BACKGROUND Mass spectrometry-based proteomics and glycomics reveal post-translational modifications providing significant biological insights beyond the scope of genomic sequencing.AIM To characterize the N-linked gly...BACKGROUND Mass spectrometry-based proteomics and glycomics reveal post-translational modifications providing significant biological insights beyond the scope of genomic sequencing.AIM To characterize the N-linked glycoproteomic profile in esophageal squamous cell carcinoma(ESCC)via two complementary approaches.METHODS Using tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins,we performed N-linked glycoproteomic profiling in ESCC tissues by two-dimensional gel electrophoresis(2-DE)-based and isobaric tags for relative and absolute quantification(iTRAQ)labeling-based mass spectrometry quantitation in parallel,followed by validation of candidate glycoprotein biomarkers by Western blot.RESULTS 2-DE-based and iTRAQ labeling-based quantitation identified 24 and 402 differentially expressed N-linked glycoproteins,respectively,with 15 in common,demonstrating the outperformance of iTRAQ labeling-based quantitation over 2-DE and complementarity of these two approaches.Proteomaps showed the distinct compositions of functional categories between proteins and glycoproteins with differential expression associated with ESCC.Western blot analysis validated the up-regulation of total procathepsin D and high-mannose procathepsin D,and the downregulation of total haptoglobin,high-mannose clusterin,and GlcNAc/sialic acid-containing fraction of 14-3-3ζ in ESCC tissues.The serum levels of glycosylated fractions of clusterin,prolinearginine-rich end leucine-rich repeat protein,and haptoglobin in patients with ESCC were remarkably higher than those in healthy controls.CONCLUSION Our study provides insights into the aberrant N-linked glycoproteome associated with ESCC,which will be a valuable resource for future investigations.展开更多
目的:观察葛根素能否在体外减少葡萄糖腹膜透析液中晚期糖基化终末产物(AGEs)的产生。方法:将20 g牛血清白蛋白充分溶解于1 000 m l 2.5%腹膜透析液中,根据葛根素终浓度分5组观察(葛根素终浓度A组为0 g.L-1,B组为0.01 g.L-1,C组为0.05 g...目的:观察葛根素能否在体外减少葡萄糖腹膜透析液中晚期糖基化终末产物(AGEs)的产生。方法:将20 g牛血清白蛋白充分溶解于1 000 m l 2.5%腹膜透析液中,根据葛根素终浓度分5组观察(葛根素终浓度A组为0 g.L-1,B组为0.01 g.L-1,C组为0.05 g.L-1,D组为0.1 g.L-1,E组为0.2 g.L-1)。过滤除菌后37℃保存,分别于第1天、7天、1个月、2个月以及3个月时取样本1 m l进行AGEs测定。结果:腹膜透析液中添加牛血清白蛋白后,随着时间的延长,透析液中AGEs的浓度逐渐升高。加入不同浓度葛根素的试验组,其AGEs的浓度均较同期未加入葛根素的试验组明显降低,并且随着葛根素浓度增加,AGEs的浓度渐降。结论:葛根素对体外腹膜透析液中AGEs形成有明显的抑制作用,且它的这种抑制作用呈现剂量依赖性。展开更多
The definition of impaired glucose tolerance (IGT) was first introduced by the National Diabetes Data Group at 1979. Recently the morbidity rate of IGT is soaring. About 10%-15% of patients with IGT will progress to t...The definition of impaired glucose tolerance (IGT) was first introduced by the National Diabetes Data Group at 1979. Recently the morbidity rate of IGT is soaring. About 10%-15% of patients with IGT will progress to type 2 diabetes mellitus (T2DM) which is approximately more than 100 times comparing with normal glucose tolerance people. Angiopathy can be detected in 40% patients with newly diagnosed IGT. Advanced glycation end products (AGEs) are closely related to angiopathy. Meanwhile there is some connection between IGT and AGEs. In this review, the relationship between IGT, agiopathy and AGEs is discussed.展开更多
A novel one-step method for synthesis of paeonol,which was prepared from m-methoxyl phenol and acetic acid under catalyst of ZnCl2,with yield 68% was reported in this article.In addition,in order to improve water-solu...A novel one-step method for synthesis of paeonol,which was prepared from m-methoxyl phenol and acetic acid under catalyst of ZnCl2,with yield 68% was reported in this article.In addition,in order to improve water-solubility of paeonol,the glycosylation reactions of paeonol and 2,4-dihydroxyphenyl ethanone,which was metabolized intermediate of paeonol in organism,were carried out to modify their structures.Four glycosylation derivatives of paeonol and 2,4-dihydroxyphenyl ethanone reacted with α-bromide tetra-O-acetly-glucopyranose and α-bromide tetra-O-acetly-galactopyranose were synthesized,with yield 30-73%.Paeonol and its glycosylation derivatives were characterized by1HNMR.展开更多
目的对糖化血红蛋白分析仪(HPLC-723 G7)进行方法性能监控、应用能力分析,旨在充分了解其性能以保证糖化血红蛋白(HbA1c)检测结果在糖尿病血糖控制、并发症防治中的积极作用。方法参照美国病理医学院(Col-lege of American Pathologists...目的对糖化血红蛋白分析仪(HPLC-723 G7)进行方法性能监控、应用能力分析,旨在充分了解其性能以保证糖化血红蛋白(HbA1c)检测结果在糖尿病血糖控制、并发症防治中的积极作用。方法参照美国病理医学院(Col-lege of American Pathologists,CAP)的能力对比检验要求和卫生部《临床实验室管理办法》,进行精密度、稀释线性分析、标本稀释后的稳定性、不同仪器间比对、干扰物质的影响、能力比对、质量控制过程能力指数计算。结果显示干扰试验不受各种共存物影响;标本稀释后稳定性监测良好,稀释有效性试验良好;不同仪器间比对:与BIO-RADVariantⅡ型分析仪进行相关性比较,Y=0.986X+0.072,r=0.9980;CAP的PT回报结果:与靶值很接近,结果L:5.3%、M:8.7%、H:11.4%;CAP结果L:5.23%、M∶8.61%、H:11.27%;质量控制过程能力指数(ProcessCa-pabiltyIndex)计算:CP=Tea/3S,CP=3.57,CP>1.33。结论糖化血红蛋白分析仪(HPLC-723G7)的应用能力满足临床质量要求。展开更多
基金Supported by National Natural Science Foundation of China,No.81072039 and No.81872037.
