网络时间协议(NTP,Network Time Protocol)是一种基于UDP的时间同步协议。文章介绍了NTP协议的基本原理,对时间同步误差进行了简要的分析。对比不同NTP实现方式,针对Unix平台下的NTPD(Network Time Protocol Daemen)服务的配置和行为模...网络时间协议(NTP,Network Time Protocol)是一种基于UDP的时间同步协议。文章介绍了NTP协议的基本原理,对时间同步误差进行了简要的分析。对比不同NTP实现方式,针对Unix平台下的NTPD(Network Time Protocol Daemen)服务的配置和行为模式进行了透彻的分析研究,并提供了Windows、Unix、网络设备等不同平台下NTP服务典型配置。根据电力企业的应用需求,考虑到经济性和灵活性方面的要求,提出了层次化的NTP服务架构体系建设方案,在安徽电力信息网进行了实施,取得了良好的应用效果。展开更多
Virus-induced gene silencing(VIGS)is a natural defense mechanism of plants,which can cause sequence-specific degradation of viral RNA and is often used for studying the functional genome.At present,the VIGS system med...Virus-induced gene silencing(VIGS)is a natural defense mechanism of plants,which can cause sequence-specific degradation of viral RNA and is often used for studying the functional genome.At present,the VIGS system mediated by Tobacco rattle virus(TRV)has been successfully established in many plant species.However,the VIGS system in Chinese narcissus had not been reported yet.In this paper,the first use of the TRV-VIGS system in Chinese narcissus is described in detail.By injecting pTRV2-GFP into narcissus leaves,we confirmed that TRV could infect narcissus and move in narcissus plants.After the inoculation of pTRV2-Nt PDS by two methods,phenomena such as photo-bleaching appeared in narcissus leaves and qRT-PCR results proved that PDS gene of narcissus was successfully silenced.Besides,pTRV2-NtMYB3 was inoculated into basal plates of narcissus to study the function of NtMYB3;the results showed that NtMYB3 is an inhibitor of flavonoids biosynthesis,which can increase the content of proanthocyanidins in basal plates of narcissus by repressing the expression level of NtFLS.These results indicate that the TRV-VIGS system has been successfully established in Chinese narcissus and successfully applied to the functional study of NtMYB3.展开更多
文摘网络时间协议(NTP,Network Time Protocol)是一种基于UDP的时间同步协议。文章介绍了NTP协议的基本原理,对时间同步误差进行了简要的分析。对比不同NTP实现方式,针对Unix平台下的NTPD(Network Time Protocol Daemen)服务的配置和行为模式进行了透彻的分析研究,并提供了Windows、Unix、网络设备等不同平台下NTP服务典型配置。根据电力企业的应用需求,考虑到经济性和灵活性方面的要求,提出了层次化的NTP服务架构体系建设方案,在安徽电力信息网进行了实施,取得了良好的应用效果。
基金the Science and technology innovation fund of Fujian Agriculture and Forestry University(Grant Nos.KFA17352A,KFA17602A)。
文摘Virus-induced gene silencing(VIGS)is a natural defense mechanism of plants,which can cause sequence-specific degradation of viral RNA and is often used for studying the functional genome.At present,the VIGS system mediated by Tobacco rattle virus(TRV)has been successfully established in many plant species.However,the VIGS system in Chinese narcissus had not been reported yet.In this paper,the first use of the TRV-VIGS system in Chinese narcissus is described in detail.By injecting pTRV2-GFP into narcissus leaves,we confirmed that TRV could infect narcissus and move in narcissus plants.After the inoculation of pTRV2-Nt PDS by two methods,phenomena such as photo-bleaching appeared in narcissus leaves and qRT-PCR results proved that PDS gene of narcissus was successfully silenced.Besides,pTRV2-NtMYB3 was inoculated into basal plates of narcissus to study the function of NtMYB3;the results showed that NtMYB3 is an inhibitor of flavonoids biosynthesis,which can increase the content of proanthocyanidins in basal plates of narcissus by repressing the expression level of NtFLS.These results indicate that the TRV-VIGS system has been successfully established in Chinese narcissus and successfully applied to the functional study of NtMYB3.
