Objective:To investigate the relationship among Pokemon,NF-κB p65 and Bcl-2 in hepatoma cells.Methods:HCC cell HepG2,SMMC7721 and human fetal liver cell line L02 cells were used,and expression of Pokemon,NF-κB p65 a...Objective:To investigate the relationship among Pokemon,NF-κB p65 and Bcl-2 in hepatoma cells.Methods:HCC cell HepG2,SMMC7721 and human fetal liver cell line L02 cells were used,and expression of Pokemon,NF-κB p65 and Bcl-2 in three cells were detected by realtime PCR and western blot.Then siRNA of Pokemon was applied to inhibit the expression of Pokemon and NF-κB p65 and apoptotic rate was determined by flow cytometric analysis. Results:Expressions of Pokemon,NF-κB p65 and Bcl-2 in human hepatoma cell HepG2, SMMC7721 expression were significantly higher than those in human embryonic stem cells L02. siRNA of Pokemon inhibited the expression of Pokemon,NF-κB p65 and Bcl-2 in liver cancer cells,and significantly increased apoptosis of liver cells.While siRNA of NF-κB n65 inhibited the expression of NF-κB p65 and Bcl-2,but Pokemon expression in hepatoma cells had no significant change.Conclusions:The proto-oncogene Pokemon can inhibit PI4ARF by specific transcription regulation of cell cycle and can induce tumors.In addition,Pokemon can regulate NF-κB p65 through the expression of apoptosis repressor,and promote the development of liver cancer.It suggests signal network in the liver include the regulation of new non-classical NF-κB regulatory pathway.展开更多
目的 评价右美托咪啶对体外循环患者中性粒细胞NF-κB活性的影响.方法 选择期行体外循环下心脏瓣膜置换术患者40例,采用随机数字表法将患者分为右美托咪啶组（D组）及对照组（C组）,每组各20例.D组麻醉诱导后给予右美托咪啶负荷量0.5 u ...目的 评价右美托咪啶对体外循环患者中性粒细胞NF-κB活性的影响.方法 选择期行体外循环下心脏瓣膜置换术患者40例,采用随机数字表法将患者分为右美托咪啶组（D组）及对照组（C组）,每组各20例.D组麻醉诱导后给予右美托咪啶负荷量0.5 u g/kg,泵注给药时间为15min,之后以维持量0.5 u g/（kg＆#183;h）继续泵注维持至手术结束;C组麻醉诱导后给予等量的0.9％氯化钠溶液.记录患者主动脉阻断、体外循环及麻醉维持时间.分别于给药前（T0）、停体外循环后2h（T1）、6h（T2）及12h（T3）,采集桡动脉血样行血气分析,计算呼吸指数（Rl）和氧合指数（OI）,提取中性粒细胞核蛋白,测定NF-κ B DNA结合活性;采用ELISA法测定血浆TNF-α和IL-6水平.结果 与T0比较,T1～T3时两组患者RI升高,OI降低（均P＜0.05）,与C组比较,D组患者T1～T3时RI降低（均P＜0.05）,但两组OI比较差异无统计学意义（P＞0.05）.与C组比较,T1～T3时点D组血浆TNF-α、IL-6水平降低;T2～T3时点中性粒细胞NF-κ B DNA结合活性降低（P＜0.05）.结论 右美托咪啶可抑制体外循环下换瓣患者中性粒细胞NF-κB的激活,有助于减轻体外循环炎性反应,改善患者的肺功能.展开更多
Objective： To study the effect of oxymatrine on the expression of nuclear factor-kappa B（NF-K B） of Human benign epidermal keratinocytes line（HaCaT cells）. Methods：HaCaT cells were cultured with different concen...Objective： To study the effect of oxymatrine on the expression of nuclear factor-kappa B（NF-K B） of Human benign epidermal keratinocytes line（HaCaT cells）. Methods：HaCaT cells were cultured with different concentration of Oxymatrine（10 μ g/ml, 50 μ g/ ml and 100 μg/ml） for 24 h, then 10.5 mol/L Substance P was added to the cells. After 30 min, NF-K B expression in the cells was observed by immunocytochemistry, NF-K B P65 protein expression was evaluated by Western blot, and the mRNA expression of NF-K B P65 was evaluated by reverse transcription polymerase chain reaction（RT-PCR）. The 10.5 mol/L Substance P and culture medium were added to the Substance P group and control group, respectively. Results：In control group, expression rate of positive cells, the expressions of protein and mRNA of NF-K B were all low. In Substance P group, when 10μ mol/L Substance P was added, the expressions were all increased（P 〈 0.05）. But in Oxymatrine groups, the expression rate of positive cells, the expressions of protein and mRNA were all descended in a concentration-dependent manner（P 〈 0.05 or P 〈 0.01）. Conclusion：Oxymatrine can down-regulate the expression of NF-K B of the HaCaT cells and may play an important role in regulating anti-inflammation and immunity.展开更多
