目的:观察七龙天对低氧性肺动脉高压(HPH)大鼠内皮素-1(ET-1)、血管内皮细胞一氧化氮合成酶(NOS)的影响。方法:雄性SD大鼠随机分为6组:空白对照组(A)、模型组(B)、波生坦组(C)、七龙天低剂量组(D)、七龙天中剂量组(E)、七龙天高剂量组(F...目的:观察七龙天对低氧性肺动脉高压(HPH)大鼠内皮素-1(ET-1)、血管内皮细胞一氧化氮合成酶(NOS)的影响。方法:雄性SD大鼠随机分为6组:空白对照组(A)、模型组(B)、波生坦组(C)、七龙天低剂量组(D)、七龙天中剂量组(E)、七龙天高剂量组(F)。A组大鼠置于正常环境中饲养,B、C、D、E、F组均造模。采用常压低氧法制模,从制模第1天始给药,连续3周,A、B两组均给予蒸馏水3 m L/(只·d)。在直视下行右心导管术测定肺血流动力学参数,及RT-PCR法检测肺组织中NOS、ET-1 mRNA表达。结果:七龙天及波生坦均可明显降低大鼠肺动脉高压,且以七龙天高剂量组效果为佳,疗效呈药物剂量依赖表现。各组NOS、ET-1表达量与肺动脉压具有相关性,但二者在高剂量七龙天调节至正常范围时,肺动脉压仍未恢复正常水平。结论:不同剂量七龙天均可降低肺动脉高压,且高剂量七龙天疗效优于波生坦。其降压机制与降低NOS及ET-1表达量有关,但仍可能受其他炎症因子及血管新生因子影响。展开更多
的 在新生鼠缺氧性脑损伤的模型基础上 ,测定损伤脑组织细胞内诱生型一氧化氮合酶 (i NOS)与结构型一氧化氮合酶 (c NOS)的 m RNA表达变化 ,以期阐明它们在发病中的作用。方法 取新生 SD鼠随机分组制作模型并行病理鉴定 ;采用 RT- PC...的 在新生鼠缺氧性脑损伤的模型基础上 ,测定损伤脑组织细胞内诱生型一氧化氮合酶 (i NOS)与结构型一氧化氮合酶 (c NOS)的 m RNA表达变化 ,以期阐明它们在发病中的作用。方法 取新生 SD鼠随机分组制作模型并行病理鉴定 ;采用 RT- PCR测定脑缺氧时脑组织细胞内 i NOS m RNA、c NOS m RNA表达的变化。辉度扫描获取数值。结果 鼠缺氧 1h后脑组织中 c NOS m RNA表达显著上升 (P<0 .0 1) ,4 h组较前下降 (P<0 .0 1) ,但仍显著高于对照组 (P<0 .0 1)。鼠缺氧 1h后脑组织中 i NOS m RNA表达与对照组相比有所上升 ,但无显著差异 (P>0 .0 5 ) ,4 h组表达明显上升 (P<0 .0 1) ,与前 2组相比有显著差别 (P<0 .0 1)。结论 脑缺氧早期 c NOS m RNA表达增加 ,由其合成的 NO对受损脑组织可能起保护作用 ;晚期i NOS m RNA表达明显增加 ,其所产生的展开更多
Objective The pathological significance of nitric oxide (NO) in the intestinal type radiation sickness. Methods: The intestinal type radiation sickness of BALB/c mice were induced byCo γ-radiation, andthe nitric oxid...Objective The pathological significance of nitric oxide (NO) in the intestinal type radiation sickness. Methods: The intestinal type radiation sickness of BALB/c mice were induced byCo γ-radiation, andthe nitric oxide synthase (NOS), mucosa structure and survival rate of intestinal gland were examined by using NADPH-diaphorase histochemistry, H-E staining, Feulgen’s reaction and intestinal gland counting techniques. Results: Compared with irradiated mice without (L--NAME ) treatment, the dilatation of intestinalcavity and blood vessels of mice treated with L-NAME were lessened, the survival rates of intestinal glandwere remarkably increased (P < 0. 01), the mucosal structure was obviously improved, and the amount ofNOS--positive structures or products were also reduced. Conclusion: Activation of NOS or increase of NOsynthesis might aggravate the acute pathological injuries of intestines of the irradiated mice, whereas administration of L-NAME has protective effect on the intestinal mucosa to certain extent.展开更多