文摘BACKGROUND Mass spectrometry-based proteomics and glycomics reveal post-translational modifications providing significant biological insights beyond the scope of genomic sequencing.AIM To characterize the N-linked glycoproteomic profile in esophageal squamous cell carcinoma(ESCC)via two complementary approaches.METHODS Using tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins,we performed N-linked glycoproteomic profiling in ESCC tissues by two-dimensional gel electrophoresis(2-DE)-based and isobaric tags for relative and absolute quantification(iTRAQ)labeling-based mass spectrometry quantitation in parallel,followed by validation of candidate glycoprotein biomarkers by Western blot.RESULTS 2-DE-based and iTRAQ labeling-based quantitation identified 24 and 402 differentially expressed N-linked glycoproteins,respectively,with 15 in common,demonstrating the outperformance of iTRAQ labeling-based quantitation over 2-DE and complementarity of these two approaches.Proteomaps showed the distinct compositions of functional categories between proteins and glycoproteins with differential expression associated with ESCC.Western blot analysis validated the up-regulation of total procathepsin D and high-mannose procathepsin D,and the downregulation of total haptoglobin,high-mannose clusterin,and GlcNAc/sialic acid-containing fraction of 14-3-3ζ in ESCC tissues.The serum levels of glycosylated fractions of clusterin,prolinearginine-rich end leucine-rich repeat protein,and haptoglobin in patients with ESCC were remarkably higher than those in healthy controls.CONCLUSION Our study provides insights into the aberrant N-linked glycoproteome associated with ESCC,which will be a valuable resource for future investigations.
文摘目的:观察葛根素能否在体外减少葡萄糖腹膜透析液中晚期糖基化终末产物(AGEs)的产生。方法:将20 g牛血清白蛋白充分溶解于1 000 m l 2.5%腹膜透析液中,根据葛根素终浓度分5组观察(葛根素终浓度A组为0 g.L-1,B组为0.01 g.L-1,C组为0.05 g.L-1,D组为0.1 g.L-1,E组为0.2 g.L-1)。过滤除菌后37℃保存,分别于第1天、7天、1个月、2个月以及3个月时取样本1 m l进行AGEs测定。结果:腹膜透析液中添加牛血清白蛋白后,随着时间的延长,透析液中AGEs的浓度逐渐升高。加入不同浓度葛根素的试验组,其AGEs的浓度均较同期未加入葛根素的试验组明显降低,并且随着葛根素浓度增加,AGEs的浓度渐降。结论:葛根素对体外腹膜透析液中AGEs形成有明显的抑制作用,且它的这种抑制作用呈现剂量依赖性。
文摘The definition of impaired glucose tolerance (IGT) was first introduced by the National Diabetes Data Group at 1979. Recently the morbidity rate of IGT is soaring. About 10%-15% of patients with IGT will progress to type 2 diabetes mellitus (T2DM) which is approximately more than 100 times comparing with normal glucose tolerance people. Angiopathy can be detected in 40% patients with newly diagnosed IGT. Advanced glycation end products (AGEs) are closely related to angiopathy. Meanwhile there is some connection between IGT and AGEs. In this review, the relationship between IGT, agiopathy and AGEs is discussed.
文摘A novel one-step method for synthesis of paeonol,which was prepared from m-methoxyl phenol and acetic acid under catalyst of ZnCl2,with yield 68% was reported in this article.In addition,in order to improve water-solubility of paeonol,the glycosylation reactions of paeonol and 2,4-dihydroxyphenyl ethanone,which was metabolized intermediate of paeonol in organism,were carried out to modify their structures.Four glycosylation derivatives of paeonol and 2,4-dihydroxyphenyl ethanone reacted with α-bromide tetra-O-acetly-glucopyranose and α-bromide tetra-O-acetly-galactopyranose were synthesized,with yield 30-73%.Paeonol and its glycosylation derivatives were characterized by1HNMR.
文摘目的对糖化血红蛋白分析仪(HPLC-723 G7)进行方法性能监控、应用能力分析,旨在充分了解其性能以保证糖化血红蛋白(HbA1c)检测结果在糖尿病血糖控制、并发症防治中的积极作用。方法参照美国病理医学院(Col-lege of American Pathologists,CAP)的能力对比检验要求和卫生部《临床实验室管理办法》,进行精密度、稀释线性分析、标本稀释后的稳定性、不同仪器间比对、干扰物质的影响、能力比对、质量控制过程能力指数计算。结果显示干扰试验不受各种共存物影响;标本稀释后稳定性监测良好,稀释有效性试验良好;不同仪器间比对:与BIO-RADVariantⅡ型分析仪进行相关性比较,Y=0.986X+0.072,r=0.9980;CAP的PT回报结果:与靶值很接近,结果L:5.3%、M:8.7%、H:11.4%;CAP结果L:5.23%、M∶8.61%、H:11.27%;质量控制过程能力指数(ProcessCa-pabiltyIndex)计算:CP=Tea/3S,CP=3.57,CP>1.33。结论糖化血红蛋白分析仪(HPLC-723G7)的应用能力满足临床质量要求。
