TCERG1L hypermethylation is a risk factor of diabetic retinopathy in Chinese children with type 1 diabetes

●AIM:To identify the differential methylation sites(DMS)and their according genes associated with diabetic retinopathy(DR)development in type 1 diabetes(T1DM)children.●METHODS:This study consists of two surveys.A total of 40 T1DM children was included in the first survey.Because no participant has DR,retina thinning was used as a surrogate indicator for DR.The lowest 25%participants with the thinnest macular retinal thickness were included into the case group,and the others were controls.The DNA methylation status was assessed by the Illumina methylation 850K array BeadChip assay,and compared between the case and control groups.Four DMS with a potential role in diabetes were identified.The second survey included 27 T1DM children,among which four had DR.The methylation patterns of the four DMS identified by 850K were compared between participants with and without DR by pyrosequencing.●RESULTS:In the first survey,the 850K array revealed 751 sites significantly and differentially methylated in the case group comparing with the controls(|Δβ|>0.1 and Adj.P<0.05),and 328 of these were identified with a significance of Adj.P<0.01.Among these,319 CpG sites were hypermethylated and 432 were hypomethylated in the case group relative to the controls.Pyrosequencing revealed that the transcription elongation regulator 1 like(TCERG1L,cg07684215)gene was hypermethylated in the four T1DM children with DR(P=0.018),which was consistent with the result from the first survey.The methylation status of the other three DMS(cg26389052,cg25192647,and cg05413694)showed no difference(all P>0.05)between participants with and without DR.●CONCLUSION:The hypermethylation of the TCERG1L gene is a risk factor for DR development in Chinese children with T1DM.

Perfect 1-k Matchings of Bipartite Graphs

Let k be a positive integer and G a bipartite graph with bipartition (X,Y). A perfect 1-k matching is an edge subset M of G such that each vertex in Y is incident with exactly one edge in M and each vertex in X is incident with exactly k edges in M. A perfect 1-k matching is an optimal semi-matching related to the load-balancing problem, where a semi-matching is an edge subset M such that each vertex in Y is incident with exactly one edge in M, and a vertex in X can be incident with an arbitrary number of edges in M. In this paper, we give three sufficient and necessary conditions for the existence of perfect 1-k matchings and for the existence of 1-k matchings covering | X |−dvertices in X, respectively, and characterize k-elementary bipartite graph which is a graph such that the subgraph induced by all k-allowed edges is connected, where an edge is k-allowed if it is contained in a perfect 1-k matching.

10μm 1280×1024 HgCdTe中波红外焦平面阵列探测性能提升

对像元尺寸为10μm的1280×1024碲镉汞(HgCdTe)中波红外焦平面阵列的制备技术和性能进行了研究。通过B+注入制备小尺寸n-on-p平面结;采用高平整度HgCdTe外延材料和高精度的倒焊互连技术,实现高的电学连通率;采用多段温度填胶固化和边缘刻蚀工艺减轻HgCdTe器件和读出集成电路(ROICs)之间的热失配,从而降低焦平面器件失效率。在85 K焦平面工作温度下,研制探测器的光谱响应范围为3.67μm至4.88μm,有效像元率高达99.95%,并且探测器组件像元的平均噪声等效温差(NETD)和暗电流密度的平均值分别小于16 mK和2.1×10^(-8)A/cm^(2)。与像元尺寸为15μm的探测器相比,10μm的1280×1024中波红外探测器可获取更加精细的图像,具有更远的识别距离。目前,该技术已成功转移到浙江珏芯微电子有限公司(ZJM)的HgCdTe红外探测器产线。

优质抗旱抗除草剂谷子新品种“金苗K 1”的选育及高产栽培技术

“金苗K 1”是赤峰市农牧科学研究院选育的优质抗旱抗除草剂(烯禾啶)的谷子新品种,2019年在中国作物学会粟类作物专业委员会第十三届优质食用粟品鉴会上被评为国家一级优质米。2016年、2017年在赤峰市12个旗县区进行大面积试验,两年平均产量5959.5 kg·hm^(-2),较对照“赤谷8号”增产3.61%,2018年11月2日通过国家非农作物品种登记,登记编号为GPD谷子(2018)150216。2年联合鉴定试验表明,该品种适宜在春谷生态区内蒙古自治区的赤峰市、兴安盟、通辽市、呼和浩特市,宁夏回族自治区固原市,辽宁省的朝阳市、阜新市,山西省的太原市、忻州市、朔州市,新疆维吾尔自治区的昌吉回族自治州、巴音郭勒蒙古自治州、伊犁哈萨克自治州、博尔塔拉蒙古自治州,河北省的承德市、张家口市,甘肃省的白银市、平凉市≥10℃活动积温2600℃的地区春季种植。

