This experiment examined the fluctuations in nitrogen gas supersaturation throughout the day in three karst springs (upper, side, and lower) at McNenny State Fish Hatchery, rural Spearfish, Lawrence County, South Dako...This experiment examined the fluctuations in nitrogen gas supersaturation throughout the day in three karst springs (upper, side, and lower) at McNenny State Fish Hatchery, rural Spearfish, Lawrence County, South Dakota, USA. Total gas pressures, oxygen percent saturation, and nitrogen percent saturation were recorded six times/day on eight days over a 26-day period in each of the three springs. Total gas pressure did not vary significantly throughout the day in any of the springs. However, percent oxygen and nitrogen saturation were significantly different throughout the day in all three springs. The highest mean (SE) nitrogen supersaturation value of 118.5 (1.1)% was observed in the lower spring at 07:00. The lowest mean nitrogen supersaturation values were 114.5 (1.1)% at 13:00 in the upper spring, and 114.2 (0.2)% and 113.1 (0.7)% at 15:00 in the side and lower spring, respectively. At 118% nitrogen supersaturation, gas bubble disease is likely to occur in fish, resulting in potentially high levels of mortality if untreated spring water was used for fish production. The results of this study indicate the importance of recording nitrogen gas levels at sunrise or early in the morning, when nitrogen is highest and oxygen is lowest, to obtain accurate and reproducible data.展开更多
Spring maize is one of the most popular crops planted in northeastem China. The cropping systems involving spring maize have been maintaining high production through intensive management practices. However, the high r...Spring maize is one of the most popular crops planted in northeastem China. The cropping systems involving spring maize have been maintaining high production through intensive management practices. However, the high rates of nitrogen (N) fertilizers application could have introduced a great amount of nitrous oxide (N2O) into the atmosphere. It is crucial for sustaining the maize production systems to reduce N2O emissions meanwhile maintaining the optimum yields by adopting alternative farming management practices. The goal of this study was to evaluate effects of alternative fertilization and crop residue management practices on N2O emission as well as crop yield for a typical maize field in northeastern China. Field experiments were conducted during the 2010-2011 maize growing seasons (from early May to late September) in Liaoning Province, northeastern China. N2O fluxes were measured at the field plots with six different treatments including no N fertilizer use (CK), farmers' conventional N fertilizer application rate (FP), reduced N fertilizer rate (OPT), reduced N fertilizer rate combined with crop straw amendment (OPTS), slow-release N fertilizer (CRF), and reduced N fertilizer rate combined with nitrification inhibitor (OPT+DCD). The static chamber method combined with gas chromatography technique was employed to conduct the measurements of N2O fluxes. The field data showed that N2O emissions varied across the treatments. During the maize growing season in 2010, the total N2O emissions under the treatments of CK, FP, OPT, OPTS, and CRF were 0.63, 1.11, 1.03, 1.26, and 0.98 kg N ha-1, respectively. The seasonal cumulative N2O emissions were 0.54, 1.07, 0.96, 1.12, and 0.84 kg N ha1, respectively, under CK, FP, OPT, OPTS, and OPT+DCD in 2011. In comparison with FP, CRF or OPT+DCD reduced the N2O emissions by 12 or 21%, respectively, while the crop yields remained unchanged. The results indicate that the reduction of N-fertilizer application rate in combination with the slow-release fertilizer type or nitrification inhibitor could effectively mitigate N2O emissions from the tested field. The incorporation of crop residue didn't show positive effect on mitigating N2O emissions from the tested cropping system. The field study can provide useful information for the on-going debate on alternative N fertilization strategies and crop straw management in China. However, further studies would be needed to explore the long-term impacts of the alternative management practices on a wide range of environmental services.展开更多
Gliadin, the major storage protein in endosperm, affects grain quality in spring wheat by its content and composition. Eighteen cultivars differing in HMW-GS were used in the study to approach the accumulation pattern...Gliadin, the major storage protein in endosperm, affects grain quality in spring wheat by its content and composition. Eighteen cultivars differing in HMW-GS were used in the study to approach the accumulation pattern of gliadin fractions α, β, γ, ω and regulation of three kinds of nitrogen source. The results showed that the content of gliadin in grains increased gradually along with the process of grain-filling, but the accumulation intensity and final content differed evidently among cultivars with different HMW-GS composition. Of all the subunit types used here, cultivars with subunits 7+9 and 2+12 had smaller accumulation intensity and lower final content. During grain-filling, 4 gliadin fractions had the same increase trend, but differed in accumulation course. The dynamic trends of gliadin accumulation were similar in different nitrogen treatments whose effects on initial amount, accumulation intensity and final level of accumulation varied with cultivars. Of three nitrogen fertilizer types, the amide-form nitrogen source was better to the formation and accumulation of gliadin as well as its four fractions.展开更多
文摘This experiment examined the fluctuations in nitrogen gas supersaturation throughout the day in three karst springs (upper, side, and lower) at McNenny State Fish Hatchery, rural Spearfish, Lawrence County, South Dakota, USA. Total gas pressures, oxygen percent saturation, and nitrogen percent saturation were recorded six times/day on eight days over a 26-day period in each of the three springs. Total gas pressure did not vary significantly throughout the day in any of the springs. However, percent oxygen and nitrogen saturation were significantly different throughout the day in all three springs. The highest mean (SE) nitrogen supersaturation value of 118.5 (1.1)% was observed in the lower spring at 07:00. The lowest mean nitrogen supersaturation values were 114.5 (1.1)% at 13:00 in the upper spring, and 114.2 (0.2)% and 113.1 (0.7)% at 15:00 in the side and lower spring, respectively. At 118% nitrogen supersaturation, gas bubble disease is likely to occur in fish, resulting in potentially high levels of mortality if untreated spring water was used for fish production. The results of this study indicate the importance of recording nitrogen gas levels at sunrise or early in the morning, when nitrogen is highest and oxygen is lowest, to obtain accurate and reproducible data.
