Polyhydroxyalkanoates are natural,biodegradable,thermoplastic and sustainable polymers with a huge potential in fabrication of bioresorbable implantable devices for tissue engineering.We describe a comparative evaluat...Polyhydroxyalkanoates are natural,biodegradable,thermoplastic and sustainable polymers with a huge potential in fabrication of bioresorbable implantable devices for tissue engineering.We describe a comparative evaluation of three medium chain length polyhydroxyalkanoates(mcl-PHAs),namely poly(3-hydroxyoctanoate),poly(3-hydroxyoctanoate-co-3-hydoxydecanoate)and poly(3-hydroxyoctanoate-co-3-hydroxydecanoate-co-3-hydroxydodecanoate),one short chain length polyhydroxyalkanoate,poly(3-hydroxybutyrate),P(3HB)and synthetic aliphatic polyesters(polycaprolactone and polylactide)with a specific focus on nerve regeneration,due to mechanical properties of mcl-PHAs closely matching nerve tissues.In vitro biological studies with NG108-15 neuronal cell and primary Schwann cells did not show a cytotoxic effect of the materials on both cell types.All mcl-PHAs supported cell adhesion and viability.Among the three mcl-PHAs,P(3HO-co-3HD)exhibited superior properties with regards to numbers of cells adhered and viable cells for both cell types,number of neurite extensions from NG108-15 cells,average length of neurite extensions and Schwann cells.Although,similar characteristics were observed for flat P(3HB)surfaces,high rigidity of this biomaterial,and FDA-approved polymers such as PLLA,limits their applications in peripheral nerve regeneration.Therefore,we have designed,synthesized and evaluated these materials for nerve tissue engineering and regenerative medicine,the interaction of mcl-PHAs with neuronal and Schwann cells,identifying mcl-PHAs as excellent materials to enhance nerve regeneration and potentially their clinical application in peripheral nerve repair.展开更多
基金funded by the European Community’s Seventh Framework Programme(FP7-NMP-2013-SME-7)for NEURIMP un-der grant agreement no.604450CST also acknowledges funding by the Department of Materials Science and Engineering,Faculty of Engineering,University of Sheffield.IR acknowledges funding from the British Council:Innovative and Collaborative Research Partnerships Grants under Pakistan UK Education Gateway(ICRG-2020)+1 种基金Project No:105:006326/DIISB/007/2021the Engineering and Physical Sciences Research Council(EPSRC)funding for the See More Make More(SM3)project,EP/V012126/1.
文摘Polyhydroxyalkanoates are natural,biodegradable,thermoplastic and sustainable polymers with a huge potential in fabrication of bioresorbable implantable devices for tissue engineering.We describe a comparative evaluation of three medium chain length polyhydroxyalkanoates(mcl-PHAs),namely poly(3-hydroxyoctanoate),poly(3-hydroxyoctanoate-co-3-hydoxydecanoate)and poly(3-hydroxyoctanoate-co-3-hydroxydecanoate-co-3-hydroxydodecanoate),one short chain length polyhydroxyalkanoate,poly(3-hydroxybutyrate),P(3HB)and synthetic aliphatic polyesters(polycaprolactone and polylactide)with a specific focus on nerve regeneration,due to mechanical properties of mcl-PHAs closely matching nerve tissues.In vitro biological studies with NG108-15 neuronal cell and primary Schwann cells did not show a cytotoxic effect of the materials on both cell types.All mcl-PHAs supported cell adhesion and viability.Among the three mcl-PHAs,P(3HO-co-3HD)exhibited superior properties with regards to numbers of cells adhered and viable cells for both cell types,number of neurite extensions from NG108-15 cells,average length of neurite extensions and Schwann cells.Although,similar characteristics were observed for flat P(3HB)surfaces,high rigidity of this biomaterial,and FDA-approved polymers such as PLLA,limits their applications in peripheral nerve regeneration.Therefore,we have designed,synthesized and evaluated these materials for nerve tissue engineering and regenerative medicine,the interaction of mcl-PHAs with neuronal and Schwann cells,identifying mcl-PHAs as excellent materials to enhance nerve regeneration and potentially their clinical application in peripheral nerve repair.