Decreased Expression of Vitamin K Epoxide Reductase Complex Subunit 1 in Kidney of Patients with Calcium Oxalate Urolithiasis

Urinary prothrombin fragment 1 （UPTFl） is a potent inhibitor of urinary stone formation. UPTF1 exerts such inhibitory effect by effective 7-carboxylation in which vitamin K epoxide reductase complex subunit 1 （VKORC1）, the rate-limiting enzyme, is involved. This study examined the correlation between VKORC1 expression and calcium oxalate urolithiasis. The renal cortex samples were obtained from patients undergoing nephrectomy and then divided into 3 groups： urolithiasis group, control group A [hydronephrosis-without-stone （HWS） group], control group B （normal control group）, The localization and expression of VKORC1 in renal tissues were determined by using immunohistochemistry, immunofluorescence microscopy, Western blotting and SYBR Green I real-time reverse-transcription PCR. The rapid amplification of cDNA ends （RACE） were conducted to obtain the 3＇- and 5＇-untranslated region （UTR） of VKORC1. The results showed that VKORC1 was located in the cytoplasm of renal tubular epithelial cells. The expression of VKORC1 in the uro- lithiasis group was significantly lower than that in the other two control groups （P〈0.05）. Moreover, the 3＇- and 5＇-UTR sequence of the VKORC1 gene was successfully cloned. No insertion or deletion was found in the 3＇- and 5＇-UTR. However, a 171-bp new base sequence was discovered in the up- stream of 5＇-UTR end in the urolithiasis group. It was concluded that the decreased expression of VKORC 1 may contribute to the development of calcium oxalate urolithiasis in the kidney.

婴儿维生素K_1 10次口服预防效果评价

【目的】 评价婴儿维生素K干预效果。 【方法】 采集七省活产婴儿脐血 5 46份用ELISA法测定PIVKA Ⅱ浓度。对七省活产婴儿 1975 1人整群随机抽样分为婴儿预防组 (VitK12mg 10次 ,口服 )和对照组 (不使用VitK1) ,所有观察对象检测婴儿至满 6月。观察期间预防组及对照组的全部出血病例 ,维生素K缺乏出血 ,及其死亡和残疾病例全部以专门表格登记。 【结果】 婴儿VitK1预防组 10 418例 ,4例发生出血 ,其中 2例为维生素K缺乏出血 ,无死亡 ,无残疾 ,无 1例晚发性维生素K缺乏出血病例。对照组 93 3 3人 ,5 2例发生出血 ,其中 3 0例维生素K缺乏出血 (2 1例晚发性VitK缺乏 ) ,15例死亡 ,3例残疾 ,其中 9例死亡 ,3例残疾由于维生素K缺乏。婴儿预防组脐血、1月及 2月血浆PIVKA Ⅱ阳性率分别为 70 .0 %、0 .0 %、0 .0 % ,对照组为 76.2 %、2 8.6%、14 .3 %。 【结论】 婴儿口服VitK预防取得了显著效果。

T.spelta 1BS染色体导入K型小麦的效应

T.spelta1 BS染色体导入 K型小麦不育系 (K3 3 1 4A) ,育成非 1 B/1 R类型 K 型小麦不育系KTSP3 3 1 4A,研究其遗传效应。结果表明 ,T.spelta1 BS染色体导入使非 1 B/1 R类型 K型小麦不育系更易恢复 ;不育系本身不产生单倍体 ,其杂交种 F1 产生极少或不产生单倍体 ;农艺性状除了株高具有明显的优势外 。

