With 200 mmol/L NaCl treatment on barley cultivar 'Jian 4' (Hordeum vulgare L. cv. J4) seedlings for 6 d, the contents of covalently and noncovalently conjugated polyamines (PAs) and activities of H+-ATPase in...With 200 mmol/L NaCl treatment on barley cultivar 'Jian 4' (Hordeum vulgare L. cv. J4) seedlings for 6 d, the contents of covalently and noncovalently conjugated polyamines (PAs) and activities of H+-ATPase in plasma membrane (PM) vesicles isolated from the roots decreased remarkably. Moreover, the activity of Na+/H+ antiport was detected first in PM vesicles. The results showed that the decrease in the contents of membrane phospholipid, noncovalently conjugated PAs and activity of H+-ATPase caused by NaCl could be restored partially by application of 1 mmol/L stearic acid (C16:0) and linoleic acid (C18:2), and C18:2 was more effective than C16:0. In addition, a reduction in the contents of covalently conjugated PAs was only reversed partially in the presence of C18:2. Furthermore, Na+/H+ antiport activity was strengthened by exogenous C16:0 and C18:2, and C18:2 was more effective than C16:0. The correlative analysis suggested that, after application of C16:0 and C18:2 under salt stress, there was a展开更多
基金We are grateful to Prof. Huang Huacheng (School of Academic Science, Shandong Agricultural University) for his useful suggestions This work is a part of post doctoral work of the author.
文摘With 200 mmol/L NaCl treatment on barley cultivar 'Jian 4' (Hordeum vulgare L. cv. J4) seedlings for 6 d, the contents of covalently and noncovalently conjugated polyamines (PAs) and activities of H+-ATPase in plasma membrane (PM) vesicles isolated from the roots decreased remarkably. Moreover, the activity of Na+/H+ antiport was detected first in PM vesicles. The results showed that the decrease in the contents of membrane phospholipid, noncovalently conjugated PAs and activity of H+-ATPase caused by NaCl could be restored partially by application of 1 mmol/L stearic acid (C16:0) and linoleic acid (C18:2), and C18:2 was more effective than C16:0. In addition, a reduction in the contents of covalently conjugated PAs was only reversed partially in the presence of C18:2. Furthermore, Na+/H+ antiport activity was strengthened by exogenous C16:0 and C18:2, and C18:2 was more effective than C16:0. The correlative analysis suggested that, after application of C16:0 and C18:2 under salt stress, there was a
