Nucleotide-binding site leucine-rich repeat receptors (NBS-LRR/NLRs) are crucial intracellular immune proteins in plants. Previous article reported a novel NLR protein SUT1 (SUPPRESSORS OF TOPP4-1, 1), which is involv...Nucleotide-binding site leucine-rich repeat receptors (NBS-LRR/NLRs) are crucial intracellular immune proteins in plants. Previous article reported a novel NLR protein SUT1 (SUPPRESSORS OF TOPP4-1, 1), which is involved in autoimmunity initiated by type one protein phosphatase 4 mutation (topp4-1) in Arabidopsis, however, its role in planta is still unclear. This study employed Nicotiana benthamiana, a model platform, to conduct an overall structural and functional analysis of SUT1 protein. The transient expression results revealed that SUT1 is a typical CNL (CC-NBS-LRR) receptor, both fluorescence data and biochemical results showed the protein is mainly anchored on the plasma membrane due to its N-terminal acylation site. Further truncation experiments announced that its CC (coiled-coil) domain possessed cell-death-inducing activity. The outcomes of point mutations analysis revealed that not only the CC domain, but also the full-length SUT1 protein, whose function and subcellular localization are influenced by highly conserved hydrophobic residues. These research outcomes provided favorable clues for elucidating the activation mechanism of SUT1.展开更多
全球气候变化加重了沿海地区的气候暴露度,基于自然的解决方案(NbS)理念的活体海岸线(living shoreline)展示了更具韧性、更环境友好的海岸保护路径,但其相关基本理论和全生命周期管理框架尚未得到系统梳理。查阅国外相关部门项目公报及...全球气候变化加重了沿海地区的气候暴露度,基于自然的解决方案(NbS)理念的活体海岸线(living shoreline)展示了更具韧性、更环境友好的海岸保护路径,但其相关基本理论和全生命周期管理框架尚未得到系统梳理。查阅国外相关部门项目公报及Web of Science数据库,梳理近10年活体海岸线的相关研究与实践,从活体海岸线的分析规划、构造设计、施工建造、监测评估4个阶段进行总结分析,并结合实践应用和中国国情,客观看待目前活体海岸线的研究局限性;将其与传统硬质工程护岸进行辨证性的比较和思考,提出完善全生命周期管理的框架建议,旨在为我国的海岸保护与生境修复工作提供参考。展开更多
城市地下管廊内布设了大量的管线,如燃气管道、网络通讯线路、电力线路等,由于地下环境复杂多变,存在着气体泄漏、爆炸、火灾等安全风险。针对这些问题,提出一种基于窄带物联网技术(Narrow Band Internet of Things,NB-IoT)的地下管廊...城市地下管廊内布设了大量的管线,如燃气管道、网络通讯线路、电力线路等,由于地下环境复杂多变,存在着气体泄漏、爆炸、火灾等安全风险。针对这些问题,提出一种基于窄带物联网技术(Narrow Band Internet of Things,NB-IoT)的地下管廊环境监测系统。该系统采用先进的传感器技术、NB-IoT技术、软件技术,系统主要分为数据采集模块、物联网云平台、远程监测系统三部分。数据采集模块以STM32作为主控单元连接各个传感器,采集温度、湿度、水位、可燃气体等数据,经过处理后利用NB-IoT网络上传到物联网云平台,远程监测系统调用物联网云平台的数据接口进行远程显示与预警。实验结果表明,系统在降低系统总体功耗的同时,能够实时、稳定地进行地下管廊环境监测,提前预防可能存在的风险。展开更多
文摘Nucleotide-binding site leucine-rich repeat receptors (NBS-LRR/NLRs) are crucial intracellular immune proteins in plants. Previous article reported a novel NLR protein SUT1 (SUPPRESSORS OF TOPP4-1, 1), which is involved in autoimmunity initiated by type one protein phosphatase 4 mutation (topp4-1) in Arabidopsis, however, its role in planta is still unclear. This study employed Nicotiana benthamiana, a model platform, to conduct an overall structural and functional analysis of SUT1 protein. The transient expression results revealed that SUT1 is a typical CNL (CC-NBS-LRR) receptor, both fluorescence data and biochemical results showed the protein is mainly anchored on the plasma membrane due to its N-terminal acylation site. Further truncation experiments announced that its CC (coiled-coil) domain possessed cell-death-inducing activity. The outcomes of point mutations analysis revealed that not only the CC domain, but also the full-length SUT1 protein, whose function and subcellular localization are influenced by highly conserved hydrophobic residues. These research outcomes provided favorable clues for elucidating the activation mechanism of SUT1.
文摘全球气候变化加重了沿海地区的气候暴露度,基于自然的解决方案(NbS)理念的活体海岸线(living shoreline)展示了更具韧性、更环境友好的海岸保护路径,但其相关基本理论和全生命周期管理框架尚未得到系统梳理。查阅国外相关部门项目公报及Web of Science数据库,梳理近10年活体海岸线的相关研究与实践,从活体海岸线的分析规划、构造设计、施工建造、监测评估4个阶段进行总结分析,并结合实践应用和中国国情,客观看待目前活体海岸线的研究局限性;将其与传统硬质工程护岸进行辨证性的比较和思考,提出完善全生命周期管理的框架建议,旨在为我国的海岸保护与生境修复工作提供参考。
文摘城市地下管廊内布设了大量的管线,如燃气管道、网络通讯线路、电力线路等,由于地下环境复杂多变,存在着气体泄漏、爆炸、火灾等安全风险。针对这些问题,提出一种基于窄带物联网技术(Narrow Band Internet of Things,NB-IoT)的地下管廊环境监测系统。该系统采用先进的传感器技术、NB-IoT技术、软件技术,系统主要分为数据采集模块、物联网云平台、远程监测系统三部分。数据采集模块以STM32作为主控单元连接各个传感器,采集温度、湿度、水位、可燃气体等数据,经过处理后利用NB-IoT网络上传到物联网云平台,远程监测系统调用物联网云平台的数据接口进行远程显示与预警。实验结果表明,系统在降低系统总体功耗的同时,能够实时、稳定地进行地下管廊环境监测,提前预防可能存在的风险。
