联合针刺对骨质疏松大鼠的影响。方法选取SPF级雌性大鼠40只,分为正常组、模型组、针刺组、联合组,每组10只。除正常组外其余均建立骨质疏松(osteoporosis,OP)大鼠模型。建模成功后正常组、模型组不予处理,针刺组... 目的探究外敷温经药酒联合针刺对骨质疏松大鼠的影响。方法选取SPF级雌性大鼠40只,分为正常组、模型组、针刺组、联合组,每组10只。除正常组外其余均建立骨质疏松(osteoporosis,OP)大鼠模型。建模成功后正常组、模型组不予处理,针刺组入穴关元、三阴交(双)、肾俞(双)、后三里(双)后连接电针仪治疗,5 d/周,每日一次,20 min/次,共12周。联合组在针刺治疗基础上外敷温经药酒,每日两次,共12周。用ELISA法检测血清中骨钙素(OC)、吡啶酚(PYD)、Ⅰ型前胶原羧基端肽(PICP)水平;用计算机断层扫描检测骨小梁厚度(Tb.Th)、骨小梁间隔(Tb.Sp)、骨密度(bone mineral density,BMD)、皮质骨面积(Ct.Ar);运用TUNEL法检测成骨细胞凋亡;通过HE染色观察股骨组织病理改变;通过Western blot法测Notch1、Notch2、Jagged1、Jagged2。结果与正常组比较,模型组PYD、Tb.Sp、成骨细胞凋亡率升高,OC、PICP、Tb.Th、BMD、Ct.A、Notch1、Notch2、Jagged1、Jagged2降低(P<0.05);与模型组比较,针刺组PYD、Tb.Sp、成骨细胞凋亡率降低,OC、PICP、Tb.Th、BMD、Ct.A、Notch1、Notch2、Jagged1、Jagged2升高(P<0.05);与针刺组比较,联合组PYD、Tb.Sp、成骨细胞凋亡率降低,OC、PICP、Tb.Th、BMD、Ct.A、Notch1、Notch2、Jagged1、Jagged2升高(P<0.05);正常组大鼠骨形态结构良好;模型组骨损伤严重;针刺组与联合组骨形态结构有所改善,其中联合组改善更为良好。结论外敷温经药酒联合针刺对骨质疏松大鼠的骨钙素、成骨细胞活性及Notch信号通路等的改善作用良好,建议推广使用。 展开更多
关键词 骨质疏松 温经药酒 针刺 骨钙素 成骨细胞
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血清骨钙素及SIRT6在急性缺血性脑卒中的表达及预后价值分析
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作者 何德英 潘成德 +1 位作者 郭富饶 江思德 《疑难病杂志》 CAS 2024年第4期435-439,共5页
目的分析急性缺血性脑卒中(AIS)患者血清骨钙素、去乙酰化酶6(SIRT6)表达及其对临床预后的评估价值。方法收集2019年2月—2021年2月重庆市巴南区人民医院神经内科诊治AIS患者90例为AIS组,以同期诊治的高血压或糖尿病患者90例为非AIS组,... 目的分析急性缺血性脑卒中(AIS)患者血清骨钙素、去乙酰化酶6(SIRT6)表达及其对临床预后的评估价值。方法收集2019年2月—2021年2月重庆市巴南区人民医院神经内科诊治AIS患者90例为AIS组,以同期诊治的高血压或糖尿病患者90例为非AIS组,以同期体检的健康人90例为健康对照组。随访3个月,根据AIS患者预后分为预后不良亚组(n=24)和预后良好亚组(n=66)。利用酶联免疫吸附试验检测血清骨钙素及SIRT6水平。分析AIS患者预后影响因素及血清骨钙素、SIRT6对AIS患者预后评估价值。结果与健康对照组比较,AIS组血清骨钙素、SIRT6水平显著降低(t/P=25.013/<0.001、27.571/<0.001),与非AIS组比较,AIS组显著降低(t/P=31.808/<0.001、36.440/<0.001),健康对照组及非AIS组比较差异无统计学意义(t/P=2.559/0.152、3.113/0.067);重症亚组、中症亚组及轻症亚组AIS患者血清骨钙素及SIRT6依次降低(F/P=15.234/<0.001、30.388/<0.001)。