Spatial linear features are often represented as a series of line segments joined by measured endpoints in surveying and geographic information science.There are not only the measuring errors of the endpoints but also...Spatial linear features are often represented as a series of line segments joined by measured endpoints in surveying and geographic information science.There are not only the measuring errors of the endpoints but also the modeling errors between the line segments and the actual geographical features.This paper presents a Brownian bridge error model for line segments combining both the modeling and measuring errors.First,the Brownian bridge is used to establish the position distribution of the actual geographic feature represented by the line segment.Second,an error propagation model with the constraints of the measuring error distribution of the endpoints is proposed.Third,a comprehensive error band of the line segment is constructed,wherein both the modeling and measuring errors are contained.The proposed error model can be used to evaluate line segments’overall accuracy and trustability influenced by modeling and measuring errors,and provides a comprehensive quality indicator for the geospatial data.展开更多
Electrical trees are an aging mechanismmost associated with partial discharge(PD)activities in crosslinked polyethylene(XLPE)insulation of high-voltage(HV)cables.Characterization of electrical tree structures gained c...Electrical trees are an aging mechanismmost associated with partial discharge(PD)activities in crosslinked polyethylene(XLPE)insulation of high-voltage(HV)cables.Characterization of electrical tree structures gained considerable attention from researchers since a deep understanding of the tree morphology is required to develop new insulation material.Two-dimensional(2D)optical microscopy is primarily used to examine tree structures and propagation shapes with image segmentation methods.However,since electrical trees can emerge in different shapes such as bush-type or branch-type,treeing images are complicated to segment due to manifestation of convoluted tree branches,leading to a high misclassification rate during segmentation.Therefore,this study proposed a new method for segmenting 2D electrical tree images based on the multi-scale line tracking algorithm(MSLTA)by integrating batch processing method.The proposed method,h-MSLTA aims to provide accurate segmentation of electrical tree images obtained over a period of tree propagation observation under optical microscopy.The initial phase involves XLPE sample preparation and treeing image acquisition under real-time microscopy observation.The treeing images are then sampled and binarized in pre-processing.In the next phase,segmentation of tree structures is performed using the h-MSLTA by utilizing batch processing in multiple instances of treeing duration.Finally,the comparative investigation has been conducted using standard performance assessment metrics,including accuracy,sensitivity,specificity,Dice coefficient and Matthew’s correlation coefficient(MCC).Based on segmentation performance evaluation against several established segmentation methods,h-MSLTA achieved better results of 95.43%accuracy,97.28%specificity,69.43%sensitivity rate with 23.38%and 24.16%average improvement in Dice coefficient and MCC score respectively over the original algorithm.In addition,h-MSLTA produced accurate measurement results of global tree parameters of length and width in comparison with the ground truth image.These results indicated that the proposed method had a solid performance in terms of segmenting electrical tree branches in 2D treeing images compared to other established techniques.展开更多
One hundred and fifty-eight microsatellite markers showing polymorphism among parents were used to survey the introgressed segments in the 50 near-isogenic lines of F_1 pollen sterility. Two hundred and sixty introgre...One hundred and fifty-eight microsatellite markers showing polymorphism among parents were used to survey the introgressed segments in the 50 near-isogenic lines of F_1 pollen sterility. Two hundred and sixty introgressed segments were detected in 50 near-isogenic lines, each carrying 5.2 introgressed segments on an average. Among the 260 segments, one hundred carrying F_1 pollen sterility loci concentrated on the region of F_1 pollen sterility genes, and the remaining one hundred and sixty without F_1 pollen sterility loci distributed randomly over 12 chromosomes. Both the average number and length of the introgressed segments decreased along with the increase of backcross generations. The number of introgressed segments was less than four and the length was less than 20 cM in the near-isogenic lines after backcrossing for four or more times.展开更多
Spatial data uncertainty can directly affect the quality of digital products and GIS-based decision making. On the basis of the characteristics of randomicity of positional data and fuzziness of attribute data, taking...Spatial data uncertainty can directly affect the quality of digital products and GIS-based decision making. On the basis of the characteristics of randomicity of positional data and fuzziness of attribute data, taking entropy as a measure, the stochastic entropy model of positional data uncertainty and fuzzy entropy model of attribute data uncertainty are proposed. As both randomic-ity and fuzziness usually simultaneously exist in linear segments, their omnibus effects are also investigated and quantified. A novel uncertainty measure, general entropy, is presented. The general entropy can be used as a uniform measure to quantify the total un-certainty caused by stochastic uncertainty and fuzzy uncertainty in GIS.展开更多
