A hypothesis was formulated to explain the possible cause of water bloom occurring inLake Qiandaohu in 1998 and 1999. We tested this hypothesis with a 3-year in situ fieldstudy. The results showed that the reconstruct...A hypothesis was formulated to explain the possible cause of water bloom occurring inLake Qiandaohu in 1998 and 1999. We tested this hypothesis with a 3-year in situ fieldstudy. The results showed that the reconstruction of the silver carp and bighead carppopulations, without other measures of nutrient control, could prevent the recurrence ofalgal bloom in the lake successfully. This result could serve as an evidence to thesuggested hypothesis for water blooming: The drastic decline of the filter feedingsilver carp and bighead carp in the lake, rather than the nutrients overloading, wasmainly responsible for the algal bloom. According to this study, we suggest a generalhypothesis to the ecological mechanism of algal blooming: The insufficient grazing fromthe phytoplanktivores (top-down control) to the algal reproduction from nutrientsavailable (bottom-up effect) is the radical cause of water blooming, while conventionally,it is primarily attributed to the enrichment of nutrients. Besides, this study showedthat stocking silver carp and bighead carp in lakes could improve water quality, whichis also contrary to the conventional opinion. Finally, this study provided a cost-effective and practicable approach to control water bloom for the large-sized reservoirs,especially when water blooming occurred locally. A net-enclosed aquaculture zone (NEAZ)can be established in the nutrients-exposure area of the waters and stocked with the twocarps, water bloom could be controlled and prevented.展开更多
Based on the remote sensing images of algae, the present work analyzes the horizontal distribution characteristics of algal blooms in Chaohu Lake, China, which also reveals the frequency of algal blooms under differen...Based on the remote sensing images of algae, the present work analyzes the horizontal distribution characteristics of algal blooms in Chaohu Lake, China, which also reveals the frequency of algal blooms under different wind directions. Further, an unstructured-grid, three-dimensional finite-volume coastal ocean model （FVCOM） is applied to investigate the wind-induced currents and the transport pro- cess to explain the reason why algal blooms occur at the detected places. We first deduce the primary distribution of biomass from overlaid satellite images, and explain the formation mechanism by analyzing the pollution sources, and simulating the flow field and transportation process under prevailing wind over Chaohu Lake. And then, we consider the adjustment action of the wind on the corresponding day and develop a two-time scale approach to describe the whole formation process of algae horizontal distribution in Chaohu Lake. That is, on the longer time scale, i.e., during bloom season, prevailing wind determines the primary distribution of biomass by inducing the characteristic flow field; on the shorter time scale, i.e., on the day when bloom occurs, the wind force adjusts the primary distribution of biomass to form the final distribution of algal bloom.展开更多
Background:Sperm abnormalities are one of the primary factors leading to male sterility,but their pathogenesis is still unclear.Although miRNAs are suggested to exert important roles in the regulation of spermatogenes...Background:Sperm abnormalities are one of the primary factors leading to male sterility,but their pathogenesis is still unclear.Although miRNAs are suggested to exert important roles in the regulation of spermatogenesis at both transcriptional and posttranscriptional levels,little is currently known regarding the regulation of sperm flagella assembly by microRNAs(miRNAs).The role of miRNAs in the development of sperm abnormalities in sterile triploid fish has not been studied.Results:In this study,we found that miR-199-5p was widely expressed in all detected tissues of different-ploidy crucian carp.As one of the testis-specific candidate markers,Tekt1 was predominantly expressed in the testis.Quantitative real-time PCR(qRT-PCR)analyses showed that the expression trend of miR-199-5p was exactly opposite to that of Tekt1.Through bioinformatics analysis,we identified a putative miR-199-5p binding site in the Tekt1 mRNA.We further identified Tekt1 as a target of miR-199-5p using luciferase reporter assay.Finally,we confirmed that miR-199-5p was necessary for sperm flagellar assembly and spermatogenesis in vivo via intraperitoneal injection of miR-199-5p antagomir or agomir in diploid red crucian carp.Moreover,miR-199-5p gainof-function could lead to spermatids apoptosis and abnormal spermatozoa structure,which is similar to that of allotriploid crucian carp.Conclusions:Our studies suggested that abnormally elevated miR-199-5p inhibited the sperm flagella formation in spermiogenesis by negatively regulating the expression of Tekt1,thereby causing sperm abnormalities of male allotriploid crucian carp.展开更多
