In this study,we investigated the optimal concentration of nutrient-mist and NaHSO3 for cutting propagation of Juniperus squamata ’Blue Star’,as well as the variation in the contents of chlorophyll in leaves,and sol...In this study,we investigated the optimal concentration of nutrient-mist and NaHSO3 for cutting propagation of Juniperus squamata ’Blue Star’,as well as the variation in the contents of chlorophyll in leaves,and solubility carbohydrate,soluble protein and other nutrition in leaves and bases of cuttings during rooting.Cuttings of grade Ⅲ(the lenghth of cutting bases lignified from 0.1 to 0.2 cm) was treated by 1/4MS+NaHSO3(200 mg·L-1),and the rooting rate rose 35% and root qualities were improved compared with control experiment,for there were different effects among three grade cuttings.The treatment of grade Ⅲ cuttings also resulted in higher content of soluble sugar and starch of those bases during rooting,especially obviously different for fifteen days,but less effect on the chlorophyll and some nutrition content of cutting leaves.展开更多
: Spraying a 1–2 mmol/L solution of NaHSO3 on the leaves of wild-type rice (Oryza sativa L.) Kitaake (WT), phosphoenolpyruvate carboxylase (PEPC) transgenic (PC) rice and PEPC+phosphate dikinase (PPDK) transgenic ric...: Spraying a 1–2 mmol/L solution of NaHSO3 on the leaves of wild-type rice (Oryza sativa L.) Kitaake (WT), phosphoenolpyruvate carboxylase (PEPC) transgenic (PC) rice and PEPC+phosphate dikinase (PPDK) transgenic rice (PC+PK), in which the germplasm was transformed with wild-type Kitaake as the gene receptor, resulted in an enhancement of the net photosynthetic rate by 23.0%, 28.8%, and 34.4%, respectively, for more than 3 d. It was also observed that NaHSO3 application caused an increase in the ATP content in leaves. Spraying PMS (a cofactor catalysing the photophosphorylation cycle) and NaHSO3 separately or together on leaves resulted in an increase in photosynthesis with all treatments. There was no additional effect on photosynthetic rate when the mixture was applied, suggesting that the mechanism by which NaHSO3 promotes photosynthesis is similar to the mechanism by which PMS acts and that both of compounds enhanced the supply of ATP. After spraying a solution of NaHSO3 on leaves, compared with the WT Kitaake rice, a greater enhancement of net photosynthetic rate was observed in PEPC transgenic (PC) and PEPC+PPDK transgenic (PC+PK) rice, with the greatest increase being observed in the latter group. Therefore ATP supply may become the limiting factor that concentrates CO2 in rice leaves transformed with an exogenous PEPC gene and exogenous PEPC+PPDK genes.展开更多
文摘In this study,we investigated the optimal concentration of nutrient-mist and NaHSO3 for cutting propagation of Juniperus squamata ’Blue Star’,as well as the variation in the contents of chlorophyll in leaves,and solubility carbohydrate,soluble protein and other nutrition in leaves and bases of cuttings during rooting.Cuttings of grade Ⅲ(the lenghth of cutting bases lignified from 0.1 to 0.2 cm) was treated by 1/4MS+NaHSO3(200 mg·L-1),and the rooting rate rose 35% and root qualities were improved compared with control experiment,for there were different effects among three grade cuttings.The treatment of grade Ⅲ cuttings also resulted in higher content of soluble sugar and starch of those bases during rooting,especially obviously different for fifteen days,but less effect on the chlorophyll and some nutrition content of cutting leaves.
基金国家自然科学基金，the National Key Project of the International Cooperation for Science and Technology
文摘: Spraying a 1–2 mmol/L solution of NaHSO3 on the leaves of wild-type rice (Oryza sativa L.) Kitaake (WT), phosphoenolpyruvate carboxylase (PEPC) transgenic (PC) rice and PEPC+phosphate dikinase (PPDK) transgenic rice (PC+PK), in which the germplasm was transformed with wild-type Kitaake as the gene receptor, resulted in an enhancement of the net photosynthetic rate by 23.0%, 28.8%, and 34.4%, respectively, for more than 3 d. It was also observed that NaHSO3 application caused an increase in the ATP content in leaves. Spraying PMS (a cofactor catalysing the photophosphorylation cycle) and NaHSO3 separately or together on leaves resulted in an increase in photosynthesis with all treatments. There was no additional effect on photosynthetic rate when the mixture was applied, suggesting that the mechanism by which NaHSO3 promotes photosynthesis is similar to the mechanism by which PMS acts and that both of compounds enhanced the supply of ATP. After spraying a solution of NaHSO3 on leaves, compared with the WT Kitaake rice, a greater enhancement of net photosynthetic rate was observed in PEPC transgenic (PC) and PEPC+PPDK transgenic (PC+PK) rice, with the greatest increase being observed in the latter group. Therefore ATP supply may become the limiting factor that concentrates CO2 in rice leaves transformed with an exogenous PEPC gene and exogenous PEPC+PPDK genes.
