A kind of 9 nm gold nanoparticles was prepared with the trisodium citrate and used to label goat anti-human IgG to obtain an IgG immunoresonance scattering spectral probe. In pH 5.8 buffer solution and in the presence...A kind of 9 nm gold nanoparticles was prepared with the trisodium citrate and used to label goat anti-human IgG to obtain an IgG immunoresonance scattering spectral probe. In pH 5.8 buffer solution and in the presence of polyethylene glycol （PEG）, the immune reaction between gold-labeled goat anti-human IgG and IgG took place, and the resonance scattering intensity at 580 nm （I580nm） was enhanced greatly. The enhanced intensity AIRS is pro- portional to the IgG concentration from 1.3 to 1.5 X 10^3 ng.mL^-1, with a detection limit of 0.78 ng.mL ^-1. This assay showed high sensitivity and good selectivity for quantitative determination of IgG in human serum, with satisfactory results.展开更多
基金Project supported by the National Natural Science Foundation of China （Nos. 20667001, 20365001）, Natural Science Foundation of Guangxi （No. 2060213） and the Foundation of New Century Ten-Hundred-Thousand Talents of Guangxi.
文摘A kind of 9 nm gold nanoparticles was prepared with the trisodium citrate and used to label goat anti-human IgG to obtain an IgG immunoresonance scattering spectral probe. In pH 5.8 buffer solution and in the presence of polyethylene glycol （PEG）, the immune reaction between gold-labeled goat anti-human IgG and IgG took place, and the resonance scattering intensity at 580 nm （I580nm） was enhanced greatly. The enhanced intensity AIRS is pro- portional to the IgG concentration from 1.3 to 1.5 X 10^3 ng.mL^-1, with a detection limit of 0.78 ng.mL ^-1. This assay showed high sensitivity and good selectivity for quantitative determination of IgG in human serum, with satisfactory results.