Recent development in the use of the environmental radionuclide caesium-137 for documenting rates and soil redistribution on the cultivated or uncultivated land and estimating rates of sediment deposition on represent...Recent development in the use of the environmental radionuclide caesium-137 for documenting rates and soil redistribution on the cultivated or uncultivated land and estimating rates of sediment deposition on represents an important advance that overcomes many of the limitations of the conventional techniques commonly applied in such investigations. A study on soil redistribution （including soil erosion and deposition） was carried out in the Dian Lake catchment, Yunnan Province, using ^137Cs and selected chemical properties. The average soil erosion rate was 1,280.2 t km^2 yr^-1. Soil erosion rate occurring on different parts of the slope was significantly different on different parts of the slope, increasing.from the top. the bottom to the middle slope. The average soil erosion rate is also different with the land use type and that of the cultivated land （1, 672. 8 t km^-2 yr^-1） is higher than oJ the uncultivated land （1.161.2t km^-2 yr^-1 ）. The result shows that landform, slope gradient and land use type are key factors that influence the size of soil erosion. In addition, we also find the SOC and TN contents and amount of the soil erosion to be correlated in the soil. With the soil erosion occurring, there are land degradation and the local eco-environmental problems, such as water eutrophication in Dian Lake.展开更多
A preliminary study on the advance and the retreat of Qingtu Lake and its climatic records of the Holocene lacustrine deposits has been made through comprehensive analysis. The study shows that the modes characteristi...A preliminary study on the advance and the retreat of Qingtu Lake and its climatic records of the Holocene lacustrine deposits has been made through comprehensive analysis. The study shows that the modes characteristics of climatic change in Eastern Hexi Corridor was provided with the corresponding relationship of warm moist and cold dry during the scale longer than hundred years. As a result of climatic changes and human activities, Qingtu Lake cycle has undergone four lake retreated and three lake advanced stages since 6000 a B.P. Based on the study on lacustrine sedimentary color, grain size, Fe 3+ /Fe 2+ and organic matter, the authors put forward that a warm period existed during the phase of 335-480 A.D. in the studied area. This warm period could be further testified by a lot of evidences obtained from the historical documents and the natural records of Qingtu Lake. In brief, the evolution of lakes in Eastern Hexi Corridor is characterized by sedimentary continuity, fast sedimentary rate and high resolution, it not only indicate the paleoenvironmental and climatic change in Holocene, but also can reflect the intensity of Eastern Asia monsoon.展开更多
The molecular dynamics simulation method was adopted to study the transient characteristics of Li^+,CO3^2-,and SO4^2- in Na^+,K^+,Li^+,Cl^-,and SO4^2-/H2O system.The composition of Na^+,K^+,Li^+,Cl^-,SO4^2- and CO3^2-...The molecular dynamics simulation method was adopted to study the transient characteristics of Li^+,CO3^2-,and SO4^2- in Na^+,K^+,Li^+,Cl^-,and SO4^2-/H2O system.The composition of Na^+,K^+,Li^+,Cl^-,SO4^2- and CO3^2- was selected to optimize the initial structural model and conduct dynamic simulation.The mean azimuth shift and diffusion coefficient of Li^+,CO3^2-,and SO4^2- in the system,the radial distribution function and potential energy between Li^+ and -OW,SO4^2- and -OW as well as CO3^2- and -OW,and the dielectric constant of hydrogen bond were expounded and analyzed.At the same time,the Li enrichment behavior in the evaporation process of salt lake brine was analyzed based on the simulated data.The results show that the simulation results are in good agreement with the experimental values,which verifies that,compared with other ions,the crystallization of Li^+ and SO4^2- occurs earlier after reaching saturation.展开更多
基金supported by the Nationa1 Natural Science Foundation of China(30028018).
