[ Objective] The paper aimed to study the effects of Echinacea polysaccharide on secretion of IL-8mRNA by LPS-injured IEC-6 cells, in order to figure out the mechanism of EPS on injured cells. MethodI Total RNA was e...[ Objective] The paper aimed to study the effects of Echinacea polysaccharide on secretion of IL-8mRNA by LPS-injured IEC-6 cells, in order to figure out the mechanism of EPS on injured cells. MethodI Total RNA was extracted with TRlzon reagent. IL-8 mRNA was amplified by RT-PCR and detected by agar- ose gel electrophoresis. Furthermore, electrophoresis and image was analyzed. [ Result] The 50 μg/mL EPS could partially inhibit IL-8 mRNA level produced by -stimulated IEC-6; with the increasing concentration of EPS （200 and 500 μg/mL） , the inhibitory effect against IL-8 mRNA gradually enhanced. IEC-6 was pretreated by 50, 100,200 and 500 μg/mL EPS for ：24 h, then stimulated by 10 μg/ml 1,PS for 1 and 4 h, respectively. RT-PCR analysis showed that the expres- sion of 1L-8 mRNA induced by LPS was effectively inhibited by EPS; the inhibitory effect of EPS on expression of IL-8mRNA after stimulated by LPS for 4 h was more intense and the expression concentration was lower; the inhibitory rate at 4 h was higher than that at I h. [ Conclusion] EPS protected intestinal mucosa by inhibiting secretion of IL-8 mRNA by LPS-stimulated cells, and the inhibition of EPS was dependent to concentration and time.展开更多
基金Supported by National Natural Science Foundation of China(31472230)Natural Science Foundation of Hebei Province(C2014407068)Project of Hebei Department of Science and Technology(14966610D)
文摘[ Objective] The paper aimed to study the effects of Echinacea polysaccharide on secretion of IL-8mRNA by LPS-injured IEC-6 cells, in order to figure out the mechanism of EPS on injured cells. MethodI Total RNA was extracted with TRlzon reagent. IL-8 mRNA was amplified by RT-PCR and detected by agar- ose gel electrophoresis. Furthermore, electrophoresis and image was analyzed. [ Result] The 50 μg/mL EPS could partially inhibit IL-8 mRNA level produced by -stimulated IEC-6; with the increasing concentration of EPS （200 and 500 μg/mL） , the inhibitory effect against IL-8 mRNA gradually enhanced. IEC-6 was pretreated by 50, 100,200 and 500 μg/mL EPS for ：24 h, then stimulated by 10 μg/ml 1,PS for 1 and 4 h, respectively. RT-PCR analysis showed that the expres- sion of 1L-8 mRNA induced by LPS was effectively inhibited by EPS; the inhibitory effect of EPS on expression of IL-8mRNA after stimulated by LPS for 4 h was more intense and the expression concentration was lower; the inhibitory rate at 4 h was higher than that at I h. [ Conclusion] EPS protected intestinal mucosa by inhibiting secretion of IL-8 mRNA by LPS-stimulated cells, and the inhibition of EPS was dependent to concentration and time.