文摘目的:观察七龙天对低氧性肺动脉高压(HPH)大鼠内皮素-1(ET-1)、血管内皮细胞一氧化氮合成酶(NOS)的影响。方法:雄性SD大鼠随机分为6组:空白对照组(A)、模型组(B)、波生坦组(C)、七龙天低剂量组(D)、七龙天中剂量组(E)、七龙天高剂量组(F)。A组大鼠置于正常环境中饲养,B、C、D、E、F组均造模。采用常压低氧法制模,从制模第1天始给药,连续3周,A、B两组均给予蒸馏水3 m L/(只·d)。在直视下行右心导管术测定肺血流动力学参数,及RT-PCR法检测肺组织中NOS、ET-1 mRNA表达。结果:七龙天及波生坦均可明显降低大鼠肺动脉高压,且以七龙天高剂量组效果为佳,疗效呈药物剂量依赖表现。各组NOS、ET-1表达量与肺动脉压具有相关性,但二者在高剂量七龙天调节至正常范围时,肺动脉压仍未恢复正常水平。结论:不同剂量七龙天均可降低肺动脉高压,且高剂量七龙天疗效优于波生坦。其降压机制与降低NOS及ET-1表达量有关,但仍可能受其他炎症因子及血管新生因子影响。
文摘的 在新生鼠缺氧性脑损伤的模型基础上 ,测定损伤脑组织细胞内诱生型一氧化氮合酶 (i NOS)与结构型一氧化氮合酶 (c NOS)的 m RNA表达变化 ,以期阐明它们在发病中的作用。方法 取新生 SD鼠随机分组制作模型并行病理鉴定 ;采用 RT- PCR测定脑缺氧时脑组织细胞内 i NOS m RNA、c NOS m RNA表达的变化。辉度扫描获取数值。结果 鼠缺氧 1h后脑组织中 c NOS m RNA表达显著上升 (P<0 .0 1) ,4 h组较前下降 (P<0 .0 1) ,但仍显著高于对照组 (P<0 .0 1)。鼠缺氧 1h后脑组织中 i NOS m RNA表达与对照组相比有所上升 ,但无显著差异 (P>0 .0 5 ) ,4 h组表达明显上升 (P<0 .0 1) ,与前 2组相比有显著差别 (P<0 .0 1)。结论 脑缺氧早期 c NOS m RNA表达增加 ,由其合成的 NO对受损脑组织可能起保护作用 ;晚期i NOS m RNA表达明显增加 ,其所产生的
文摘Objective The pathological significance of nitric oxide (NO) in the intestinal type radiation sickness. Methods: The intestinal type radiation sickness of BALB/c mice were induced byCo γ-radiation, andthe nitric oxide synthase (NOS), mucosa structure and survival rate of intestinal gland were examined by using NADPH-diaphorase histochemistry, H-E staining, Feulgen’s reaction and intestinal gland counting techniques. Results: Compared with irradiated mice without (L--NAME ) treatment, the dilatation of intestinalcavity and blood vessels of mice treated with L-NAME were lessened, the survival rates of intestinal glandwere remarkably increased (P < 0. 01), the mucosal structure was obviously improved, and the amount ofNOS--positive structures or products were also reduced. Conclusion: Activation of NOS or increase of NOsynthesis might aggravate the acute pathological injuries of intestines of the irradiated mice, whereas administration of L-NAME has protective effect on the intestinal mucosa to certain extent.
