A new type of NaCS/PDMDAAC polyelectrolyte complex membrane prepared by interfacial reaction of sodium cellulose sulfate as polyanions and poly(dimethylallylammonium chloride)as polycations was proposed.The static con...A new type of NaCS/PDMDAAC polyelectrolyte complex membrane prepared by interfacial reaction of sodium cellulose sulfate as polyanions and poly(dimethylallylammonium chloride)as polycations was proposed.The static contact angle of the polyelectrolyte complex(PELC)membrane surface was measured to characterize membrane wettability.The effects of concentration of polyelectrolyte,molecular weight,and reaction time on contact angle were investigated.It was found that NaCS/PDMDAAC PELC membrane was hydrophilic.Its contact angle decreased with the increasing concentration of NaCS and molecular weight of PDMDAAC,and increased with the increasing concentration of PDMDAAC and reaction time.展开更多
The production of ATE(anti-thrombus enzyme) from a kind of Bacillus subtilis immobilized in NaCS-PDMDAAC microcapsules was studied.The influence of mean diameter of microcapsules, concentration of PDMDAAC and the comp...The production of ATE(anti-thrombus enzyme) from a kind of Bacillus subtilis immobilized in NaCS-PDMDAAC microcapsules was studied.The influence of mean diameter of microcapsules, concentration of PDMDAAC and the components of culture medium on the production of ATE in the condition of encapsulation culture was studied. In shaking flasks, the maximum enzyme activity in microcapsules was 1850 UK·ml -1 when the mean diameter of microcapsules was 1.60 mm, the concentration of PDMDAAC was 2.0% and the culture medium was LB culture medium(Tryptone)+glucose(0.5%)+phosphate buffer(100 ml).A bubbling culture system was designed. In this system, the maximum enzyme activity in microcapsules was 2050 UK·ml -1 , which was 3.6 times that in the condition of suspension fermentation; the maximum cell density in microcapsules was 4.89×10 9cells·ml -1 , which was 2.7 times that in the condition of suspension fermentation.展开更多
In order to produce macromolecules by using the immobilized cells in microcapsules, a novel macro-porous NaCS-PDMDAAC capsules system was used to immobilize Candida valida in a semi-continuous culture mode for produci...In order to produce macromolecules by using the immobilized cells in microcapsules, a novel macro-porous NaCS-PDMDAAC capsules system was used to immobilize Candida valida in a semi-continuous culture mode for producing lipase. It was found that the encapsulation system had no negative effect on the growth and lipase productivity, and the lipase produced inside capsules could diffuse freely to the media outside capsules. The cultivation with the capsules was carried out for 15 batches and the lipase in each batch had almost the same activity. In comparison with free cell cultivation, the immobilized cell fermentation shortened obviously the period of glucose consumption from 24 hours to 8 hours and increased lipase productivity.展开更多
文摘A new type of NaCS/PDMDAAC polyelectrolyte complex membrane prepared by interfacial reaction of sodium cellulose sulfate as polyanions and poly(dimethylallylammonium chloride)as polycations was proposed.The static contact angle of the polyelectrolyte complex(PELC)membrane surface was measured to characterize membrane wettability.The effects of concentration of polyelectrolyte,molecular weight,and reaction time on contact angle were investigated.It was found that NaCS/PDMDAAC PELC membrane was hydrophilic.Its contact angle decreased with the increasing concentration of NaCS and molecular weight of PDMDAAC,and increased with the increasing concentration of PDMDAAC and reaction time.
文摘The production of ATE(anti-thrombus enzyme) from a kind of Bacillus subtilis immobilized in NaCS-PDMDAAC microcapsules was studied.The influence of mean diameter of microcapsules, concentration of PDMDAAC and the components of culture medium on the production of ATE in the condition of encapsulation culture was studied. In shaking flasks, the maximum enzyme activity in microcapsules was 1850 UK·ml -1 when the mean diameter of microcapsules was 1.60 mm, the concentration of PDMDAAC was 2.0% and the culture medium was LB culture medium(Tryptone)+glucose(0.5%)+phosphate buffer(100 ml).A bubbling culture system was designed. In this system, the maximum enzyme activity in microcapsules was 2050 UK·ml -1 , which was 3.6 times that in the condition of suspension fermentation; the maximum cell density in microcapsules was 4.89×10 9cells·ml -1 , which was 2.7 times that in the condition of suspension fermentation.
文摘In order to produce macromolecules by using the immobilized cells in microcapsules, a novel macro-porous NaCS-PDMDAAC capsules system was used to immobilize Candida valida in a semi-continuous culture mode for producing lipase. It was found that the encapsulation system had no negative effect on the growth and lipase productivity, and the lipase produced inside capsules could diffuse freely to the media outside capsules. The cultivation with the capsules was carried out for 15 batches and the lipase in each batch had almost the same activity. In comparison with free cell cultivation, the immobilized cell fermentation shortened obviously the period of glucose consumption from 24 hours to 8 hours and increased lipase productivity.
