N-methyl-D-aspartate receptor hypofunction is the basis of pathophysiology in schizophrenia. Blocking the N-methyl-D-aspartate receptor impairs learning and memory abilities and induces pathological changes in the bra...N-methyl-D-aspartate receptor hypofunction is the basis of pathophysiology in schizophrenia. Blocking the N-methyl-D-aspartate receptor impairs learning and memory abilities and induces pathological changes in the brain. Previous studies have paid little attention to the role of the N-methyl-D-aspartate receptor subunit 1 (NR1) in neurogenesis in the hippocampus of schizophrenia. A mouse model of schizophrenia was established by intraperitoneal injection of 0.6 mg/kg MK-801, once a day, for 14 days. In N-methyl-D-aspartate-treated mice, N-methyl-D-aspartate was administered by intracerebroventricular injection in schizophrenia mice on day 15. The number of NR1-, Ki67- or BrdU-immunoreactive cells in the dentate gyrus was measured by immunofluorescence staining. Our data showed the number of NR1-immunoreactive cells increased along with the decreasing numbers of BrdU- and Ki67-immunoreactive cells in the schizophrenia groups compared with the control group. N-methyl-D-aspartate could reverse the above changes. These results indicated that NR1 can regulate neurogenesis in the hippocampal dentate gyrus of schizophrenia mice, supporting NR1 as a promising therapeutic target in the treatment of schizophrenia. This study was approved by the Experimental Animal Ethics Committee of the Ningxia Medical University, China (approval No. 2014-014) on March 6, 2014.展开更多
AIM: To investigate whether in the prefrontal cortical(PFC) pyramidal eells, focal glutamate application tothe apical dendrite induces bursting and whether theeffect of glutamate involves activation of both NMDAand no...AIM: To investigate whether in the prefrontal cortical(PFC) pyramidal eells, focal glutamate application tothe apical dendrite induces bursting and whether theeffect of glutamate involves activation of both NMDAand non-NMDA receptors. METHODS: Pyramidalcells in layers Ⅴ and Ⅵ of the PFC were visualized inrat brain slices using infrared videomicroscopy andrecorded with whole-cell electrodes. Glutamate and itsagonists were focally applied to the apical dendrite andthe soma using microiontophoresis. RESULTS:Dendritic glutamate application (0--20 nA, 10 mmol/L) induced repetitive bursts in most cells tested (12/展开更多
基金supported by the National Natural Science Foundation of China,No.81160169(to JL),81460214(to JL),31660270(to JD),31460255(to JD)the Natural Science Foundation of Ningxia Hui Autonomous Region of China,No.2018AAC02005(to JL)
文摘N-methyl-D-aspartate receptor hypofunction is the basis of pathophysiology in schizophrenia. Blocking the N-methyl-D-aspartate receptor impairs learning and memory abilities and induces pathological changes in the brain. Previous studies have paid little attention to the role of the N-methyl-D-aspartate receptor subunit 1 (NR1) in neurogenesis in the hippocampus of schizophrenia. A mouse model of schizophrenia was established by intraperitoneal injection of 0.6 mg/kg MK-801, once a day, for 14 days. In N-methyl-D-aspartate-treated mice, N-methyl-D-aspartate was administered by intracerebroventricular injection in schizophrenia mice on day 15. The number of NR1-, Ki67- or BrdU-immunoreactive cells in the dentate gyrus was measured by immunofluorescence staining. Our data showed the number of NR1-immunoreactive cells increased along with the decreasing numbers of BrdU- and Ki67-immunoreactive cells in the schizophrenia groups compared with the control group. N-methyl-D-aspartate could reverse the above changes. These results indicated that NR1 can regulate neurogenesis in the hippocampal dentate gyrus of schizophrenia mice, supporting NR1 as a promising therapeutic target in the treatment of schizophrenia. This study was approved by the Experimental Animal Ethics Committee of the Ningxia Medical University, China (approval No. 2014-014) on March 6, 2014.
基金Scottish Rite Benevolent Foundation's Schizophrenia Research Program (Shi WX)a NASARD Young Investigator Award (Shi WX)an USPHS grant MH52686(shi WX)
文摘AIM: To investigate whether in the prefrontal cortical(PFC) pyramidal eells, focal glutamate application tothe apical dendrite induces bursting and whether theeffect of glutamate involves activation of both NMDAand non-NMDA receptors. METHODS: Pyramidalcells in layers Ⅴ and Ⅵ of the PFC were visualized inrat brain slices using infrared videomicroscopy andrecorded with whole-cell electrodes. Glutamate and itsagonists were focally applied to the apical dendrite andthe soma using microiontophoresis. RESULTS:Dendritic glutamate application (0--20 nA, 10 mmol/L) induced repetitive bursts in most cells tested (12/