与预后良好亚组比较,预后不良亚组AIS患者梗死面积大、NIHSS评分较高,血清骨钙素及SIRT6较低(χ^(2)/t=8.511、22.826、3.592、3.729,P均<0.001);梗死面积大、NIHSS评分高是影响AIS患者不良预后的独立危险因素[OR(95%CI)=1.397(1.141~1.709)、1.363(1.076~1.728)],血清骨钙素及SIRT6高是影响AIS患者不良预后的独立保护因素[OR(95%CI)=0.770(0.610~0.973)、0.709(0.549~0.915)]。血清骨钙素、SIRT6及两项联合对AIS患者不良预后预测的AUC分别为0.782、0.796、0.886,两项联合的AUC大于各自单独预测效能(Z=4.526、4.209,P均<0.001)。结论AIS患者血清骨钙素、SIRT6降低,两者与AIS病情严重程度有关,可作为评估AIS患者预后的血清生物标志物。 展开更多
关键词 急性缺血性脑卒中 骨钙素 去乙酰化酶6 预后
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昆明地区6462例成年人群骨代谢生化指标状况及影响因素分析
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作者 邵文萍 赵滢 +1 位作者 李昕 唐睿珠 《昆明医科大学学报》 CAS 2024年第11期130-136,共7页
目的探讨受试人群骨代谢生化指标与性别、年龄、季节的关系及骨代谢生化指标间影响关系。方法回顾性分析2021年1月至2024年4月期间昆明医科大学第一附属医院体检中心6462例健康体检结果资料。收集所有受试对象的性别、年龄、25-羟基维生... 目的探讨受试人群骨代谢生化指标与性别、年龄、季节的关系及骨代谢生化指标间影响关系。方法回顾性分析2021年1月至2024年4月期间昆明医科大学第一附属医院体检中心6462例健康体检结果资料。收集所有受试对象的性别、年龄、25-羟基维生素D(25-OH-VD)、降钙素(CT)、N-端骨钙素(N-MID)、β-胶原降解产物(Beta-CrossLaps,β-CTX)体检资料指标。根据骨代谢生化指标参考值范围、性别、年龄、季节进行分组,应用SPSS29.0软件对数据进行总体水平分布统计,比较组间骨代谢生化指标水平的差异性,分析骨代谢生化指标水平相关性,探讨骨代谢生化指标水平影响因素。结果生化指标水平总体中位数25-OHVD 61.93(49.17,77.12)nmol/L、CT1.43(.53,3.01)pg/mL、N-MID16.0(12.8,20.2)ng/mL、β-CTX397.0(295.0,527.0)pg/mL。不同性别、年龄和季节的血清骨代谢生化指标水平差异有统计学意义(P<0.05)。N-MID与β-CTX呈显著正相关(rs=0.748)。性别、年龄和季节对25-OH-VD和N-MID的影响差异有统计学意义(P<0.05)。结论受试人群25-OH-VD水平总体中位数低于正常参考值范围,维生素D水平普遍不足,在低龄受试人群中更普遍,提示补充维生素D可能是重要的公共卫生问题。CT、N-MID和β-CTX水平总体中位数处于正常参考值范围,表明在这些指标上受试群体健康状况较好。性别和年龄是25-OH-VD、CT和N-MID水平的显著影响因素;季节是25-OH-VD、N-MID和β-CTX水平的显著影响因素。N-MID与β-CTX可能存在密切的生物学联系。 展开更多
关键词 骨代谢生化指标 25-羟基维生素D 降钙素 N-端骨钙素 β-胶原降解产物
