The lignocellulosic crop Miscanthus spp.has been identified as a good candidate for biomass production.The responses of Miscanthus sinensis Anderss.to salinity were studied to satisfy the needs for high yields in marg...The lignocellulosic crop Miscanthus spp.has been identified as a good candidate for biomass production.The responses of Miscanthus sinensis Anderss.to salinity were studied to satisfy the needs for high yields in marginal areas and to avoid competition with food production.The results indicated that the relative advantages of the tolerant accession over the sensitive one under saline conditions were associated with restricted Na^(+)accumulation in shoots.Seedlings of two accessions(salt-tolerant‘JM0119’and salt-sensitive‘JM0099’)were subjected to 0(control),100,200,and 300 mM NaCl stress to better understand the salt-induced biochemical responses of genes involved in Na^(+)accumulation in M.sinensis.The adaptation responses of genes encoding for Na^(+)/H^(+)antiporters,NHX1 and SOS1 to NaCl stress were examined in JM0119 and JM0099.The cDNA sequences of genes examined were highly conserved among the relatives of M.sinensis based on the sequencing on approximate 600 bp-long cDNA fragments obtained from degenerate PCR.These salt-induced variations of gene expression investigated by quantitative real-time PCR provided evidences for insights of the molecular mechanisms of salt tolerance in M.sinensis.The expression of NHX1 was up-regulated by salt stress in JM0119 shoot and root tissues.However,it was hardly affected in JM0099 shoot tissue except for a significant increase at the 100 mM salt treatment,and it was salt-suppressed in the JM0099 root tissue.In the root tissue,the expression of SOS1 was induced by the high salt treatment in JM0119 but repressed by all salt treatments in JM0099.Thus,the remarkably higher expression of NHX1 and SOS1 were associated with the resistance to Na^(+)toxicity by regulation of the Na^(+)influx,efflux,and sequestration under different salt conditions.展开更多
盐分是降低作物产量的主要非生物胁迫因素.甜菜(Beta vulgaris)是耐盐性较强的经济作物,研究甜菜的耐盐机制具有重要意义.本研究以栽培甜菜O68品系为试验材料,利用m^(6)A-IP-qPCR探究了盐胁迫下m^(6)A修饰对甜菜耐盐基因CMO和NHX1表达...盐分是降低作物产量的主要非生物胁迫因素.甜菜(Beta vulgaris)是耐盐性较强的经济作物,研究甜菜的耐盐机制具有重要意义.本研究以栽培甜菜O68品系为试验材料,利用m^(6)A-IP-qPCR探究了盐胁迫下m^(6)A修饰对甜菜耐盐基因CMO和NHX1表达的调控作用.在300 mM NaCl处理下,叶片中甘氨酸甜菜碱和Na+含量增加,编码CMO和NHX1的mRNA的表达水平升高、m^(6)A修饰水平降低、稳定性增强.这为m^(6)A修饰调控作物耐盐性提供了新的见解.展开更多
基金This study was supported by grants from Shenzhen Fundamental Research Program(Grant Nos.JCYJ20170818140058675 and JCYJ20170818140127741)Natural Science Foundation of Top Talent of SZTU(Grant Nos.2019010801010 and 2019010801009).
文摘The lignocellulosic crop Miscanthus spp.has been identified as a good candidate for biomass production.The responses of Miscanthus sinensis Anderss.to salinity were studied to satisfy the needs for high yields in marginal areas and to avoid competition with food production.The results indicated that the relative advantages of the tolerant accession over the sensitive one under saline conditions were associated with restricted Na^(+)accumulation in shoots.Seedlings of two accessions(salt-tolerant‘JM0119’and salt-sensitive‘JM0099’)were subjected to 0(control),100,200,and 300 mM NaCl stress to better understand the salt-induced biochemical responses of genes involved in Na^(+)accumulation in M.sinensis.The adaptation responses of genes encoding for Na^(+)/H^(+)antiporters,NHX1 and SOS1 to NaCl stress were examined in JM0119 and JM0099.The cDNA sequences of genes examined were highly conserved among the relatives of M.sinensis based on the sequencing on approximate 600 bp-long cDNA fragments obtained from degenerate PCR.These salt-induced variations of gene expression investigated by quantitative real-time PCR provided evidences for insights of the molecular mechanisms of salt tolerance in M.sinensis.The expression of NHX1 was up-regulated by salt stress in JM0119 shoot and root tissues.However,it was hardly affected in JM0099 shoot tissue except for a significant increase at the 100 mM salt treatment,and it was salt-suppressed in the JM0099 root tissue.In the root tissue,the expression of SOS1 was induced by the high salt treatment in JM0119 but repressed by all salt treatments in JM0099.Thus,the remarkably higher expression of NHX1 and SOS1 were associated with the resistance to Na^(+)toxicity by regulation of the Na^(+)influx,efflux,and sequestration under different salt conditions.
文摘盐分是降低作物产量的主要非生物胁迫因素.甜菜(Beta vulgaris)是耐盐性较强的经济作物,研究甜菜的耐盐机制具有重要意义.本研究以栽培甜菜O68品系为试验材料,利用m^(6)A-IP-qPCR探究了盐胁迫下m^(6)A修饰对甜菜耐盐基因CMO和NHX1表达的调控作用.在300 mM NaCl处理下,叶片中甘氨酸甜菜碱和Na+含量增加,编码CMO和NHX1的mRNA的表达水平升高、m^(6)A修饰水平降低、稳定性增强.这为m^(6)A修饰调控作物耐盐性提供了新的见解.