文摘Objective:To investigate the relationship among Pokemon,NF-κB p65 and Bcl-2 in hepatoma cells.Methods:HCC cell HepG2,SMMC7721 and human fetal liver cell line L02 cells were used,and expression of Pokemon,NF-κB p65 and Bcl-2 in three cells were detected by realtime PCR and western blot.Then siRNA of Pokemon was applied to inhibit the expression of Pokemon and NF-κB p65 and apoptotic rate was determined by flow cytometric analysis. Results:Expressions of Pokemon,NF-κB p65 and Bcl-2 in human hepatoma cell HepG2, SMMC7721 expression were significantly higher than those in human embryonic stem cells L02. siRNA of Pokemon inhibited the expression of Pokemon,NF-κB p65 and Bcl-2 in liver cancer cells,and significantly increased apoptosis of liver cells.While siRNA of NF-κB n65 inhibited the expression of NF-κB p65 and Bcl-2,but Pokemon expression in hepatoma cells had no significant change.Conclusions:The proto-oncogene Pokemon can inhibit PI4ARF by specific transcription regulation of cell cycle and can induce tumors.In addition,Pokemon can regulate NF-κB p65 through the expression of apoptosis repressor,and promote the development of liver cancer.It suggests signal network in the liver include the regulation of new non-classical NF-κB regulatory pathway.
文摘目的 评价右美托咪啶对体外循环患者中性粒细胞NF-κB活性的影响.方法 选择期行体外循环下心脏瓣膜置换术患者40例,采用随机数字表法将患者分为右美托咪啶组（D组）及对照组（C组）,每组各20例.D组麻醉诱导后给予右美托咪啶负荷量0.5 u g/kg,泵注给药时间为15min,之后以维持量0.5 u g/（kg＆#183;h）继续泵注维持至手术结束;C组麻醉诱导后给予等量的0.9％氯化钠溶液.记录患者主动脉阻断、体外循环及麻醉维持时间.分别于给药前（T0）、停体外循环后2h（T1）、6h（T2）及12h（T3）,采集桡动脉血样行血气分析,计算呼吸指数（Rl）和氧合指数（OI）,提取中性粒细胞核蛋白,测定NF-κ B DNA结合活性;采用ELISA法测定血浆TNF-α和IL-6水平.结果 与T0比较,T1～T3时两组患者RI升高,OI降低（均P＜0.05）,与C组比较,D组患者T1～T3时RI降低（均P＜0.05）,但两组OI比较差异无统计学意义（P＞0.05）.与C组比较,T1～T3时点D组血浆TNF-α、IL-6水平降低;T2～T3时点中性粒细胞NF-κ B DNA结合活性降低（P＜0.05）.结论 右美托咪啶可抑制体外循环下换瓣患者中性粒细胞NF-κB的激活,有助于减轻体外循环炎性反应,改善患者的肺功能.
文摘Objective： To study the effect of oxymatrine on the expression of nuclear factor-kappa B（NF-K B） of Human benign epidermal keratinocytes line（HaCaT cells）. Methods：HaCaT cells were cultured with different concentration of Oxymatrine（10 μ g/ml, 50 μ g/ ml and 100 μg/ml） for 24 h, then 10.5 mol/L Substance P was added to the cells. After 30 min, NF-K B expression in the cells was observed by immunocytochemistry, NF-K B P65 protein expression was evaluated by Western blot, and the mRNA expression of NF-K B P65 was evaluated by reverse transcription polymerase chain reaction（RT-PCR）. The 10.5 mol/L Substance P and culture medium were added to the Substance P group and control group, respectively. Results：In control group, expression rate of positive cells, the expressions of protein and mRNA of NF-K B were all low. In Substance P group, when 10μ mol/L Substance P was added, the expressions were all increased（P 〈 0.05）. But in Oxymatrine groups, the expression rate of positive cells, the expressions of protein and mRNA were all descended in a concentration-dependent manner（P 〈 0.05 or P 〈 0.01）. Conclusion：Oxymatrine can down-regulate the expression of NF-K B of the HaCaT cells and may play an important role in regulating anti-inflammation and immunity.