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血清BGP、miR-17-92、sST2与CHD患者PCI术后预后的关系
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作者 万又平 张国平 +2 位作者 李叔宝 郭伟崇 马金霞 《海南医学》 CAS 2024年第19期2817-2821,共5页
目的探讨骨钙素(BGP)、微小RNA-17-92a(miR-17-92)可溶性生长刺激表达基因2蛋白(sST2)与冠心病(CHD)患者经皮冠状动脉介入(PCI)术后预后的关系。方法选取2020年6月至2022年6月南阳市第一人民医院收治并行PCI术的168例CHD患者作为研究组... 目的探讨骨钙素(BGP)、微小RNA-17-92a(miR-17-92)可溶性生长刺激表达基因2蛋白(sST2)与冠心病(CHD)患者经皮冠状动脉介入(PCI)术后预后的关系。方法选取2020年6月至2022年6月南阳市第一人民医院收治并行PCI术的168例CHD患者作为研究组,另选取同期53例未行PCI的CHD患者作为对照组。比较两组患者的血清BGP、miR-17-92、sST2水平,研究组患者随访12个月,失访6例,依据预后情况分为预后不良组126例和预后良好组36例,采用多因素Logistic回归分析影响CHD患者PCI术后预后的危险因素;绘制受试者工作特征(ROC)曲线分析血清BGP、miR-17-92、s ST2对预后不良的预测效能。结果两组患者的血清BGP比较差异无统计学意义(P>0.05),但研究组患者的血清miR-17-92、sST2分别为1.55±0.29、(53.48±10.84)ng/mL,明显高于对照组的1.41±0.30、(20.64±5.97)ng/mL,差异均有统计学意义(P<0.05);预后不良组患者的miR-17-92、s ST2分别为1.59±0.29、(58.33±8.43)ng/mL,明显高于预后良好组的1.45±0.28、(52.15±6.86)ng/mL,而血清BGP水平为(23.06±3.84)ng/mL,明显低于预后良好组的(27.19±4.31)ng/mL,差异均有统计学意义(P<0.05);多因素Logistic回归分析结果显示,miR-17-92、sST2是CHD患者PCI术后预后的独立危险因素,而血清BGP是保护因素(P<0.05);ROC曲线分析结果显示,血清BGP、miR-17-92、s ST2单独及联合检测预测CHD患者PCI术后预后的曲线下面积(AUC)分别为0.783、0.628、0.723、0.856,联合预测的AUC明显高于单独预测(Z=2.897、4.627、1.984,P<0.05)。结论血清miR-17-92、sST2均是CHD患者PCI术后预后的危险因素,BGP是保护因素,监测其变化对CHD患者PCI术后预后有一定预测价值。 展开更多
关键词 冠心病 经皮冠状动脉介入术 微小RNA-17-92 骨钙素 可溶性生长刺激表达基因2蛋白 预后
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2型糖尿病病人外周血羧化不全骨钙素水平与合并非酒精性脂肪性肝病的关系分析
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作者 孟令华 位庚 +2 位作者 常湛 赵永晓 刘红利 《安徽医药》 CAS 2024年第10期2026-2030,共5页