Grain weight, one of the major factors determining rice yield, is a typical quantitative trait control ed by multiple genes. With Guangluai 4 as recipient and Nipponbare as donor, a population of 119 chromosome single...Grain weight, one of the major factors determining rice yield, is a typical quantitative trait control ed by multiple genes. With Guangluai 4 as recipient and Nipponbare as donor, a population of 119 chromosome single segment substitution lines had been developed. Correlation analysis between grain weight and grain shape by SPSS revealed that 1 000-grain weight shared extremely significant posi-tive correlation with grain length and length-width ratio, but no significant correlation with grain width and thickness. The QTL analysis of grain weight was carried out using one-way analysis of variance and Dunnett's test. Nineteen stable QTLs re-sponsible for grain weight were identified over two years. Al 19 QTLs were identi-fied on al chromosomes except for chromosome 10 and 12 at a significance level of P≤0.001. Among them, 10 QTLs had a positive effect and were derived from the Nipponbare al ele, the additive effect of these QTLs ranged from 0.49 to 2.74 g, and the contributions of the additive effects ranged from 2.00% to 11.05%. Another 9 QTLs had a negative effect and were al derived from Guangluai 4 al ele, the ad-ditive effect of these QTLs ranged from 0.60 to 2.35 g, and the contributions of the additive effects ranged from 2.40% to 9.84%. The results provide a basis for the fine mapping and gene cloning of novel locus associated with rice grain weight.展开更多
In this study, a population of chromosome segment substitution lines (CSSLs) derived from the cross between 9311 (indica) and Nipponbare (japonica) was employed to map the quantitative trait loci (QTLs) for sa...In this study, a population of chromosome segment substitution lines (CSSLs) derived from the cross between 9311 (indica) and Nipponbare (japonica) was employed to map the quantitative trait loci (QTLs) for salt tolerance under the salt stress simulated with 0.5% NaCI, using survival rate as the index. The data were analyzed by QTL IciMapping v3.1, and the results showed that one QTL (QSsr3) related to salt tolerance was located in the vicinity of the marker RM1350 on chromosome 3, into a genetic interval of 113.2-132.8 cM, with a contribution rate of 17.75%. The additive effect was 10.9, indicating that the QTL derived from the parent Nipponbare improved the salt tolerance of rice at seedling stage. This study will provide a theoretical basis for the selection of salt tolerant rice germplasm.展开更多
In this study, a population of 119 chromosome segment substitution lines (CSSLs) derived from backcross between indica 9311 and japonica Nipponbare was employed to map quantitative trait loci (QTL) associated with...In this study, a population of 119 chromosome segment substitution lines (CSSLs) derived from backcross between indica 9311 and japonica Nipponbare was employed to map quantitative trait loci (QTL) associated with sheath blight resis-tance in rice with toothpick inoculation method. A total of three sheath blight resis-tance-associated QTLs (qsb8-1, qsb8-2 and qsb8-3) were identified, which were lo-cated on adjacent molecular markers RM3262, RM5485 and RM3496 of chromo-some 8; the genetic interval was 81.7cM-91.7cM, 91.7cM-108.1cM and 108.1cM-119.6cM, respectively. The additive effect of qsb8-2 was negative, indicating that sheath blight resistance of susceptible parent harboring qsb8-2 fragment was en-hanced; additive effects of qsb8-1 and qsb8-3 were positive, indicating that sheath blight resistance of susceptible parent harboring qsb8-1 and qsb8-3 fragments was reduced.展开更多
QTLs for plant height and its components on the substituted segments of fifty-two single segment substitution lines (SSSLs) in rice were identified through t-test (P〈0.001) for comparison between each SSSL and re...QTLs for plant height and its components on the substituted segments of fifty-two single segment substitution lines (SSSLs) in rice were identified through t-test (P〈0.001) for comparison between each SSSL and recipient parent Huajingxian 74. On the 14 substituted segments, 24 QTLs were detected, 10 for plant height, 2 for panicle length, 4 for length of the first internode from the top, 5 for length of the second internode from the top and 3 for length of the third internode from the top, respectively. All these QTLs were distributed on nine rice chromosomes except chromosomes 5, 9 and 11. The additive effect ranged from -4.08 to 3.98 cm, and the additive effect percentages varied from -19.35% to 10.43%.展开更多
Tiller is one of the most important agronomic traits which influences quantity and quality of effective panicles and finally influences yield in rice. It is important to understand "static" and "dynamic" informati...Tiller is one of the most important agronomic traits which influences quantity and quality of effective panicles and finally influences yield in rice. It is important to understand "static" and "dynamic" information of the QTLs for tillers in rice. This work was the first time to simultaneously map unconditional and conditional QTLs for tiller numbers at various stages by using single segment substitution lines in rice. Fourteen QTLs for tiller number, distributing on the corresponding substitution segments of chromosomes 1, 2, 3, 4, 6, 7 and 8 were detected. Both the number and the effect of the QTLs for tiller number were various at different stages, from 6 to 9 in the number and from 1.49 to 3.49 in the effect, respectively. Tiller number QTLs expressed in a time order, mainly detected at three stages of 0-7 d, 14-21 d and 35-42 d after transplanting with 6 positive, 9 random and 6 negative expressing QTLs, respectively. Each of the QTLs expressed one time at least during the whole duration of rice. The tiller number at a specific stage was determined by sum of QTL effects estimated by the unconditional method, while the increasing or decreasing number in a given time interval was controlled by the total of QTL effects estimated by the conditional method. These results demonstrated that it is highly effective and accurate for mapping of the QTLs by using single segment substitution lines and the conditional analysis methodology.展开更多
