In the mitochondria and chloroplasts of flowering plants (angiosperms), transcripts of protein-coding genes are altered after synthesis so that their final primary nucleotide sequence differs from that of the correspo...In the mitochondria and chloroplasts of flowering plants (angiosperms), transcripts of protein-coding genes are altered after synthesis so that their final primary nucleotide sequence differs from that of the corresponding DNA sequence. This posttranscriptional mRNA editing consists almost exclusively of C-to-U substitutions (direct) and less frequently of U-to-C substitution (reverse). Editing occurs predominantly within coding regions, mostly at isolated C residues, and usually at first or second positions of codons, thereby almost always changing the amino acid from that specified by the unedited codon. Editing may also create initiation and termination codons. The effect of C-to-U RNA editing in plants is to make proteins encoded by plant organelles more similar in sequence to their non plant homologs, then specific C-to- U editing events are essential for the production of functional plant mitochondrial proteins. Our attention has been devoted to the study of the mRNA editing in cox3 mitochondrial gene of fern Asplenium nidus. This study reveals the extreme importance of both C-to-U and U-to-C substitutions for protein expression.展开更多
Asplenium nidus L. commonly called as Bird’s Nest Fern, is a threatened, ornamental fern, which is widely used as novel foliage ornamental plant and local people use it in worship. The taxon is threatened due to over...Asplenium nidus L. commonly called as Bird’s Nest Fern, is a threatened, ornamental fern, which is widely used as novel foliage ornamental plant and local people use it in worship. The taxon is threatened due to over exploitation, habitat destruction and genetic barriers. To understand the constraints in the regeneration, reproductive biology studies are made. It is observed that more sporophytes are produced in composite population (13.3%) in comparison to isolate population (10%). This pattern is suggestive of the fact that the parental sporophyte is heterozygous for recessive sporophytic lethal. On the basis of the results obtained A. nidus was initially adapted for outbreeding with the capacity for considerable amount of inbreeding. The low potential of sporophyte production in isolate gametophyte could be the constitution of the zygotic genotype.展开更多
[Objective] The paper was to explore the oviposition site selection of Anoplophora glabripennis in sugar maple and its gnawing nidus oviposition habits. [Method] Through field and indoor rearing observation, the ovipo...[Objective] The paper was to explore the oviposition site selection of Anoplophora glabripennis in sugar maple and its gnawing nidus oviposition habits. [Method] Through field and indoor rearing observation, the oviposition site selection of A. glabripennis in sugar maple was studied, and the gnawing nidus oviposition habits were analyzed. [Result] The height of oviposition site of A. glabripennis in sugar maple, the number observation holes （gnawing nidus oviposition points） and bark thickness in oviposition site showed linear correlation with diameter of branch at breast height. The gnawing nidus oviposition habit of A. glabripennis in sugar maple was mainly divided into 4 stages： gnawing nidus site selection, gnawing nidus, oviposition and nidus sealing, the duration from gnawing nidus to nidus sealing was（38.29±16.47）min. [Conclusion] The study provided reference for comprehensive prevention of A. glabripennis in greening tree species sugar maple, as well as further study on reproductive behavior and regulation mechanism of A. glabripennis.展开更多
目的 建立一种Qu ECh ERS EMR-Lipid净化结合同位素稀释-超高效液相色谱-串联质谱技术(UPLC-MS/MS)同时测定蜂房及其制剂中10种真菌毒素的检测方法。方法 样品经80%乙腈水溶液提取,Qu ECh ERS EMRLipid净化,采用UPLC-MS/MS,在多反应监测...目的 建立一种Qu ECh ERS EMR-Lipid净化结合同位素稀释-超高效液相色谱-串联质谱技术(UPLC-MS/MS)同时测定蜂房及其制剂中10种真菌毒素的检测方法。方法 样品经80%乙腈水溶液提取,Qu ECh ERS EMRLipid净化,采用UPLC-MS/MS,在多反应监测(MRM)模式下进行测定,内标法定量。结果 10种真菌毒素含量在各自质量浓度范围内具有较好的线性关系(r>0.999),目标化合物在低、中、高3个质量浓度下的平均回收率为83.8%~107.1%(RSD<6.5%),定量限(LOQ)为0.18~129μg/kg。结论 该法前处理步骤简便,净化效果良好,提高了样品检测效率,内标法定量精准可靠,适用于蜂房及其制剂中10种真菌毒素的同时检测。展开更多
文摘In the mitochondria and chloroplasts of flowering plants (angiosperms), transcripts of protein-coding genes are altered after synthesis so that their final primary nucleotide sequence differs from that of the corresponding DNA sequence. This posttranscriptional mRNA editing consists almost exclusively of C-to-U substitutions (direct) and less frequently of U-to-C substitution (reverse). Editing occurs predominantly within coding regions, mostly at isolated C residues, and usually at first or second positions of codons, thereby almost always changing the amino acid from that specified by the unedited codon. Editing may also create initiation and termination codons. The effect of C-to-U RNA editing in plants is to make proteins encoded by plant organelles more similar in sequence to their non plant homologs, then specific C-to- U editing events are essential for the production of functional plant mitochondrial proteins. Our attention has been devoted to the study of the mRNA editing in cox3 mitochondrial gene of fern Asplenium nidus. This study reveals the extreme importance of both C-to-U and U-to-C substitutions for protein expression.
文摘Asplenium nidus L. commonly called as Bird’s Nest Fern, is a threatened, ornamental fern, which is widely used as novel foliage ornamental plant and local people use it in worship. The taxon is threatened due to over exploitation, habitat destruction and genetic barriers. To understand the constraints in the regeneration, reproductive biology studies are made. It is observed that more sporophytes are produced in composite population (13.3%) in comparison to isolate population (10%). This pattern is suggestive of the fact that the parental sporophyte is heterozygous for recessive sporophytic lethal. On the basis of the results obtained A. nidus was initially adapted for outbreeding with the capacity for considerable amount of inbreeding. The low potential of sporophyte production in isolate gametophyte could be the constitution of the zygotic genotype.
基金Supported by Youth Science Fund Project of Heilongjiang Province(QC2009C64)
文摘[Objective] The paper was to explore the oviposition site selection of Anoplophora glabripennis in sugar maple and its gnawing nidus oviposition habits. [Method] Through field and indoor rearing observation, the oviposition site selection of A. glabripennis in sugar maple was studied, and the gnawing nidus oviposition habits were analyzed. [Result] The height of oviposition site of A. glabripennis in sugar maple, the number observation holes （gnawing nidus oviposition points） and bark thickness in oviposition site showed linear correlation with diameter of branch at breast height. The gnawing nidus oviposition habit of A. glabripennis in sugar maple was mainly divided into 4 stages： gnawing nidus site selection, gnawing nidus, oviposition and nidus sealing, the duration from gnawing nidus to nidus sealing was（38.29±16.47）min. [Conclusion] The study provided reference for comprehensive prevention of A. glabripennis in greening tree species sugar maple, as well as further study on reproductive behavior and regulation mechanism of A. glabripennis.