目的检测2型糖尿病(T2DM)病人羧化不全骨钙素(ucOC)浓度,并分析其与合并非酒精性脂肪性肝病(NAFLD)的关系。方法选取石家庄市第二医院2020年6月至2022年6月收治的234例T2DM病人设为疾病组,根据病人是否合并NAFLD分为NAFLD组(107例)和非N... 目的检测2型糖尿病(T2DM)病人羧化不全骨钙素(ucOC)浓度,并分析其与合并非酒精性脂肪性肝病(NAFLD)的关系。方法选取石家庄市第二医院2020年6月至2022年6月收治的234例T2DM病人设为疾病组,根据病人是否合并NAFLD分为NAFLD组(107例)和非NAFLD组(127例),同期选取231例健康体检者为对照组,采用酶联免疫吸附测定检测受试者外周血ucOC浓度。比较NAFLD组和非NAFLD组病人的临床资料,采用多因素logistic回归分析法分析T2DM病人合并NAFLD的影响因素,并绘制受试者操作特征曲线(ROC曲线)评价外周血ucOC浓度对T2DM合并NAFLD的诊断价值。结果疾病组外周血ucOC浓度[(1.10±0.42)μg/L]低于对照组[(1.53±0.68)μg/L](P<0.05);NAFLD组ucOC[(0.98±0.14)μg/L]低于非NAFLD组[(1.20±0.21)μg/L](P<0.05)。NAFLD组身体质量指数(BMI)、腰臀比以及空腹胰岛素(FINS)、糖化血红蛋白(HbA1c)、舒张压、总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白(LDL-C)、尿酸、胰岛素抵抗指数(HOMA-IR)均高于非NAFLD组(P<0.05),高密度脂蛋白(HDL-C)低于非NAFLD组(P<0.05)。多因素结果显示,HbA1c、TG、尿酸及HOMA-IR升高均是T2DM病人合并NAFLD的危险因素(P<0.05),外周血ucOC浓度升高是其保护因素(P<0.05)。外周血ucOC浓度诊断T2DM病人合并NAFLD的最佳截断值、灵敏度、特异度、曲线下面积分别为1.05μg/L、83.18%、80.31%、0.84。结论T2DM病人外周血ucOC浓度降低,且合并NAFLD病人外周血ucOC浓度低于未合并病人。ucOC浓度与HbA1c、TG、尿酸、HOMA-IR均是T2DM病人合并NAFLD的影响因素,监测T2DM病人ucOC浓度变化有助于临床诊断NAFLD的发生。 展开更多
关键词 糖尿病 2型 非酒精性脂肪性肝病 羧化不全骨钙素 影响因素 受试者操作特征曲线
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基于PI3K/Akt/mTOR信号通路、性激素、骨代谢探究知柏地黄丸联合治疗女性特发性性早熟的效果及机制
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作者 陆影 龙海旭 《临床误诊误治》 CAS 2024年第6期82-88,共7页
目的探讨联合知柏地黄丸对女性特发性性早熟(CPP)患儿性激素、骨代谢及PI3K/Akt/mTOR信号通路的影响。方法选取2022年1月—2022年12月收治的60例CPP患儿,随机数字表法分为西药组、联合组2组,均30例。西药组给予注射用曲普瑞林治疗,联合... 目的探讨联合知柏地黄丸对女性特发性性早熟(CPP)患儿性激素、骨代谢及PI3K/Akt/mTOR信号通路的影响。方法选取2022年1月—2022年12月收治的60例CPP患儿,随机数字表法分为西药组、联合组2组,均30例。西药组给予注射用曲普瑞林治疗,联合组在西药组基础上联合知柏地黄丸治疗。比较2组临床疗效、安全性,以及治疗前后子宫体积、卵巢体积、卵泡直径、性激素水平[促黄体生成素(LH)、LH/促卵泡激素(FSH)、雌二醇(E_(2))]、骨代谢指标[骨钙素、骨碱性磷酸酶(BALP)、Ⅰ型前胶原氨基端肽(PⅠNP)、骨密度、β-胶原降解产物(β-CTX)]、PI3K/Akt/mTOR信号通路相关蛋白表达。结果联合组总有效率96.67%(29/30)高于西药组70.00%(21/30)(P<0.05)。联合组治疗后子宫体积、卵巢体积、卵泡直径均低于西药组(P<0.05);联合组治疗后血清LH、LH/FSH、E_(2)、骨钙素、BALP、PⅠNP水平低于西药组,骨密度高于西药组(P<0.05)。2组治疗后血清β-CTX水平比较无显著差异(P>0.05)。联合组治疗后PI3K、Akt、mTOR蛋白表达水平低于西药组(P<0.05)。联合组不良反应总发生率与西药组比较无显著差异(P>0.05)。结论注射用曲普瑞林联合知柏地黄丸能降低CPP患儿性激素水平,改善骨代谢相关因子的表达,抑制PI3K/Akt/mTOR信号通路活性,从而改善患儿症状。研究结果表明该方案临床疗效确切,且具有一定安全性。 展开更多