The microsatellite markers 484/485 and 484/W2R were used to identify the multiple alleles at the Wx locus in rice germplasm. Fifteen alleles were identified in 278 accessions by using microsatellite class and G-T poly...The microsatellite markers 484/485 and 484/W2R were used to identify the multiple alleles at the Wx locus in rice germplasm. Fifteen alleles were identified in 278 accessions by using microsatellite class and G-T polymorphism. Among these alleles, (CT)12-G, (CT)15-G, (CT)16-G, (CT)17-G, (CT)18-G and (CT)21-G have not been reported. Seventy-two single-segment substitution lines (SSSLs) carrying different alleles at the Wx locus were developed by using Huajingxian 74 with the (CT)11-G allele as a recipient and 20 accessions containing 12 different alleles at the Wx locus as donors. The estimated length of the substituted segments ranged from 2.2 to 77.3 cM with an average of 17.4 cM.展开更多
Length of grain affects the appearance, quality, and yield of rice. A rice long-grain chromosome segment substitution line Z744, with Nipponbare as the recipient parent and Xihui 18 as the donor parent, was identified...Length of grain affects the appearance, quality, and yield of rice. A rice long-grain chromosome segment substitution line Z744, with Nipponbare as the recipient parent and Xihui 18 as the donor parent, was identified. Z744 contains a total of six substitution segments distributed on chromosomes(Chrs.) 1, 2, 6, 7, and 12, with an average substitution length of 2.72 Mb. The grain length, ratio of length to width, and 1 000-grain weight of Z744 were significantly higher than those in Nipponbare. The plant height, panicle number, and seed-set ratio in Z744 were significantly lower than those in Nipponbare, but they were still 78.7 cm, 13.5 per plant, and 86.49%, respectively. Furthermore, eight QTLs of different traits were identified in the secondary F2 population, constructed by Nipponbare and Z744 hybridization. The grain weight of Z744 was controlled by two synergistic QTLs(qGWT1 and q GWT7) and two subtractive QTLs(qGWT2 and qGWT6), respectively. The increase in the grain weight of Z744 was caused mainly by the increase in grain length. Two QTLs were detected, qGL1 and qGL7-3, which accounted for 25.54 and 15.58% of phenotypic variation, respectively. A Chi-square test showed that the long-grain number and the short-grain number were in accordance with the 3:1 separation ratio, which indicates that the long grain is dominant over the short-grain and Z744 was controlled mainly by the principal effect qGL1. These results offered a good basis for further fine mapping of qGL1 and further dissection of other QTLs into single-segment substitution lines.展开更多
A chromosome segment substitution line (CSSL) is a powerful tool for combining quantitative trait locus (QTL) mapping with the pyramiding of desirable alleles. The rice CSSL Z1364 with increased kernel number was iden...A chromosome segment substitution line (CSSL) is a powerful tool for combining quantitative trait locus (QTL) mapping with the pyramiding of desirable alleles. The rice CSSL Z1364 with increased kernel number was identified in a BC3F8 population derived from a cross of Nipponbare as the recipient with Xihui 18 as the donor parent. Z1364 carried three substitution segments distributed on chromosomes 1, 6, and 8. The mean substitution length was 1.19 Mb. Of 17 QTL identified on the substitution segments, qSP1 for spikelets per panicle, qSSD1 for seed-set density, and qNSB1 for number of secondary branches explained respectively 57.34%, 87.7%, and 49.44% of the corresponding phenotypic variance and were all linked to RM6777. Chi-square analysis showed that the increased kernel number in Z1364 was inherited recessively by a single gene. By fine mapping, qSP1 was delimited to a 50-kb region on the short arm of chromosome 1. Based on DNA sequence, a previously uncharacterized rice homolog of Arabidopsis thaliana AT4G32551 was identified as a candidate gene for qSP1 in which mutation increases the number of spikelets and kernels in Z1364. qSP1 was expressed in all tissues, but particularly in 1-cm panicles. The expression levels of OsMADS22, GN1A, and DST were upregulated and those of LAX2, GNP1, and GHD7 were downregulated in Nipponbare. These results provide a foundation for functional research on qSP1.展开更多
Ab The cold tolerance at the bud bursting stage (CTB) was evaluated at 5℃ by using a set of 95 chromosome segment substitution lines (CSSLs) derived from an indica rice 9311 and a japonica rice Nipponbare with a ...Ab The cold tolerance at the bud bursting stage (CTB) was evaluated at 5℃ by using a set of 95 chromosome segment substitution lines (CSSLs) derived from an indica rice 9311 and a japonica rice Nipponbare with a genetic background of 9311. The result showed that six CSSLs had slightly stronger effect on CTB than 9311. Total four quantitative trait loci (QTLs) for CTB were preliminary mapped on chromosomes 5 and 7 by substitution mapping, qCTB-5-1, qCTB-5-2 and qCTB-5-3 were mapped in the region of RM267-RM1237, RM2422-RM6054 and RM3321-RM1054, which were 21.3 cM, 27.4 cM and 12.7 cM in genetic distance on rice chromosome 5, respectively, qCTB-7 was mapped in a 6.8-cM region of RM11-RM2752 on rice chromosome 7.展开更多
A backcross recombinant inbred line population consisting of 202 lines was developed from Xieqingzao B//Xieqingzao B / Dongxiang wild rice. The population was assayed with DNA markers and phenotyped on planthopper res...A backcross recombinant inbred line population consisting of 202 lines was developed from Xieqingzao B//Xieqingzao B / Dongxiang wild rice. The population was assayed with DNA markers and phenotyped on planthopper resistance and yield traits. A linkage map consisting of 119 DNA markers and spanned for 1188 cM over the 12 rice chromosomes was constructed. Thirty-two chromosomal segment substitution lines were selected based on the percentage of Xieqingzao B allele at marker loci. These lines are of great potential for gene mapping and alien gene introgression.展开更多
