Objective: The aim of this study was to determine whether the presence of IL-18 polymorphisms -137 G/C and -607 A/C was associated with grade, clinical stage, and survival in patients with prostate cancer. Methods: Th...Objective: The aim of this study was to determine whether the presence of IL-18 polymorphisms -137 G/C and -607 A/C was associated with grade, clinical stage, and survival in patients with prostate cancer. Methods: The study cohort included 126 patients with prostate cancer. Control group consisted of 125 samples from Chinese population. Genomic DNA was extracted from EDTA-anticoagulated peripheral blood leukocytes by the salting-out method. The genotyping of the two IL-18 polymorphisms was performed using predesigned TaqMan SNP Genotyping Assays. Results: The studied IL-18 gene polymorphisms did not influence susceptibility to prostate cancer in the analyzed group of patients (IL-18-607, P = 0.342; IL-18-137 P = 0.715) but may contribute to disease onset and aggressiveness. IL-18-607 CC genotype was significantly associated with higher tumor grade (P = 0.025) and stage (P = 0.001). IL-18-137 GG genotype was correlated with higher tumor grade (P = 0.018) and stage (P = 0.007). The Cox proportional hazard model showed that tuumor grade and stage grouping were independent prognostic factors but IL-18 polymorphism was not. Polymorphism variants in the IL-18 gene (IL-18-607 and IL-18-137) may be associated with a worse prognosis for prostate cancer. Conclusion: High levels of IL-18 production may play a major role in the growth, invasion and metastasis of prostate cancer.展开更多
Compared with Renaissance music bound by religion and social system(Goldsbrough,2018,p.507),Viennese classical music has the characteristic of advocating rationality.This essay analyzes the early,middle,and late piano...Compared with Renaissance music bound by religion and social system(Goldsbrough,2018,p.507),Viennese classical music has the characteristic of advocating rationality.This essay analyzes the early,middle,and late piano sonatas by Haydn and Mozart,and takes the European Enlightenment as the historical background.Then it expounds the rational characteristics of Vienna classical music from the influences of European social economy and bourgeois new ideas on Vienna classical music in the 18th century.展开更多
Objective Recent studies have shown abnormal expression of octamer-binding transcription factor 4(OCT4) and interleukin-18(IL-18) to be related to cancer. However, the molecular mechanisms by which the IL-18 and OCT4 ...Objective Recent studies have shown abnormal expression of octamer-binding transcription factor 4(OCT4) and interleukin-18(IL-18) to be related to cancer. However, the molecular mechanisms by which the IL-18 and OCT4 gene polymorphisms are associated with prostate cancer remain unclear. In this study, we aimed to determine whether the presence of IL-18 and OCT4 polymorphisms were associated with size, grade, tumor, nodes and metastasis(TNM) stage, or survival in patients with prostate cancer.Methods Polymorphisms in OCT4 and IL-18 genes were evaluated to determine susceptibility to prostate cancer in 120 patients. A control group consisted of 125 Chinese participants. Genotyping was performed using TaqMan allelic discrimination assays, and statistical analysis was performed using SPSS. Results No association was found between OCT4 and IL-18 gene polymorphisms and prostate cancer susceptibility. For OCT4 AA and IL-18-607 CC genotypes, there was a significant association with higher tumor grade(P = 0.03 and P = 0.025) and stage(P = 0.04 and P = 0.001). The OCT4 and IL-18-137 GG genotype was correlated with higher tumor grade(P = 0.028) and stage(P = 0.008). Furthermore, OCT4 AA was significantly more frequent in patients with lymph node metastasis(P = 0.02) and distant metastasis(P = 0.01). The Cox proportional hazard model showed that tumor grade and stage grouping were independent prognostic factors but IL-18 and OCT4 polymorphisms were not. Conclusion The OCT4 gene may have a profound effect on prostate cancer risk. Polymorphism variants in the IL-18(IL-18-607 and IL-18-137) and OCT4 genes may be associated with poor prognoses for individuals with prostate cancer.展开更多