RNAi沉默Beclin 1基因对维生素K_3损伤人肝癌细胞SMMC-7721的影响

目的:探讨应用RNAi技术沉默Beclin1基因对维生素(vitamin K3,VitK3)引起的人肝癌SMMC-7721细胞损伤的影响。方法:应用真核细胞转染技术将Psilencer3.1-siRNA-Beclin1重组质粒转入人肝癌SMMC-7721细胞,同时分别设立转染空质粒阴性对照组和转染试剂阴性对照。于48h后收集细胞,分别提取细胞总RNA及总蛋白,通过RT-PCR和Western blotting检测Beclin1基因表达。应用40μmol/LVitK3作用Beclin1-siRNA细胞株,Hoechst33342染色检测细胞凋亡率。结果:与对照组相比,siRNA重组质粒明显降低Beclin1 mRNA水平,抑制其蛋白表达。40μmol/LVitK3作用人肝癌SMMC-7721细胞Beclin1 mRNA表达明显高于对照组,细胞凋亡率增加(P<0.01);而Beclin1-siRNA细胞株,Beclin1 mRNA表达显著低于对照组,与40μmol/LVitK3作用组相比细胞凋亡率显著增加(P<0.01)。结论:Psilencer3.1-siRNA-Beclin1转染人肝癌SMMC-7721细胞后,可有效抑制Beclin-1 mRNA和蛋白的表达,抑制VitK3引起的Beclin1依赖性细胞自噬信号通路的激活,促进细胞凋亡。

自噬基因Beclin 1在SKOV3细胞中的表达及其调控相关信号传导途径的研究

目的探讨自噬基因Beclin 1在人卵巢癌SKOV3细胞中过表达后自噬相关信号调控通路PI3K/PKB途径中ClassⅠPI3K(p110α)、p-PKB和ClassⅢPI3K(hvps34)的表达变化以及对肿瘤细胞的自噬活性的影响.方法将自噬基因Beclin 1的真核表达载体pcDNA3.1/Beclin1转染SKOV3细胞;分别用荧光定量RT-PCR和Western blot在mRNA和蛋白水平检测用流式细胞仪检测Beclin1、p110α、hvps34和p-PKB的表达;在电镜和荧光显微镜下观察自噬现象,用流式细胞仪检肿瘤细胞自噬情况.结果 pcDNA3.1/Beclin1转染后SKOV3细胞Beclin1表达在mRNA和蛋白质水平明显高于转染空质粒pcDNA3.1和未转染细胞;PcDNA3.1/Beclin1转染后hvps34表达上调,而p110α、p-PKB的表达下调.PcDNA3.1/Beclin1转染SKOV3细胞后在在电镜下可见大量自噬囊泡形成.在荧光显微镜下见pcDNA3.1/Beclin1转染后SKOV3细胞中MDC阳性细胞明显增加.流式细胞仪检测pcDNA3.1/Beclin1转染后SKOV3细胞MDC平均荧光强度高于pcDNA3.1转染组和未转染组.Beclin 1在SKOV3中的过表达后抑制肿瘤细胞在体外的生长,抑制率为58.68%.结论自噬基因Beclin1在人卵巢癌细胞SKOV3中过表达可使ClassⅢPI3K(hvps34)表达上调,ClassⅠPI3K(p110α)及其下游的p-PKB表达下调,诱导肿瘤细胞自噬活性增加,抑制SKOV3在体外的增殖.

Rosmarinic acid elicits neuroprotection in ischemic stroke via Nrf2 and heme oxygenase 1 signaling

Rosmarinic acid（RA） can elicit a neuroprotective effect against ischemic stroke, but the precise molecular mechanism remains poorly understood. In this study, an experimental ischemic stroke model was established in CD-1 mice（Beijing Vital River Laboratory Animal Technology, Beijing, China） by occluding the right middle cerebral artery for 1 hour and allowing reperfusion for 24 hours. After intraperitoneally injecting model mice with 10, 20, or 40 mg/kg RA, functional neurological deficits were evaluated using modified Longa scores. Subsequently, cerebral infarct volume was measured using TTC staining and ischemic brain tissue was examined for cell apoptosis with TUNEL staining. Superoxide dismutase activity and malondialdehyde levels were measured by spectrophometry. Expression of heme oxygenase-1（HO-1）, nuclear factor erythroid 2-related factor 2（Nrf2）, Bcl-2, Bax, Akt, and phospho-Ser473 Akt proteins in ischemic brain tissue was detected by western blot, while mRNA levels of Nrf2, HO-1, Bcl-2, and Bax were analyzed using real time quantitative PCR. In addition, HO-1 enzyme activity was measured spectrophotometrically. RA（20 and 40 mg/kg） greatly improved neurological function, reduced infarct volume, decreased cell apoptosis, upregulated Bcl-2 protein and mRNA expression, downregulated Bax protein and mRNA expression, increased HO-1 and Nrf2 protein and mRNA expression, increased superoxide dismutase activity, and decreased malondialdehyde levels in ischemic brain tissue of model mice. However, intraperitoneal injection of a HO-1 inhibitor（10 mg/kg zinc protoporphyrin IX） reversed the neuroprotective effects of RA on HO-1 enzyme activity and Bcl-2 and Bax protein expression. The PI3 K/Akt signaling pathway inhibitor LY294002（10 mM） inhibited Akt phosphorylation, as well as Nrf2 and HO-1 expression. Our findings suggest that RA has anti-oxidative and anti-apoptotic properties that protect against ischemic stroke by a mechanism involving upregulation of Nrf2 and HO-1 expression via the PI3 K/Akt signaling pathway.