基金the National Natural Science Foundation of China(31270486)the Special Fund for Agriculture Scientific Non-Profit Research of China(201103039)+1 种基金the National Basic Research Program of China(2012CB417104)the National Nonprofit Institute Research Grant of Chinese Academy of Agricultural Sciences(IARRP-2014-402-15)
文摘Spring maize is one of the most popular crops planted in northeastem China. The cropping systems involving spring maize have been maintaining high production through intensive management practices. However, the high rates of nitrogen (N) fertilizers application could have introduced a great amount of nitrous oxide (N2O) into the atmosphere. It is crucial for sustaining the maize production systems to reduce N2O emissions meanwhile maintaining the optimum yields by adopting alternative farming management practices. The goal of this study was to evaluate effects of alternative fertilization and crop residue management practices on N2O emission as well as crop yield for a typical maize field in northeastern China. Field experiments were conducted during the 2010-2011 maize growing seasons (from early May to late September) in Liaoning Province, northeastern China. N2O fluxes were measured at the field plots with six different treatments including no N fertilizer use (CK), farmers' conventional N fertilizer application rate (FP), reduced N fertilizer rate (OPT), reduced N fertilizer rate combined with crop straw amendment (OPTS), slow-release N fertilizer (CRF), and reduced N fertilizer rate combined with nitrification inhibitor (OPT+DCD). The static chamber method combined with gas chromatography technique was employed to conduct the measurements of N2O fluxes. The field data showed that N2O emissions varied across the treatments. During the maize growing season in 2010, the total N2O emissions under the treatments of CK, FP, OPT, OPTS, and CRF were 0.63, 1.11, 1.03, 1.26, and 0.98 kg N ha-1, respectively. The seasonal cumulative N2O emissions were 0.54, 1.07, 0.96, 1.12, and 0.84 kg N ha1, respectively, under CK, FP, OPT, OPTS, and OPT+DCD in 2011. In comparison with FP, CRF or OPT+DCD reduced the N2O emissions by 12 or 21%, respectively, while the crop yields remained unchanged. The results indicate that the reduction of N-fertilizer application rate in combination with the slow-release fertilizer type or nitrification inhibitor could effectively mitigate N2O emissions from the tested field. The incorporation of crop residue didn't show positive effect on mitigating N2O emissions from the tested cropping system. The field study can provide useful information for the on-going debate on alternative N fertilization strategies and crop straw management in China. However, further studies would be needed to explore the long-term impacts of the alternative management practices on a wide range of environmental services.
文摘Gliadin, the major storage protein in endosperm, affects grain quality in spring wheat by its content and composition. Eighteen cultivars differing in HMW-GS were used in the study to approach the accumulation pattern of gliadin fractions α, β, γ, ω and regulation of three kinds of nitrogen source. The results showed that the content of gliadin in grains increased gradually along with the process of grain-filling, but the accumulation intensity and final content differed evidently among cultivars with different HMW-GS composition. Of all the subunit types used here, cultivars with subunits 7+9 and 2+12 had smaller accumulation intensity and lower final content. During grain-filling, 4 gliadin fractions had the same increase trend, but differed in accumulation course. The dynamic trends of gliadin accumulation were similar in different nitrogen treatments whose effects on initial amount, accumulation intensity and final level of accumulation varied with cultivars. Of three nitrogen fertilizer types, the amide-form nitrogen source was better to the formation and accumulation of gliadin as well as its four fractions.