The rapid visco analyser (RVA) profile is an important factor for evaluation of the cooking and eating quality of rice. To improve rice quality, the identification of new quantitative trait loci (QTLs) for RVA pro...The rapid visco analyser (RVA) profile is an important factor for evaluation of the cooking and eating quality of rice. To improve rice quality, the identification of new quantitative trait loci (QTLs) for RVA profiling is of great significance. We used a japonica rice cultivar Nipponbare as the recipient and indica rice 9311 as the donor to develop a population containing 38 chromosome segment substitution lines (CSSLs) genotyped by a high-throughput re-sequencing strategy. In this study, the population and the parent lines, which contained similar apparent amylose contents, were used to map the QTLs of RVA properties including peak paste viscosity (PKV), hot paste viscosity (HPV), cool paste viscosity (CPV), breakdown viscosity (BKV), setback viscosity (SBV), consistency viscosity (CSV), peak time (PET) and pasting temperature (PAT). QTL analysis was carried out using one-way analysis of variance and Dunnett's test, and stable QTLs were identified over two years and under two environments. We identified 10 stable QTLs: qPKV2-1, qSBV2-1; qPKV5-1, qHPV5-1, qCPV5-1; qPKV7-1, qHPV7-1, qCPV7-1, qSBV7-1; and qPKV8-1 on chromosomes 2, 5, 7 and 8, respectively, with contributions ranging from -95.6% to 47.1%. Besides, there was pleiotropy in the QTLs on chromosomes 2, 5 and 7.展开更多
Rice grain shape is one of the important factors affecting grain quality and yield,but it is liable to be influenced by genetic backgrounds and environments.The chromosome single segment substitution lines(SSSLs) in r...Rice grain shape is one of the important factors affecting grain quality and yield,but it is liable to be influenced by genetic backgrounds and environments.The chromosome single segment substitution lines(SSSLs) in rice have been considered as ideal populations to identify the quantitative trait loci(QTLs).In this study,22 QTLs affecting rice grain shape were detected to be distributed on eight chromosomes except chromosomes 6,9,11 and 12 by using SSSLs.Among them,seven QTLs conditioned grain length,six conditioned grain width,five affected grain length-width ratio and four controlled grain thickness.展开更多
A closed form solution to the problem of segmenting multiple 3D motion models was proposed from straight-line optical flow. It introduced the multibody line optical flow constraint (MLOFC), a polynomial equation relat...A closed form solution to the problem of segmenting multiple 3D motion models was proposed from straight-line optical flow. It introduced the multibody line optical flow constraint (MLOFC), a polynomial equation relating motion models and line parameters. The motion models can be obtained analytically as the derivative of the MLOFC at the corresponding line measurement, without knowing the motion model associated with that line. Experiments on real and synthetic sequences were also presented.展开更多
Drought is a major abiotic constraint to rice production in rainfed lowland and insufficiently irrigated areas. The improvement of drought tolerant varieties is one of the strategies to reduce the negative effects of ...Drought is a major abiotic constraint to rice production in rainfed lowland and insufficiently irrigated areas. The improvement of drought tolerant varieties is one of the strategies to reduce the negative effects of drought. Quantitative trait loci (QTLs) for primary and secondary traits related to drought tolerance (DT) on chromosomes 1, 3, 4, 8 and 9 that determined from double haploid lines derived from a cross between CT9993 and IR62266 were introgressed and dissected into small pieces in the genetic background of Khao Dawk Mall 105 (KDML105) to develop chromosome segment substitution line (CSSL) population. The CSSLs were evaluated at the reproductive stage for their agronomic performance and yield components under drought stress, and results were compared with irrigated condition. The flowering of CSSL lines was 6 to 7 d earlier than KDML105. The mean values of grain yields in the CSSLs were higher than KDML105 under drought and irrigated conditions. At irrigated condition, the grain yields of introgression lines carrying DT-QTLs from chromosomes 4 and 8 were higher than that of KDML105, whereas other traits showed little difference with KDML105. Analysis indicated that grain yield has positive correlation with plant height, tiller and panicle number per plant, and total grain weight per plant under drought stress while negatively correlated with days to flowering. As mentioned above, CSSLs showing good adaptation under drought stress can be used as genetic materials to improve drought tolerance in Thai rainfed lowland rice breeding program, and as materials to dissect genes underlying drought tolerance.展开更多
基金National Natural Science Foundation of China(Nos.42071372,42221002)。
文摘Spatial linear features are often represented as a series of line segments joined by measured endpoints in surveying and geographic information science.There are not only the measuring errors of the endpoints but also the modeling errors between the line segments and the actual geographical features.This paper presents a Brownian bridge error model for line segments combining both the modeling and measuring errors.First,the Brownian bridge is used to establish the position distribution of the actual geographic feature represented by the line segment.Second,an error propagation model with the constraints of the measuring error distribution of the endpoints is proposed.Third,a comprehensive error band of the line segment is constructed,wherein both the modeling and measuring errors are contained.The proposed error model can be used to evaluate line segments’overall accuracy and trustability influenced by modeling and measuring errors,and provides a comprehensive quality indicator for the geospatial data.