Lysine-specific demethylase 1 inhibitor rescues the osteogenic ability of mesenchymal stem cells under osteoporotic conditions by modulating H3K4 methylation

Bone tissue engineering may be hindered by underlying osteoporosis because of a decreased osteogenic ability of autologous seed cells and an unfavorably changed microenvironment in these patients. Epigenetic regulation plays an important role in the developmental origins of osteoporosis; however, few studies have investigated the potential of epigenetic therapy to improve or rescue the osteogenic ability of bone marrow mesenchymal stem cells(BMMSCs) under osteoporotic conditions. Here, we investigated pargyline, an inhibitor of lysine-specific demethylase 1(LSD1), which mainly catalyzes the demethylation of the di- and mono-methylation of H3K4. We demonstrated that 1.5 mmol·Lpargyline was the optimal concentration for the osteogenic differentiation of human BMMSCs. Pargyline rescued the osteogenic differentiation ability of mouse BMMSCs under osteoporotic conditions by enhancing the dimethylation level of H3K4 at the promoter regions of osteogenesis-related genes. Moreover, pargyline partially rescued or prevented the osteoporotic conditions in aged or ovariectomized mouse models, respectively. By introducing the concept of epigenetic therapy into the field of osteoporosis, this study demonstrated that LSD1 inhibitors could improve the clinical practice of MSC-based bone tissue engineering and proposes their novel use to treat osteoporosis.

Baicalin protects neonatal rat brains against hypoxicischemic injury by upregulating glutamate transporter 1 via the phosphoinositide 3-kinase/protein kinase B signaling pathway

Baicalin is a flavonoid compound extracted from Scutellaria baicalensis root.Recent evidence indicates that baicalin is neuroprotective in models of ischemic stroke.Here,we investigate the neuroprotective effect of baicalin in a neonatal rat model of hypoxic-ischemic encephalopathy.Seven-day-old pups underwent left common carotid artery ligation followed by hypoxia（8% oxygen at 37°C） for 2 hours,before being injected with baicalin（120 mg/kg intraperitoneally） and examined 24 hours later.Baicalin effectively reduced cerebral infarct volume and neuronal loss,inhibited apoptosis,and upregulated the expression of p-Akt and glutamate transporter 1.Intracerebroventricular injection of the phosphoinositide 3-kinase/protein kinase B（PI3 K/Akt） inhibitor LY294002 30 minutes before injury blocked the effect of baicalin on p-Akt and glutamate transporter 1,and weakened the associated neuroprotective effect.Our findings provide the first evidence,to our knowledge that baicalin can protect neonatal rat brains against hypoxic-ischemic injury by upregulating glutamate transporter 1 via the PI3 K/Akt signaling pathway.

Inositol 1,4,5-trisphosphate 3-kinases:functions and regulations

Inositol 1,4,5-trisphosphate 3-kinase (IP3 3-kinase/IP3K) plays an important role in signal transduction in animal cellsby phosphorylating inositol 1,4,5-trisphosphate (IP3) to inositol 1,3,4,5-tetrakisphosphate (IP4). Both IP3 and IP4 arecritical second messengers which regulate calcium (Ca2+) homeostasis. Mammalian IP3Ks are involved in many biologicalprocesses, including brain development, memory, learning and so on. It is widely reported that Ca2+ is a canonicalsecond messenger in higher plants. Therefore, plant IP3K should also play a crucial role in plant development. Recently,we reported the identification of plant IP3K gene (AtIpk2β/AtIP3K) from Arabidopsis thaliana and its characterization.Here, we summarize the molecular cloning, biochemical properties and biological functions of IP3Ks from animal, yeastand plant. This review also discusses potential functions of IP3Ks in signaling crosstalk, inositol phosphate metabolism,gene transcriptional control and so on.

