目的筛选人源膜表面黏附分子NECL1蛋白胞内区相互作用蛋白。方法构建含人NECL1蛋白胞内区氨基酸编码序列的诱饵质粒pGBKT7N-ECL1C,对人胎脑cDNA文库进行酵母双杂交筛选。用GST pu ll down实验进行体外蛋白相互作用的验证。结果酵母双杂...目的筛选人源膜表面黏附分子NECL1蛋白胞内区相互作用蛋白。方法构建含人NECL1蛋白胞内区氨基酸编码序列的诱饵质粒pGBKT7N-ECL1C,对人胎脑cDNA文库进行酵母双杂交筛选。用GST pu ll down实验进行体外蛋白相互作用的验证。结果酵母双杂交阳性克隆测序后显示共存在9段不同序列(存在重复克隆)。比对氨基酸序列得到5个可能相互作用蛋白。通过GST pu ll down实验验证了其中两个蛋白与NECL1胞内区的相互作用。结论应用酵母双杂交系统,获得了一些候选的NECL1胞内区相互作用蛋白。展开更多
Non-equal channel lateral extrusion（NECLE） is a new process that can be used to attain higher grain refinement in comparison with equal channel lateral extrusion（ECLE）. The die design for this process was numerica...Non-equal channel lateral extrusion（NECLE） is a new process that can be used to attain higher grain refinement in comparison with equal channel lateral extrusion（ECLE）. The die design for this process was numerically and experimentally studied. After finding a good correlation between the numerical and experimental results, more comprehensive FE analyses were carried out. Different die geometrical parameters were considered and their effects on the induced plastic strain, stress distribution, velocity field and forming load of the process were investigated. It was found that by this process with a suitable set of die geometrical parameters, higher induced effective strain and more homogeneous strain distribution could be achieved in comparison with ECLE operation.展开更多
A pathogenic-related gene nec1 was cloned in Streptomyces scabies CPS-1,potato scab strain.Analysis results showed that the length of open reading frame(ORF)for nec1 gene was 666 bp,and the GC content was 54.2%.Sequen...A pathogenic-related gene nec1 was cloned in Streptomyces scabies CPS-1,potato scab strain.Analysis results showed that the length of open reading frame(ORF)for nec1 gene was 666 bp,and the GC content was 54.2%.Sequence alignment indicated that a 650 bp up-stream sequence shared 91% similarity with IS 256 family transposase nucleotide sequences by BLASTn searches against GenBank.The segments obtained by PCR amplification were digested by enzymes SphⅠand SacⅠ,and linked to the expression vector pIJ702.The recombinants were transformed into nonpathogenicity strain Streptomyces lividans 66 TK24.Bioas-say results suggested that the transformants possessed the same symptoms as pathogenic strain on potato tuber slices and radish seedlings,which implied that nec1 gene was associated with the pathogenicity of S.scabies CPS-1.展开更多
文摘目的筛选人源膜表面黏附分子NECL1蛋白胞内区相互作用蛋白。方法构建含人NECL1蛋白胞内区氨基酸编码序列的诱饵质粒pGBKT7N-ECL1C,对人胎脑cDNA文库进行酵母双杂交筛选。用GST pu ll down实验进行体外蛋白相互作用的验证。结果酵母双杂交阳性克隆测序后显示共存在9段不同序列(存在重复克隆)。比对氨基酸序列得到5个可能相互作用蛋白。通过GST pu ll down实验验证了其中两个蛋白与NECL1胞内区的相互作用。结论应用酵母双杂交系统,获得了一些候选的NECL1胞内区相互作用蛋白。
文摘Non-equal channel lateral extrusion（NECLE） is a new process that can be used to attain higher grain refinement in comparison with equal channel lateral extrusion（ECLE）. The die design for this process was numerically and experimentally studied. After finding a good correlation between the numerical and experimental results, more comprehensive FE analyses were carried out. Different die geometrical parameters were considered and their effects on the induced plastic strain, stress distribution, velocity field and forming load of the process were investigated. It was found that by this process with a suitable set of die geometrical parameters, higher induced effective strain and more homogeneous strain distribution could be achieved in comparison with ECLE operation.
文摘A pathogenic-related gene nec1 was cloned in Streptomyces scabies CPS-1,potato scab strain.Analysis results showed that the length of open reading frame(ORF)for nec1 gene was 666 bp,and the GC content was 54.2%.Sequence alignment indicated that a 650 bp up-stream sequence shared 91% similarity with IS 256 family transposase nucleotide sequences by BLASTn searches against GenBank.The segments obtained by PCR amplification were digested by enzymes SphⅠand SacⅠ,and linked to the expression vector pIJ702.The recombinants were transformed into nonpathogenicity strain Streptomyces lividans 66 TK24.Bioas-say results suggested that the transformants possessed the same symptoms as pathogenic strain on potato tuber slices and radish seedlings,which implied that nec1 gene was associated with the pathogenicity of S.scabies CPS-1.
