[Objective] The research aimed to study the polymorphism of NCOA1 gene in pigs by PCR-RFLP.[Method] Using 81 Landrace pigs from Liuhe pig breeding farm as research objects,the genome DNA was extracted from ear tissue ...[Objective] The research aimed to study the polymorphism of NCOA1 gene in pigs by PCR-RFLP.[Method] Using 81 Landrace pigs from Liuhe pig breeding farm as research objects,the genome DNA was extracted from ear tissue of pigs by conventional Phenol-chloroform extraction method.The genomic DNA was used as template to make PCR amplification and a 440 bp target fragment was obtained.PCR amplification products were digested by restriction nuclease Rsa I.The agarose gel electrophoresis detection was made.And the gene frequency and the genotype frequency were calculated.[Result] The agarose gel electrophoresis detection showed that the enzyme digestion products had three genotypes of AA （440 bp）,AB （440 bp,282 bp,158 bp） and BB （282 bp,158 bp）：The genotype frequencies of AA,AB and BB were 0.54,0.43 and 0.03,respectively：The frequencies of allele A and B were 0.76 and 0.24,respectively.[Conclusion] In the studied pig herd,A is the dominant allele and the genotype frequency of AA is higher.展开更多
鸡核受体辅激活蛋白1基因(NCOA1)mRNA的表达对性腺的发育、成熟均起关键作用,并且与繁殖性状相关。为了建立荧光定量PCR技术检测鸡NCOA1表达量的方法,根据GenBank中NCOA1基因序列设计合成了引物,以β-action基因为内参基因,对荧光定量PC...鸡核受体辅激活蛋白1基因(NCOA1)mRNA的表达对性腺的发育、成熟均起关键作用,并且与繁殖性状相关。为了建立荧光定量PCR技术检测鸡NCOA1表达量的方法,根据GenBank中NCOA1基因序列设计合成了引物,以β-action基因为内参基因,对荧光定量PCR方法进行了评估。结果表明:构建的标准曲线线性关系良好,建立的NCOA1基因荧光定量PCR检测方法灵敏度高、特异性强,准确可靠。建立的SYBR Green I实时荧光定量PCR方法可以测定鸡NCOA1mRNA表达水平的相对含量。展开更多
基金Supported by Major Specialized Subject of Transgenic Organism New Variety Breeding(20082X08006-003)National Natural Science Foundation of China(30871778)Construction Engineering Special Fund for Mountain Tai Scholars of Shandong Province~~
文摘[Objective] The research aimed to study the polymorphism of NCOA1 gene in pigs by PCR-RFLP.[Method] Using 81 Landrace pigs from Liuhe pig breeding farm as research objects,the genome DNA was extracted from ear tissue of pigs by conventional Phenol-chloroform extraction method.The genomic DNA was used as template to make PCR amplification and a 440 bp target fragment was obtained.PCR amplification products were digested by restriction nuclease Rsa I.The agarose gel electrophoresis detection was made.And the gene frequency and the genotype frequency were calculated.[Result] The agarose gel electrophoresis detection showed that the enzyme digestion products had three genotypes of AA （440 bp）,AB （440 bp,282 bp,158 bp） and BB （282 bp,158 bp）：The genotype frequencies of AA,AB and BB were 0.54,0.43 and 0.03,respectively：The frequencies of allele A and B were 0.76 and 0.24,respectively.[Conclusion] In the studied pig herd,A is the dominant allele and the genotype frequency of AA is higher.
文摘鸡核受体辅激活蛋白1基因(NCOA1)mRNA的表达对性腺的发育、成熟均起关键作用,并且与繁殖性状相关。为了建立荧光定量PCR技术检测鸡NCOA1表达量的方法,根据GenBank中NCOA1基因序列设计合成了引物,以β-action基因为内参基因,对荧光定量PCR方法进行了评估。结果表明:构建的标准曲线线性关系良好,建立的NCOA1基因荧光定量PCR检测方法灵敏度高、特异性强,准确可靠。建立的SYBR Green I实时荧光定量PCR方法可以测定鸡NCOA1mRNA表达水平的相对含量。