关键词 特发性性早熟 知柏地黄丸 曲普瑞林 PI3K/Akt/mTOR信号通路 雌二醇 黄体生成激素 骨钙素 骨密度
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特发性矮小症儿童血清IGFBP-3、25(OH)D、Nesfatin-1和骨钙素水平及其临床意义 被引量:1
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作者 叶丽 陈彬 王玲 《中南医学科学杂志》 CAS 2024年第1期106-109,共4页
目的探讨特发性矮小症(ISS)儿童血清胰岛素生长因子结合蛋白-3(IGFBP-3)、25羟维生素D[25(OH)D]、摄食抑制因子-1(Nesfatin-1)、骨钙素水平及其临床意义。方法选取矮小症儿童286例为观察组,其中ISS组152例,生长激素缺乏(GHD)组134例;同... 目的探讨特发性矮小症(ISS)儿童血清胰岛素生长因子结合蛋白-3(IGFBP-3)、25羟维生素D[25(OH)D]、摄食抑制因子-1(Nesfatin-1)、骨钙素水平及其临床意义。方法选取矮小症儿童286例为观察组,其中ISS组152例,生长激素缺乏(GHD)组134例;同时选取健康儿童100例为对照组。比较各组血清IGFBP-3、25(OH)D、Nesfatin-1、骨钙素水平差异,及其与体格发育等临床特征的关系。结果观察组儿童身高、骨龄指数、血清IGFBP-3、25(OH)D、骨钙素水平低于对照组,而Nesfatin-1高于对照组(P<0.05)。ISS组儿童身高、骨龄指数、血清IGFBP-3、25(OH)D、骨钙素水平高于GHD组,而Nesfatin-1低于GHD组(P<0.05)。血清IGFBP-3、25(OH)D和骨钙素水平身高≥125 cm儿童高于<125 cm儿童、骨龄指数≥0.90儿童高于<0.90儿童;而Nesfatin-1趋势相反(P<0.05)。ISS儿童血清IGFBP-3、25(OH)D、骨钙素与儿童身高、骨龄指数呈正相关(P<0.05),Nesfatin-1与儿童身高、骨龄指数呈负相关(P<0.05)。结论与健康儿童相比,ISS儿童血清IGFBP-3、25(OH)D、骨钙素水平较低,Nesfatin-1水平较高,可能与儿童体格发育存在相关性。 展开更多
关键词 特发性矮小症 IGFBP-3 25(OH)D NESFATIN-1 骨钙素
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血清BALP、BGP、β-CTx联合FRAX预测绝经后骨质疏松症患者髋部骨折风险的价值 被引量:1
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作者 石晓琦 王东岩 《检验医学与临床》 CAS 2024年第10期1478-1483,共6页