文摘A hypothesis was formulated to explain the possible cause of water bloom occurring inLake Qiandaohu in 1998 and 1999. We tested this hypothesis with a 3-year in situ fieldstudy. The results showed that the reconstruction of the silver carp and bighead carppopulations, without other measures of nutrient control, could prevent the recurrence ofalgal bloom in the lake successfully. This result could serve as an evidence to thesuggested hypothesis for water blooming: The drastic decline of the filter feedingsilver carp and bighead carp in the lake, rather than the nutrients overloading, wasmainly responsible for the algal bloom. According to this study, we suggest a generalhypothesis to the ecological mechanism of algal blooming: The insufficient grazing fromthe phytoplanktivores (top-down control) to the algal reproduction from nutrientsavailable (bottom-up effect) is the radical cause of water blooming, while conventionally,it is primarily attributed to the enrichment of nutrients. Besides, this study showedthat stocking silver carp and bighead carp in lakes could improve water quality, whichis also contrary to the conventional opinion. Finally, this study provided a cost-effective and practicable approach to control water bloom for the large-sized reservoirs,especially when water blooming occurred locally. A net-enclosed aquaculture zone (NEAZ)can be established in the nutrients-exposure area of the waters and stocked with the twocarps, water bloom could be controlled and prevented.
基金supported by the Hundred Talents Program of Chinese Academy of Sciencesthe National Natural Science Fundation of China for Distinguished Young Scholar(10825211)
文摘Based on the remote sensing images of algae, the present work analyzes the horizontal distribution characteristics of algal blooms in Chaohu Lake, China, which also reveals the frequency of algal blooms under different wind directions. Further, an unstructured-grid, three-dimensional finite-volume coastal ocean model （FVCOM） is applied to investigate the wind-induced currents and the transport pro- cess to explain the reason why algal blooms occur at the detected places. We first deduce the primary distribution of biomass from overlaid satellite images, and explain the formation mechanism by analyzing the pollution sources, and simulating the flow field and transportation process under prevailing wind over Chaohu Lake. And then, we consider the adjustment action of the wind on the corresponding day and develop a two-time scale approach to describe the whole formation process of algae horizontal distribution in Chaohu Lake. That is, on the longer time scale, i.e., during bloom season, prevailing wind determines the primary distribution of biomass by inducing the characteristic flow field; on the shorter time scale, i.e., on the day when bloom occurs, the wind force adjusts the primary distribution of biomass to form the final distribution of algal bloom.
基金supported by the National Natural Science Foundation of China(Grant No.31872551)Laboratory of Lingnan Modern Agriculture Project(Grant No.NT2021008)+4 种基金the Natural Science Foundation of Hunan Province for Distinguished Young Scholars(Grant No.2020JJ2022)Hunan Provincial Innovation Foundation for Postgraduate(Grant No.CX20200480)the earmarked fund for China Agriculture Research System(Grant No.CARS-45)the Key Research and Development Program of Hunan Province(Grant No.2020NK2016)111 Project(D20007).
文摘Background:Sperm abnormalities are one of the primary factors leading to male sterility,but their pathogenesis is still unclear.Although miRNAs are suggested to exert important roles in the regulation of spermatogenesis at both transcriptional and posttranscriptional levels,little is currently known regarding the regulation of sperm flagella assembly by microRNAs(miRNAs).The role of miRNAs in the development of sperm abnormalities in sterile triploid fish has not been studied.Results:In this study,we found that miR-199-5p was widely expressed in all detected tissues of different-ploidy crucian carp.As one of the testis-specific candidate markers,Tekt1 was predominantly expressed in the testis.Quantitative real-time PCR(qRT-PCR)analyses showed that the expression trend of miR-199-5p was exactly opposite to that of Tekt1.Through bioinformatics analysis,we identified a putative miR-199-5p binding site in the Tekt1 mRNA.We further identified Tekt1 as a target of miR-199-5p using luciferase reporter assay.Finally,we confirmed that miR-199-5p was necessary for sperm flagellar assembly and spermatogenesis in vivo via intraperitoneal injection of miR-199-5p antagomir or agomir in diploid red crucian carp.Moreover,miR-199-5p gainof-function could lead to spermatids apoptosis and abnormal spermatozoa structure,which is similar to that of allotriploid crucian carp.Conclusions:Our studies suggested that abnormally elevated miR-199-5p inhibited the sperm flagella formation in spermiogenesis by negatively regulating the expression of Tekt1,thereby causing sperm abnormalities of male allotriploid crucian carp.