基金the Ministry of Higher Education Malaysia for financially supported under the FundamentalResearch Grant Scheme (FRGS/1/2020/TK0/UNIMAP/02/17).
文摘Electrical trees are an aging mechanismmost associated with partial discharge(PD)activities in crosslinked polyethylene(XLPE)insulation of high-voltage(HV)cables.Characterization of electrical tree structures gained considerable attention from researchers since a deep understanding of the tree morphology is required to develop new insulation material.Two-dimensional(2D)optical microscopy is primarily used to examine tree structures and propagation shapes with image segmentation methods.However,since electrical trees can emerge in different shapes such as bush-type or branch-type,treeing images are complicated to segment due to manifestation of convoluted tree branches,leading to a high misclassification rate during segmentation.Therefore,this study proposed a new method for segmenting 2D electrical tree images based on the multi-scale line tracking algorithm(MSLTA)by integrating batch processing method.The proposed method,h-MSLTA aims to provide accurate segmentation of electrical tree images obtained over a period of tree propagation observation under optical microscopy.The initial phase involves XLPE sample preparation and treeing image acquisition under real-time microscopy observation.The treeing images are then sampled and binarized in pre-processing.In the next phase,segmentation of tree structures is performed using the h-MSLTA by utilizing batch processing in multiple instances of treeing duration.Finally,the comparative investigation has been conducted using standard performance assessment metrics,including accuracy,sensitivity,specificity,Dice coefficient and Matthew’s correlation coefficient(MCC).Based on segmentation performance evaluation against several established segmentation methods,h-MSLTA achieved better results of 95.43%accuracy,97.28%specificity,69.43%sensitivity rate with 23.38%and 24.16%average improvement in Dice coefficient and MCC score respectively over the original algorithm.In addition,h-MSLTA produced accurate measurement results of global tree parameters of length and width in comparison with the ground truth image.These results indicated that the proposed method had a solid performance in terms of segmenting electrical tree branches in 2D treeing images compared to other established techniques.
文摘One hundred and fifty-eight microsatellite markers showing polymorphism among parents were used to survey the introgressed segments in the 50 near-isogenic lines of F_1 pollen sterility. Two hundred and sixty introgressed segments were detected in 50 near-isogenic lines, each carrying 5.2 introgressed segments on an average. Among the 260 segments, one hundred carrying F_1 pollen sterility loci concentrated on the region of F_1 pollen sterility genes, and the remaining one hundred and sixty without F_1 pollen sterility loci distributed randomly over 12 chromosomes. Both the average number and length of the introgressed segments decreased along with the increase of backcross generations. The number of introgressed segments was less than four and the length was less than 20 cM in the near-isogenic lines after backcrossing for four or more times.
基金Supported by the Open Research Fund Program of Geomatics and Application Laboratory, Liaoning Technical University (No.2005004).
文摘Spatial data uncertainty can directly affect the quality of digital products and GIS-based decision making. On the basis of the characteristics of randomicity of positional data and fuzziness of attribute data, taking entropy as a measure, the stochastic entropy model of positional data uncertainty and fuzzy entropy model of attribute data uncertainty are proposed. As both randomic-ity and fuzziness usually simultaneously exist in linear segments, their omnibus effects are also investigated and quantified. A novel uncertainty measure, general entropy, is presented. The general entropy can be used as a uniform measure to quantify the total un-certainty caused by stochastic uncertainty and fuzzy uncertainty in GIS.
