GmACP expression is decreased in Gm NORK knockdown transgenic soybean roots

NORK and soybean acyl carrier protein(ACP) both play important roles in nodulation.However,the relationship between Nod factor signaling and fatty acid(FA) biosynthesis during symbiotic development is unknown.In this study,an RNAi plasmid of Gm NORK was constructed and transformed into soybean roots by Agrobacterium rhizogene-mediated hairy-root transformation.The nodule number decreased substantially in Gm NORK knockdown soybean transgenic roots.To investigate the relationship between Gm ACP and Nod factor signaling,we measured Gm ACP expression levels in Gm NORK RNAi soybean transgenic roots and found that rhizobia inoculation led to substantially reduced Gm ACP expression.Thus,FA biosynthesis was affected by Nod factor signaling during nodule development in soybean,a finding that provides valuable information that improves our understanding of the functions of Gm NORK and Gm ACP in symbiotic signaling and nodule development.

紫云英结瘤受体激酶靶蛋白的筛选与鉴定

利用酵母双杂交技术,以结瘤受体激酶(NORK)蛋白的跨膜结构域(TM)为诱饵,进行了与其相互作用靶蛋白的筛选鉴定。结果获得41个候选阳性克隆,通过测序和在NCBI中进行Blast比对分析,发现其中一个具有重要生物学功能的互作蛋白翻译延伸因子AsEF1-α,并将该蛋白相应的编码基因命名为AsEF1-α。进一步的验证表明靶蛋白AsEF1-α与诱饵互作的关键结构域是EF1-α-Ⅲ。

影响CFB锅炉长周期运行的因素分析

从安装、运行、检修和技改的角度分析了影响燃烧劣质煤的循环流化床锅炉长周期运行的因素。探讨了促使CFB锅炉得到更快的推广并向大容量机组的方向发展的问题。