ACEX 1K系列CPLD的配置

Altera公司近期推出新一代CPLD器件——ACEX 1K,这种芯片具有易失性,需要配置器件来保存CPLD器件的配置数据。对此,介绍了ACEX 1K系列器件的配置方法,对各种配置方法进行了分析对比,并着重论述了应用配置器件配置ACEX 1K系列器件的优点。

ACEX 1K系列CPLD配置方法探讨

介绍ACEX 1K系列器件的配置方法,对几种方法进行了分析对比,并着重论述了应用配置器件配置 ACEX 1K系列器件的优点。

Isolation, Crystal Structure and Na^＋/K^＋-ATPase Inhibitory Activity of 1β-Hydroxydigitoxigenin

The title compound 1β-hydroxydigitoxigenin（1） was isolated from the ethanol extract of the roots of Streptocaulon juventas. The crystal structure of 1, C23H34O5·H2O, was determined by Synchrotron X-ray diffraction analysis due to small crystal size（0.14 mm × 0.04 mm × 0.01 mm）. The crystal belongs to monoclinic, space group P21, with a = 7.6624（15）, b= 13.460（3）, c = 10.370（2） A, b = 92.40（3）°, V = 1068.6（4）A^3, Z = 2, Mr = 406.50, Dx = 1.263 g/cm^3, λ（synchrotron） = 1.2399 A, μ（synchrotron 1.23990） = 0.333 cm^-1, F（000） = 550, S = 1.059, R = 0.0625 and wR = 0.1687 for 4247 unique reflections, of which 3687 were observed（I 〉 2σ（I））. The asymmetric unit contains one independent molecule of 1 and one water molecule which are connected through hydrogen bonds. The conformation of 1 in crystalline state is in good agreement with the solution structure in methanol as indicated by ^1H-NMR analysis. The absolute configuration of 1 could be assigned by referring to the known configuration of the lactone ring at C（17b）. In the solid state, intermolecular hydrogen bonds involving carbonyl group in the lactone moiety and the hydroxyl groups in the steroid moiety ester linked adjacent molecules into a three-dimensional network. Compound 1 showed significant inhibition on Na^＋/K^＋-ATPase with an IC50 of 2.46 mM, which is stronger thiocarbonylbufalin but weaker than a close analog digitoxigenin, suggesting that a lactone ring is important and the substitution of a hydroxyl group at C（1） is not favored for the inhibition of Na^＋/K^＋-ATPase.

皖南某地汉族人群CYP2C9 1075A>C和VKORC1 1639G>A基因多态性研究

目的探讨CYP2C9和VKORC1基因在皖南地区汉族人群中多态性分布。方法选取皖南医学院弋矶山医院2013年8月至2014年8月135例需接受华法林抗凝治疗的患者,采用原位杂交荧光染色分析技术检测患者CYP2C9 1075A>C和VKORC1 1639G>A基因型。结果患者CYP2C9 1075AA基因型94.07%,AC基因型为5.19%,CC基因型为0.74%;VKORC1 1639基因AA型83.71%,GA型14.81%,GG型1.48%。CYP2C91075A等位基因分布频率为96.67%,C等位基因频率为3.33%;VKORC1 1639A等位基因和G等位基因分布频率分别为91.11%和8.89%。CYP2C9 1075AA/VKORC1 1639AA基因组合分布频率为78.52%。结论皖南地区汉族人群CYP2C9和VKORC1基因存在多态性分布,可为华法林个体化抗凝提供用药参考。

The modulation of radiation-induced cell death by genistein in K562 cells:Activation of thymidine kinase 1