基金Supported by National Natural Science Foundation of China(31101131)National Key Technology Research and Development Program(2011BAD16B03)+1 种基金Agricultural Science Independent Innovation Foundation of Jiangsu Province[CX(12)1003]Key Technology Research and Development Program of Jiangsu Province(BE2012309)~~
文摘Grain weight, one of the major factors determining rice yield, is a typical quantitative trait control ed by multiple genes. With Guangluai 4 as recipient and Nipponbare as donor, a population of 119 chromosome single segment substitution lines had been developed. Correlation analysis between grain weight and grain shape by SPSS revealed that 1 000-grain weight shared extremely significant posi-tive correlation with grain length and length-width ratio, but no significant correlation with grain width and thickness. The QTL analysis of grain weight was carried out using one-way analysis of variance and Dunnett's test. Nineteen stable QTLs re-sponsible for grain weight were identified over two years. Al 19 QTLs were identi-fied on al chromosomes except for chromosome 10 and 12 at a significance level of P≤0.001. Among them, 10 QTLs had a positive effect and were derived from the Nipponbare al ele, the additive effect of these QTLs ranged from 0.49 to 2.74 g, and the contributions of the additive effects ranged from 2.00% to 11.05%. Another 9 QTLs had a negative effect and were al derived from Guangluai 4 al ele, the ad-ditive effect of these QTLs ranged from 0.60 to 2.35 g, and the contributions of the additive effects ranged from 2.40% to 9.84%. The results provide a basis for the fine mapping and gene cloning of novel locus associated with rice grain weight.
文摘In this study, a population of chromosome segment substitution lines (CSSLs) derived from the cross between 9311 (indica) and Nipponbare (japonica) was employed to map the quantitative trait loci (QTLs) for salt tolerance under the salt stress simulated with 0.5% NaCI, using survival rate as the index. The data were analyzed by QTL IciMapping v3.1, and the results showed that one QTL (QSsr3) related to salt tolerance was located in the vicinity of the marker RM1350 on chromosome 3, into a genetic interval of 113.2-132.8 cM, with a contribution rate of 17.75%. The additive effect was 10.9, indicating that the QTL derived from the parent Nipponbare improved the salt tolerance of rice at seedling stage. This study will provide a theoretical basis for the selection of salt tolerant rice germplasm.
基金Supported by Specific Fund for the Independent Innovation of Agricultural Science and Technology[CX(11)1020]~~
文摘In this study, a population of 119 chromosome segment substitution lines (CSSLs) derived from backcross between indica 9311 and japonica Nipponbare was employed to map quantitative trait loci (QTL) associated with sheath blight resis-tance in rice with toothpick inoculation method. A total of three sheath blight resis-tance-associated QTLs (qsb8-1, qsb8-2 and qsb8-3) were identified, which were lo-cated on adjacent molecular markers RM3262, RM5485 and RM3496 of chromo-some 8; the genetic interval was 81.7cM-91.7cM, 91.7cM-108.1cM and 108.1cM-119.6cM, respectively. The additive effect of qsb8-2 was negative, indicating that sheath blight resistance of susceptible parent harboring qsb8-2 fragment was en-hanced; additive effects of qsb8-1 and qsb8-3 were positive, indicating that sheath blight resistance of susceptible parent harboring qsb8-1 and qsb8-3 fragments was reduced.
基金the key project ofNational Natural Science Foundation of China(30330370) the team project of Natural ScienceFoundation of Guangdong Province (20003023).
文摘QTLs for plant height and its components on the substituted segments of fifty-two single segment substitution lines (SSSLs) in rice were identified through t-test (P〈0.001) for comparison between each SSSL and recipient parent Huajingxian 74. On the 14 substituted segments, 24 QTLs were detected, 10 for plant height, 2 for panicle length, 4 for length of the first internode from the top, 5 for length of the second internode from the top and 3 for length of the third internode from the top, respectively. All these QTLs were distributed on nine rice chromosomes except chromosomes 5, 9 and 11. The additive effect ranged from -4.08 to 3.98 cm, and the additive effect percentages varied from -19.35% to 10.43%.
基金supported by the grants from the National.Basic Research Program of China(2006CB 101700)the National Natural Science Foundation of China(30330370).
文摘Tiller is one of the most important agronomic traits which influences quantity and quality of effective panicles and finally influences yield in rice. It is important to understand "static" and "dynamic" information of the QTLs for tillers in rice. This work was the first time to simultaneously map unconditional and conditional QTLs for tiller numbers at various stages by using single segment substitution lines in rice. Fourteen QTLs for tiller number, distributing on the corresponding substitution segments of chromosomes 1, 2, 3, 4, 6, 7 and 8 were detected. Both the number and the effect of the QTLs for tiller number were various at different stages, from 6 to 9 in the number and from 1.49 to 3.49 in the effect, respectively. Tiller number QTLs expressed in a time order, mainly detected at three stages of 0-7 d, 14-21 d and 35-42 d after transplanting with 6 positive, 9 random and 6 negative expressing QTLs, respectively. Each of the QTLs expressed one time at least during the whole duration of rice. The tiller number at a specific stage was determined by sum of QTL effects estimated by the unconditional method, while the increasing or decreasing number in a given time interval was controlled by the total of QTL effects estimated by the conditional method. These results demonstrated that it is highly effective and accurate for mapping of the QTLs by using single segment substitution lines and the conditional analysis methodology.
基金supported by the key project of National Natural Science Foundation of China(30330370).
