In a preliminary investigation, Random Amplified Polymorphic DNA (RAPD) analysis and partial mitochondrial ND2 gene sequencing were conducted to study the genetic variation of the Indian mackerel, Rastrelliger kanagur...In a preliminary investigation, Random Amplified Polymorphic DNA (RAPD) analysis and partial mitochondrial ND2 gene sequencing were conducted to study the genetic variation of the Indian mackerel, Rastrelliger kanagurta along a 450 km stretch of its distribution on the west coast of Peninsular Malaysia. A total of 53 individuals from 6 populations were analyzed using 4 RAPD primers and a sub-sample of 15 individuals was chosen for sequencing of partial ND2 gene. Comparison between the 2 markers revealed genetic structuring in the RAPD results but genetic homogeneity for ND2 gene. Based on the former there may be at least 2 genetically differentiated groups of Rastrelliger kanagurta along this stretch.展开更多
[Objective] The study was to analyze the phylogenesis of Anas platyrhynchos. [Method] Complete sequence of mitochondrial ND2 gene of 4 Anas platyrhynchos was determined by direct DNA sequencing based on PCR products. ...[Objective] The study was to analyze the phylogenesis of Anas platyrhynchos. [Method] Complete sequence of mitochondrial ND2 gene of 4 Anas platyrhynchos was determined by direct DNA sequencing based on PCR products. Combined with ND2 gene sequences of the Anas Linnaeus accessed in GenBank, phylogenetic tree was constructed by Neighbor-joining and maximum parsimony methods. [Result] The ND2 gene sequences of 4 Anas platyrhynchos were identical(1 041 bp in length; the nucleotide contents of A, G, T, and C were 28.91%, 13.35%, 20.75% and 36.98% respectively; A+T content approximated to that of C+G). Sequences of ND2 gene of mallard were same as spotbill duck, and had high homology with others. The phylogenetic trees indicated mallard and spotbilled duck were close in genetic relationship, both shared a haplotype; then Philippine duck, green-winged teal and northern pintail fell into branch ''A". [Conclusion] The domestic duck may be domesticated from mallard and spotbilled duck.展开更多
Degenerate primers were designed according to the conserved sequences of NADH dehydrogenase subunit 2（ND2） gene in mitochondrial tRNAMet and tRNATrp gene respectively,and the complete sequence of the ND2 gene of Eri...Degenerate primers were designed according to the conserved sequences of NADH dehydrogenase subunit 2（ND2） gene in mitochondrial tRNAMet and tRNATrp gene respectively,and the complete sequence of the ND2 gene of Eriocheir japonica was determined.The results indicated that the length of ND2 gene of Eriocheir japonica was 1009 bp.The A,T,G,C contents of the gene were 28.54%（288 bp）,44.20%（446 bp）,8.23%（83 bp）,and 19.03%（192 bp） respectively;and the sequence was AT rich and biased.The start and stop codons were ATC and A respectively.The homology analysis of nucleotide sequence showed that the similarities in ND2 gene between Eriocheir japonica and other crustacean were comparatively low,which suggested that ND2 gene could be effective molecular marker for further study on phylogeny of Eriocheir species.展开更多
基金funded by the Ninth Malaysian Plan under the Department of Fisheries Malaysian.
文摘In a preliminary investigation, Random Amplified Polymorphic DNA (RAPD) analysis and partial mitochondrial ND2 gene sequencing were conducted to study the genetic variation of the Indian mackerel, Rastrelliger kanagurta along a 450 km stretch of its distribution on the west coast of Peninsular Malaysia. A total of 53 individuals from 6 populations were analyzed using 4 RAPD primers and a sub-sample of 15 individuals was chosen for sequencing of partial ND2 gene. Comparison between the 2 markers revealed genetic structuring in the RAPD results but genetic homogeneity for ND2 gene. Based on the former there may be at least 2 genetically differentiated groups of Rastrelliger kanagurta along this stretch.
基金Supported by National Key Technology R&D program(2006BAD06B06)National Infrastructure of Natural Resources for Science and Technology(2004DKA30460)~~
文摘[Objective] The study was to analyze the phylogenesis of Anas platyrhynchos. [Method] Complete sequence of mitochondrial ND2 gene of 4 Anas platyrhynchos was determined by direct DNA sequencing based on PCR products. Combined with ND2 gene sequences of the Anas Linnaeus accessed in GenBank, phylogenetic tree was constructed by Neighbor-joining and maximum parsimony methods. [Result] The ND2 gene sequences of 4 Anas platyrhynchos were identical(1 041 bp in length; the nucleotide contents of A, G, T, and C were 28.91%, 13.35%, 20.75% and 36.98% respectively; A+T content approximated to that of C+G). Sequences of ND2 gene of mallard were same as spotbill duck, and had high homology with others. The phylogenetic trees indicated mallard and spotbilled duck were close in genetic relationship, both shared a haplotype; then Philippine duck, green-winged teal and northern pintail fell into branch ''A". [Conclusion] The domestic duck may be domesticated from mallard and spotbilled duck.
文摘Degenerate primers were designed according to the conserved sequences of NADH dehydrogenase subunit 2（ND2） gene in mitochondrial tRNAMet and tRNATrp gene respectively,and the complete sequence of the ND2 gene of Eriocheir japonica was determined.The results indicated that the length of ND2 gene of Eriocheir japonica was 1009 bp.The A,T,G,C contents of the gene were 28.54%（288 bp）,44.20%（446 bp）,8.23%（83 bp）,and 19.03%（192 bp） respectively;and the sequence was AT rich and biased.The start and stop codons were ATC and A respectively.The homology analysis of nucleotide sequence showed that the similarities in ND2 gene between Eriocheir japonica and other crustacean were comparatively low,which suggested that ND2 gene could be effective molecular marker for further study on phylogeny of Eriocheir species.