Ionizing radiation is one of the most effective tools in cancer therapy. In a previous study, we reported that protein tyrosine kinase (PTK) inhibitors modulate the radiation responses in the human chronic myelogenous leukemia (CML)cell line K562. The receptor tyrosine kinase inhibitor, genistein, delayed radiation-induced cell death, while non-recepter tyrosine kinase inhibitor, herbimycin A (HMA) enhances radiation-induced apoptosis. In this study, we focused on the modulation of radiation-induced cell death by genistein and performed PCR-select suppression subtractive hybridization(SSH) to understand its molecular mechanism. We identified human thymidine kinase 1 (TK1), which is cell cycle regulatory gene and confirmed expression of TK1 mRNA by Northern blot analysis. Expression of TK1 mRNA and TK 1enzymatic activity were parallel in their increase and decrease. TK1 is involved in G1-S phase transition of cell cycle progression. In cell cycle analysis, we showed that radiation induced G2 arrest in K562 cells but it was not able to sustain. However, the addition of genistein to irradiated cells sustained a prolonged G2 arrest up to 120 h. In addition,the expression of cell cycle-related proteins, cyclin A and cyclin B 1, provided the evidences of G1/S progression and G2-arrest, and their relationship with TK1 in cells treated with radiation and genistein. These results suggest that the activation of TK1 may be critical to modulate the radiation-induced cell death and cell cycle progression in irradiated K562 cells.

Relationship between R219K polymorphism of adenosine triphosphate-binding cassette transporter 1 gene and cerebral infarction: A case-controlled analysis

BACKGROUND： Studies have shown that adenosine triphosphate-binding cassette transporter 1 （ABCA1） gene influences atherosclerosis. Studies have also demonstrated that cerebral infarction does not occur often in pre-menopausal women. It has been, therefore, assumed that sex plays a role in R219K polymorphism of ABCA1 gene and cerebral infarction. OBJECTIVE： To explore the relationship between lipid metabolism-correlated R219K polymorphism of ABCA1 gene, risk factors of cerebral infarction and lipid level, and to determine whether there were significant differences in gender between R219K polymorphism of ABCA1 gene and cerebral infarction. DESIGN, TIME AND SETTING： A multicentral and non-randomized, controlled study based on gene polymorphism was performed at the Chinese National Human Genome Center, and lipid concentrations were measured at Beijing Xuanwu Hospital. Patients with cerebral infarction and healthy subjects were enrolled from eight hospitals of six provinces of China between October 2002 and December 2004. PARTICIPANTS： There were 177 patients in the cerebral infarction group, including 119 males and 58 females, with a mean age of （60 -＋ 13） years, and 234 healthy subjects in the normal control group, including 79 males and 155 females, with a mean age of （58 ± 12） years. METHODS： R219K polymorphism of the ABCA1 gene was detected using polymerase chain reaction-restriction fragment length polymorphism, and blood lipid concentrations were simultaneously measured. MAIN OUTCOME MEASURES： Genotype and allele frequency of R219K polymorphic site, and blood lipid concentrations. RESULTS： RR genotype and R allele frequency of males in the cerebral infarction were significantly greater than males in the normal control group [RR genotype： x2 = 5.305, OR （95% CO, 2.326 （1.120 4.828）, P〈 0.05; R allele： x2= 4.219, OR （95% CO, 1.528 （1.019 2.292）, P〈 0.05]. In addition, RR genotype and R allele frequency of males were significantly greater than females in the cerebral infarction group [RR genotype： x2= 5.172, OR （95% C/）, 2.604 （1.120-6.057）, P〈 0.05; R allele： x2= 4.818, OR （95% CO, 1.652 （1.053 2.589）, P〈 0.05]. There were no significant differences between genotype and lipid concentrations between the two groups （P〉 0.05）. CONCLUSION： The RR genotype of ABCA1 R219K might be associated with onset of cerebral infarction in males, but blood lipid concentrations do not relate to R219K polymorphism.

Isolation and Identification of Alkali-Resistant 1,3-Propanediol Producing Strain

1,3-Propanediol is a promising renewable resource produced by microbial production. It is mainly used in many synthetic reactions, particularly applied to the polymer synthesis and cosmetics industry. We described here the isolation of strain ZH-1, which has the ability of high production with 1,3-propanediol, from Fenhe River in China. It was classified as a member of K. pneumoniae after the study of phenotypic, physio-logical, biochemical and phylogenetic (16S rDNA). The initial glycerol concentration, fermentation time and pH value of strain ZH-1 were determined to be 50 g·L-1, 36 h and 8.0. Under these conditions, the practical yield of 1,3-PD was 18.53 g·L-1 and a molar yield (mol1,3-PD molGlycerol-1 of 1,3-propanediol to glycerol of 0.497. In addition, we found that for the strain ZH-1, the optimum grown pH was 9.0, so we can deter-mine that it is a new member of alkali-resistant strains.