目的探讨血清骨碱性磷酸酶(BALP)、骨钙素(BGP)、β-Ⅰ型胶原羧基端肽(β-CTx)联合骨折风险评估工具(FRAX)预测绝经后骨质疏松症髋部骨折风险的价值。方法选取2020年1月至2022年12月该院收治的绝经后骨质疏松症患者132例纳入骨质疏松组... 目的探讨血清骨碱性磷酸酶(BALP)、骨钙素(BGP)、β-Ⅰ型胶原羧基端肽(β-CTx)联合骨折风险评估工具(FRAX)预测绝经后骨质疏松症髋部骨折风险的价值。方法选取2020年1月至2022年12月该院收治的绝经后骨质疏松症患者132例纳入骨质疏松组,另选择同期在该院诊断为骨量下降的123例和骨量正常的120例绝经后女性分别纳入骨量下降组和对照组。比较各组血清BALP、BGP、β-CTx水平及FRAX检测的髋部骨折风险。对绝经后骨质疏松症患者进行4~36个月的随访,根据骨质疏松症患者髋部是否骨折将其分为骨折组和未骨折组。采用多因素Logistic回归分析绝经后骨质疏松症患者髋部骨折的影响因素,绘制受试者工作特征(ROC)曲线分析血清BALP、BGP、β-CTx与FRAX单独及联合对绝经后骨质疏松症患者髋部骨折的预测价值。结果骨质疏松组血清BALP、BGP、β-CTx水平和FRAX检测的髋部骨折风险均高于骨量下降组和对照组,差异有统计学意义(P<0.05),骨量下降组血清BALP、BGP、β-CTx水平和FRAX检测的髋部骨折风险均高于对照组,差异有统计学意义(P<0.05)。132例绝经后骨质疏松症患者随访过程中,共有44例发生髋部骨折,发生率为33.33%。骨折组BALP、BGP、β-CTx水平和FRAX检测的髋部骨折风险均高于未骨折组,差异有统计学意义(P<0.05)。多因素Logistic回归分析结果显示,父母有髋部骨折史,有脆性骨折史、长期糖皮质激素治疗史、类风湿关节炎史,无规律补钙、缺乏阳光照射及血清BALP、BGP、β-CTx水平升高均是绝经后骨质疏松症患者髋部骨折的危险因素(P<0.05),骨密度(BMD)升高、体育锻炼则是绝经后骨质疏松症患者髋部骨折的保护因素(P<0.05)。ROC曲线分析结果显示,血清BALP、BGP、β-CTx联合FRAX预测绝经后骨质疏松症患者髋部骨折的灵敏度、曲线下面积(AUC)分别为95.45%、0.954,均高于单独预测(P<0.05)。结论血清BALP、BGP、β-CTx及FRAX均对绝经后骨质疏松症患者髋部骨折发生风险具有一定的预测价值,且4项指标或方法联合检测的预测价值更高。 展开更多
关键词 骨碱性磷酸酶 β-Ⅰ型胶原羧基端肽 骨钙素 骨折风险评估工具 绝经 骨质疏松症 髋部骨折
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骨关节炎中细胞因子、骨密度及骨标志物致病因素的研究进展
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作者 邢惠凯 李芳 +2 位作者 薛丽巾 刘玉芳 郑丽 《中国医药科学》 2024年第22期34-38,共5页
骨关节炎(OA)是关节的一种慢性退行性疾病,涉及关节软骨及其周围组织,其主要临床表现为关节肿痛、僵硬和活动受限,通常进展缓慢,但最终可导致关节功能障碍并伴有残疾,严重影响患者的生活质量。目前针对OA的治疗手段主要为缓解疼痛,不能... 骨关节炎(OA)是关节的一种慢性退行性疾病,涉及关节软骨及其周围组织,其主要临床表现为关节肿痛、僵硬和活动受限,通常进展缓慢,但最终可导致关节功能障碍并伴有残疾,严重影响患者的生活质量。目前针对OA的治疗手段主要为缓解疼痛,不能完全阻止关节组织的损伤或OA的进展。性别、年龄、体重指数、细胞因子、骨密度及骨标志物都参与了OA的发病,且多种致病因素间相互影响,共同导致疾病的发展。近年来,关于病因和发病机制的探索成为研究OA的主要方向。因此,本文将对细胞因子、骨密度及骨标志物在OA发病机制方面的研究进展做一综述,希望为OA的早期诊断及治疗提供新的思路。 展开更多
关键词 骨关节炎 细胞因子 骨密度 25羟维生素D 骨钙素 降钙素
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超重老年男性骨钙素与肌量的相关性研究
