In order to investigate the effects of lanthanum（Ⅲ） on cell ultrastructure of soybean leaves under elevated ultraviolet-B irradiation （UV-B, 280-320 nm）, the chloroplast ultrastructure of soybean seedlings was st...In order to investigate the effects of lanthanum（Ⅲ） on cell ultrastructure of soybean leaves under elevated ultraviolet-B irradiation （UV-B, 280-320 nm）, the chloroplast ultrastructure of soybean seedlings was studied by hydroponics under laboratory conditions. The resuits showed that the thylakoid in chloroplast was orderly and clearly as soybean leaves were pretreated by La（Ⅲ）. The thylakoid was indis- tinctly disordered, expanded and even indiscoverable in the chloroplast under UV-B stress. The impact on the thylakoid by the high intensity UV-B irradiation （T2） was bigger than that by the low intensity UV-B irradiation （T1）. However, the destruction of the chloroplast structure caused by UV-B stress was alleviated by La（Ⅲ）, and the arrangement of the thylakoid in the chloroplast became orderly and clearly. The effect of the alleviation by La（Ⅲ） under the low intensity UV-B irradiation （T1） was better than that under the high intensity UV-B irradiation （T2）.展开更多
Vitiligo is a common, acquired pigmentary disorder of unknown etiology with great impact on patient's appearance and quality of life. It presents a therapeutic challenge to many dermatologists. Photochemotherapy u...Vitiligo is a common, acquired pigmentary disorder of unknown etiology with great impact on patient's appearance and quality of life. It presents a therapeutic challenge to many dermatologists. Photochemotherapy using psoralen and ultraviolet A(UVA) therapy, topical and oral immunosuppresants, as well as cosmetic camouflage are also commonly employed with varying clinical efficacy. Phototherapy is a popular treatment option, which includes both of the generalized ultraviolet B(UVB) therapies, broadband UVB and narrowband UVB(NB-UVB). It has been used favorably, both alone as well as in combination with other agents like topical calcineurin inhibitors, vitamin-D analogs. Combination therapies are useful and may provide quicker regimentation and treat vitiligo with an additive mechanism of action than UVB phototherapy. Advances in technology may lead to the continuing use of UVB phototherapy as a treatment for vitiligo through the development of sophisticated devices and delivery systems as well as innovative application methods. These will provide increased therapeutic options for all vitiligo patients, particularly those with refractory disease. In this article, I have reviewed the available data pertaining to efficacy and safety issues for NB-UVB as monotherapy, its comparison with psoralen plus UVA and other modes of phototherapy, combination regimens that have been tried and future prospects of NB-UVB in vitiligo.展开更多
目的:观察紫外线B(UVB)照射HeLa细胞后IEX-1 mRNA表达的变化。方法:常规培养人宫颈癌HeLa细胞,采用RT-PCR法检测剂量为0、2、4、8、16、32 m J/cm2的UVB照射30 s后HeLa细胞中IEX-1 mRNA的表达情况,同法检测剂量为32 m J/cm2的UVB照射后...目的:观察紫外线B(UVB)照射HeLa细胞后IEX-1 mRNA表达的变化。方法:常规培养人宫颈癌HeLa细胞,采用RT-PCR法检测剂量为0、2、4、8、16、32 m J/cm2的UVB照射30 s后HeLa细胞中IEX-1 mRNA的表达情况,同法检测剂量为32 m J/cm2的UVB照射后即刻及培养1、2、4、8、16 h时IEX-1 mRNA的表达情况。结果:随UVB剂量的升高,HeLa细胞IEX-1 mRNA的表达量呈升高的趋势(F=644.725,P<0.001)。32 m J/cm2的UVB照射HeLa细胞后即刻及培养1、2、4、8、16 h,IEX-1 mRNA的表达呈升高的趋势(F=56.249,P<0.001),培养4 h后IEX-1 mRNA表达水平达峰值。结论:HeLa细胞中IEX-1 mRNA的表达可被UVB迅速、短暂地诱导,且具有剂量依赖性。展开更多
Objective:To study the changes of HSPs family expression in psoriasis lesions and their correlation with inflammatory response before and after narrow-band ultraviolet B (NB-UVB) treatment.Methods: Patients who were n...Objective:To study the changes of HSPs family expression in psoriasis lesions and their correlation with inflammatory response before and after narrow-band ultraviolet B (NB-UVB) treatment.Methods: Patients who were newly diagnosed with psoriasis vulgaris and treated in Dermatology Department of our hospital between March 2015 and May 2017 were selected and randomly divided into the NB-UVB group who received NB-UVB combined with conventional treatment and the control group who received conventional treatment. Before treatment and 20 d after treatment, proper amount of psoriasis lesion was collected to determine the expression of HSPs family molecules and lymphocyte transcription factors as well as the levels of inflammatory cytokines.Results: Compared with those before treatment, HSP27, HSP60, HSP90 , HSP90β, T-bet and Foxp3 mRNA expression as well as MCP-1, IFN-γ and IL-17 levels in psoriasis lesion of both groups significantly decreased whereas GATA-3 and RORγt mRNA expression as well as IL-10 and TGF-β1 levels significantly increased after treatment, and HSP27, HSP60, HSP90 , HSP90β, T-bet and Foxp3 mRNA expression as well as MCP-1, IFN-γ and IL-17 levels in psoriasis lesion of NB-UVB group were significantly lower than those of control group whereas GATA-3 and RORγt mRNA expression as well as IL-10 and TGF-β1 levels were significantly higher than those of control group;HSP27, HSP60, HSP90 and HSP90β mRNA expression in psoriasis lesion of NB-UVB group after treatment were positively correlated with MCP-1, IFN-γ and IL-17 levels as well as T-bet and Foxp3 mRNA expression, and negatively correlated with IL-10 and TGF-β1 levels as well as GATA-3 and RORγt mRNA expression.Conclusion: NB-UVB treatment of psoriasis can reduce the expression of HSPs family members and inhibit the inflammatory response mediated by HSPs.展开更多