基金sponsored by the fund on soil ero-sion and silt source of Dian Lake catchment (Grant No. 40473052).
文摘Recent development in the use of the environmental radionuclide caesium-137 for documenting rates and soil redistribution on the cultivated or uncultivated land and estimating rates of sediment deposition on represents an important advance that overcomes many of the limitations of the conventional techniques commonly applied in such investigations. A study on soil redistribution （including soil erosion and deposition） was carried out in the Dian Lake catchment, Yunnan Province, using ^137Cs and selected chemical properties. The average soil erosion rate was 1,280.2 t km^2 yr^-1. Soil erosion rate occurring on different parts of the slope was significantly different on different parts of the slope, increasing.from the top. the bottom to the middle slope. The average soil erosion rate is also different with the land use type and that of the cultivated land （1, 672. 8 t km^-2 yr^-1） is higher than oJ the uncultivated land （1.161.2t km^-2 yr^-1 ）. The result shows that landform, slope gradient and land use type are key factors that influence the size of soil erosion. In addition, we also find the SOC and TN contents and amount of the soil erosion to be correlated in the soil. With the soil erosion occurring, there are land degradation and the local eco-environmental problems, such as water eutrophication in Dian Lake.
文摘A preliminary study on the advance and the retreat of Qingtu Lake and its climatic records of the Holocene lacustrine deposits has been made through comprehensive analysis. The study shows that the modes characteristics of climatic change in Eastern Hexi Corridor was provided with the corresponding relationship of warm moist and cold dry during the scale longer than hundred years. As a result of climatic changes and human activities, Qingtu Lake cycle has undergone four lake retreated and three lake advanced stages since 6000 a B.P. Based on the study on lacustrine sedimentary color, grain size, Fe 3+ /Fe 2+ and organic matter, the authors put forward that a warm period existed during the phase of 335-480 A.D. in the studied area. This warm period could be further testified by a lot of evidences obtained from the historical documents and the natural records of Qingtu Lake. In brief, the evolution of lakes in Eastern Hexi Corridor is characterized by sedimentary continuity, fast sedimentary rate and high resolution, it not only indicate the paleoenvironmental and climatic change in Holocene, but also can reflect the intensity of Eastern Asia monsoon.
基金Funded by the Innovation Academy for Green Manufacture,CAS“IAGM2020C01”the Key R&D and the Transformation Projects in Qinghai Province(2019-GX-167)CAS“Light of West China”。
文摘The molecular dynamics simulation method was adopted to study the transient characteristics of Li^+,CO3^2-,and SO4^2- in Na^+,K^+,Li^+,Cl^-,and SO4^2-/H2O system.The composition of Na^+,K^+,Li^+,Cl^-,SO4^2- and CO3^2- was selected to optimize the initial structural model and conduct dynamic simulation.The mean azimuth shift and diffusion coefficient of Li^+,CO3^2-,and SO4^2- in the system,the radial distribution function and potential energy between Li^+ and -OW,SO4^2- and -OW as well as CO3^2- and -OW,and the dielectric constant of hydrogen bond were expounded and analyzed.At the same time,the Li enrichment behavior in the evaporation process of salt lake brine was analyzed based on the simulated data.The results show that the simulation results are in good agreement with the experimental values,which verifies that,compared with other ions,the crystallization of Li^+ and SO4^2- occurs earlier after reaching saturation.
