A composite film of DNA/poly(3-methylthiophene)(P3MT) modified glassy carbon electrode(GCE) has been fabricated by electro-deposition method.P3MT film was first electropolymerized at the GCE and the DNA layer was then...A composite film of DNA/poly(3-methylthiophene)(P3MT) modified glassy carbon electrode(GCE) has been fabricated by electro-deposition method.P3MT film was first electropolymerized at the GCE and the DNA layer was then immobilized on the P3MT layer by electrochemical method.The voltammetric behavior of 8-hydroxy-2'-deoxyguanosine(8-OH-dG) at the composite film modified electrode was studied.The effects of scan rates,pH and the interference of uric acid(UA) on the voltammetric behavior and detection of 8-OH-dG were also discussed.The experimental results suggest that the electrochemical behavior of 8-OH-dG at the composite film modified electrode was greatly improved due to the combination of the advantages of P3MT and DNA.In 0.1 M pH 7.0 phosphate buffer solution(PBS),the anodic peak currents of 8-OH-dG were linear with the 8-OH-dG concentration in two intervals,viz.0.28―4.2 μM and 4.2―19.6 μM.The detection limit of 56 nM 8-OH-dG could be estimated(S/N=3).This proposed composite film modified electrode shows excellent reproducibility and stability.It may have the potential application for the detection of 8-OH-dG in human urine.展开更多
本文采用电化学性能独特的联吡啶钌（Ru（bpy）32＋）、氮掺杂石墨烯（NG）和Nafion膜构建了一种新型的盐酸异丙嗪电化学传感器。采用红外光谱和扫描电子显微镜对氮掺杂石墨烯的形貌进行了表征。在Nafion膜中添加导电性好、比表面积大...本文采用电化学性能独特的联吡啶钌（Ru（bpy）32＋）、氮掺杂石墨烯（NG）和Nafion膜构建了一种新型的盐酸异丙嗪电化学传感器。采用红外光谱和扫描电子显微镜对氮掺杂石墨烯的形貌进行了表征。在Nafion膜中添加导电性好、比表面积大的氮掺杂石墨烯可以增加电子传递速度并且可以防止联吡啶钌扩散到Nafion膜的非电活性区域而增加电极使用寿命。在p H 7.0的磷酸盐缓冲溶液中,盐酸异丙嗪在Ru（bpy）32＋/NG/Nafion修饰电极上的循环伏安曲线表明,与单一的裸玻碳电极、Ru（bpy）32＋/Nafion修饰电极以及NG/Nafion修饰电极相比,该修饰电极使盐酸异丙嗪得氧化峰电流显著增加,而峰电位明显负移,表明采用Ru（bpy）32＋/NG/Nafion膜制备的复合修饰电极对盐酸异丙嗪呈现出较强的电化学催化作用。优化实验条件后,发现在1.0×10^-6mol·L^-1~1.0×10^-4mol·L^-1.浓度范围内,盐酸异丙嗪的氧化峰电流与其浓度呈良好的线性关系,检测限为3.6×10^-7mol·L^-1。而且该电极的重现性、稳定性和选择性良好,采用标准加入法可成功用于商业盐酸异丙嗪注射液中盐酸异丙嗪的测定。展开更多
基金Supported by the National Natural Science Foundation of China (Grant Nos. 20475024 & 20775031)the Shandong Tai-Shan Scholar Research Fund
文摘A composite film of DNA/poly(3-methylthiophene)(P3MT) modified glassy carbon electrode(GCE) has been fabricated by electro-deposition method.P3MT film was first electropolymerized at the GCE and the DNA layer was then immobilized on the P3MT layer by electrochemical method.The voltammetric behavior of 8-hydroxy-2'-deoxyguanosine(8-OH-dG) at the composite film modified electrode was studied.The effects of scan rates,pH and the interference of uric acid(UA) on the voltammetric behavior and detection of 8-OH-dG were also discussed.The experimental results suggest that the electrochemical behavior of 8-OH-dG at the composite film modified electrode was greatly improved due to the combination of the advantages of P3MT and DNA.In 0.1 M pH 7.0 phosphate buffer solution(PBS),the anodic peak currents of 8-OH-dG were linear with the 8-OH-dG concentration in two intervals,viz.0.28―4.2 μM and 4.2―19.6 μM.The detection limit of 56 nM 8-OH-dG could be estimated(S/N=3).This proposed composite film modified electrode shows excellent reproducibility and stability.It may have the potential application for the detection of 8-OH-dG in human urine.
文摘本文采用电化学性能独特的联吡啶钌（Ru（bpy）32＋）、氮掺杂石墨烯（NG）和Nafion膜构建了一种新型的盐酸异丙嗪电化学传感器。采用红外光谱和扫描电子显微镜对氮掺杂石墨烯的形貌进行了表征。在Nafion膜中添加导电性好、比表面积大的氮掺杂石墨烯可以增加电子传递速度并且可以防止联吡啶钌扩散到Nafion膜的非电活性区域而增加电极使用寿命。在p H 7.0的磷酸盐缓冲溶液中,盐酸异丙嗪在Ru（bpy）32＋/NG/Nafion修饰电极上的循环伏安曲线表明,与单一的裸玻碳电极、Ru（bpy）32＋/Nafion修饰电极以及NG/Nafion修饰电极相比,该修饰电极使盐酸异丙嗪得氧化峰电流显著增加,而峰电位明显负移,表明采用Ru（bpy）32＋/NG/Nafion膜制备的复合修饰电极对盐酸异丙嗪呈现出较强的电化学催化作用。优化实验条件后,发现在1.0×10^-6mol·L^-1~1.0×10^-4mol·L^-1.浓度范围内,盐酸异丙嗪的氧化峰电流与其浓度呈良好的线性关系,检测限为3.6×10^-7mol·L^-1。而且该电极的重现性、稳定性和选择性良好,采用标准加入法可成功用于商业盐酸异丙嗪注射液中盐酸异丙嗪的测定。
