通过体外实验研究副干酪乳杆菌NCU622的耐酸、耐胆盐性能;采用体外细胞模型(人体结肠腺癌细胞系Caco-2细胞)测定副干酪乳杆菌NCU622的黏附性能,考察了菌体浓度、作用时间及生长阶段对其黏附性能的影响,并对该菌株进行细胞毒性检测。结...通过体外实验研究副干酪乳杆菌NCU622的耐酸、耐胆盐性能;采用体外细胞模型(人体结肠腺癌细胞系Caco-2细胞)测定副干酪乳杆菌NCU622的黏附性能,考察了菌体浓度、作用时间及生长阶段对其黏附性能的影响,并对该菌株进行细胞毒性检测。结果表明:副干酪乳杆菌NCU622在p H 2.5的MRS液体培养基中作用3 h,存活率为98.21%;在胆盐质量浓度为0.3、0.5 g/100 m L的MRS液体培养基中作用4 h,存活率分别为95.14%和85.07%。副干酪乳杆菌NCU622对Caco-2细胞的黏附能力较强,为(21.19±0.94)CFU/Caco-2细胞,且菌体浓度、作用时间及生长阶段对其黏附性能均有影响。副干酪乳杆菌NCU622与Caco-2单层细胞共培养24 h后,对其无裂解作用。这表明副干酪乳杆菌NCU622具有良好的耐酸耐胆盐能力及较强的黏附性能,且对Caco-2细胞无裂解作用,符合微生态制剂和乳酸菌发酵功能食品的菌种要求。展开更多
神经科重症监护室（neurology care unit,NCU）成立较晚,工作环境特殊,危重患者多,各种复杂的仪器操作和无陪护使NCU护士工作压力比临床普通科室护士大。压力对健康的影响是双向的,适当的压力有利于提高人体的适应能力,但持久而慢性的...神经科重症监护室（neurology care unit,NCU）成立较晚,工作环境特殊,危重患者多,各种复杂的仪器操作和无陪护使NCU护士工作压力比临床普通科室护士大。压力对健康的影响是双向的,适当的压力有利于提高人体的适应能力,但持久而慢性的、超过人们承受能力的压力,使人长期处于紧张状态,降低人的活力和发展的信心,从而导致身心疾病[1]。展开更多
The NCU (National Central University) Lu Lin Observatory (Fig. 1) is located at Mt. Front Lu Lin, 120°52′25″E and 23°28′07″N, a 2862 m peak in the Yu Shan National Park. The construction of Lu Lin Observ...The NCU (National Central University) Lu Lin Observatory (Fig. 1) is located at Mt. Front Lu Lin, 120°52′25″E and 23°28′07″N, a 2862 m peak in the Yu Shan National Park. The construction of Lu Lin Observatory was finished on January 14, 1999. The initial study of Lu Lin site was started since late 1989. Later on, a three year project was founded by the National Science Council (NSC) which supported the development of a modern seeing monitor for this site survey study from 1990 through 1993. The average seeing of Lu Lin site is about 1.39 arc second with average 200 clear nights annually. The sky background of this site is 20.72 mag/arcsec 2 in V band and 21.22 mag/arcsec 2 in B band. The Lu Lin observatory is developed for both research and education activity. A homemade 76 cm Super Light Telescope (SLT) and three TAOS’s 50 cm robotic telescopes will be the two major research facilities. The SLT is expected to be operated in the fall of 1999. We are proposing a pilot program to perform SLT observations of time varying astrophysical objects exploiting the unique geophysical location (e. g. time and longitudinal coverage) of Taiwan at the Western Pacific Rim in such topics of emerging interest. The TAOS’s telescopes will be installed in the late 1999 for the survey work of Kuiper Belt Objects. A wireless T1 Network system will also be build up for future remote observation.展开更多
文摘通过体外实验研究副干酪乳杆菌NCU622的耐酸、耐胆盐性能;采用体外细胞模型(人体结肠腺癌细胞系Caco-2细胞)测定副干酪乳杆菌NCU622的黏附性能,考察了菌体浓度、作用时间及生长阶段对其黏附性能的影响,并对该菌株进行细胞毒性检测。结果表明:副干酪乳杆菌NCU622在p H 2.5的MRS液体培养基中作用3 h,存活率为98.21%;在胆盐质量浓度为0.3、0.5 g/100 m L的MRS液体培养基中作用4 h,存活率分别为95.14%和85.07%。副干酪乳杆菌NCU622对Caco-2细胞的黏附能力较强,为(21.19±0.94)CFU/Caco-2细胞,且菌体浓度、作用时间及生长阶段对其黏附性能均有影响。副干酪乳杆菌NCU622与Caco-2单层细胞共培养24 h后,对其无裂解作用。这表明副干酪乳杆菌NCU622具有良好的耐酸耐胆盐能力及较强的黏附性能,且对Caco-2细胞无裂解作用,符合微生态制剂和乳酸菌发酵功能食品的菌种要求。
文摘神经科重症监护室（neurology care unit,NCU）成立较晚,工作环境特殊,危重患者多,各种复杂的仪器操作和无陪护使NCU护士工作压力比临床普通科室护士大。压力对健康的影响是双向的,适当的压力有利于提高人体的适应能力,但持久而慢性的、超过人们承受能力的压力,使人长期处于紧张状态,降低人的活力和发展的信心,从而导致身心疾病[1]。
文摘The NCU (National Central University) Lu Lin Observatory (Fig. 1) is located at Mt. Front Lu Lin, 120°52′25″E and 23°28′07″N, a 2862 m peak in the Yu Shan National Park. The construction of Lu Lin Observatory was finished on January 14, 1999. The initial study of Lu Lin site was started since late 1989. Later on, a three year project was founded by the National Science Council (NSC) which supported the development of a modern seeing monitor for this site survey study from 1990 through 1993. The average seeing of Lu Lin site is about 1.39 arc second with average 200 clear nights annually. The sky background of this site is 20.72 mag/arcsec 2 in V band and 21.22 mag/arcsec 2 in B band. The Lu Lin observatory is developed for both research and education activity. A homemade 76 cm Super Light Telescope (SLT) and three TAOS’s 50 cm robotic telescopes will be the two major research facilities. The SLT is expected to be operated in the fall of 1999. We are proposing a pilot program to perform SLT observations of time varying astrophysical objects exploiting the unique geophysical location (e. g. time and longitudinal coverage) of Taiwan at the Western Pacific Rim in such topics of emerging interest. The TAOS’s telescopes will be installed in the late 1999 for the survey work of Kuiper Belt Objects. A wireless T1 Network system will also be build up for future remote observation.
