State Administration for Market Regulation and Standardization Administration of China have approved the following 374 national standards and 3 national standards with amendment.
State Administration for Market Regulation and Standardization Administration of China have approved the following 173 national standards and 3 national standards with amendment.
General Administration of Quality Supervision,Inspection and Quarantine of P.R.China and Standardization Administration of China have approved the following 163 national standards and 81 national reference materials.
General Administration of Quality Supervision, Inspection and Quarantine of P.R. China and Standardization Administration of China and Standardization Administration of China have approved the following 314 national s...General Administration of Quality Supervision, Inspection and Quarantine of P.R. China and Standardization Administration of China and Standardization Administration of China have approved the following 314 national standards.展开更多
General Administration of Quality Supervision, Inspection and Quarantine of P.R. China and Standardization Administration of China have approved the following 114 national standards and publicize now.……
General Administration of Quality Supervision,Inspection and Quarantine of P.R.China and Standardization Administration of China have approved the following 121 national standards and 34 reference materials publicize ...General Administration of Quality Supervision,Inspection and Quarantine of P.R.China and Standardization Administration of China have approved the following 121 national standards and 34 reference materials publicize now.展开更多
General Administration of Quality Supervision,Inspection and Quarantine of P.R. China and Standardization Administration of China have approved the following 3 national standards and publicize now.
目的:构建大鼠线粒体转录因子TFAM(A)、线粒体转录因子TFB1M(B1)、线粒体转录TFB2M(B2)及线粒体RNA聚合酶(POLRMT)的质粒标准品,为检测内耳细胞线粒体TFAM、TFB1M、TFB2M、POLRMT的mRNA表达水平做基础。方法:设计特异性引物和探针,提取...目的:构建大鼠线粒体转录因子TFAM(A)、线粒体转录因子TFB1M(B1)、线粒体转录TFB2M(B2)及线粒体RNA聚合酶(POLRMT)的质粒标准品,为检测内耳细胞线粒体TFAM、TFB1M、TFB2M、POLRMT的mRNA表达水平做基础。方法:设计特异性引物和探针,提取大鼠内耳组织总mRNA逆转录成c DNA,PCR扩增、纯化目的片段,将纯化产物与p Zero Back/blunt载体重组,提取重组质粒,经测序鉴定后,用实时荧光绝对定量PCR建立标准曲线。结果:测序结果与各目的序列一致,获得良好的标准曲线(R2>0.99)。结论:成功构建了各目的基因的质粒标准品。展开更多
文摘State Administration for Market Regulation and Standardization Administration of China have approved the following 374 national standards and 3 national standards with amendment.
文摘State Administration for Market Regulation and Standardization Administration of China have approved the following 173 national standards and 3 national standards with amendment.
文摘General Administration of Quality Supervision,Inspection and Quarantine of P.R.China and Standardization Administration of China have approved the following 163 national standards and 81 national reference materials.
文摘General Administration of Quality Supervision, Inspection and Quarantine of P.R. China and Standardization Administration of China and Standardization Administration of China have approved the following 314 national standards.
文摘 General Administration of Quality Supervision, Inspection and Quarantine of P.R. China and Standardization Administration of China have approved the following 114 national standards and publicize now.……
文摘General Administration of Quality Supervision,Inspection and Quarantine of P.R.China and Standardization Administration of China have approved the following 121 national standards and 34 reference materials publicize now.
文摘General Administration of Quality Supervision,Inspection and Quarantine of P.R. China and Standardization Administration of China have approved the following 3 national standards and publicize now.
文摘目的:构建大鼠线粒体转录因子TFAM(A)、线粒体转录因子TFB1M(B1)、线粒体转录TFB2M(B2)及线粒体RNA聚合酶(POLRMT)的质粒标准品,为检测内耳细胞线粒体TFAM、TFB1M、TFB2M、POLRMT的mRNA表达水平做基础。方法:设计特异性引物和探针,提取大鼠内耳组织总mRNA逆转录成c DNA,PCR扩增、纯化目的片段,将纯化产物与p Zero Back/blunt载体重组,提取重组质粒,经测序鉴定后,用实时荧光绝对定量PCR建立标准曲线。结果:测序结果与各目的序列一致,获得良好的标准曲线(R2>0.99)。结论:成功构建了各目的基因的质粒标准品。