文摘The microsatellite markers 484/485 and 484/W2R were used to identify the multiple alleles at the Wx locus in rice germplasm. Fifteen alleles were identified in 278 accessions by using microsatellite class and G-T polymorphism. Among these alleles, (CT)12-G, (CT)15-G, (CT)16-G, (CT)17-G, (CT)18-G and (CT)21-G have not been reported. Seventy-two single-segment substitution lines (SSSLs) carrying different alleles at the Wx locus were developed by using Huajingxian 74 with the (CT)11-G allele as a recipient and 20 accessions containing 12 different alleles at the Wx locus as donors. The estimated length of the substituted segments ranged from 2.2 to 77.3 cM with an average of 17.4 cM.
基金supported by the National Natural Science Foundation of China(31871593)the Chongqing Science and Technology Commission Special Project,China(cstc2016shms-ztzx0032)the Southwest University Innovation Team Project,China(XDJK2017A004)。
文摘Length of grain affects the appearance, quality, and yield of rice. A rice long-grain chromosome segment substitution line Z744, with Nipponbare as the recipient parent and Xihui 18 as the donor parent, was identified. Z744 contains a total of six substitution segments distributed on chromosomes(Chrs.) 1, 2, 6, 7, and 12, with an average substitution length of 2.72 Mb. The grain length, ratio of length to width, and 1 000-grain weight of Z744 were significantly higher than those in Nipponbare. The plant height, panicle number, and seed-set ratio in Z744 were significantly lower than those in Nipponbare, but they were still 78.7 cm, 13.5 per plant, and 86.49%, respectively. Furthermore, eight QTLs of different traits were identified in the secondary F2 population, constructed by Nipponbare and Z744 hybridization. The grain weight of Z744 was controlled by two synergistic QTLs(qGWT1 and q GWT7) and two subtractive QTLs(qGWT2 and qGWT6), respectively. The increase in the grain weight of Z744 was caused mainly by the increase in grain length. Two QTLs were detected, qGL1 and qGL7-3, which accounted for 25.54 and 15.58% of phenotypic variation, respectively. A Chi-square test showed that the long-grain number and the short-grain number were in accordance with the 3:1 separation ratio, which indicates that the long grain is dominant over the short-grain and Z744 was controlled mainly by the principal effect qGL1. These results offered a good basis for further fine mapping of qGL1 and further dissection of other QTLs into single-segment substitution lines.
基金supported by the National Key Research Plan Project (2017YFD0101107)the Chongqing Science and Technology Commission Special Project (cstc2016shmsztzx0032)the Southwest University Innovation Team Project (XDJK2017A004)
文摘A chromosome segment substitution line (CSSL) is a powerful tool for combining quantitative trait locus (QTL) mapping with the pyramiding of desirable alleles. The rice CSSL Z1364 with increased kernel number was identified in a BC3F8 population derived from a cross of Nipponbare as the recipient with Xihui 18 as the donor parent. Z1364 carried three substitution segments distributed on chromosomes 1, 6, and 8. The mean substitution length was 1.19 Mb. Of 17 QTL identified on the substitution segments, qSP1 for spikelets per panicle, qSSD1 for seed-set density, and qNSB1 for number of secondary branches explained respectively 57.34%, 87.7%, and 49.44% of the corresponding phenotypic variance and were all linked to RM6777. Chi-square analysis showed that the increased kernel number in Z1364 was inherited recessively by a single gene. By fine mapping, qSP1 was delimited to a 50-kb region on the short arm of chromosome 1. Based on DNA sequence, a previously uncharacterized rice homolog of Arabidopsis thaliana AT4G32551 was identified as a candidate gene for qSP1 in which mutation increases the number of spikelets and kernels in Z1364. qSP1 was expressed in all tissues, but particularly in 1-cm panicles. The expression levels of OsMADS22, GN1A, and DST were upregulated and those of LAX2, GNP1, and GHD7 were downregulated in Nipponbare. These results provide a foundation for functional research on qSP1.
基金supported by the Special Program for Rice Scientific Research in Ministry of Agriculture, China (Grant No.nyhyzx 07-001-006)Special Funds for Construction of Modern Agricultural Industry R & D SystemSelf-directed Innovation Fund of Agricultural Science and Technology in Jiangsu Province, China (Grant No. CX [09] 634)
文摘Ab The cold tolerance at the bud bursting stage (CTB) was evaluated at 5℃ by using a set of 95 chromosome segment substitution lines (CSSLs) derived from an indica rice 9311 and a japonica rice Nipponbare with a genetic background of 9311. The result showed that six CSSLs had slightly stronger effect on CTB than 9311. Total four quantitative trait loci (QTLs) for CTB were preliminary mapped on chromosomes 5 and 7 by substitution mapping, qCTB-5-1, qCTB-5-2 and qCTB-5-3 were mapped in the region of RM267-RM1237, RM2422-RM6054 and RM3321-RM1054, which were 21.3 cM, 27.4 cM and 12.7 cM in genetic distance on rice chromosome 5, respectively, qCTB-7 was mapped in a 6.8-cM region of RM11-RM2752 on rice chromosome 7.