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作者 王青 邓慧 《实用老年医学》 CAS 2024年第5期482-485,共4页
目的观察超重老年男性病人中,骨钙素与骨骼肌质量的相关性。方法收集2020—2022年南京鼓楼医院老年科收治的167例超重老年男性病人为研究对象,根据骨钙素水平分为低骨钙素组和正常骨钙素组,分析骨钙素与超重老年男性腰部肌量的相关性,... 目的观察超重老年男性病人中,骨钙素与骨骼肌质量的相关性。方法收集2020—2022年南京鼓楼医院老年科收治的167例超重老年男性病人为研究对象,根据骨钙素水平分为低骨钙素组和正常骨钙素组,分析骨钙素与超重老年男性腰部肌量的相关性,并采用线性回归分析肌量的影响因素。结果2组间年龄、HbA1c、Ⅰ型胶原交联C末端肽(CTX)、Ⅰ型前胶原氨基端前肽(P1NP)、25-羟基维生素D、腰部肌量、髋部肌量差异均有统计学意义(P均<0.05)。骨钙素、CTX、P1NP均与髋部肌量及腰部肌量呈正相关(r=0.204~0.322,P均<0.05),年龄、BMI、腹围均与髋部肌量及腰部肌量呈负相关(r=-0.282~-0.187,P均<0.05)。多元线性回归分析显示,髋部肌量的独立影响因素为年龄、腹围(β=-0.081、-0.127,P均<0.05),腰部肌量的独立影响因素为年龄、腹围、骨钙素(β=-0.201、-0.136、0.187,P均<0.05)。结论超重老年男性病人腰部、髋部肌量与骨钙素呈正相关。骨钙素是腰部肌量的独立相关因素。 展开更多
关键词 老年男性 超重 肌量 骨钙素
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不同运动对男青年BMD、肌肉适能和肌骨共调节因子的影响
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作者 李庆 卜淑敏 +1 位作者 张丽 李龙菡 《中国骨质疏松杂志》 CAS CSCD 北大核心 2024年第8期1116-1121,1132,共7页
目的比较8周抗阻联合高强度间歇同期训练、单独高强度间歇训练和单独抗阻训练对男青年骨密度、肌肉适能以及血清肌骨共调节因子骨钙素和鸢尾素水平的影响。方法选取39名男青年,随机分为抗阻训练(RT)、高强度间歇训练(HIIT)和抗阻联合高... 目的比较8周抗阻联合高强度间歇同期训练、单独高强度间歇训练和单独抗阻训练对男青年骨密度、肌肉适能以及血清肌骨共调节因子骨钙素和鸢尾素水平的影响。方法选取39名男青年,随机分为抗阻训练(RT)、高强度间歇训练(HIIT)和抗阻联合高强度间歇同期训练(CT)三组后,分别进行为期8周的运动干预。分别在干预前和干预后48 h测试受试者的骨密度(bone mineral density,BMD)、体成分、最大摄氧量、肌肉力量、爆发力以及血清骨钙素和鸢尾素水平。结果三种运动干预均能显著提高男青年的瘦体重,但只有HIIT(P<0.01)和CT干预(P<0.05)能显著降低男青年的体脂率;三种运动干预均能显著提高男青年的股骨BMD,但只有HIIT(P<0.05)和RT(P<0.05)干预能显著提高男青年的腰椎BMD;RT和CT干预能显著提升男青年的卧推、硬拉、划船、深蹲的最大力量(P<0.01)和反向纵跳高度(P<0.01),而HIIT干预只能显著提升深蹲的最大力量(P<0.01);三种运动干预均能显著提高男青年的血清鸢尾素和骨钙素水平。结论三种运动干预均能增加男青年的瘦体重、股骨BMD以及血清肌骨调节因子鸢尾素和骨钙素水平,但CT对腰椎BMD的提升效果小于HIIT和RT,而RT和CT对肌肉力量和爆发力的提升效果大于HIIT。 展开更多
关键词 高强度间歇训练 抗阻训练 抗阻联合高强度间歇同期训练 骨密度 鸢尾素 骨钙素
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