自新疆伊宁地区黄牛体表采集的盾糙璃眼蜱饥饿或半饱血成虫,带回实验室感染除脾牛。经血涂片检查发现,于感染后第21天,病牛血液中出现一种以圆环形与卵圆形为主的泰勒虫。经形态学观察和18 S rRNA基因测序和进化关系分析证明,它们为环...自新疆伊宁地区黄牛体表采集的盾糙璃眼蜱饥饿或半饱血成虫,带回实验室感染除脾牛。经血涂片检查发现,于感染后第21天,病牛血液中出现一种以圆环形与卵圆形为主的泰勒虫。经形态学观察和18 S rRNA基因测序和进化关系分析证明,它们为环形泰勒虫。将此饱血成蜱经实验室饲喂条件下,孵化出的幼虫分别饲喂于牛与家兔两种不同的动物,结果在饱血若虫阶段所有的蜱均从两种动物体表脱落,不再叮咬,饥饿成蜱又更换另一宿主,证明其是二宿主蜱。展开更多
文摘Objective: The aim of this study was to determine whether the presence of IL-18 polymorphisms -137 G/C and -607 A/C was associated with grade, clinical stage, and survival in patients with prostate cancer. Methods: The study cohort included 126 patients with prostate cancer. Control group consisted of 125 samples from Chinese population. Genomic DNA was extracted from EDTA-anticoagulated peripheral blood leukocytes by the salting-out method. The genotyping of the two IL-18 polymorphisms was performed using predesigned TaqMan SNP Genotyping Assays. Results: The studied IL-18 gene polymorphisms did not influence susceptibility to prostate cancer in the analyzed group of patients (IL-18-607, P = 0.342; IL-18-137 P = 0.715) but may contribute to disease onset and aggressiveness. IL-18-607 CC genotype was significantly associated with higher tumor grade (P = 0.025) and stage (P = 0.001). IL-18-137 GG genotype was correlated with higher tumor grade (P = 0.018) and stage (P = 0.007). The Cox proportional hazard model showed that tuumor grade and stage grouping were independent prognostic factors but IL-18 polymorphism was not. Polymorphism variants in the IL-18 gene (IL-18-607 and IL-18-137) may be associated with a worse prognosis for prostate cancer. Conclusion: High levels of IL-18 production may play a major role in the growth, invasion and metastasis of prostate cancer.
文摘Compared with Renaissance music bound by religion and social system(Goldsbrough,2018,p.507),Viennese classical music has the characteristic of advocating rationality.This essay analyzes the early,middle,and late piano sonatas by Haydn and Mozart,and takes the European Enlightenment as the historical background.Then it expounds the rational characteristics of Vienna classical music from the influences of European social economy and bourgeois new ideas on Vienna classical music in the 18th century.
基金Supported by grants from the China Postdoctoral Science Foundation(No.2014M139951)the Science and Technology Project of Nantong,Jiangsu Province(No.MS22016043)
文摘Objective Recent studies have shown abnormal expression of octamer-binding transcription factor 4(OCT4) and interleukin-18(IL-18) to be related to cancer. However, the molecular mechanisms by which the IL-18 and OCT4 gene polymorphisms are associated with prostate cancer remain unclear. In this study, we aimed to determine whether the presence of IL-18 and OCT4 polymorphisms were associated with size, grade, tumor, nodes and metastasis(TNM) stage, or survival in patients with prostate cancer.Methods Polymorphisms in OCT4 and IL-18 genes were evaluated to determine susceptibility to prostate cancer in 120 patients. A control group consisted of 125 Chinese participants. Genotyping was performed using TaqMan allelic discrimination assays, and statistical analysis was performed using SPSS. Results No association was found between OCT4 and IL-18 gene polymorphisms and prostate cancer susceptibility. For OCT4 AA and IL-18-607 CC genotypes, there was a significant association with higher tumor grade(P = 0.03 and P = 0.025) and stage(P = 0.04 and P = 0.001). The OCT4 and IL-18-137 GG genotype was correlated with higher tumor grade(P = 0.028) and stage(P = 0.008). Furthermore, OCT4 AA was significantly more frequent in patients with lymph node metastasis(P = 0.02) and distant metastasis(P = 0.01). The Cox proportional hazard model showed that tumor grade and stage grouping were independent prognostic factors but IL-18 and OCT4 polymorphisms were not. Conclusion The OCT4 gene may have a profound effect on prostate cancer risk. Polymorphism variants in the IL-18(IL-18-607 and IL-18-137) and OCT4 genes may be associated with poor prognoses for individuals with prostate cancer.
文摘自新疆伊宁地区黄牛体表采集的盾糙璃眼蜱饥饿或半饱血成虫,带回实验室感染除脾牛。经血涂片检查发现,于感染后第21天,病牛血液中出现一种以圆环形与卵圆形为主的泰勒虫。经形态学观察和18 S rRNA基因测序和进化关系分析证明,它们为环形泰勒虫。将此饱血成蜱经实验室饲喂条件下,孵化出的幼虫分别饲喂于牛与家兔两种不同的动物,结果在饱血若虫阶段所有的蜱均从两种动物体表脱落,不再叮咬,饥饿成蜱又更换另一宿主,证明其是二宿主蜱。