文摘A backcross recombinant inbred line population consisting of 202 lines was developed from Xieqingzao B//Xieqingzao B / Dongxiang wild rice. The population was assayed with DNA markers and phenotyped on planthopper resistance and yield traits. A linkage map consisting of 119 DNA markers and spanned for 1188 cM over the 12 rice chromosomes was constructed. Thirty-two chromosomal segment substitution lines were selected based on the percentage of Xieqingzao B allele at marker loci. These lines are of great potential for gene mapping and alien gene introgression.
基金supported by grants from the National Natural Science Foundation of China (Grant No. 31071383)the Ministry of Science and Technology of China (Grant Nos. 2012AA10A302-7 and 2013ZX080 09003-004)the Jiangsu Government of China (Grant Nos. BK2012010 and CX(12)1003)
文摘The rapid visco analyser (RVA) profile is an important factor for evaluation of the cooking and eating quality of rice. To improve rice quality, the identification of new quantitative trait loci (QTLs) for RVA profiling is of great significance. We used a japonica rice cultivar Nipponbare as the recipient and indica rice 9311 as the donor to develop a population containing 38 chromosome segment substitution lines (CSSLs) genotyped by a high-throughput re-sequencing strategy. In this study, the population and the parent lines, which contained similar apparent amylose contents, were used to map the QTLs of RVA properties including peak paste viscosity (PKV), hot paste viscosity (HPV), cool paste viscosity (CPV), breakdown viscosity (BKV), setback viscosity (SBV), consistency viscosity (CSV), peak time (PET) and pasting temperature (PAT). QTL analysis was carried out using one-way analysis of variance and Dunnett's test, and stable QTLs were identified over two years and under two environments. We identified 10 stable QTLs: qPKV2-1, qSBV2-1; qPKV5-1, qHPV5-1, qCPV5-1; qPKV7-1, qHPV7-1, qCPV7-1, qSBV7-1; and qPKV8-1 on chromosomes 2, 5, 7 and 8, respectively, with contributions ranging from -95.6% to 47.1%. Besides, there was pleiotropy in the QTLs on chromosomes 2, 5 and 7.
基金supported by the National Basic Research Program of China(Grant No.2005CB120807)
文摘Rice grain shape is one of the important factors affecting grain quality and yield,but it is liable to be influenced by genetic backgrounds and environments.The chromosome single segment substitution lines(SSSLs) in rice have been considered as ideal populations to identify the quantitative trait loci(QTLs).In this study,22 QTLs affecting rice grain shape were detected to be distributed on eight chromosomes except chromosomes 6,9,11 and 12 by using SSSLs.Among them,seven QTLs conditioned grain length,six conditioned grain width,five affected grain length-width ratio and four controlled grain thickness.
基金The National Natural Science Foundation of China (No. 60675017) The National Basic Research Program (973) of China (No. 2006CB303103)
文摘A closed form solution to the problem of segmenting multiple 3D motion models was proposed from straight-line optical flow. It introduced the multibody line optical flow constraint (MLOFC), a polynomial equation relating motion models and line parameters. The motion models can be obtained analytically as the derivative of the MLOFC at the corresponding line measurement, without knowing the motion model associated with that line. Experiments on real and synthetic sequences were also presented.
基金supported by the National Centerfor Genetic Engineering and Biotechnology(BIOTEC),Thailandpartially funded by the Center of Excellence on Agricultural Biotechnology,Science and Technology Postgraduate Education and Research Development Office,Office of Higher Education Commission,Ministry of Education (AG-BIO/PERDO-CHE),Thailand
文摘Drought is a major abiotic constraint to rice production in rainfed lowland and insufficiently irrigated areas. The improvement of drought tolerant varieties is one of the strategies to reduce the negative effects of drought. Quantitative trait loci (QTLs) for primary and secondary traits related to drought tolerance (DT) on chromosomes 1, 3, 4, 8 and 9 that determined from double haploid lines derived from a cross between CT9993 and IR62266 were introgressed and dissected into small pieces in the genetic background of Khao Dawk Mall 105 (KDML105) to develop chromosome segment substitution line (CSSL) population. The CSSLs were evaluated at the reproductive stage for their agronomic performance and yield components under drought stress, and results were compared with irrigated condition. The flowering of CSSL lines was 6 to 7 d earlier than KDML105. The mean values of grain yields in the CSSLs were higher than KDML105 under drought and irrigated conditions. At irrigated condition, the grain yields of introgression lines carrying DT-QTLs from chromosomes 4 and 8 were higher than that of KDML105, whereas other traits showed little difference with KDML105. Analysis indicated that grain yield has positive correlation with plant height, tiller and panicle number per plant, and total grain weight per plant under drought stress while negatively correlated with days to flowering. As mentioned above, CSSLs showing good adaptation under drought stress can be used as genetic materials to improve drought tolerance in Thai rainfed lowland rice breeding program, and as materials to dissect genes underlying drought tolerance.