The molecular variation of satellite DNA associated with Tobacco curly shoot virus (TbCSV) was compared by DNA sequencing of progenies of TbCSV DNA in Nicotiana benthamiana and N. glutinosa plants agroinoculated with ...The molecular variation of satellite DNA associated with Tobacco curly shoot virus (TbCSV) was compared by DNA sequencing of progenies of TbCSV DNA in Nicotiana benthamiana and N. glutinosa plants agroinoculated with infectious clones of TbCSV and its associated DNA. Comparisons indicated that TbCSV DNA populations had the higher levels of mutation frequency in N. glutinosa than that in N. benthamiana, and mutations generated in N. benthamiana were found upstream or downstream of C1 ORF whereas those in N. glutinosa were mostly located in A-rich region. No mutation was found in C1 ORF and satellite conserved region of DNA. The biological significance of the mutation accumulation and its role in disease epidemic were discussed.展开更多
Plant growth, gas exchange characteristics, chlorophyll fluorescence, disease index, and disease prevention efficiency of LaCl3 in tobacco （Nicotiana glutinosa） plants infected by Fny-CMV （fny stain of cucumber mos...Plant growth, gas exchange characteristics, chlorophyll fluorescence, disease index, and disease prevention efficiency of LaCl3 in tobacco （Nicotiana glutinosa） plants infected by Fny-CMV （fny stain of cucumber mosaic virus） strain were determined. Leaf area, chloro-phyll and carotenoid contents, maximum photosynthesis rate, apparent quantum yield, and carboxylation efficiency dramatically decreased after 5 weeks post inoculation. The plants infected by Fny-CMV only presented much severer symptom than those infected in the presence of appropriate concentration of LaCl3. ETR （apparent rate of photosynthetic electron transport）, NPQ （nonphotochemical quenching）, qP （coef-ficient of photochemical quenching）, and yield （II） in infected tobacco plants obviously reduced in higher light intensity after 5 weeks post inoculation. And these fluorescence parameters in Fny-infected plants obviously reduced compared with those in the plants infected by Fny in the presence of LaCl3. Together with the growth status and disease index, it revealed that exogenously appropriate concentration of LaCl3 could significantly alleviate the damage of tobacco seedlings caused by Fny-CMV.展开更多
文摘The molecular variation of satellite DNA associated with Tobacco curly shoot virus (TbCSV) was compared by DNA sequencing of progenies of TbCSV DNA in Nicotiana benthamiana and N. glutinosa plants agroinoculated with infectious clones of TbCSV and its associated DNA. Comparisons indicated that TbCSV DNA populations had the higher levels of mutation frequency in N. glutinosa than that in N. benthamiana, and mutations generated in N. benthamiana were found upstream or downstream of C1 ORF whereas those in N. glutinosa were mostly located in A-rich region. No mutation was found in C1 ORF and satellite conserved region of DNA. The biological significance of the mutation accumulation and its role in disease epidemic were discussed.
基金Project supported by National Natural Science Foundation of China (30670482)
文摘Plant growth, gas exchange characteristics, chlorophyll fluorescence, disease index, and disease prevention efficiency of LaCl3 in tobacco （Nicotiana glutinosa） plants infected by Fny-CMV （fny stain of cucumber mosaic virus） strain were determined. Leaf area, chloro-phyll and carotenoid contents, maximum photosynthesis rate, apparent quantum yield, and carboxylation efficiency dramatically decreased after 5 weeks post inoculation. The plants infected by Fny-CMV only presented much severer symptom than those infected in the presence of appropriate concentration of LaCl3. ETR （apparent rate of photosynthetic electron transport）, NPQ （nonphotochemical quenching）, qP （coef-ficient of photochemical quenching）, and yield （II） in infected tobacco plants obviously reduced in higher light intensity after 5 weeks post inoculation. And these fluorescence parameters in Fny-infected plants obviously reduced compared with those in the plants infected by Fny in the presence of LaCl3. Together with the growth status and disease index, it revealed that exogenously appropriate concentration of LaCl3 could significantly alleviate the damage of tobacco seedlings caused by Fny-CMV.