基金supported by the National Natural Science Foundation of China (30570323)the Special Foundation for Rare Earth of State Planning Committee (IFZ20051210)
文摘In order to investigate the effects of lanthanum（Ⅲ） on cell ultrastructure of soybean leaves under elevated ultraviolet-B irradiation （UV-B, 280-320 nm）, the chloroplast ultrastructure of soybean seedlings was studied by hydroponics under laboratory conditions. The resuits showed that the thylakoid in chloroplast was orderly and clearly as soybean leaves were pretreated by La（Ⅲ）. The thylakoid was indis- tinctly disordered, expanded and even indiscoverable in the chloroplast under UV-B stress. The impact on the thylakoid by the high intensity UV-B irradiation （T2） was bigger than that by the low intensity UV-B irradiation （T1）. However, the destruction of the chloroplast structure caused by UV-B stress was alleviated by La（Ⅲ）, and the arrangement of the thylakoid in the chloroplast became orderly and clearly. The effect of the alleviation by La（Ⅲ） under the low intensity UV-B irradiation （T1） was better than that under the high intensity UV-B irradiation （T2）.
文摘Vitiligo is a common, acquired pigmentary disorder of unknown etiology with great impact on patient's appearance and quality of life. It presents a therapeutic challenge to many dermatologists. Photochemotherapy using psoralen and ultraviolet A(UVA) therapy, topical and oral immunosuppresants, as well as cosmetic camouflage are also commonly employed with varying clinical efficacy. Phototherapy is a popular treatment option, which includes both of the generalized ultraviolet B(UVB) therapies, broadband UVB and narrowband UVB(NB-UVB). It has been used favorably, both alone as well as in combination with other agents like topical calcineurin inhibitors, vitamin-D analogs. Combination therapies are useful and may provide quicker regimentation and treat vitiligo with an additive mechanism of action than UVB phototherapy. Advances in technology may lead to the continuing use of UVB phototherapy as a treatment for vitiligo through the development of sophisticated devices and delivery systems as well as innovative application methods. These will provide increased therapeutic options for all vitiligo patients, particularly those with refractory disease. In this article, I have reviewed the available data pertaining to efficacy and safety issues for NB-UVB as monotherapy, its comparison with psoralen plus UVA and other modes of phototherapy, combination regimens that have been tried and future prospects of NB-UVB in vitiligo.
文摘Objective:To study the changes of HSPs family expression in psoriasis lesions and their correlation with inflammatory response before and after narrow-band ultraviolet B (NB-UVB) treatment.Methods: Patients who were newly diagnosed with psoriasis vulgaris and treated in Dermatology Department of our hospital between March 2015 and May 2017 were selected and randomly divided into the NB-UVB group who received NB-UVB combined with conventional treatment and the control group who received conventional treatment. Before treatment and 20 d after treatment, proper amount of psoriasis lesion was collected to determine the expression of HSPs family molecules and lymphocyte transcription factors as well as the levels of inflammatory cytokines.Results: Compared with those before treatment, HSP27, HSP60, HSP90 , HSP90β, T-bet and Foxp3 mRNA expression as well as MCP-1, IFN-γ and IL-17 levels in psoriasis lesion of both groups significantly decreased whereas GATA-3 and RORγt mRNA expression as well as IL-10 and TGF-β1 levels significantly increased after treatment, and HSP27, HSP60, HSP90 , HSP90β, T-bet and Foxp3 mRNA expression as well as MCP-1, IFN-γ and IL-17 levels in psoriasis lesion of NB-UVB group were significantly lower than those of control group whereas GATA-3 and RORγt mRNA expression as well as IL-10 and TGF-β1 levels were significantly higher than those of control group;HSP27, HSP60, HSP90 and HSP90β mRNA expression in psoriasis lesion of NB-UVB group after treatment were positively correlated with MCP-1, IFN-γ and IL-17 levels as well as T-bet and Foxp3 mRNA expression, and negatively correlated with IL-10 and TGF-β1 levels as well as GATA-3 and RORγt mRNA expression.Conclusion: NB-UVB treatment of psoriasis can reduce the expression of HSPs family members and inhibit the inflammatory response mediated by HSPs.
